Codon Optimization for Increased Protein Expression
The Genetic Code: A Universal Template for Protein Translation All known organisms share the ‘central dogma’ of molecular biology. DNA is transcribed into mRNA that is translated into protein. During the discovery of the genetic code, Francis Crick hypothesized that translation required a mediator to aid mRNA-guided translation according to a number of specifics. Amongst…
Antibody Validation
Not all antibodies are valid for every experiment and condition, and they must be validated for the specific application and species. Currently, there is no standard means of “antibody validation,” and this can greatly impact experimental reproducibility and reliability. Journals and granting agencies have taken steps to address this gap. Many now require you to…
How to Design a CRISPR Cas9 Experiment and Start Genome Editing
Designing a CRISPR experiment can be daunting. We’ve got tips and pointers to help you get off on the right foot.