Techniques
Electron Microscopy Sample Preparation: 10 Pieces of Expert Advice
Do you prepare samples for electron microscopy and want to save time, money, effort, and frustration? This article provides hands-on advice to help you get the best possible data out of your EM experiments.
Read MoreOverhang PCR: Add Missing DNA Sequences Using Primers
Need to add extra nucleotides to your plasmid or other DNA sample? Here’s how to use overhang PCR to easily add extra bases using primers.
Read MoreA Better Alternative to Immortalized Cells: Combining CRISPR and iPSCs
Investigating human diseases and genetic variation is complex, but CRISPR-edited induced pluripotent stem cells present a promising alternative to immortalized cell lines. This article delves into genome editing principles and offers practical steps for optimizing research techniques, ensuring more accurate and ethical studies.
Read MoreHow to Preserve Microorganisms: Store Your Cells Better
We must preserve microorganisms for research continuity and to prevent frustrating delays. Here are several reliable ways to do it.
Read More4 Fixatives for Histology and Cytometry. Perfect Your Preservation
Learn about four fixatives for histology, which one you should pick, and how. Plus, get some top tips for perfect sample preservation.
Read More7 Top Tips to Make the Most of Your Flow Cytometry Training
So you’ve got your flow cytometry training booked and are one step closer to that precious data. Read our 7 top tips on how to get the most from your flow cytometry training.
Read MoreConfocal Laser Scanning Microscopy Explained In 3 Easy Steps
Learn how confocal laser scanning microscopy works, its applications, and why it’s great for samples that are too thin to section.
Read More8 Cell Lysis Methods to Break Cell Walls
We must lyse cells to extract our samples, but which cell lysis method is best for your cells? Read this article to learn about eight excellent methods.
Read MoreA Beginner’s Guide to Protein Structure Prediction
Dive into the world of protein structure science, where AI is revolutionizing the field by offering accurate predictions and free, open-access tools. In this beginner’s guide, we’ll explore these groundbreaking tools, discuss their strengths and weaknesses, and examine the implications for traditional experimental methods like NMR and X-ray crystallography.
Read MoreAn Easy and Reliable Nuclear Extraction Protocol
Here’s your reliable and adaptable nuclear extraction protocol. It contains buffer recipes, explanations of what the reagents do, and top tips to boost yields.
Read MoreGenetic Variants Explained
Genetic variants are critical to fields like evolution, diagnostics, and medicine—but they’re complex. This article breaks them down.
Read MoreMaxam–Gilbert Sequencing: What It Is and 3 Modern Applications
Maxam–Gilbert Sequencing. Slow and obsolete or niche but powerful? Discover how it works and learn about three modern applications.
Read MoreMaximizing Protein Extraction from Yeast Cells: A Comparison of 4 Methods
Confused about yeast protein extraction methods? Let this article guide you through the process and help you select the most appropriate method based on your research goals and available resources.
Read MoreWhat Reagents Can You Use Past Their Chemical Expiry Date?
Learn about what reagents are usable past their chemical expiry date, how can you check if they are still okay, and which ones you should throw out.
Read MoreA Beginner’s Guide to How Blunt-End Cloning Works
Struggling with your cloning? It could be time to try blunt-end cloning. This easy introduction explains what it is, when to use it, and how.
Read MoreHow to Totally Nail Your First in situ Hybridization
Having problems with your in situ hybridization? We’ve got 7 simple tips to help you get outstanding results.
Read More5 Controls for Immunofluorescence: A Beginner’s Guide
Achieving publication-quality immunofluorescence images is tricky. Learn what controls for immunofluorescence you can use to get them!
Read MoreWhat is Water of Crystallization? Everything You Need to Know
Not sure what water of crystallization is? This article explains everything you need to know so that you’re equipped with the knowledge to decide if it matters to your experiments.
Read MoreThe 2 Types of Digital Images: Tools to Prepare Stunning Images for Publication
Digital images are essential to communicate your data. Get the information and tools you need to take your scientific illustration to the next level!
Read MoreWhat Is Protein Crystallography? Structure Solution Explained
What is protein crystallography? Whether you are about to enter the world of protein crystallography or work with a protein crystallographer on your grant, this article will be useful.
