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While CRISPR offers vast applications in disease research and drug target identification, it’s not always the optimal choice for every scenario. Explore the main advantages and challenges of using CRISPR-Cas9 to determine if it’s the right fit for your project.
Want to do some epigenome editing? Discover the usefulness of catalytically inactive (dead) Cas9.
Level-up your troubleshooting ability by determining the success of failure of each stage of your CRISPR experiment.
Allele-specific expression can occur for various biological reasons, such as gene imprinting, or differential transcription caused by mutations, or single nucleotide polymorphisms (SNPs), or epigenetic alterations. Traditional end-point RT-PCR or qRT-PCR-based methods only detect overall levels of mRNA expression from a given gene rather than mRNA transcripts originating from individuals. If your project requires more…
The cell cycle has been very well documented over the years because of its dysregulation in diseases such as cancer. Many different processes contribute to cell growth and replication, which is ultimately controlled by a series of tightly controlled cell cycle phases. For some areas of research, especially within drug discovery and cancer research, cell synchronization in…
Thousands upon thousands of genetic variants are now associated with every disease and trait you can possibly think of. Such traits range from cancers to blood pressure, intelligence, height, weight… and many more! This is largely because of the advent of genome-wide association studies (GWAS). However, the vast majority of genetic loci associated with these traits are…
Part of the fun of science is the opportunity to conjure up hypotheses and ways in which to test them. Of course, being scientists, our ideas are highly thought out and logical. We aim to test the hypothesis which we think is the most likely explanation using the available supporting evidence. In addition, it is…
So, the genome-wide association study (GWAS) data for your disease of interest was published, and it has thrown up some very interesting associations. However, at this stage, bear in mind that this is only an association. Your project is to provide the link between the GWAS single nucleotide polymorphisms (SNP) and pathological changes. Where do…
For many students, a PhD project is the first opportunity to really sink your teeth into your very own research project over a long period of time. This initial period is exciting but can also be a little daunting. Where do you begin? How do you actually design your first experiments? I mean, what are…
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