Techniques
Maximizing Protein Extraction from Yeast Cells: A Comparison of 4 Methods
Confused about yeast protein extraction methods? Let this article guide you through the process and help you select the most appropriate method based on your research goals and available resources.
Read MoreWhat Reagents Can You Use Past Their Chemical Expiry Date?
Learn about what reagents are usable past their chemical expiry date, how can you check if they are still okay, and which ones you should throw out.
Read MoreA Beginner’s Guide to How Blunt-End Cloning Works
Struggling with your cloning? It could be time to try blunt-end cloning. This easy introduction explains what it is, when to use it, and how.
Read More7 Tips for Incredible in situ Hybridization
Having problems with your in situ hybridization? We’ve got 7 simple tips to help you get outstanding results.
Read More5 Controls for Immunofluorescence: A Beginner’s Guide
Achieving publication-quality immunofluorescence images is tricky. Learn what controls for immunofluorescence you can use to get them!
Read MoreWhat is Water of Crystallization? Everything You Need to Know
Not sure what water of crystallization is? This article explains everything you need to know so that you’re equipped with the knowledge to decide if it matters to your experiments.
Read MoreThe 2 Types of Digital Images: Tools to Prepare Stunning Images for Publication
Digital images are essential to communicate your data. Get the information and tools you need to take your scientific illustration to the next level!
Read MoreWhat Is Protein Crystallography? Structure Solution Explained
What is protein crystallography? Whether you are about to enter the world of protein crystallography or work with a protein crystallographer on your grant, this article will be useful.
Read MoreProtein Colocalization: 2 Essential Methods to Prove Protein Overlap
You know the drill. To prove your theory, you must show the colocalization of X and Y in a cell. Here are 2 ways to reveal protein colocalization.
Read MoreWhat Ponceau S Staining Can Reveal About Your Western Blot
Discover how Ponceau S works, how to perform staining, and get a detailed guide to interpreting what the staining means when troubleshooting your failed blots.
Read MoreFluorescence Microscopy: From Principles to Applications
Whether you want to get started with fluorescence microscopy or already use it, this guide will ensure you know the basics and get the best out of your fluorescence microscopy.
Read MoreHow to Build Your Own PCR Machine
You can build your own PCR machine using tools and equipment you can get easily and without breaking the bank. Well, what are you waiting for?
Read MoreHow To Make ECL Reagent: 4 Ingredients, Better Blots
ECL is an expensive reagent. Why not learn how to make ECL yourself? This cheap and simple option will give you better blots more often!
Read MoreHow To Passage Cells in Culture
Learn the basics of how to passage cells in culture with this easy-to-follow guide. While the specifics change per cell line, the basics remain.
Read More12 Top Tips for Working in a Biosafety Cabinet
Biosafety cabinets keep your work safe from contamination, and you safe from your work. So here are 12 tips for Working in a biosafety cabinet!
Read MoreNail Your Unmixing for Full Spectrum Flow Cytometry: 7 Top Tips
Spectral unmixing in flow cytometry is the key to great data from your full spectrum flow cytometry. Get this wrong, and you risk unreliable results. Read our top 7 tips from a flow cytometry core facilities expert to nail your unmixing.
Read MoreWhat’s in Your Cell Culture Medium?
Do you know what each ingredient in cell culture media does? Read on to take a look at the common ingredients and break down their roles.
Read More3 Hot Tips to Optimize Your Western Blot Transfers
Are you struggling with ugly and unreadable western blots? Here are 3 ways to optimize your western blot transfer and get blots to be proud of.
Read MoreUnderstanding Cell Passage Number and How to Calculate it for Cell Cultures
Not sure what we mean by cell passage number? Confused about how to calculate it? Wondering if there is a maximum number? We explain it all.
Read MoreHow to Measure Molecular Weight Using Size-Exclusion Chromatography (SEC)
We often use size-exclusion chromatography to purify samples. Read this article to learn how to use it to measure sample molecular weight as well!
Read MoreHPLC and GC: 6 Fundamental Differences Made Simple to Enhance Your Research
Need to get a handle on the fundamental differences between HPLC and GC to pick one for your studies? Here’s a quick explainer!
