Alexandra Cuaycal

Alexandra gained a PhD in Microbiology and Cell Science from the University of Florida.

Articles by Alexandra Cuaycal

Bacterial Transformation Troubleshooting for Beginners

Bacterial Transformation Troubleshooting for Beginners

The first time I did a transformation was when I worked with site directed mutagenesis. I cloned a protein sequence into the p15TVL vector, created my mutants (but that’s another story), and was finally ready for the next step: transformation and expression of my desired protein. Little did I know that my enthusiasm would fall…