Microscopy and Imaging
A Beginner’s Guide to The Point Spread Function
Learn how the Point Spread Function affects what you see through your microscope and discover what you can do to improve your images.
Read More Intravital Microscopy of Cancer
LIVE WebinarIntravital Microscopy of Cancer Tuesday 29th June 2021 LONDON 16:00 BST | BERLIN 17:00 CET | DUBAI 19:00 GST In this webinar, you will learn: about intravital microscopy techniques that allow you to monitor stem cells and cancer cells in real-time in vivo; how these techniques can help you study the identity and migratory…
Read More Optimal Conditions for Live-Cell Imaging
Live-cell imaging can bring a lot of clarity to cellular processes, but keeping your cells happy can be tricky. Read on to learn about 4 key parameters for achieving optimal conditions for live-cell imaging.
Read More An Introduction to Live Cell Imaging
Read on to learn more about live cell imaging, including how high rate microscopy can help you capture rapid cellular processes.
Read More Visualising protein degradation and aggregation in the living cell
Visualising protein degradation and aggregation in the living cell Available On Demandat MicroscopyFocus.com Watch Now In this webinar, Prof. Eric Reits will discuss how several neurodegenerative disorders, including Alzheimer’s, Parkinson’s and Huntington’s disease, are characterized by the formation of protein aggregates, and that components of the ubiquitin-proteasome system (UPS) co-localize in these aggregates. This has…
Read More Life beyond the pixels: deep learning methods for single cell analysis
Life beyond the pixels:Deep learning methods for single cell analysis Available On Demand In this webinar, you will discover: the computational steps in the analysis of single cell-based large-scale microscopy experiments; a novel microscopic image correction method designed to eliminate illumination and uneven background effects; new single-cell image segmentation methods; the Advanced Cell Classifier, a…
Read More How History Shaped Modern Optical Microscopes, Part Two: Corrected Lenses and Objectives
Discover how chromatic and geometric imaging aberrations have been corrected over the last few centuries with the development of corrected lenses and objectives.
Read More See The Hidden: Virtual Neurosciences Workshop
See The Hidden: Virtual Neurosciences Workshop This live online event brings together a series of scientific talks and microscopy showcases, centering around four specific topics in microscopy – 3D real time imaging*, laser microdissection, confocal nanoscopy and high pressure freezing techniques in electron microscopy. Each section will kick off with a scientific talk from one…
Read More How History Shaped Modern Optical Microscopes, Part One: Simple and Compound Microscopes
Discover the history of simple and compound microscopes in this first of our two-part series on the history of microscopes.
Read More Advanced Sectioning Techniques: How to Section Difficult Tissues
Are you having problems with tissue sectioning? Follow these 10 tissue sectioning tips to create the perfect tissue section every time without stressing out.
Read More Stereo (Dissecting) Microscopes 101
Want to know about stereo microscopes? This article answers what a stereo microscope is, how it works and why it’s a great tool for biologists!
Read More UltraMicroscope Blaze™ live instrument demonstration
UltraMicroscope Blaze™ live instrument demonstration Available On-Demand Register & Watch Now This live demonstration introduces you directly to the Ultramicroscope Blaze, a fully-automated, multi-scale, high-resolution, light sheet imaging system for high throughput volumetric imaging of large biological samples. Our imaging team will demonstrate the ease-of-use and performance of the instrument and take you through the…
Read More The UltraMicroscope Blaze™: Automated 3D imaging on an unprecedented scale
The UltraMicroscope Blaze™: Automated 3D imaging on an unprecedented scale Available On-Demand Register & Watch Now This webinar introduces the brand new Ultramicroscope Blaze. This next-generation light sheet imaging system brings together more than a decade of light sheet fluorescence microscopy (LSFM) development and collaboration. The Blaze is a fully-automated light sheet imaging system with,…
Read More Mesoscopic Whole-Organ Imaging: Experimental Models and Diagnostic Potential
Mesoscopic Whole-Organ Imaging: Experimental Models and Diagnostic Potential Available On-Demand Register & Watch Now Cellular interactions and thus organ function and anatomy are inherently of a three-dimensional (3D) nature. Knowledge of the cellular distribution patterns within organs, in both health and disease states, is critical to our understanding of ongoing microscopic processes on a mesoscopic…
Read More Confocal Images: Expert Tips for Your Hour of Need!
