Microscopy and Imaging
A Better Alternative to Immortalized Cells: Combining CRISPR and iPSCs
Investigating human diseases and genetic variation is complex, but CRISPR-edited induced pluripotent stem cells present a promising alternative to immortalized cell lines. This article delves into genome editing principles and offers practical steps for optimizing research techniques, ensuring more accurate and ethical studies.
Read MoreConfocal Laser Scanning Microscopy Explained In 3 Easy Steps
Learn how confocal laser scanning microscopy works, its applications, and why it’s great for samples that are too thin to section.
Read MoreWhat Reagents Can You Use Past Their Chemical Expiry Date?
Learn about what reagents are usable past their chemical expiry date, how can you check if they are still okay, and which ones you should throw out.
Read MoreHow to Totally Nail Your First in situ Hybridization
Having problems with your in situ hybridization? We’ve got 7 simple tips to help you get outstanding results.
Read More5 Controls for Immunofluorescence: A Beginner’s Guide
Achieving publication-quality immunofluorescence images is tricky. Learn what controls for immunofluorescence you can use to get them!
Read MoreThe 2 Types of Digital Images: Tools to Prepare Stunning Images for Publication
Digital images are essential to communicate your data. Get the information and tools you need to take your scientific illustration to the next level!
Read MoreProtein Colocalization: 2 Essential Methods to Prove Protein Overlap
You know the drill. To prove your theory, you must show the colocalization of X and Y in a cell. Here are 2 ways to reveal protein colocalization.
Read MoreFluorescence Microscopy: From Principles to Applications
Whether you want to get started with fluorescence microscopy or already use it, this guide will ensure you know the basics and get the best out of your fluorescence microscopy.
Read MoreHow FRET Works: A Guide to Visualizing Protein Interactions
Not sure what FRET is, or just need a refresher on how FRET works? Read our short guide to understand the usefulness of FRET for studying protein-protein interactions.
Read MoreHow Using Oil Immersion Microscopy Can Increase Your Resolution
Oil immersion microscopy can improve your resolution in microscopy. This article will explain why this is the case and how you can use oil immersion microscopy in the lab!
Read MoreWhat Is Cryo-Electron Microscopy? A Brief Introduction
You don’t have to be a genius to understand Cryo-EM. Discover the fundamentals of this powerful microscopy tool and what propelled it into the scientific mainstream.
Read MoreA Short History of Cryo-Electron Microscopy
The slow, inching progress of cryo-EM towards the scientific mainstream can be told as a story with three parts. So take a step back and enjoy a short history of cryo-electron microscopy.
Read MoreCryo-EM Sample Prep: 5 Crucial Considerations
You don’t have to be a brainbox to get your samples ready for cryo-EM, but a little wisdom goes a long way. Learn how to tend to your tissues, organize your organelles, and prepare your proteins to get the micrographs you’ve always dreamed of.
Read MoreThe 2 Main Electron Microscopy Techniques: SEM vs TEM
Microscopy is a huge and active field. Sometimes, it’s easy to forget the basics. Read our biologists’ guide to electron microscopy techniques.
Read MoreApplications of Electron Microscopy: An Easy Intro for Biologists
Microscopy is a huge and active field. Sometimes, it’s easy to forget the basics. Read our biologists’ intro to applications of electron microscopy.
Read MoreScanning Electron Microscopy: 6 SEM Sample Preparation Pointers for Successful Imaging
Discover 6 critical scanning electron microscopy sample preparation points you need to know to get the best out of your SEM.
Read MoreLight-up RNA Aptamers: Illuminating the World of RNA
Discover how you can visualize that notoriously difficult molecule, RNA using light-up RNA aptamers (LURAs).
Read MoreTissue Processing For Histology: What Exactly Happens?
Tissue processing for histology is a key step between fixation and embedding. We take you through the steps of tissue processing in this simple guide.
Read MoreThe Ultimate Guide to Choosing a Fluorescent Protein
Discover the critical considerations when choosing a fluorescent protein, the key features of those most commonly used, and why newer might be better.
