Genomics and Epigenetics
A guide to leveraging single cell CRISPR screens for deeper biological insights
A guide to leveraging single cell CRISPR screens for deeper biological insights Date: Tuesday, October 6th 2020Time: 4 pm (London) 11 am (New York) 8 am (San Diego) Speakers Andrew Ravanelli, Ph.D. Senior R&D Scientist, Genome and Epigenome Editing, Merck KGaA, Darmstadt, Germany Andrew obtained his Ph.D. in Cell Biology from Duke University, where he used…
Read More Size Analysis of High-Molecular-Weight DNA for Long-Read Sequencing
Discover how to check DNA quality for long-read sequencing using electrophoresis and why pipetting carefully is so important.
Read More Markerless and Scarless Microbial Genome Engineering
Markerless and Scarless Microbial Genome Engineering On Demand Webinar Speaker Erik Eastlund Principal Scientist, Gene Editing and Novel Modalities, Merck KGaA, Darmstadt, Germany Erik is a principal scientist for Gene Editing and Novel Modalities at Merck KGaA, Darmstadt, Germany. His group is focused on developing microbial genome engineering tools while also engaging in a microbiome…
Read More A Brief History of CRISPR-Cas9 Genome-Editing Tools
Learn how the CRISPR prokaryote immune response systems were first discovered and the development of the CRISPR-Cas9 gene-editing tool.
Read More Level Up Your Drug Screening With CRISPR
Efficient Generation of Gene-edited Mouse Models and Cell Lines Using Synthetic sgRNA
Efficient Generation of Gene-edited Mouse Models and Cell Lines Using Synthetic sgRNA On Demand Webinar Dr. Peter Romanienko Managing Director, Genome Editing Shared Resource, Rutgers-Cancer Institute of New Jersey Dr. Peter Romanienko obtained his PhD training from Cornell Graduate School of Medical Sciences/Sloan Kettering Institute working on DNA repair in Dr. Maria Jasin’s laboratory and…
Read More Why You Should Consider Adding CRISPRa and CRISPRi to Your Toolbox
Find out how CRISPR-mediated gene activation (CRISPRa) and repression (CRISPRi) works and why you should consider using them in addition to your CRISPR knockouts.
Read More How CRISPR Can Be Used to Detect Emerging Viral Pathogens
The threat of pandemic viruses can be mitigated by developing diagnostic tests that are rapidly implementable and field deployable. Two CRISPR-based strategies, DETECTR and SHERLOCK, have been developed recently that not only meet these criteria but offer unprecedented multiplexing capabilities. This article discusses the theoretical basis of proposed and currently available viral diagnostics for educational…
Read More A CRISPR Approach to Immuno-Oncology
T cells can be tricky to engineer with CRISPR. Find out the key considerations when editing these cells and how you can overcome any associated challenges.
Read More Plant vs. Virus: Comparative transcriptomic analysis of single TuMV-infected micro-dissected plant cells
Plant vs. Virus: Comparative transcriptomic analysis of single TuMV-infected micro-dissected plant cells In this tutorial, you will find: How studying the early events of virus infection in plants avoids the dilution effect and allows identification of the key molecular players involved in initial plant-virus interactions. How the MMI CellCut microdissection system allows dissection of the…
Read More How to Understand CRISPR Formats and Their Applications
Find out how modified variants of CRISPR nucleases provide gene editing with reduced off-target effects and can even control gene expression without altering the DNA sequence.
Read More How to Validate a CRISPR Experiment
Discover how to validate the success of your CRISPR gene editing experiment from determining successful delivery of CRISPR reagents into your cells to the confirmation of desired genetic and phenotype changes.
Read More CRISPR Gene Editing: Considerations and Getting Started
Discover how to get started with CRISPR gene editing in your experiments with our key considerations.
Read More Engineering Vero Cell Lines Using CRISPR to Increase Production of Viral Vaccines
Engineering Vero Cell Lines Using CRISPR to Increase Production of Viral Vaccines On Demand Webinar Dr. Benjamin Borgo Dr. Benjamin Borgo currently leads a team of forward-thinking product and service managers within Merck KGaA’s Genome Engineering and Modulation franchise. His first exposure to CRISPR-based genome editing was as a graduate student where he attempted to…
Read More 3′ mRNA-Seq is Transforming Gene Expression Analysis
3′ mRNA-seq is a NGS method to profile gene expression that is sensitive and straightforward but won’t blow your budget.
Read More Bioinformatics: It’s Not All About Genomics
Getting Started with NGS Bioinformatics
Bioinformatics and NGS go together like peanut butter and jelly. But if you’re just starting out with these techniques it can be daunting.
Read More WGS Workflow: From Sample Collection to Data Interpretation
The efficiency of whole genome sequencing (WGS) workflows has skyrocketed since its inception. Major leaps and minor tweaks in the WGS workflow have compounded over time resulting in radical reductions in processing time and the cost of sequencing whole genomes over the past decades. The complete sequencing of the first human genome, named the Human…
Read More The Recipe for Successful Whole Genome Sequencing
The success of whole genome sequencing (WGS) is shown in the quick and efficient scientific response to the 2011 outbreak of E. coli in Germany and France.1 German and French strains of E. coli were indistinguishable using standard tests. However, WGS analysis showed 2 single nucleotide polymorphisms (SNPs) in the German strains and 9 SNPs…
Read More CRISPR Nucleases: The Ultimate Guide To Selection
CRISPR nucleases continue to dominate the gene-editing landscape because they are powerful and easy-to-use gene-editing tools. Their rapid discovery and development, however, make choosing the right nuclease for your experiment difficult. This guide highlights the broad range of available CRISPR nucleases and distils the major factors you should consider when choosing one for your next…
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