Getting publication perfect confocal images can be tricky. If you are struggling or just want to ensure you’re capturing the best images possible, check out our top 7 tips for confocal imaging.
Read More Resolution and Refractive Index: Set-up Your Confocal Wisely!
Get the best out of your time on the microscope by understanding the refractive index of your experiment to optimize and increase your resolution.
Read More Fun With FRAP! Fluorescence Recovery After Photobleaching for Confocal Microscopy
If you’re thinking FRAP is short for frappuccino then you need to read this article. Discover the history, how it works, and why you’d want it in your confocal toolbox
Read More Exploring The Structure and Life Cycle of Viruses
Exploring The Structure and Life Cycle of Viruses The Virologist’s Toolbox for Electron Microscopy Specimen Preparation Available On Demand Speakers Frédéric Leroux Advanced Workflow SpecialistLeica Microsystems Frédéric Leroux completed his Master degree in Biology in 2007 at the University of Ghent where he gained experience in biological EM sample preparation. In 2008, he moved to…
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Ask Eric Anything
A live episode of The Microscopists featuring Eric Betzig
Ask Eric Anything A live episode of The Microscopists featuring Eric Betzig On-Demand Ask Eric Anything: A live episode of The Microscopists featuring Eric Betzig On-Demand The Microscopists is going LIVE! For a one-off and very special guest – Eric Betzig! We already know so much about Eric, but I hope that our chat will…
Read More An introduction to Photoactivated Localization Microscopy (PALM)
How does photoactivated localization microscopy (PALM) work? And what use can PALM microscopy be to you? This short introduction to PALM gives you the answers!
Read More Beginners Guide to PALM Sample Preparation
Good PALM sample preparation is the key to great images. Find out how to choose the right fluorescent proteins and learn some tips and tricks for sample prep.
Read More Turn a Smartphone into a Digital Microscope for Only 3 Cents
Want to build your own microscope for almost nothing? You probably already have most of the tools that you need right in your lab or at home! If you have a smartphone, then you already have the camera for your new microscope!
Read More Trapping Objects With Light: From Star Wars to Neurons
Can lasers be used to trap and move objects? Sure they can! Read on to know how you can use lasers in a cool technique called optical tweezers to manipulate minuscule objects under the microscope.
Read More How Histology Slides are Prepared
Ever wondered what magic happens to turn your samples into histology slides? Find out the 5 simple steps for histology slide preparation.
Read More Tissue Processing For Histology: What Exactly Happens?
When preparing a sample (or multiple samples) for histology microscopy, there are multiple steps required. We’ve covered these steps in brief in a previous article on How Histology Slides are Prepared, but this article will focus on one particular procedure that needs to take place between tissue fixation and the embedding/sectioning of paraffin blocks: tissue…
Read More The Dilemma of Live Cell Imaging – How to See Something That Does not Like Light
Don’t get overexcited but we’ve got 7 top tips to help you minimize photodamage during your fluorescent live-cell imaging experiments.
