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A Beginner’s Guide to The Point Spread Function

Learn how the Point Spread Function affects what you see through your microscope and discover what you can do to improve your images.
Ask Eric Anything
A live episode of The Microscopists featuring Eric Betzig

Ask Eric Anything: A live episode of The Microscopists featuring Eric Betzig On-Demand The Microscopists is going LIVE! For a one-off and very special guest – Eric Betzig! We already know so much about Eric, but I hope that our chat will reveal more about the man behind the many ingenious contributions to the world…
An introduction to Photoactivated Localization Microscopy (PALM)

How does photoactivated localization microscopy (PALM) work? And what use can PALM microscopy be to you? This short introduction to PALM gives you the answers!
Beginners Guide to PALM Sample Preparation

Good PALM sample preparation is the key to great images. Find out how to choose the right fluorescent proteins and learn some tips and tricks for sample prep.
Light sheet fluorescence microscopy for Multiview imaging of living and cleared specimens

Discover a new light sheet microscope for Multiview imaging of living and cleared specimens Now On-Demand Copyright Notice upper left: Courtesy of A. Mithoefer, Max-Planck-Institute for Chemical Ecology, Jena, Germany. upper right: Courtesy of T. Weiquan John, N. Huck Hui, Genome Institute of Singapore. bottom left: Sample courtesy of O. Efimova, National Research Center “Kurchatov…
The New ZEISS LSM 9 Family: Adding the Multiplex Factor to Your Confocal Imaging

The New ZEISS LSM 9 Family: Adding the Multiplex Factor to Your Confocal Imaging In this tutorial on confocal imaging, you will learn how you can: Capture weaker signals—and still get sound, reproducible data. Reach faster volumetric imaging without sacrificing resolution Increase data throughput for your imaging needs Speaker Joseph Huff Solution Manager/Application Development Engineer…
Revealing the fastest processes in superresolution – with Lattice SIM and ZEISS Elyra 7

Revealing the fastest processes in superresolution – with Lattice SIM and ZEISS Elyra 7 Speakers Renée M. Dalrymple Tutorial abstract Your life sciences research often requires you to measure, quantify and understand the finest details and sub-cellular structures of your sample. You may be working with tissue, bacteria, organoids, neurons, living or fixed -cells and…
New 2D Superresolution mode for ZEISS Airyscan – Fast and gentle confocal imaging with 120 nm resolution

New 2D Superresolution mode for ZEISS Airyscan – Fast and gentle confocal imaging with 120 nm resolution Speakers Joseph Huff, PhD Tutorial abstract Utilizing a pinhole-plane imaging concept, ZEISS Airyscan allows for simultaneous improvement in resolution and signal-to-noise by capitalizing on an innovative 32-channel GaAsP photomultiplier tube (PMT) array detector. Each detection channel functions as…
Biological Applications of X-Ray Microscopy and Correlative XRM – FIB-SEM Imaging

In this webinar, you will learn about the power of 3D imaging of larger samples. In particular, you will: Gain insight in to how to use x-ray microscopy to analyze biological samples Learn the basics about sample preparation for x-ray microscopy Understand the benefits and drawbacks to different imaging conditions X-Ray Microscopy (XRM) is a…
Mouse Whole-Brain Volume Electron Microscopy for Cellular Connectomics – Enabling Large-Scale SEM Projects with ZEISS MultiSEM

Recent advances in high-throughput multi-beam scanning electron microscopy (EM) and mouse whole-brain EM preparation and collection on tape (“Brain-on-Tape”) have resulted in substantial progress towards a nano-scale map of the whole mouse brain. These maps can be used to determine how individual neurons are synaptically connected and can be used to reconstruct the precise “wiring…
Automated Microscopy

The traditional microscope that you know and love is operated manually. Picture the scene: the microscopist chooses the light source, gently places the sample the moveable stage, selects the objective lens, and scans to select the field of view. This process is perfect for processing and analyzing a small number of samples per day. But…
Data Analysis for Three-dimensional Volume Scanning Electron Microscopy

In recent years, three-dimensional (3D) scanning electron microscopy techniques have gained recognition in the biological sciences. In particular, array tomography, serial block face scanning electron microscopy (SBFSEM) and focused ion beam scanning electron microscopy (FIBSEM) (described in Three-Dimensional Scanning Electron Microscopy for Biology) have shown an increase in biological applications, elucidating ultrastructural details of cells…
Three-Dimensional Scanning Electron Microscopy for Biology

Scanning electron microscopy (SEM) is a powerful technique, traditionally used for imaging the surface of cells, tissues and whole multicellular organisms (see An Introduction to Electron Microscopy for Biologists)(Fig. 1). While the resultant images appear to be three dimensional (3D), they actually contain no depth information. However, there are several SEM techniques that can obtain…
The Fast Module for ZEISS LSM 880 with Airyscan: Confocal Superresolution Imaging with Four Times the Speed and Improved Signal-to-Noise Ratio

