Quantcast
Skip to content

Analytical Chemistry and Chromatography Techniques

Advances to SYNTHIA™ Retrosynthesis Software and How They Have Accelerated Chemical Development at GlaxoSmithKline (GSK)

Advances to SYNTHIA™ Retrosynthesis Software and How They Have Accelerated Chemical Development at GlaxoSmithKline (GSK) Available On Demand John Jin Lim, MS Senior ScientistAPI ChemistryGlaxoSmithKline plc (GSK) John Lim (MS) leads the CASD Team at GSK Chemical Development, which uses computational tools, such as SYNTHIA™ retrosynthesis software, for the route selection process. He obtained his…

Read More

Analyte Binding Following Sample Filtration in HPLC

Analyte Binding Following Sample Filtration in HPLC Available On Demand Speaker Vivek Joshi Senior R&D ManagerApplications DevelopmentMilliporeSigma Vivek Joshi, PhD is an Applications and Market Adoption Senior Manager at MilliporeSigma. He develops new products and applications for products that span analytical workflows, molecular and protein science workflows, and cell culture workflows. He recently presented a…

Read More

The End of the Beginning for Retrosynthetic Planning

The End of the Beginning for Retrosynthetic Planning Significant advances in SYNTHIA™ opening doors for complex molecule synthesis and bench validation Available On-Demand Lindsey Rickershauser, Ph.D. Manager of Sales & MarketingCheminformatics TechnologiesMerck KGaA, Darmstadt, Germany Lindsey Hess Rickershauser, PhD, currently manages the sales and marketing for SYNTHIA™ in the Cheminformatics Technologies division of MilliporeSigma, a…

Read More

The Do’s and Don’ts of HPTLC 

This followup article on HPTLC gives a list of dos and don’ts while performing HPTLC to achieve precise and error-free results, and avoid false positives.

Read More

Should You Switch from Wet to Dried Blood Samples?

A Spot of History Most of the biomedical methods used started as a curiosity. Then the one-off gains a limited use, the technology then progresses until its use becomes widespread. Just think about the arch from the curious polished glass spheres, used by Antony Levnhook to look at animalcules, to modern microscopes. The same story…

Read More

Crash Course in Fourier Transform Infrared Spectroscopy

Fourier transform infrared spectroscopy (FTIR) is an extremely sensitive technique for measuring the absorption and intensity of electromagnetic radiation in the infrared region of the spectrum of either a solid, liquid or gas sample. You can use FTIR to: quantify unknown compounds identify unknown compounds study the detailed structured coordination of compounds How Does Fourier…

Read More

Applying Your MALDI Matrix Like a Boss

Matrix-assisted laser desorption/ionization (or simply, MALDI) is a type of mass spectrometry method. If you are new to mass spectrometry, check out our excellent primers, titled How Does Mass Spec Work and Get Out of Western Blot Hell: An intro to Mass Spec. Basically, however, mass spectrometry allows you to measure, sort, and quantify small…

Read More

HPLC Tubings and a $200.000.000 NASA Crash: Converting Between Measurement Systems

In an amazing TED-Ed video from Matt Anticole, the wild history of the metric system is explained as a very smart cartoon. Matt concluded his video with a rather positive message for the scientific community: “Nevertheless, the metric system is almost universally used in science and medicine.” It sounds great, but the key word here is…

Read More

Examining Cell Interactions with Surface Plasmon Resonance (SPR) and Identifying Epitopes using SPR-Mass Spectrometry (MS)

Surface plasmon resonance (SPR) offers highly efficient, label-free detection for quantifying biomolecular interactions in real-time. Two exciting SPR variants that have sprung up in recent years are SPR for cellular analysis and SPR-mass spectrometry (SPR-MS). SPR for cellular analysis allows you to study how cells attach to different substrates and each other, while SPR-mass spectrometry…

Read More

All Charged Up: The Basics of Ion-Exchange Chromatography

Highly pure proteins are vital for successful experiments; they play roles in research as assay reagents (especially for SPR applications), therapeutic candidates, and of course, as the subjects of structural and biochemical studies.  Chromatography is the science of separation and we utilize it to isolate and purify proteins based on their unique physiochemical properties. One…

Read More

The Importance of Metabolomics Research

Metabolomics may sound like a fictional character in the famous comic series “Asterix”, but it is very important in understanding systems biology and in clinical research against various diseases. Metabolomics is the last piece of a puzzle of omics applications following genomics, transcriptomics, and proteomics. It allows us to ask “What has happened and what…

