Microscopy and Imaging
How Histology Slides are Prepared
Ever wondered what magic happens to turn your samples into histology slides? Find out the 5 simple steps for histology slide preparation.
Read More Tissue Processing For Histology: What Exactly Happens?
When preparing a sample (or multiple samples) for histology microscopy, there are multiple steps required. We’ve covered these steps in brief in a previous article on How Histology Slides are Prepared, but this article will focus on one particular procedure that needs to take place between tissue fixation and the embedding/sectioning of paraffin blocks: tissue…
Read More The Dilemma of Live Cell Imaging – How to See Something That Does not Like Light
Don’t get overexcited but we’ve got 7 top tips to help you minimize photodamage during your fluorescent live-cell imaging experiments.
Read More How to Measure Myelin Thickness Manually (and Other Tedious Lab Tasks) and Keep Your Sanity
From Organs to Tissues to Cells: Analyzing 3D Specimens with Widefield Microscopy
From Organs to Tissues to Cells: Analyzing 3D Specimens with Widefield Microscopy On Demand Speakers Dr. Falco Krüger Advanced Workflow Specialist Widefield Life Science Research DivisionLeica Microsystems Falco did his PhD in Biology at the Plant Cell Biology chair of Prof. Karin Schumacher at the Centre for Organismal Studies (COS) in Heidelberg, Germany. For his…
Read More Revealing functional mechanisms of synaptic transmission by high-pressure freezing
Revealing functional mechanisms of synaptic transmission by high-pressure freezing On Demand Speakers Frédéric Leroux Advanced workflow specialist, Leica Microsystems Frédéric Leroux completed his Master degree in Biology in 2007 at the University of Ghent where he gained experience in biological EM sample preparation. In 2008, he moved to the physics department at the University of…
Read More Structured Illumination Microscopy (SIM) – An Introduction
Discover how structured illumination microscopy works can help you see things in greater detail.
Read More Making the Perfect Blood Smear
Stop wasting time throwing out slide after slide trying to create a readable blood smear. Read our how-to guide for creating the perfect blood smear slide.
Read More What Can Electron Microscopy Do for You?
The electron microscope (EM) – where electrons, rather than photons, make the image – fell out of fashion for a while, but it has come back refreshed. Modern electron microscopes cost less, use less electricity, and are generally easier to maintain than the older models, so it is likely that you can get your hands on one. Read on to learn more about this technique, and how to implement it into your research.
Read More Workflows and instrumentation for cryo-electron microscopy: an overview
Workflows and instrumentation for cryo-electron microscopy: an overview Now On Demand Speaker Frédéric Leroux Advanced workflow specialist, Leica Microsystems Frédéric Leroux completed his Master degree in Biology in 2007 at the University of Ghent where he gained experience in biological EM sample preparation. In 2008, he moved to the physics department at the University of…
Read More Microscope Disinfection: A Quick Guide
Shared microscopes have the potential to get dirty and spread nasties between samples and users. Check out our quick guide on how to clean and disinfect your microscope.
Read More Imaging and Analyzing Your Wound Healing Assay
Don’t be daunted by imaging and analyzing your wound healing assay; our top tips will get it looking perfect.
Read More Making a Mark: How to Set up Your Wound Healing Assay
Are you wanting to study cell migration and need a way to quantify it? A wound healing assay is one of the earliest and simplest in-vitro assays developed to study cell migration. Collective cell migration is a characteristic behaviour seen in wound repair, cancer invasion, cancer metastasis, immune response, angiogenesis and embryonic morphogenesis. Depending on…
Read More How to Totally Nail Your First in situ Hybridization
It’s Monday morning and you’re about to start your first in situ hybridization (ISH). You glanced at the protocol on Friday and it seemed pretty easy. You just do a lot of washes and incubations, right? WRONG. ISH can be a nightmare. If you’re not well prepared, you’ll most likely be tearing your hair out…
Read More Controls for Immunofluorescence: A Beginner’s Guide
Immunofluorescence staining is a popular and extremely powerful detection method. However, achieving publication quality immunofluorescence or fluorescent antibody staining can get tricky. It’s therefore important to ensure you have the right controls for immunofluorescence. Since immunofluorescence staining is a long process with many steps, it is always advisable to spend some time optimizing each…
Read More Optimized cryo workflows using the vacuum cryo transfer system VCT500
Optimized cryo workflows using the vacuum cryo transfer system VCT500 In this tutorial, you will find: How to use the vacuum cryo transfer system The advantages of the EM VCT500 and EM VCM for different cryo workflows About their flexible integration with different analysis systems Speakers Dr. Julia KönigProduct Manager EM sample preparation for cryo-workflows…
Read More THUNDER Imagers: High performance, versatility and ease-of-use for your everyday imaging workflows.
THUNDER Imagers: High performance, versatility and ease-of-use for your everyday imaging workflows. In this webinar, you will learn: How to get sharp, high quality images from thick 3-dimensional microscope samples How to shorten your imaging workflow from image acquisition to data analysis A broad spectrum of imaging workflows for a range of life science applications…
Read More Understanding Malaria Parasite Pathogenesis by Cryo-Electron Microscopy
Understanding Malaria Parasite Pathogenesis by Cryo-Electron Microscopy In this tutorial, you will learn: The mechanisms by which the plasmodium translocon of exported proteins (PTEX) enables the Plasmodium parasite to take over red blood cells How single-particle cryo-electron microscopy enables us to gain insight into the PTEX molecular structure The opportunity this presents for structure-based design…
Read More How History Shaped Modern Optical Microscopes, Part One: Simple and Compound Microscopes
Having some knowledge of microscope history can be beneficial to understanding and appreciating specific configurations and components, and how they produce an optimal image. For example, practically all laboratory microscopes use achromatic objectives to partly compensate for chromatic aberration. For high-quality imaging, fluorescence microscopy, and photomicroscopy, fluorite and apochromatic objectives are used to largely overcome…
Read More An Introduction to Digital Images in Publications
Previously, I wrote about the different ways to use scientific illustration to communicate research. In this article, I will introduce the technical aspects of digital images and the most common image file types that scientists will encounter when preparing images for publication. Raster Versus Vector Digital images are classified based on how information is stored.…
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