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Genomics and Epigenetics

Quick and Easy Sequencing of SARS-CoV-2 to Identify Variants of Concern

Quick and Easy Sequencing of SARS-CoV-2 to Identify Variants of Concern Date: Tuesday June 22nd 2021Time: 8am Los Angeles, 11am New York, 4pm London, 5pm Berlin Speaker Jordan RoseFigura, PhD Director of Material SciencesSwift Biosciences Dr. Jordan RoseFigura completed her Ph.D. in Chemistry at UC Berkeley where she studied the mechanism of PQQ formation. She…

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Screening in the Modern CRISPR Era

Screening in the Modern CRISPR Era Available On Demand Speaker Andrew Ravanelli Principal Scientist, Genome and Epigenome EditingMerck KGaA, Darmstadt, Germany Andrew obtained his Ph.D. in Cell Biology from Duke University, where he used mouse and zebrafish models to dissect cellular mechanisms of early tissue formation. He completed a postdoctoral fellowship in Pediatrics at the…

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CRISPR Screening: from design and discovery to validation

CRISPR Screening: from design and discovery to validation On Demand Speaker Gillian Browne, PhD Global Head of Commercial Enablement, Genome Engineering & ModulationMilliporeSigma Gillian received a BSc in Biomedical Sciences, followed by a PhD in Cellular and Molecular Cancer Biology in the UK. She pursued five years of postdoctoral research in the USA and during…

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Optimizing CRISPR mouse model pipelines – modified synthetic sgRNAs

Optimizing CRISPR mouse model pipelines – modified synthetic sgRNAs On Demand Speaker Matthew Mackenzie Resource Support ManagerMRC Harwell Matthew Mackenzie earned a BSc in Genetics at the University of Liverpool, where he spent a year in industry with LGC Forensics (now Eurofins) gaining insights into high-throughput sample analysis and automation. After graduating in 2015 he…

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A guide to leveraging single cell CRISPR screens for deeper biological insights

A guide to leveraging single cell CRISPR screens for deeper biological insights On Demand Speakers Andrew Ravanelli, Ph.D. Senior R&D Scientist, Genome and Epigenome Editing, Merck KGaA, Darmstadt, Germany Andrew obtained his Ph.D. in Cell Biology from Duke University, where he used mouse and zebrafish models to dissect cellular mechanisms of early tissue formation. He…

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Markerless and Scarless Microbial Genome Engineering

Markerless and Scarless Microbial Genome Engineering On Demand Webinar Speaker Erik Eastlund Principal Scientist, Gene Editing and Novel Modalities, Merck KGaA, Darmstadt, Germany Erik is a principal scientist for Gene Editing and Novel Modalities at Merck KGaA, Darmstadt, Germany. His group is focused on developing microbial genome engineering tools while also engaging in a microbiome…

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Efficient Generation of Gene-edited Mouse Models and Cell Lines Using Synthetic sgRNA

Efficient Generation of Gene-edited Mouse Models and Cell Lines Using Synthetic sgRNA On Demand Webinar Dr. Peter Romanienko Managing Director, Genome Editing Shared Resource, Rutgers-Cancer Institute of New Jersey Dr. Peter Romanienko obtained his PhD training from Cornell Graduate School of Medical Sciences/Sloan Kettering Institute working on DNA repair in Dr. Maria Jasin’s laboratory and…

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Plant vs. Virus: Comparative transcriptomic analysis of single TuMV-infected micro-dissected plant cells

Plant vs. Virus: Comparative transcriptomic analysis of single TuMV-infected micro-dissected plant cells In this tutorial, you will find: How studying the early events of virus infection in plants avoids the dilution effect and allows identification of the key molecular players involved in initial plant-virus interactions.  How the MMI CellCut microdissection system allows dissection of the…

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Engineering Vero Cell Lines Using CRISPR to Increase Production of Viral Vaccines

Engineering Vero Cell Lines Using CRISPR to Increase Production of Viral Vaccines On Demand Webinar Dr. Benjamin Borgo Dr. Benjamin Borgo currently leads a team of forward-thinking product and service managers within Merck KGaA’s Genome Engineering and Modulation franchise. His first exposure to CRISPR-based genome editing was as a graduate student where he attempted to…

