Haaris Safdari

Haaris Safdari
Haaris gained a Master of Science – MS Research (Molecular Biophysics – cryo-EM) from the Molecular Biophysics Unit, Indian Institute of Science (IISc), Bengaluru, India.

Articles by Haaris Safdari

Spinning Around: Tips and Tricks for Using Centrifugal Filters

Spinning Around: Tips and Tricks for Using Centrifugal Filters

One of the most widespread protein laboratory accessories are the MWCO (molecular weight cut-off) centrifugal filters which are commonly used for concentrating protein, as well as DNA. They are available commercially with different cut-offs including 3kDa, 30kDa, 50kDa, 100kDa, and so on. These little devices are expensive and hence demand proper usage and care to…

8 Tricks to Improve Your Negative Staining of Membrane Proteins

8 Tricks to Improve Your Negative Staining of Membrane Proteins

Negative staining of proteins is a versatile tool for structural biology. The sample preparation protocol is simple: the sample is embedded in a heavy metal stain that gives rise to increased specimen contrast. Thus, negative staining is a very convenient method to assess sample homogeneity, formation of macromolecular complexes, or quality of protein preparation. Conventional…