Read More How to Measure Myelin Thickness Manually (and Other Tedious Lab Tasks) and Keep Your Sanity
From Organs to Tissues to Cells: Analyzing 3D Specimens with Widefield Microscopy
From Organs to Tissues to Cells: Analyzing 3D Specimens with Widefield Microscopy On Demand Speakers Dr. Falco Krüger Advanced Workflow Specialist Widefield Life Science Research DivisionLeica Microsystems Falco did his PhD in Biology at the Plant Cell Biology chair of Prof. Karin Schumacher at the Centre for Organismal Studies (COS) in Heidelberg, Germany. For his…
Read More Revealing functional mechanisms of synaptic transmission by high-pressure freezing
Revealing functional mechanisms of synaptic transmission by high-pressure freezing On Demand Speakers Frédéric Leroux Advanced workflow specialist, Leica Microsystems Frédéric Leroux completed his Master degree in Biology in 2007 at the University of Ghent where he gained experience in biological EM sample preparation. In 2008, he moved to the physics department at the University of…
Read More Structured Illumination Microscopy (SIM) – An Introduction
Discover how structured illumination microscopy works can help you see things in greater detail.
Read More Making the Perfect Blood Smear
Stop wasting time throwing out slide after slide trying to create a readable blood smear. Read our how-to guide for creating the perfect blood smear slide.
Read More What Can Electron Microscopy Do for You?
The electron microscope (EM) – where electrons, rather than photons, make the image – fell out of fashion for a while, but it has come back refreshed. Modern electron microscopes cost less, use less electricity, and are generally easier to maintain than the older models, so it is likely that you can get your hands on one. Read on to learn more about this technique, and how to implement it into your research.
Read More Workflows and instrumentation for cryo-electron microscopy: an overview
Workflows and instrumentation for cryo-electron microscopy: an overview Now On Demand Speaker Frédéric Leroux Advanced workflow specialist, Leica Microsystems Frédéric Leroux completed his Master degree in Biology in 2007 at the University of Ghent where he gained experience in biological EM sample preparation. In 2008, he moved to the physics department at the University of…
Read More Microscope Disinfection: A Quick Guide
Shared microscopes have the potential to get dirty and spread nasties between samples and users. Check out our quick guide on how to clean and disinfect your microscope.
Read More Imaging and Analyzing Your Wound Healing Assay
Don’t be daunted by imaging and analyzing your wound healing assay; our top tips will get it looking perfect.
Read More Making a Mark: How to Set up Your Wound Healing Assay
Are you wanting to study cell migration and need a way to quantify it? A wound healing assay is one of the earliest and simplest in-vitro assays developed to study cell migration. Collective cell migration is a characteristic behaviour seen in wound repair, cancer invasion, cancer metastasis, immune response, angiogenesis and embryonic morphogenesis. Depending on…
Read More How to Totally Nail Your First in situ Hybridization
It’s Monday morning and you’re about to start your first in situ hybridization (ISH). You glanced at the protocol on Friday and it seemed pretty easy. You just do a lot of washes and incubations, right? WRONG. ISH can be a nightmare. If you’re not well prepared, you’ll most likely be tearing your hair out…
Read More Controls for Immunofluorescence: A Beginner’s Guide
Immunofluorescence staining is a popular and extremely powerful detection method. However, achieving publication quality immunofluorescence or fluorescent antibody staining can get tricky. It’s therefore important to ensure you have the right controls for immunofluorescence. Since immunofluorescence staining is a long process with many steps, it is always advisable to spend some time optimizing each…
Read More Optimized cryo workflows using the vacuum cryo transfer system VCT500
Optimized cryo workflows using the vacuum cryo transfer system VCT500 In this tutorial, you will find: How to use the vacuum cryo transfer system The advantages of the EM VCT500 and EM VCM for different cryo workflows About their flexible integration with different analysis systems Speakers Dr. Julia KönigProduct Manager EM sample preparation for cryo-workflows…
Read More THUNDER Imagers: High performance, versatility and ease-of-use for your everyday imaging workflows.
THUNDER Imagers: High performance, versatility and ease-of-use for your everyday imaging workflows. In this webinar, you will learn: How to get sharp, high quality images from thick 3-dimensional microscope samples How to shorten your imaging workflow from image acquisition to data analysis A broad spectrum of imaging workflows for a range of life science applications…
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