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Techniques

What Is a Cq (Ct) Value?

Don’t be confused by Ct values! We’ll guide you through what they are, how to calculate them, and troubleshooting issues.

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The Essential Guide to In-Cell Westerns

In-cell Westerns are a powerful technique that has enhanced how researchers analyze protein expression levels and signaling pathways within fixed cells. Learn about their primary advantages, applications, and some of the best tech and products to perform them.

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The Beginner’s Guide to Reading Plasmid Maps

Plasmid maps are a cornerstone of biology, but they are confusing to read for beginners. Our easy guide tells you how to read them, where to look for essential information, and how to avoid common mistakes.

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Plasmid vs. Genomic DNA Extraction: The Difference

Most experiments start with a piece of DNA—either plasmid DNA or genomic DNA. And your downstream uses for it dictate how much you need, what contaminants you can tolerate, and your extraction and purification methods. In this article, we explain the key differences between plasmid and genomic DNA extraction methods.

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A Better Alternative to Immortalized Cells: Combining CRISPR and iPSCs

Investigating human diseases and genetic variation is complex, but CRISPR-edited induced pluripotent stem cells present a promising alternative to immortalized cell lines. This article delves into genome editing principles and offers practical steps for optimizing research techniques, ensuring more accurate and ethical studies.

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A Beginner’s Guide to Protein Structure Prediction

Dive into the world of protein structure science, where AI is revolutionizing the field by offering accurate predictions and free, open-access tools. In this beginner’s guide, we’ll explore these groundbreaking tools, discuss their strengths and weaknesses, and examine the implications for traditional experimental methods like NMR and X-ray crystallography.

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Genetic Variants Explained

Genetic variants are critical to fields like evolution, diagnostics, and medicine—but they’re complex. This article breaks them down.

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How to Build Your Own PCR Machine

You can build your own PCR machine using tools and equipment you can get easily and without breaking the bank. Well, what are you waiting for?

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How To Passage Cells in Culture

Learn the basics of how to passage cells in culture with this easy-to-follow guide. While the specifics change per cell line, the basics remain.

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