Read MoreProtein Colocalization: 2 Essential Methods to Prove Protein Overlap
You know the drill. To prove your theory, you must show the colocalization of X and Y in a cell. Here are 2 ways to reveal protein colocalization.
Read MoreWhat Ponceau S Staining Can Reveal About Your Western Blot
Discover how Ponceau S works, how to perform staining, and get a detailed guide to interpreting what the staining means when troubleshooting your failed blots.
Read MoreFluorescence Microscopy: From Principles to Applications
Whether you want to get started with fluorescence microscopy or already use it, this guide will ensure you know the basics and get the best out of your fluorescence microscopy.
Read MoreHow to Build Your Own PCR Machine
You can build your own PCR machine using tools and equipment you can get easily and without breaking the bank. Well, what are you waiting for?
Read MoreHow To Make ECL Reagent: 4 Ingredients, Better Blots
ECL is an expensive reagent. Why not learn how to make ECL yourself? This cheap and simple option will give you better blots more often!
Read MoreHow To Passage Cells in Culture
Learn the basics of how to passage cells in culture with this easy-to-follow guide. While the specifics change per cell line, the basics remain.
Read More12 Top Tips for Working in a Biosafety Cabinet
Biosafety cabinets keep your work safe from contamination, and you safe from your work. So here are 12 tips for Working in a biosafety cabinet!
Read MoreNail Your Unmixing for Full Spectrum Flow Cytometry: 7 Top Tips
Spectral unmixing in flow cytometry is the key to great data from your full spectrum flow cytometry. Get this wrong, and you risk unreliable results. Read our top 7 tips from a flow cytometry core facilities expert to nail your unmixing.
Read MoreWhat’s in Your Cell Culture Medium?
Do you know what each ingredient in cell culture media does? Read on to take a look at the common ingredients and break down their roles.
Read More3 Hot Tips to Optimize Your Western Blot Transfers
Are you struggling with ugly and unreadable western blots? Here are 3 ways to optimize your western blot transfer and get blots to be proud of.
Read MoreUnderstanding Cell Passage Number and How to Calculate it for Cell Cultures
Not sure what we mean by cell passage number? Confused about how to calculate it? Wondering if there is a maximum number? We explain it all.
Read MoreHow to Measure Molecular Weight Using Size-Exclusion Chromatography (SEC)
We often use size-exclusion chromatography to purify samples. Read this article to learn how to use it to measure sample molecular weight as well!
Read MoreHPLC and GC: 6 Fundamental Differences Made Simple to Enhance Your Research
Need to get a handle on the fundamental differences between HPLC and GC to pick one for your studies? Here’s a quick explainer!
Read MoreTop 5 of the Most Commonly Used Cell Lines
Want to use a cell line but not sure where to start? Or perhaps you’re just curious about the most commonly used cell lines. Our top 5 most commonly used cell lines will help you get a feel for the cells that many researchers turn to.
Read MoreGoing Serum-Free: The How and Why of Removing Serum From Your Media
While using serum in cell culture can keep your cells healthy and happy, there are some notable downsides. We discuss the pros and cons of using serum in cell culture media and how to remove it if you want to eliminate it from your workflow.
Read MoreGas Chromatography: What It Is, How It Works, and 5 Critical Components
Do you need to learn about gas chromatography? This article takes you through the basic principles and instrumentation. With illustrations!
Read MoreHow FRET Works: A Guide to Visualizing Protein Interactions
Not sure what FRET is, or just need a refresher on how FRET works? Read our short guide to understand the usefulness of FRET for studying protein-protein interactions.
Read MoreA Step-by-step Guide to Designing Cas13 gRNAs
Designing Cas13 gRNAs is a bit different from the standard Cas9. Read this guide to learn how it differs, and get a step-by-step guide on designing the perfect Cas13 gRNAs.
Read MoreHow to Perform RNA Quality Control in the Lab: 3 Key Considerations
Discover what RNA quality control is, why it’s so important for your experiments, and how to undertake it with these 3 key considerations.
Read MoreHow Chromatography Works: Separation Science Explained with 5 Simple Examples
In biosciences, we purify our target molecules using chromatography. So, we need a thorough understanding of how chromatography works!
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