First introduced in August 2014, the Airyscan detector from ZEISS represents a new detector concept for laser scanning microscopy (LSM) that enables a simultaneous resolution and signal-to-noise (SNR) increase over traditional LSM imaging. The Airyscan detector design substitutes the conventional LSM detector and pinhole scheme for an array of 32 sensitive GaAsP detector elements, arranged…
Producing Your own Digital Classroom with ZEISS: Starring Wi-Fi-enabled Microscopes and Mobile Apps

It’s hard to believe, but microscopy is nearly 400 years old. Since the early 1600s, scientists have sought new and improved ways to view microscopic images, record them and share them with eager students and colleagues. In 1846, major advances in microscope engineering began in the fledgling workshop of Carl Zeiss, culminating with the first…
Microscopy Education In The Digital Age: Upgrade Your University With A Digital Classroom

In this webinar, Silvia Zenner-Gellrich , Ph.D., product manager at ZEISS, will share: How to integrate your microscopes with digital technology How you can use the Labscope imaging app in the classroom The benefits of using connected microscopes in your classroom Abstract: Universities and schools alike are competing which each other for the best rankings…
Let There Be Light! Microscope Maintenance Part 2: Köhler Illumination

In Part 1 of these articles, you’ll have learnt about common microscope light sources and how to replace and align these correctly. In this article, we will discuss the importance of Köhler illumination and how to set up the microscope to achieve optimal imaging results. What is Köhler illumination? Before discussing this technique, let us…
Let There Be Light! Microscope Maintenance Part 1: Routine Care and Replacing Bulbs

Do you want the best imaging experience each time you use a microscope? Well, this is a rhetorical question, as we all desire that these delicate optical instruments are clean, free from immersion oil and correctly aligned. From the routine checking of slides, capturing images for presentations and publications, to diagnosing diseases using point-of-care microscopes,…
Enabling Connectomics with Multi-beam Scanning Electron Microscopy – The World’s Fastest SEM Just Got Even Faster

In this webinar, presented by Anna Lena Eberle, PhD, Neuroscience Specialist and Product Manager for ZEISS MultiSEM, you will learn: Challenges and relevance of 3D imaging for Connectomics 3D volume imaging with scanning electron microscopy methods Newest technologies for ultrastructural high-throughput imaging Abstract: Recent years have witnessed major progress in three-dimensional (3D) microscopy techniques for…
ZEISS Airyscan: A Brave New Microscopy World with Sharper Confocal Resolution

Confocal laser scanning microscopy is today’s standard method of obtaining detailed 2-D and 3-D information at the cellular level. The imaging technology has become popular in biomedical research because of its ability to produce precise, optically sectioned images, and address many types of samples and application demands. Over the past 25 years, improvements have been…
How to Troubleshoot Problems with Fluorescently Tagged Proteins

Using fluorescent proteins as imaging probes is a widespread and versatile technique in microscopy. You can use them in a wide range of living systems, from single cultured cells to complete organisms and animals. Fluorescently tagged proteins can be used to track and examine real-time localization, interactions and translocation of your protein of interest, as…
In Vivo and Cleared Tissue Imaging for Light-sheet Microscopy

Discover light sheet fluorescence microscopy (LSFM), a technique for imaging of live developing organisms and large cleared tissue samples. Light sheet microscopy uses a thin sheet of light for sample excitation and a second separate light path for image detection to sidestep image blur created by traditional epi-illumination techniques. The result is an optically sectioned…
ZEISS LSM 800 with Airyscan – Your Compact Confocal Power Pack

In this webinar you will learn: about the benefits, features and applications of LSM 800 with Airyscan – a new confocal microscope from ZEISS that combines laser scanning microscopy with Airyscanning. Abstract: Confocal laser scanning microscopy (CLSM) is the recognized standard for 3D fluorescence microscopy, complete with highly sensitive GaAsP detection and fast linear scanning.…
Live-Cell Imaging: Choosing the Right Technique

If you want to see in real time what is going on inside your cell then you should be performing live-cell imaging. Live-cell imaging techniques allow real-time examination of almost every aspect of cellular function under normal and experimental conditions. With all live-cell imaging experiments, the main challenges are to keep your cells alive and healthy…
3D Electron Microscopy for Life Sciences

Life science research increasingly demands multimodal imaging methods to obtain comprehensive data about dynamic events. Light microscopy (LM) provides cellular localization and distribution patterns of genetically labelled proteins, but lacks information about ultrastructural details. A prospect in modern microscopy is the potential to augment live cell fluorescent imaging with direct high resolution ultrastructural information provided…
Enjoy the full potential of confocal microscopy with ZEISS LSM 880 with Airyscan

If you want a 3D fluorescent image you need a confocal microscope. The only question remains, which confocal microscope should you use? ZEISS LSM 880 with Airyscan unlocks the full potential of confocal microscopy. It is an exceptionally flexible system that uses an array detector increasing your sensitivity, resolution and speed. ZEISS LSM 880 with…
ZEISS LSM 880 with Airyscan – Revolutionize Your Confocal Imaging

In this webinar you will learn About Airyscan and how it can be combined with Confocal Laser Scanning Microscopy The benefits, features and applications of Confocal LSM 880 with Airyscan – a new confocal microscope from ZEISS that combines Confocal laser scanning microscopy with Airyscanning. Click here to request an instrument demonstration for the LSM…