-
How to Use CRISPR to Accelerate Cancer Therapies
How to Use CRISPR to Accelerate Cancer Therapies Join Theo Roth as he describes his lab’s novel CRISPR-Cas9 genome-targeting system that does not require viral vectors to modify T cell genomes, but instead focuses on HDR. This allows rapid and efficient insertion of large DNA sequences at specific sites in the genomes of primary human…
-
A Guide to Harnessing DNA Assembly for Drug Discovery
Current DNA synthesis and assembly technologies give today’s genetic engineers unprecedented freedom to control every aspect of genetic design. In this webinar on DNA assembly, you will learn: Key concepts in DNA assembly The importance of analyzing combinatorial libraries of genetic designs for natural product biosynthesis Emerging areas of research Join Dr. Michael Smanski as…
-
Understanding and Designing Flanking Homology DNA Assembly Experiments
Understanding and Designing Flanking Homology DNA Assembly Experiments Join us in this webinar on demystifying DNA assembly. In this tutorial, you will find: How flanking homology DNA assembly methods work How to use web-based software to design experimental methods for flanking homology DNA assembly methods How synthetic DNA fragments fit in to the DNA assembly…
-
Codon Optimization for Increased Protein Expression
The Genetic Code: A Universal Template for Protein Translation All known organisms share the ‘central dogma’ of molecular biology. DNA is transcribed into mRNA that is translated into protein. During the discovery of the genetic code, Francis Crick hypothesized that translation required a mediator to aid mRNA-guided translation according to a number of specifics. Amongst…
-
Antibody Validation
Not all antibodies are valid for every experiment and condition, and they must be validated for the specific application and species. Currently, there is no standard means of “antibody validation,” and this can greatly impact experimental reproducibility and reliability. Journals and granting agencies have taken steps to address this gap. Many now require you to…
-
How to Design a CRISPR Experiment and Start Genome Editing
CRISPR and the CRISPR Associated system (Cas) are powerful gene editing technologies. Originally identified and characterized in bacteria, the endogenous CRISPR systems act as an RNA-based defense mechanism against invading phage DNA. CRISPR cas9 gene editing was adapted for genome editing in 2013 and has since been exploited for its ability to generate targeted double-stranded DNA…
-
Engineering Cell Behavior with Synthetic Biology
Engineering Cell Behavior with Synthetic Biology In this webinar, you will learn how synthetic biology can be used to engineer desirable and robust behaviors or traits into model organisms. The main aspects to be covered in this webinar include: Introduction to synthetic biology – what it is and what it’s not Popular applications for synthetic…
-
Development of a Potential Recombinant Protein Vaccine in E. Coli
Expression and Purification of an Engineered, E. coli-expressed Leishmania donovani Nucleoside Hydrolase with Immunogenic Properties Potential recombinant protein vaccine candidates must meet several criteria: They must be expressed at sufficiently high levels in the organism of choice They must be purified to high purity from the expression system in an immunogenic form They must induce…
-
Guide to CRISPR/Cas9 Delivery: How to Maximize Your Editing Efficiency
In this webinar, you will learn how to maximize your genome editing efficiency using CRISPR/Cas9 and how to apply this technique in your research. The main points in the webinar will include: How to design guide RNAs using online tools specific to the genome and application of interest. Tips and practical advice to assist you…
