Articles by Sandesh Marathe:
6 Dos and Don’ts for Perfecting the CAM Assay
Don’t put all your eggs in one basket! Learn how to handle your eggs, prevent contamination and keep track of your experiments when performing the CAM assay.

PhD vs Master’s: Which to Choose?
If choosing between a PhD or a Master’s has you frazzled, don’t panic. We weigh the pros and cons of both in terms of time, money, and career prospects.

Chick Embryo: A Model System for Studying Angiogenesis
Are you studying angiogenesis? Is cell culture not cutting it but you’re not ready for an in vivo model? Check out the CAM assay.

Excelling With Excel: Analytical Method Validation Using LOD and LOQ
Don’t be fooled by bad data. Make sure your results are reliable with this quick guide to determining LOQ and LOD in Excel.

How to Maintain Motivation in the Lab During an Existential Crisis
How does one maintain the motivation to get out of the bed, do all the lab work, bear the supervisor and the colleagues one doesn’t gel with, and also maintain personal life? Here are a few ways to deal with stress and maintain motivation.

The Do’s and Don’ts of HPTLC
This followup article on HPTLC gives a list of dos and don’ts while performing HPTLC to achieve precise and error-free results, and avoid false positives.

HPTLC: Basics and Instrumentation
Get robotic with HPTLC! This automated version of TLC can separate and quantify compounds – find out how!

Troubleshooting Thin Layer Chromatography: Some TLC for Your TLC
The whole TLC technique sounds easy to do, but it can be difficult and tricky during interpretation or give unexpected results, especially when working with biomolecules. For this reason, it is important to be familiar with troubleshooting thin layer chromatography. Some of the common problems faced during TLC and their solutions are listed below: Solvent…

Thin Layer Chromatography: The Basics
Like most other chromatographic techniques, thin layer chromatography (TLC) separates out individual compounds from a mixture depending upon the polarity of each compound. The solvent system travels up a silica plate by capillary action and passes over the sample that you spot onto the plate. As the solvent travels up, it moves the compounds present…

The Many Uses of Thin Layer Chromatography
While working as biologists, we often come across mixtures of compounds, and the first question that strikes our minds is ‘what are the components in this mixture?’ One might think of using chemical assays to find the presence of specific compounds. But that sounds painful, doesn’t it? Well, the good news is that thin layer…