Read MoreAn Introduction To Fixation For Histology: Think Before You Fix!
How you fix your tissue or cells can affect your results, for better or for worse. Discover the key points to think about before undertaking your histology fixation.
Read MoreA (very) Short History of Histology
Discover the history of histology, from the first mention of a cell in 1665 to the identification and development of various stains.
Read MoreAvoid These Pitfalls: Seven (Not So Deadly) Histology Sins
Discover seven common histology mistakes and how you can avoid making them when performing your experiments.
Read MoreThat Other Number – The Meaning Of Numerical Aperture In Microscopy
Do you know what that NA number is on your objective? We walk you through what the numerical aperture is and why it’s important.
Read MoreAn Objective View: What do These Abbreviations Mean on Microscope Objectives?
There are a large number of microscope objective abbreviations relating to optical aberrations; here we’ll shed some light on some of the most common ones to get you up to speed in no time!
Read MoreLive Imaging of Phagocytosis: Your Simple Guide to Capturing the Action
Live imaging of phagocytosis helps capture the details of this dynamic process. Discover tips and tricks to visualizing this important cellular process.
Read MoreA Beginner’s Guide to The Point Spread Function
Learn how the Point Spread Function affects what you see through your microscope and discover what you can do to improve your images.
Read MoreOptimal Conditions for Live-Cell Imaging
Live-cell imaging can bring a lot of clarity to cellular processes, but keeping your cells happy can be tricky. Read on to learn about 4 key parameters for achieving optimal conditions for live-cell imaging.
Read MoreAn Introduction to Live Cell Imaging
Read on to learn more about live cell imaging, including how high rate microscopy can help you capture rapid cellular processes.
Read MoreHow History Shaped Modern Optical Microscopes, Part Two: Corrected Lenses and Objectives
Discover how chromatic and geometric imaging aberrations have been corrected over the last few centuries with the development of corrected lenses and objectives.
Read MoreHow History Shaped Modern Optical Microscopes, Part One: Simple and Compound Microscopes
Discover the history of simple and compound microscopes in this first of our two-part series on the history of microscopes.
Read MoreAdvanced Sectioning Techniques: How to Section Difficult Tissues
Are you having problems with tissue sectioning? Follow these 10 tissue sectioning tips to create the perfect tissue section every time without stressing out.
Read MoreStereo (Dissecting) Microscopes 101
Want to know about stereo microscopes? This article answers what a stereo microscope is, how it works and why it’s a great tool for biologists!
Read MoreConfocal Images: Expert Tips for Your Hour of Need!
Getting publication perfect confocal images can be tricky. If you are struggling or just want to ensure you’re capturing the best images possible, check out our top 7 tips for confocal imaging.
Read MoreResolution and Refractive Index: Set-up Your Confocal Wisely!
Get the best out of your time on the microscope by understanding the refractive index of your experiment to optimize and increase your resolution.
Read MoreFun With FRAP! Fluorescence Recovery After Photobleaching for Confocal Microscopy
If you’re thinking FRAP is short for frappuccino then you need to read this article. Discover the history, how it works, and why you’d want it in your confocal toolbox
Read MoreAn introduction to Photoactivated Localization Microscopy (PALM)
How does photoactivated localization microscopy (PALM) work? And what use can PALM microscopy be to you? This short introduction to PALM gives you the answers!
Read MoreBeginners Guide to PALM Sample Preparation
Good PALM sample preparation is the key to great images. Find out how to choose the right fluorescent proteins and learn some tips and tricks for sample prep.
Read MoreTurn a Smartphone into a Digital Microscope for Only 3 Cents
Want to build your own microscope for almost nothing? You probably already have most of the tools that you need right in your lab or at home! Here’s how.
Read MoreTrapping Objects With Light: From Star Wars to Neurons
Can lasers be used to trap and move objects? Sure they can! Read on to know how you can use lasers in a cool technique called optical tweezers to manipulate minuscule objects under the microscope.
Read MoreHow Histology Slides are Prepared
Ever wondered what magic happens to turn your samples into histology slides? Find out the 5 simple steps for histology slide preparation.
Read More