Read More

Critical Factors for Full Characterization of Biosimilars

Today, about half of all therapeutic drug approvals by the US Food and Drug Administration (FDA) are for biological drugs. That number is expected to rise to 75 percent by 2025. When these biologics come off patent, the gates will open for a flood of biosimilar drugs (biopharmaceutical generics) that are designed to be much…

Read More

Key Analytical Challenges for Antibody Drug Conjugates

Currently, there are more than 75 antibody drug conjugates (ADCs) in various stages of pre-clinical and clinical development. The combination of a targeted antibody coupled with a cytotoxic small-molecule drug (via  a flexible linker) makes for a lethal and specific oncologic drug product. However, an ADC is a heterogeneous cocktail of molecules with a range…

Read More

Pack a Chromatography Column Like a Pro

You already learned the basics of column packing. When moving to more automated system using low pressure liquid chromatography systems, you can use pre-packed columns. But in order to compare several resins in specific conditions, and also to save money, you might need to pack your own low pressure columns. The art of packing a column…

Read More

To Run or to Fly: A Comparison Between HPLC and GC

Many chemical compounds, including drugs and metabolites, can be analyzed by either gas chromatography (GC) or high-performance liquid chromatography (HPLC). Because both techniques function under the same basic principles of compound separation, identification, and quantification, it can sometimes be tricky to choose one over the other. But knowing what differentiates HPLC and GC can definitely…

Read More

Ten Ways to Give Your Surface Plasmon Resonance Experiments a Hand

Surface Plasmon Resonance (SPR) is the gold standard for measuring biomolecular binding without the need for labeling (i.e., label free detection of kinetics). SPR is especially valuable because it doesn’t just provide information at the start and end of a binding event, but can be used to follow association and dissociation kinetics of biomolecules in real-time.…

Read More

How to Clean and Unclog Your HPLC Column

In my last article, I discussed how to best keep your lab’s HPLC running smoothly. However, even the best-maintained HPLCs and columns need periodic cleaning. Today, I’ll describe how to identify and troubleshoot a clogged HPLC column. Columns Are Finite First of all, it’s important to realize that columns do have a finite lifetime. The…

Read More

How to Separate Nucleotides Using Ion-paired Reverse Phase HPLC

If you work in the field of molecular biology, there is hardly a day that goes by that you don’t use nucleotides. But beyond the use of the four well-known deoxynucleotides in PCR, you can use nucleotides for several other applications. For example, kinases and phosphatases use nucleotides as substrates, and phosphotransferases transfer phosphate group…

Read More

Under Pressure: Tips for Keeping Your HPLC Up and Running Properly

If you’re anything like me, your biggest lab fear is working with expensive equipment prone to damage. HPLC is a wonderful tool, capable of separating, identifying, and quantifying a vast array of compounds, but it requires an attentive scientist to properly handle and maintain each component. In this article I’ll describe a few basic handling…

Read More

Using a Gel Filtration Chromatogram to Estimate Molecular Weight

Gel filtration chromatography (also known as size exclusion chromatography, molecular sieve chromatography, or gel permeation chromatography) is based on the differential distribution of the components in a sample between the mobile and stationary phases. Specifically, in gel filtration chromatography, this differential distribution depends on the size and shape of the components. Here, we take a…

Read More

FTIR Spectroscopy: Every Window Needs a Good Acid Wash

Fourier Transform Infrared spectroscopy (FTIR spectroscopy) is a useful and exquisitely sensitive technique used to identify and quantify unknown compounds, as well as study fine molecular details. However, to obtain a meaningful IR spectrum, it is not only important to prepare the sample correctly but also to learn how to clean the apparatus that houses…

Read More

Gel Filtration Chromatography Media: What You Need to Know

Do you use pre-packed Superdex 75 or Superdex 200 columns for gel filtration chromatography? Ever wonder if other media could better address your purification needs? Look no further. Your quest ends here! But if you’re looking for information on gel filtration principles, check out this earlier article. Choosing a Column for your Gel Filtration Experiment…

Read More

How Separation Works in Column Chromatography Methods

Separation in column chromatography relies on differences. Molecules vary in size, charge, polarity, and solubility. We leverage these differences to distribute molecules between a stationary phase and a mobile phase. But because molecules are so different, it’s not possible to have a single method that works for all. In my previous article I discussed the…

