Techniques
Xeno Nucleic Acids: Essential Tools in Synthetic Biology and Your Research
We’re all familiar with DNA and RNA. But have you heard of xeno nucleic acids (XNAs)? Read on to find out how they can be applied in biological research, and how you can start using them in your experiments.
Read MoreHow DNA Extraction Kits Work in the Lab
Understanding how DNA extraction kits work is the key to troubleshooting your extraction issues.
Read MoreLight-up RNA Aptamers: Illuminating the World of RNA
Discover how you can visualize that notoriously difficult molecule, RNA using light-up RNA aptamers (LURAs).
Read MoreIntroduction to DNA Microarrays
This is the first installment in the DNA microarray series where I will introduce the technology and explain the basics.
Read MoreChoosing the Right Bacterial Strains for Your Lab
Whether you’re simply seeking competent E. coli for a transformation experiment or building a panel intended for a whole project, it’s vital that you pick the right strain for your experiment. Find out more in our complete guide to choosing bacterial strains.
Read MorePractical Application of Phenol-Chloroform Extraction
Are you struggling with your phenol-chloroform extraction or just looking to maximize the nucleic acid you retrieve? Then read our expert advice to improve your technique.
Read MoreThe Basics: How Phenol Extraction of DNA Works
Phenol extraction is a common method for removing proteins from nucleic acids. Discover how phenol extraction of DNA works.
Read MoreTranslational Cancer Research
Translational Cancer ResearchSee the Hidden – LIVE Online Workshop Date: Thursday 28th October 2021Time: LONDON 10:00 – 15:00 | BERLIN 11:00 – 16:00 | DUBAI 13:00 – 18:00 Understanding cancer biology has become increasingly dependent on imaging. High-resolution imaging is vital for studying genetic and cell signaling changes that underlie cancer, whereas live-cell imaging is…
Read MoreAll Charged Up: The Basics of Ion-Exchange Chromatography
Ion-exchange chromatography is used to separate and purify proteins based on net charge at a particular pH. Here are the basics about this technique.
Read MoreMeasuring Beyond the Limitations of ELISAs
Measuring Beyond the Limitations of ELISAs Date: Tuesday November 16th 2021Time: 8am Los Angeles, 11am New York, 4pm London, 5pm Berlin Speaker Craig Lanio Field Application Scientist, MilliporeSigma Craig is a Field Application Scientist with MilliporeSigma and has been part of the Immunoassay Platform Solutions team for almost 15 years. Prior to coming to MilliporeSigma, he…
Read MoreTissue Processing For Histology: What Exactly Happens?
Tissue processing for histology is a key step between fixation and embedding. We take you through the steps of tissue processing in this simple guide.
Read MoreHyperplex cancer tissue analysis at single cell level with Cell DIVE™
Hyperplex cancer tissue analysis at single cell level with Cell DIVE™ Available On Demand The H2P2 histopathology platform team have been mastering immunohistochemical staining and multiplexing for more than 15 years. With Cell DIVE technology, they offer the scientific community the opportunity to perform hyperplex labeling with up to 50 labels on a single tissue…
Read MoreUsing a Gel Filtration Chromatogram to Estimate Molecular Weight
Do you need a way to estimate the molecular weight of a sample or protein? We walk you through one simple solution using a gel filtration chromatogram.
Read MoreThe Basics of Running a Chromatography Column
Are you preparing to run your first column chromatography experiment? Here are some basics that you should know before you use that precious sample.
Read MoreDIY Electrocompetent E. coli
Making good quality electrocompetent E. coli is very easy. One morning’s work (with a bit of work ahead of time) is all it takes.
Read MoreWhat the HEK? A Beginner’s Guide to HEK293 Cells
If you’re at all familiar with cell culture, you’ve probably heard of HEK293 cells. HEK293 cells are one of the most commonly used cell lines in laboratories across the globe. But what are they? Why should you use them? And what does the ‘293’ mean? Are HEK293T cells the same thing? Read on for answers…
Read MoreFunctional Genomics Screening: From Knockout and Modulation to Single Cell Analysis
Functional Genomics Screening: From Knockout and Modulation to Single Cell Analysis Date: Wednesday November 3rd, 2021Broadcast 1: 9am Moscow, 10am Dubai, 11:30am Delhi, 2pm Singapore, 5pm SydneyBroadcast 2: 9am Los Angeles, 11am Chicago, 12pm New York, 4pm London, 5pm Berlin Speaker Gurpreet Balrey, PhD Head of Global Commercial Enablement and EMEA Business HeadMerck KGaA, Darmstadt,…
Read MoreThe Luciferase Reporter Assay: How it Works
Discover how the luciferase reporter assay works and how to get starting using it in your research.
Read MoreWhat is Alternative Splicing, and Why Is It Important?
Need to brush up on your alternative splicing knowledge? We’re here to help with our guide to this splicing mechanism.
Read MoreEssential Factors for Successful Gateway Cloning
Want to use Gateway cloning or having trouble using this technology? Find out how it works and get helpful tips to increase your success.
