Microscopy and Imaging
Ask Eric Anything
A live episode of The Microscopists featuring Eric Betzig
Ask Eric Anything A live episode of The Microscopists featuring Eric Betzig Date: Thursday January 14, 2021Time: 4pm (London) 5pm (Berlin) 8am (Los Angeles) 11am (New York) Ask Eric Anything: A live episode of The Microscopists featuring Eric Betzig Date: Thursday January 14, 2021 Time: 4pm (London) 5pm (Berlin) 8am (Los Angeles) 11am (New York) The Microscopists…
Read More An introduction to Photoactivated Localization Microscopy (PALM)
How does photoactivated localization microscopy (PALM) work? And what use can PALM microscopy be to you? This short introduction to PALM gives you the answers!
Read More Beginners Guide to PALM Sample Preparation
Good PALM sample preparation is the key to great images. Find out how to choose the right fluorescent proteins and learn some tips and tricks for sample prep.
Read More Turn a Smartphone into a Digital Microscope for Only 3 Cents
Want to build your own microscope for almost nothing? You probably already have most of the tools that you need right in your lab or at home! If you have a smartphone, then you already have the camera for your new microscope!
Read More Trapping Objects With Light: From Star Wars to Neurons
Can lasers be used to trap and move objects? Sure they can! Read on to know how you can use lasers in a cool technique called optical tweezers to manipulate minuscule objects under the microscope.
Read More How Histology Slides are Prepared
Ever wondered what magic happens to turn your samples into histology slides? Find out the 5 simple steps for histology slide preparation.
Read More Tissue Processing For Histology: What Exactly Happens?
When preparing a sample (or multiple samples) for histology microscopy, there are multiple steps required. We’ve covered these steps in brief in a previous article on How Histology Slides are Prepared, but this article will focus on one particular procedure that needs to take place between tissue fixation and the embedding/sectioning of paraffin blocks: tissue…
Read More The Dilemma of Live Cell Imaging – How to See Something That Does not Like Light
Don’t get overexcited but we’ve got 7 top tips to help you minimize photodamage during your fluorescent live-cell imaging experiments.
Read More How to Measure Myelin Thickness Manually (and Other Tedious Lab Tasks) and Keep Your Sanity
From Organs to Tissues to Cells: Analyzing 3D Specimens with Widefield Microscopy
From Organs to Tissues to Cells: Analyzing 3D Specimens with Widefield Microscopy On Demand Speakers Dr. Falco Krüger Advanced Workflow Specialist Widefield Life Science Research DivisionLeica Microsystems Falco did his PhD in Biology at the Plant Cell Biology chair of Prof. Karin Schumacher at the Centre for Organismal Studies (COS) in Heidelberg, Germany. For his…
Read More Revealing functional mechanisms of synaptic transmission by high-pressure freezing
Revealing functional mechanisms of synaptic transmission by high-pressure freezing On Demand Speakers Frédéric Leroux Advanced workflow specialist, Leica Microsystems Frédéric Leroux completed his Master degree in Biology in 2007 at the University of Ghent where he gained experience in biological EM sample preparation. In 2008, he moved to the physics department at the University of…
Read More Structured Illumination Microscopy (SIM) – An Introduction
Discover how structured illumination microscopy works can help you see things in greater detail.
Read More Making the Perfect Blood Smear
Stop wasting time throwing out slide after slide trying to create a readable blood smear. Read our how-to guide for creating the perfect blood smear slide.
Read More What Can Electron Microscopy Do for You?
The electron microscope (EM) – where electrons, rather than photons, make the image – fell out of fashion for a while, but it has come back refreshed. Modern electron microscopes cost less, use less electricity, and are generally easier to maintain than the older models, so it is likely that you can get your hands on one. Read on to learn more about this technique, and how to implement it into your research.
Read More Workflows and instrumentation for cryo-electron microscopy: an overview
Workflows and instrumentation for cryo-electron microscopy: an overview Now On Demand Speaker Frédéric Leroux Advanced workflow specialist, Leica Microsystems Frédéric Leroux completed his Master degree in Biology in 2007 at the University of Ghent where he gained experience in biological EM sample preparation. In 2008, he moved to the physics department at the University of…
Read More Microscope Disinfection: A Quick Guide
Shared microscopes have the potential to get dirty and spread nasties between samples and users. Check out our quick guide on how to clean and disinfect your microscope.
Read More Imaging and Analyzing Your Wound Healing Assay
Don’t be daunted by imaging and analyzing your wound healing assay; our top tips will get it looking perfect.
Read More Making a Mark: How to Set up Your Wound Healing Assay
Are you wanting to study cell migration and need a way to quantify it? A wound healing assay is one of the earliest and simplest in-vitro assays developed to study cell migration. Collective cell migration is a characteristic behaviour seen in wound repair, cancer invasion, cancer metastasis, immune response, angiogenesis and embryonic morphogenesis. Depending on…
Read More How to Totally Nail Your First in situ Hybridization
It’s Monday morning and you’re about to start your first in situ hybridization (ISH). You glanced at the protocol on Friday and it seemed pretty easy. You just do a lot of washes and incubations, right? WRONG. ISH can be a nightmare. If you’re not well prepared, you’ll most likely be tearing your hair out…
Read More Controls for Immunofluorescence: A Beginner’s Guide
Immunofluorescence staining is a popular and extremely powerful detection method. However, achieving publication quality immunofluorescence or fluorescent antibody staining can get tricky. It’s therefore important to ensure you have the right controls for immunofluorescence. Since immunofluorescence staining is a long process with many steps, it is always advisable to spend some time optimizing each…
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