Zeiss

Light sheet fluorescence microscopy for Multiview imaging of living and cleared specimens

Discover a new light sheet microscope for Multiview imaging of living and cleared specimens Now On-Demand Copyright Notice upper left: Courtesy of A. Mithoefer, Max-Planck-Institute for Chemical Ecology, Jena, Germany. upper right: Courtesy of T. Weiquan John, N. Huck Hui, Genome Institute of Singapore. bottom left: Sample courtesy of O. Efimova, National Research Center “Kurchatov…
The New ZEISS LSM 9 Family: Adding the Multiplex Factor to Your Confocal Imaging

The New ZEISS LSM 9 Family: Adding the Multiplex Factor to Your Confocal Imaging In this tutorial on confocal imaging, you will learn how you can: Capture weaker signals—and still get sound, reproducible data. Reach faster volumetric imaging without sacrificing resolution Increase data throughput for your imaging needs Speaker Joseph Huff Solution Manager/Application Development Engineer…
Revealing the fastest processes in superresolution – with Lattice SIM and ZEISS Elyra 7

Revealing the fastest processes in superresolution – with Lattice SIM and ZEISS Elyra 7 Speakers Renée M. Dalrymple Tutorial abstract Your life sciences research often requires you to measure, quantify and understand the finest details and sub-cellular structures of your sample. You may be working with tissue, bacteria, organoids, neurons, living or fixed -cells and…
New 2D Superresolution mode for ZEISS Airyscan – Fast and gentle confocal imaging with 120 nm resolution

New 2D Superresolution mode for ZEISS Airyscan – Fast and gentle confocal imaging with 120 nm resolution Speakers Joseph Huff, PhD Tutorial abstract Utilizing a pinhole-plane imaging concept, ZEISS Airyscan allows for simultaneous improvement in resolution and signal-to-noise by capitalizing on an innovative 32-channel GaAsP photomultiplier tube (PMT) array detector. Each detection channel functions as…
Biological Applications of X-Ray Microscopy and Correlative XRM – FIB-SEM Imaging

In this webinar, you will learn about the power of 3D imaging of larger samples. In particular, you will: Gain insight in to how to use x-ray microscopy to analyze biological samples Learn the basics about sample preparation for x-ray microscopy Understand the benefits and drawbacks to different imaging conditions X-Ray Microscopy (XRM) is a…
Mouse Whole-Brain Volume Electron Microscopy for Cellular Connectomics – Enabling Large-Scale SEM Projects with ZEISS MultiSEM

Recent advances in high-throughput multi-beam scanning electron microscopy (EM) and mouse whole-brain EM preparation and collection on tape (“Brain-on-Tape”) have resulted in substantial progress towards a nano-scale map of the whole mouse brain. These maps can be used to determine how individual neurons are synaptically connected and can be used to reconstruct the precise “wiring…
Automated Microscopy

The traditional microscope that you know and love is operated manually. Picture the scene: the microscopist chooses the light source, gently places the sample the moveable stage, selects the objective lens, and scans to select the field of view. This process is perfect for processing and analyzing a small number of samples per day. But…
Data Analysis for Three-dimensional Volume Scanning Electron Microscopy

In recent years, three-dimensional (3D) scanning electron microscopy techniques have gained recognition in the biological sciences. In particular, array tomography, serial block face scanning electron microscopy (SBFSEM) and focused ion beam scanning electron microscopy (FIBSEM) (described in Three-Dimensional Scanning Electron Microscopy for Biology) have shown an increase in biological applications, elucidating ultrastructural details of cells…
Three-Dimensional Scanning Electron Microscopy for Biology

Scanning electron microscopy (SEM) is a powerful technique, traditionally used for imaging the surface of cells, tissues and whole multicellular organisms (see An Introduction to Electron Microscopy for Biologists)(Fig. 1). While the resultant images appear to be three dimensional (3D), they actually contain no depth information. However, there are several SEM techniques that can obtain…
The Fast Module for ZEISS LSM 880 with Airyscan: Confocal Superresolution Imaging with Four Times the Speed and Improved Signal-to-Noise Ratio

First introduced in August 2014, the Airyscan detector from ZEISS represents a new detector concept for laser scanning microscopy (LSM) that enables a simultaneous resolution and signal-to-noise (SNR) increase over traditional LSM imaging. The Airyscan detector design substitutes the conventional LSM detector and pinhole scheme for an array of 32 sensitive GaAsP detector elements, arranged…
Producing Your own Digital Classroom with ZEISS: Starring Wi-Fi-enabled Microscopes and Mobile Apps

It’s hard to believe, but microscopy is nearly 400 years old. Since the early 1600s, scientists have sought new and improved ways to view microscopic images, record them and share them with eager students and colleagues. In 1846, major advances in microscope engineering began in the fledgling workshop of Carl Zeiss, culminating with the first…
Microscopy Education In The Digital Age: Upgrade Your University With A Digital Classroom

In this webinar, Silvia Zenner-Gellrich , Ph.D., product manager at ZEISS, will share: How to integrate your microscopes with digital technology How you can use the Labscope imaging app in the classroom The benefits of using connected microscopes in your classroom Abstract: Universities and schools alike are competing which each other for the best rankings…
Let There Be Light! Microscope Maintenance Part 2: Köhler Illumination

In Part 1 of these articles, you’ll have learnt about common microscope light sources and how to replace and align these correctly. In this article, we will discuss the importance of Köhler illumination and how to set up the microscope to achieve optimal imaging results. What is Köhler illumination? Before discussing this technique, let us…