Read MoreTop 5 of the Most Commonly Used Cell Lines
Want to use a cell line but not sure where to start? Or perhaps you’re just curious about the most commonly used cell lines. Our top 5 most commonly used cell lines will help you get a feel for the cells that many researchers turn to.
Read MoreGoing Serum-Free: The How and Why of Removing Serum From Your Media
While using serum in cell culture can keep your cells healthy and happy, there are some notable downsides. We discuss the pros and cons of using serum in cell culture media and how to remove it if you want to eliminate it from your workflow.
Read MoreGas Chromatography: What It Is, How It Works, and 5 Critical Components
Do you need to learn about gas chromatography? This article takes you through the basic principles and instrumentation. With illustrations!
Read MoreHow FRET Works: A Guide to Visualizing Protein Interactions
Not sure what FRET is, or just need a refresher on how FRET works? Read our short guide to understand the usefulness of FRET for studying protein-protein interactions.
Read MoreA Step-by-step Guide to Designing Cas13 gRNAs
Designing Cas13 gRNAs is a bit different from the standard Cas9. Read this guide to learn how it differs, and get a step-by-step guide on designing the perfect Cas13 gRNAs.
Read MoreHow to Perform RNA Quality Control in the Lab: 3 Key Considerations
Discover what RNA quality control is, why it’s so important for your experiments, and how to undertake it with these 3 key considerations.
Read MoreHow Chromatography Works: Separation Science Explained with 5 Simple Examples
In biosciences, we purify our target molecules using chromatography. So, we need a thorough understanding of how chromatography works!
Read MoreMultiple Fragment Ligation: The Why and How
You may be familiar with standard single fragment ligations, but did you know you can ligate multiple fragments into your vector all at the same time! Discover how to perform multiple fragment ligation, including the different methods and troubleshooting tips for when things go wrong.
Read MoreMethods for Relative Quantification of qPCR Data: Yes, There is More Than One
Want to know the best way to perform relative quantification for your qPCR data? Read on.
Read MoreThe 4 Important Steps for Western Blot Quantification
Western blot quantification can show how protein expression changes between conditions and treatments. Discover the 4 critical steps you need for quantification.
Read MoreWhat is Touchdown PCR? A Primer and 5 Easy Tips For Success
Got non-specific PCR amplification? You need touchdown PCR! Discover what it is, how it works, and get 5 top tips for performing touchdown PCR.
Read More5 Ways to Destroy your Agarose Gel and How to Avoid Them!
Pouring and running an agarose gel should be a simple and routine procedure, but there are a surprising number of ways to destroy your agarose gel.
Read MoreCRISPR-Cas13: How to Revolutionize Your RNA Research
CRISPR isn’t just about DNA editing. Discover how you can use Cas13 proteins in your research to knock down, modify or track RNAs in mammalian cells.
Read MoreTop 5 Protein Quantification Assays
When working with proteins, accurate protein quantification is key for getting reliable data. Here are the top 5 quantification assays.
Read MoreThe qPCR Standard Curve: The Key to Good qPCR Data
Can’t be bothered doing a qPCR standard curve? Find out why it’s important to getting great results and how to do it.
Read MoreHow Using Oil Immersion Microscopy Can Increase Your Resolution
Oil immersion microscopy can improve your resolution in microscopy. This article will explain why this is the case and how you can use oil immersion microscopy in the lab!
Read MoreThe Basics of NMR Part 3: Multidimensional NMR
NMR is a powerful structural tool for studying proteins. Learn how to use multidimensional NMR to identify ligand binding sites and more!
Read MoreHow Confluent Are Your Cells? A Beginner’s Guide to Measuring Cell Culture Confluency
Cell confluency can affect cell behavior and growth. Find out what confluency is, how to measure it, and why it is a crucial consideration for your experiments.
Read MoreThe Basics of NMR Part 2: NMR and Protein Dimerization
NMR can generate tons of useful data for protein samples. Learn all about NMR and Protein Dimerization in this easy intro.
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