Production of Viral Vaccine can be enhanced using CRISPR genome-editingRead More

WGS Workflow: From Sample Collection to Data Interpretation

The efficiency of whole genome sequencing (WGS) workflows has skyrocketed since its inception. Major leaps and minor tweaks in the WGS workflow have compounded over time resulting in radical reductions in processing time and the cost of sequencing whole genomes over the past decades. The complete sequencing of the first human genome, named the Human…

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The Recipe for Successful Whole Genome Sequencing

The success of whole genome sequencing (WGS) is shown in the quick and efficient scientific response to the 2011 outbreak of E. coli in Germany and France.1 German and French strains of E. coli were indistinguishable using standard tests.  However, WGS analysis showed 2 single nucleotide polymorphisms (SNPs) in the German strains and 9 SNPs…

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A Colorful Route to Sequencing Success

An oft-repeated maxim in biological bench science is that any experiment is only as good as its control. A control is an unchanging standard of comparison in an experiment, and an internal control is typically a standard reaction run together with the test reaction in the same reaction mixture. The purpose of an internal control…

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CRISPR Multiplexing Strategies: What’s The Choice?

Although multiplex CRISPR gene editing can be accomplished by simply introducing more than one gRNA to your target cells, there are many alternative — and more efficient — ways of achieving this goal. This article discusses these alternative CRISPR multiplexing strategies and highlights their potential caveats. Not sure whether multiplex CRISPR gene editing is right…

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DNA Extraction for Next Gen Sequencing

The advent of Next Gen Sequencing (NGS) has been truly amazing. One of the marvels that is often overlooked is how advances in DNA extraction technology have helped streamline NGS workflows. The original standard – phenol/chloroform extraction – is not well suited to the automated nature of today’s sequencing workflows (though with the emergence of…

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Get Prepped: Nanopore Library Preparation Optimization

Nanopore is a relatively new sequencing platform and researchers are still trying to optimize the protocol for their own specific applications. In our lab, we work primarily with metagenomic samples and use the 1D sequencing kits. Over the past year, we have optimized this technique. To check the quality of the Nanopore library preparation we…

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What’s that Organism? Using DNA Barcoding for Species Identification

In both the lab and field, it is important to know what species we are working with. While morphological data has always been a tried and true method of identifying species, DNA barcoding allows us to identify species when we don’t have that option (e.g. if we don’t have enough of a specimen to identify…

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Use ddRAD-seq to Study Non-Model Organisms

Reduced-representation genome sequencing has been one of the most important advances in the last several years for enabling massively parallel genotyping of organisms for which there is no reference-grade genome assembly. An implementation of the approach known as ddRAD-seq, first conceived in the Hoekstra lab at Harvard, has been widely adopted by the plant and…

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Reducing GC Bias in WGS: Moving Beyond PCR

WGS technologies have seen significant progress since the completion of the Human Genome Project in 2003. First-generation Sanger Sequencers were limited by lengthy run times, high expenses, and throughputs that read only tens of kilobases per run. The arrival of second-generation sequencers in the mid-2000s brought about the plummeting of sequencing costs and run times,…

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How to Improve Your WGS DNA Library

In whole genome sequencing (WGS) initiatives it is not enough to simply sequence the whole length of the genomic DNA sample just once. This is because genomes are usually very large. The human genome, for example, contains approximately 3 billion base pairs. Although sequencing accuracy for individual bases is very high, when you consider large…

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Generating RNA-seq Libraries from RNA

One of the most powerful methods of modern cellular biology is creating and analyzing RNA libraries via RNA-sequencing (RNA-seq). This technique, also called whole transcriptome shotgun sequencing, gives you a snapshot of the transcriptome in question, and can be used to examine alternatively spliced transcripts, post-transcriptional modifications, and changes in gene expression, amongst other applications.…

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Battle of the Methods: Whole Transcriptome Versus mRNA-seq

Maybe you want to examine the entire transcriptome or maybe you want to investigate changes in expression from your favorite gene. You could do whole transcriptome sequencing or mRNA-seq. But which one is right for your project? From budget considerations to sample collection, let’s briefly look at both to see which might be best for your…

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