Genomics and Epigenetics
Quick and Easy Sequencing of SARS-CoV-2 to Identify Variants of Concern
Quick and Easy Sequencing of SARS-CoV-2 to Identify Variants of Concern Date: Tuesday June 22nd 2021Time: 8am Los Angeles, 11am New York, 4pm London, 5pm Berlin Speaker Jordan RoseFigura, PhD Director of Material SciencesSwift Biosciences Dr. Jordan RoseFigura completed her Ph.D. in Chemistry at UC Berkeley where she studied the mechanism of PQQ formation. She…
Read More Multiplex CRISPR Gene Editing: What You Need to Know
If you need a multi-gene knockout or large-scale genomic modification, or want reduced off-target effects, then multiplex CRISPR is for you!
Read More Controlling Gene Expression with CRISPR Interference (CRISPRi)
CRISPR interference allows for the regulation of gene expression in vivo. Here’s a short guide to how it works.
Read More CRISPR-Cas9 Genome Editing: Weighing the Pros and Cons
Screening in the Modern CRISPR Era
Screening in the Modern CRISPR Era Available On Demand Speaker Andrew Ravanelli Principal Scientist, Genome and Epigenome EditingMerck KGaA, Darmstadt, Germany Andrew obtained his Ph.D. in Cell Biology from Duke University, where he used mouse and zebrafish models to dissect cellular mechanisms of early tissue formation. He completed a postdoctoral fellowship in Pediatrics at the…
Read More CRISPR Screening: from design and discovery to validation
CRISPR Screening: from design and discovery to validation On Demand Speaker Gillian Browne, PhD Global Head of Commercial Enablement, Genome Engineering & ModulationMilliporeSigma Gillian received a BSc in Biomedical Sciences, followed by a PhD in Cellular and Molecular Cancer Biology in the UK. She pursued five years of postdoctoral research in the USA and during…
Read More Optimizing CRISPR mouse model pipelines – modified synthetic sgRNAs
Optimizing CRISPR mouse model pipelines – modified synthetic sgRNAs On Demand Speaker Matthew Mackenzie Resource Support ManagerMRC Harwell Matthew Mackenzie earned a BSc in Genetics at the University of Liverpool, where he spent a year in industry with LGC Forensics (now Eurofins) gaining insights into high-throughput sample analysis and automation. After graduating in 2015 he…
Read More A guide to leveraging single cell CRISPR screens for deeper biological insights
A guide to leveraging single cell CRISPR screens for deeper biological insights On Demand Speakers Andrew Ravanelli, Ph.D. Senior R&D Scientist, Genome and Epigenome Editing, Merck KGaA, Darmstadt, Germany Andrew obtained his Ph.D. in Cell Biology from Duke University, where he used mouse and zebrafish models to dissect cellular mechanisms of early tissue formation. He…
Read More Size Analysis of High-Molecular-Weight DNA for Long-Read Sequencing
Discover how to check DNA quality for long-read sequencing using electrophoresis and why pipetting carefully is so important.
Read More Markerless and Scarless Microbial Genome Engineering
Markerless and Scarless Microbial Genome Engineering On Demand Webinar Speaker Erik Eastlund Principal Scientist, Gene Editing and Novel Modalities, Merck KGaA, Darmstadt, Germany Erik is a principal scientist for Gene Editing and Novel Modalities at Merck KGaA, Darmstadt, Germany. His group is focused on developing microbial genome engineering tools while also engaging in a microbiome…
Read More A Brief History of CRISPR-Cas9 Genome-Editing Tools
Learn how the CRISPR prokaryote immune response systems were first discovered and the development of the CRISPR-Cas9 gene-editing tool.
Read More Level Up Your Drug Screening With CRISPR
Efficient Generation of Gene-edited Mouse Models and Cell Lines Using Synthetic sgRNA
Efficient Generation of Gene-edited Mouse Models and Cell Lines Using Synthetic sgRNA On Demand Webinar Dr. Peter Romanienko Managing Director, Genome Editing Shared Resource, Rutgers-Cancer Institute of New Jersey Dr. Peter Romanienko obtained his PhD training from Cornell Graduate School of Medical Sciences/Sloan Kettering Institute working on DNA repair in Dr. Maria Jasin’s laboratory and…
Read More Why You Should Consider Adding CRISPRa and CRISPRi to Your Toolbox
Find out how CRISPR-mediated gene activation (CRISPRa) and repression (CRISPRi) works and why you should consider using them in addition to your CRISPR knockouts.
Read More How CRISPR Can Be Used to Detect Emerging Viral Pathogens
A CRISPR Approach to Immuno-Oncology
T cells can be tricky to engineer with CRISPR. Find out the key considerations when editing these cells and how you can overcome any associated challenges.
Read More Plant vs. Virus: Comparative transcriptomic analysis of single TuMV-infected micro-dissected plant cells
Plant vs. Virus: Comparative transcriptomic analysis of single TuMV-infected micro-dissected plant cells In this tutorial, you will find: How studying the early events of virus infection in plants avoids the dilution effect and allows identification of the key molecular players involved in initial plant-virus interactions. How the MMI CellCut microdissection system allows dissection of the…
Read More How to Understand CRISPR Formats and Their Applications
Find out how modified variants of CRISPR nucleases provide gene editing with reduced off-target effects and can even control gene expression without altering the DNA sequence.
Read More How to Validate a CRISPR Experiment
Discover how to validate your CRISPR gene editing, from the successful delivery of CRISPR reagents to the confirmation of desired genetic and phenotype changes.
Read More CRISPR Gene Editing: Considerations and Getting Started
Discover how to get started with CRISPR gene editing in your experiments with our key considerations.