Read More

Fine-Tune Your MALDI-TOF to Produce Good-looking Mass Spectra

Mass Spec is all about getting the perfect peaks. Without a good peak assigning the correct mass is impossible and you cannot make accurate identifications. Make sure you know how to adjust your MALDI-TOF instrument parameters to achieve your perfect peak. In our previous posts ‘How does Mass Spec Work’ and ‘Imaging Mass Spectrometry: the…

Read More

The Basics of Running a Chromatography Column

Column chromatography is a common technique used to separate individual compounds from a mixture. You can use column chromatography on both a small or large scale to isolate and purify material for use on a later experiment. If you are new to column chromatography or just want a refresher, then read on for a quick…

Read More

Troubleshooting Thin Layer Chromatography: Some TLC for Your TLC

The whole TLC technique sounds easy to do, but it can be difficult and tricky during interpretation or give unexpected results, especially when working with biomolecules. For this reason, it is important to be familiar with troubleshooting thin layer chromatography. Some of the common problems faced during TLC and their solutions are listed below: Solvent…

Read More

How to Choose Your MALDI (Soul) Matrix

gif by Kronin Okay not talking about that kind of matrix. But the kind of matrix that is crucial for MALDI (Matrix-Assisted Laser Desorption Ionization). I talked briefly about MALDI in my last article, Imaging Mass Spectrometry: The New(ish) Kid on the Block. Now I want to tell you more about MALDI, specifically the matrix.…

Read More

How to Measure Molecular Weight with Gel Permeation Chromatography (GPC)

So you have a new polymer, unknown protein, or material combination but don’t know its molecular weight? An easy way to measure molecular weight of your sample is gel permeation chromatography (GPC). GPC is an analytical technique that separates molecules in polymers by size and provides the molecular weight distribution of a material. GPC is…

Read More

Thin Layer Chromatography: The Basics

Like most other chromatographic techniques, thin layer chromatography (TLC) separates out individual compounds from a mixture depending upon the polarity of each compound. The solvent system travels up a silica plate by capillary action and passes over the sample that you spot onto the plate. As the solvent travels up, it moves the compounds present…

Read More

The Many Uses of Thin Layer Chromatography

While working as biologists, we often come across mixtures of compounds, and the first question that strikes our minds is ‘what are the components in this mixture?’ One might think of using chemical assays to find the presence of specific compounds. But that sounds painful, doesn’t it? Well, the good news is that thin layer…

Read More

Carrying You Through Gas Chromatography

Maybe you found a paper in which they used gas chromatography (GC) to analyze a sample that is similar to yours and now you are wondering if you should try it too. If you are hesitant because you know little about GC, then this article will help you with that. You may already know that…

Read More

What Can NMR Do For You – Part Three

Welcome to part three of “What Can NMR Do For You” This is an ongoing series in which we describe how you can use NMR experiments to characterize your protein of interest. In part one, we explained some critical points when doing one-dimensional (1D) NMR experiments on proteins and nucleic acids (e.g. sample preparation and…

Read More

Getting the Most Out of Your Column: Optimizing Your HPLC Gradient

Let’s imagine the following scenario: You are researching a biosynthetic pathway in your favorite fungus. You know that this pathway produces a family of toxic compounds, and you want to see if you can block this pathway (or parts of it) with an antifungal drug. You have a control (no antifungal) and samples that have…

Read More

How You Can Use HPLC In Your Research

If you’re an HPLC guru, then you probably think that everyone should be using HPLC.  And you might have a point – HPLC is very powerful and has broad applications across many fields.  But it isn’t the answer to every problem. HPLC (high-performance liquid chromatography) is used to separate mixtures of compounds based on their…

Read More

The Seven Deadly Sins of HPLC

If you work with an HPLC, then you know the frustration of going to use the machine and finding it in disarray. If you’re new to using an HPLC, then the machine can be intimidating to use and you might not know the ins and outs of using it. Here’s an article that has a…

Read More

Running You Through HPLC: How Does it Work?

Your advisor tells you that he wants you to use HPLC to analyze your compound. You know that you’ve heard of this technique before, but you can’t remember what HPLC stands for, let alone how to go about doing it! We’ve all been there, though. Fear not! In this article, we will remind you about…

Read More
Scroll To Top