Microscopy and Imaging
Revealing functional mechanisms of synaptic transmission by high-pressure freezing
Revealing functional mechanisms of synaptic transmission by high-pressure freezing Date: Thursday October 15th 2020Time: 1 pm (London) 2 pm (Berlin) Speakers Frédéric Leroux Advanced workflow specialist, Leica Microsystems Frédéric Leroux completed his Master degree in Biology in 2007 at the University of Ghent where he gained experience in biological EM sample preparation. In 2008, he…
Read More Making the Perfect Blood Smear
Stop wasting time throwing out slide after slide trying to create a readable blood smear. Read our how-to guide for creating the perfect blood smear slide.
Read More What Can Electron Microscopy Do for You?
The electron microscope (EM) – where electrons, rather than photons, make the image – fell out of fashion for a while, but it has come back refreshed. Modern electron microscopes cost less, use less electricity, and are generally easier to maintain than the older models, so it is likely that you can get your hands on one. Read on to learn more about this technique, and how to implement it into your research.
Read More Workflows and instrumentation for cryo-electron microscopy: an overview
Workflows and instrumentation for cryo-electron microscopy: an overview Now On Demand Speaker Frédéric Leroux Advanced workflow specialist, Leica Microsystems Frédéric Leroux completed his Master degree in Biology in 2007 at the University of Ghent where he gained experience in biological EM sample preparation. In 2008, he moved to the physics department at the University of…
Read More Microscope Disinfection: A Quick Guide
Shared microscopes have the potential to get dirty and spread nasties between samples and users. Check out our quick guide on how to clean and disinfect your microscope.
Read More Imaging and Analyzing Your Wound Healing Assay
Don’t be daunted by imaging and analyzing your wound healing assay; our top tips will get it looking perfect.
Read More Making a Mark: How to Set up Your Wound Healing Assay
Are you wanting to study cell migration and need a way to quantify it? A wound healing assay is one of the earliest and simplest in-vitro assays developed to study cell migration. Collective cell migration is a characteristic behaviour seen in wound repair, cancer invasion, cancer metastasis, immune response, angiogenesis and embryonic morphogenesis. Depending on…
Read More How to Totally Nail Your First in situ Hybridization
It’s Monday morning and you’re about to start your first in situ hybridization (ISH). You glanced at the protocol on Friday and it seemed pretty easy. You just do a lot of washes and incubations, right? WRONG. ISH can be a nightmare. If you’re not well prepared, you’ll most likely be tearing your hair out…
Read More Controls for Immunofluorescence: A Beginner’s Guide
Immunofluorescence staining is a popular and extremely powerful detection method. However, achieving publication quality immunofluorescence or fluorescent antibody staining can get tricky. It’s therefore important to ensure you have the right controls for immunofluorescence. Since immunofluorescence staining is a long process with many steps, it is always advisable to spend some time optimizing each…
Read More Optimized cryo workflows using the vacuum cryo transfer system VCT500
Optimized cryo workflows using the vacuum cryo transfer system VCT500 In this tutorial, you will find: How to use the vacuum cryo transfer system The advantages of the EM VCT500 and EM VCM for different cryo workflows About their flexible integration with different analysis systems Speakers Dr. Julia KönigProduct Manager EM sample preparation for cryo-workflows…
Read More THUNDER Imagers: High performance, versatility and ease-of-use for your everyday imaging workflows.
THUNDER Imagers: High performance, versatility and ease-of-use for your everyday imaging workflows. In this webinar, you will learn: How to get sharp, high quality images from thick 3-dimensional microscope samples How to shorten your imaging workflow from image acquisition to data analysis A broad spectrum of imaging workflows for a range of life science applications…
Read More Understanding Malaria Parasite Pathogenesis by Cryo-Electron Microscopy
Understanding Malaria Parasite Pathogenesis by Cryo-Electron Microscopy In this tutorial, you will learn: The mechanisms by which the plasmodium translocon of exported proteins (PTEX) enables the Plasmodium parasite to take over red blood cells How single-particle cryo-electron microscopy enables us to gain insight into the PTEX molecular structure The opportunity this presents for structure-based design…
Read More How History Shaped Modern Optical Microscopes, Part One: Simple and Compound Microscopes
Having some knowledge of microscope history can be beneficial to understanding and appreciating specific configurations and components, and how they produce an optimal image. For example, practically all laboratory microscopes use achromatic objectives to partly compensate for chromatic aberration. For high-quality imaging, fluorescence microscopy, and photomicroscopy, fluorite and apochromatic objectives are used to largely overcome…
Read More An Introduction to Digital Images in Publications
Previously, I wrote about the different ways to use scientific illustration to communicate research. In this article, I will introduce the technical aspects of digital images and the most common image file types that scientists will encounter when preparing images for publication. Raster Versus Vector Digital images are classified based on how information is stored.…
Read More Basics of Fluorescence Lifetime Imaging and FLIM Applications to FRET Biosensors
Basics of Fluorescence Lifetime Imaging and FLIM Applications to FRET Biosensors In this tutorial you will learn: How researchers (Anzilotti 2019) used FLIM to elucidate the mechanisms responsible for a B cell deficiency A new approach to measure the cellular function of the Zn2+ transporter ZIP7 in primary mouse cells, comparing wild-type and CRISPR-Cas9 derived mutant cells…
Read More How to Weigh Molecules with Light Using Mass Photometry
How to Weigh Molecules with Light Using Mass Photometry In this tutorial, you will learn: How mass photometry offers a universal way to study biomolecules The strengths and limitations of mass photometry How to use mass photometry to: accurately determine the degree of heterogeneity of a sample quantify the strength and kinetics of molecular interactions…
Read More The New ZEISS LSM 9 Family: Adding the Multiplex Factor to Your Confocal Imaging
The New ZEISS LSM 9 Family: Adding the Multiplex Factor to Your Confocal Imaging In this tutorial on confocal imaging, you will learn how you can: Capture weaker signals—and still get sound, reproducible data. Reach faster volumetric imaging without sacrificing resolution Increase data throughput for your imaging needs Speaker Joseph HuffSolution Manager/Application Development Engineer at…
Read MoreCryofixation and Chemical Fixation for Electron Microscopy
Specimen preparation is the most important aspect of biological electron microscopy (EM), as it influences everything from the preservation of the sample itself to the kind of information that can be obtained. It is vital to define the questions you are asking of the sample before embarking on an electron microscopy project. This enables you…
Read More Ultramicrotomy for Electron Microscopy
Ultramicrotomy is the process by which a sample is cut into very thin slices or “sections”, usually for imaging by transmission electron microscopy (TEM) or relatively new techniques using scanning electron microscopy (See Array tomography in three dimensional scanning electron microscopy for biology). This technique requires a bit of finesse, and this article will help…
Read More Microscope Finder Slides: Their History, Development, and Use
Have you ever been looking through a box of slides and found something that you want to image or look at later, or even show to one of your colleagues or supervisor? Finding that exact spot on the slide at a later date can prove to be difficult- using a marker pen on the coverslip…
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