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MicroRNAs are 18–22 nucleotides long, which is too short for conventional RT-qPCR primers to bind and too similar to their precursors for most detection methods to distinguish. Here’s how stem-loop RT-PCR solves both of these problems, including a practical walkthrough from primer design through to data normalization. Stem-loop RT-PCR uses a hairpin-shaped reverse transcription primer…
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