Read More Engineering Vero Cell Lines Using CRISPR to Increase Production of Viral Vaccines
Engineering Vero Cell Lines Using CRISPR to Increase Production of Viral Vaccines On Demand Webinar Dr. Benjamin Borgo Dr. Benjamin Borgo currently leads a team of forward-thinking product and service managers within Merck KGaA’s Genome Engineering and Modulation franchise. His first exposure to CRISPR-based genome editing was as a graduate student where he attempted to…
Read More 3′ mRNA-Seq is Transforming Gene Expression Analysis
3′ mRNA-seq is a NGS method to profile gene expression that is sensitive and straightforward but won’t blow your budget.
Read More Bioinformatics: It’s Not All About Genomics
Getting Started with NGS Bioinformatics
Bioinformatics and NGS go together like peanut butter and jelly. But if you’re just starting out with these techniques it can be daunting.
Read More WGS Workflow: From Sample Collection to Data Interpretation
The efficiency of whole genome sequencing (WGS) workflows has skyrocketed since its inception. Major leaps and minor tweaks in the WGS workflow have compounded over time resulting in radical reductions in processing time and the cost of sequencing whole genomes over the past decades. The complete sequencing of the first human genome, named the Human…
Read More The Recipe for Successful Whole Genome Sequencing
The success of whole genome sequencing (WGS) is shown in the quick and efficient scientific response to the 2011 outbreak of E. coli in Germany and France.1 German and French strains of E. coli were indistinguishable using standard tests. However, WGS analysis showed 2 single nucleotide polymorphisms (SNPs) in the German strains and 9 SNPs…
Read More CRISPR Nucleases: The Ultimate Guide To Selection
Confused about CRISPR nucleases? Read this guide to discover the various CRISPR nucleases available and what they are best suited for.
Read More A Colorful Route to Sequencing Success
An oft-repeated maxim in biological bench science is that any experiment is only as good as its control. A control is an unchanging standard of comparison in an experiment, and an internal control is typically a standard reaction run together with the test reaction in the same reaction mixture. The purpose of an internal control…
Read More CRISPR Multiplexing Strategies: What’s The Choice?
Although multiplex CRISPR gene editing can be accomplished by simply introducing more than one gRNA to your target cells, there are many alternative — and more efficient — ways of achieving this goal. This article discusses these alternative CRISPR multiplexing strategies and highlights their potential caveats. Not sure whether multiplex CRISPR gene editing is right…
Read More Dead Useful: CRISPR-Cas9 Epigenome Editing
Want to do some epigenome editing? Discover the usefulness of catalytically inactive (dead) Cas9.
Read More DNA Extraction for Next Gen Sequencing
The advent of Next Gen Sequencing (NGS) has been truly amazing. One of the marvels that is often overlooked is how advances in DNA extraction technology have helped streamline NGS workflows. The original standard – phenol/chloroform extraction – is not well suited to the automated nature of today’s sequencing workflows (though with the emergence of…
Read More Get Prepped: Nanopore Library Preparation Optimization
Nanopore is a relatively new sequencing platform and researchers are still trying to optimize the protocol for their own specific applications. In our lab, we work primarily with metagenomic samples and use the 1D sequencing kits. Over the past year, we have optimized this technique. To check the quality of the Nanopore library preparation we…
Read More CRISPR-Based Activation (CRISPRa): A How-To Guide
CRISPRa allows you to activate or overexpress genes in a more endogenous manner. Find out the steps to getting started.
Read More What’s that Organism? Using DNA Barcoding for Species Identification
In both the lab and field, it is important to know what species we are working with. While morphological data has always been a tried and true method of identifying species, DNA barcoding allows us to identify species when we don’t have that option (e.g. if we don’t have enough of a specimen to identify…
Read More How to Confirm Your CRISPR-cas9 Genome Editing Was Successful
Level-up your troubleshooting ability by determining the success of failure of each stage of your CRISPR experiment.
Read More Use ddRAD-seq to Study Non-Model Organisms
Reduced-representation genome sequencing has been one of the most important advances in the last several years for enabling massively parallel genotyping of organisms for which there is no reference-grade genome assembly. An implementation of the approach known as ddRAD-seq, first conceived in the Hoekstra lab at Harvard, has been widely adopted by the plant and…
Read More Reducing GC Bias in WGS: Moving Beyond PCR
WGS technologies have seen significant progress since the completion of the Human Genome Project in 2003. First-generation Sanger Sequencers were limited by lengthy run times, high expenses, and throughputs that read only tens of kilobases per run. The arrival of second-generation sequencers in the mid-2000s brought about the plummeting of sequencing costs and run times,…
Read MoreHow to Improve Your WGS DNA Library
In whole genome sequencing (WGS) initiatives it is not enough to simply sequence the whole length of the genomic DNA sample just once. This is because genomes are usually very large. The human genome, for example, contains approximately 3 billion base pairs. Although sequencing accuracy for individual bases is very high, when you consider large…
Read More Generating RNA-seq Libraries from RNA
One of the most powerful methods of modern cellular biology is creating and analyzing RNA libraries via RNA-sequencing (RNA-seq). This technique, also called whole transcriptome shotgun sequencing, gives you a snapshot of the transcriptome in question, and can be used to examine alternatively spliced transcripts, post-transcriptional modifications, and changes in gene expression, amongst other applications.…
Read More Battle of the Methods: Whole Transcriptome Versus mRNA-seq
Maybe you want to examine the entire transcriptome or maybe you want to investigate changes in expression from your favorite gene. You could do whole transcriptome sequencing or mRNA-seq. But which one is right for your project? From budget considerations to sample collection, let’s briefly look at both to see which might be best for your…
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