Read MoreThe Ultimate Guide to Choosing a Fluorescent Protein
Discover the critical considerations when choosing a fluorescent protein, the key features of those most commonly used, and why newer might be better.
Read MoreHigh-resolution 3D imaging uncovers vascular control of tissue ageing
High-resolution 3D imaging uncovers vascular control of tissue ageing Wednesday 15th September 2021LONDON 16:00 BST | BERLIN 17:00 CET | DUBAI 19:00 GST Ageing is associated with alterations in tissue homeostasis and a decline in tissue functions. Blood vessels provide supportive microenvironments for various cell types to maintain tissue homeostasis and organ function. However, ageing…
Read MoreA Step-by-Step Guide to the Quantitative Beta-galactosidase Assay for Y2H Screening
Want to know not only if your proteins bind but quantify the interaction? We walk you through how to perform a quantitative yeast two-hybrid assay.
Read MoreThe Three Ts of Introducing Foreign DNA: Transfection, Transduction, and Transformation
Do you know your transfections from your transductions? We explain the differences between three of the most commonly used ways of introducing foreign DNA.
Read MoreBeat Back the Bubbles: 6 Ways to Remove Excess Detergent from Your Membrane Protein Sample
Excess detergent in your membrane proteins samples can cause you serious headaches. We’ve got you covered with 6 ways you can remove excess detergent from membrane proteins.
Read MoreDNA Precipitation: Ethanol vs. Isopropanol
Discover what you need for successful DNA precipitation and how to choose between ethanol and isopropanol solvents.
Read MoreIn the right place at the right time: How to successfully implement the Leica Nano workflow
In the right place at the right time:How to successfully implement the Leica Nano workflow Tuesday September 7th 2021LONDON 15:00 BST | BERLIN 16:00 CET | DUBAI 18:00 GST Many electron microscope (EM) workflows start with sample fixation, followed by sample preparation and EM imaging. However, samples displaying interesting behavior tend to be rare, and…
Read MoreHow SDS-PAGE Works
Knowing how SDS-PAGE works means that you can troubleshoot any issues in your experiment and tweak the setup to get publication-worthy figures. Find out how it works here.
Read MoreAn Introduction To Fixation For Histology: Think Before You Fix!
How you fix your tissue or cells can affect your results, for better or for worse. Discover the key points to think about before undertaking your histology fixation.
Read MoreA (very) Short History of Histology
Discover the history of histology, from the first mention of a cell in 1665 to the identification and development of various stains.
Read MoreAvoid These Pitfalls: Seven (Not So Deadly) Histology Sins
Discover seven common histology mistakes and how you can avoid making them when performing your experiments.
Read MoreIs Your Bacterial Culture Still Growing? A Primer on OD600 Measurements
How do you know when your bacterial culture is “done” growing? Read on to learn how to monitor bacterial growth using OD600 measurements, and how to figure out when you should harvest your culture.
Read MoreCrystallize Your Protein–DNA Complex by Optimizing These Key Parameters
Looking to engineer a crystal? Here are some key parameters to optimize to give you the maximum chance of success at crystallizing protein DNA–complexes.
Read MoreGetting Started with Immunohistochemistry
Immunohistochemistry isn’t just a useful clinical tool, it also has great applications as a basic research tool. We’ll walk you through the immunohistochemistry basics to get you off to a flying start.
Read MoreAutomating IHC and RNAscope® ISH for academic research: Insights from equine arteritis virus and COVID-19
Automating IHC and RNAscope® ISH for academic research: Insights from equine arteritis virus and COVID-19 Available On Demand Speaker Dr. Mariano Carossino DVM, PhD, Dipl. ACVM Assistant Professor (appointed) Department of Pathobiological Sciences & Louisiana Animal Disease Diagnostic LaboratoryLouisiana State University, School of Veterinary Medicine Dr. Mariano Carossino is an Assistant Professor at the Louisiana…
Read MoreThat Other Number – The Meaning Of Numerical Aperture In Microscopy
Do you know what that NA number is on your objective? We walk you through what the numerical aperture is and why it’s important.
Read MoreAn Objective View: What do These Abbreviations Mean on Microscope Objectives?
There are a large number of microscope objective abbreviations relating to optical aberrations; here we’ll shed some light on some of the most common ones to get you up to speed in no time!
Read MoreImproving the Expression of Insoluble and Inactive Proteins Using Osmotic Shock
Discover a better way to improve the expression of your insoluble and inactive proteins using osmotic shock.
Read MoreIntroduction to Yeast Media: How to Feed Your Fungus
Are you finding the variety of available yeast media overwhelming? Read our guide to understand what’s in each and which is right for your fungus.
Read MoreCell Counting with a Hemocytometer: As easy as 1, 2, 3…
Do hemocytometers look scary and complicated with their multiple tiny squares, boxes, and grids? Take a look at our article and see how easy it actually is to use a hemocytometer.
Read More