Yevgeniy Grigoryev

Yevgeniy Grigoryev
Yevgeniy has a PhD in Systems genomics, immunology, molecular biology from Scripps Research. He is currently a lecturer at the City College of New York and a freelance science writer specializing in communicating science to diverse audiences. My extensive background in scientific research, science writing and journalism allows me to translate various scientific topics into clear, concise, and accurate language that appeals to a general audience. As I science journalist with Nature Medicine, I honed my skills as an effective communicator and writer, interacting with many scientists across many different fields, including policymakers, clinicians, and technology experts. Moreover, my experience as a science writer with the BiteSizeBio online scientific community allows me to promote and encourage communication among different sectors of the scientific community and cultivate new relationships within the community.

Articles by Yevgeniy Grigoryev

Interview Techniques: Interview like a STAR

Interview Techniques: Interview like a STAR

During your time interviewing for different jobs, more likely than not you will encounter employers who conduct behavioral interviews. What is a behavioral-based interview, you may ask? Behavioral interviewing is supposed to uncover your past job-related behavior to predict how you will behave in the future. It is based on the assumption that your past…

Mycoplasma: The Hidden Anarchist of Cell Culture

It is the black death of cell culture. Scientists don’t dare utter its name and many a graduate student has fallen victim to its indiscriminate menace. These stealthy anarchists infiltrate quietly but deliberately until their numbers swell and then they attack in strength, overwhelming their victims before they can put up a fight! What is…

5 Ways to Really Screw Up Your RNA Prep

Unlike DNA, which can last for eons, RNA is a fragile and degradation-prone cousin. After working with RNA for a while, one becomes quite paranoid about handling RNA because even a single sneeze or drop of saliva can potentially affect your results. The reason is that there are enzymes called RNases that specifically target and…

Crush Like an Elephant, Soak Like the Rain: Old-School DNA Gel Extraction

In my previous article on DNA gel extraction, I explained how most commercially available DNA gel extraction kits work. However, there was a time before our society was blessed with these convenient marvels of technology and scientists had to summon the gods of “Crush and Soak”. This method has been proven for millennia, as people…

How DNA Gel Extraction Works

Isolating pure DNA is key to many downstream applications for molecular biologists.  Isolating large quantities of pure DNA used to be a laborious task.  But thanks to commercially available kits, older methods have been streamlined to allow efficient recovery of pure DNA. In this article, I will talk about a method called DNA gel extraction,…

Top Tricks for Isolation of miRNAs from Plasma and Serum

Top Tricks for Isolation of miRNAs from Plasma and Serum

MicroRNAs (miRNA) are short, non-coding RNAs involved in post-transcriptional silencing of gene expression. miRNAs can be associated with exosomes and can function as cancer-specific biomarkers. This, coupled with the fact that they are stable in plasma and serum makes them valuable diagnostic tools, as long as they can be reliably isolated from the serum and…

How Can A Single Mutation Affect Splicing Regulation?

How Can A Single Mutation Affect Splicing Regulation?

Alternative splicing is a highly orchestrated process that uses a multitude of regulatory mechanisms. Splicing specificity involves a precise interaction between cis- and trans-acting regulatory elements, and factors that disrupt these interactions can result in aberrant splicing. There are multiple ways in which mutations can affect splicing fidelity: A point mutation in the cis-acting splice…

How to Detect Alternative Splicing Variants

How to Detect Alternative Splicing Variants

Alternative splicing events often occur in a spatiotemporal manner, and some are regulated by alternative splicing regulators, with striking variation across tissue types and developmental stages. Alternative splicing events are often differentially regulated across tissues and during development, as well as among individuals and populations, suggesting that individual isoforms may serve specific spatial or temporal…

Principles and Mechanisms of Mammalian Cell Transfection

Principles and Mechanisms of Mammalian Cell Transfection

Mammalian cell transfection is a technique commonly used to express exogenous DNA or RNA in a host cell line (for example, for generating RNAi probes). There are many different ways to transfect mammalian cells, depending on the cell line characteristics, desired effect, and downstream applications. In this article, I will review the different methods of…

How to Pursue a Non-Research Career While in Graduate School

How to Pursue a Non-Research Career While in Graduate School

‘Would you tell me, please, which way I ought to go from here?’ ‘That depends a good deal on where you want to get to,’ said the Cat. ‘I don’t much care where,’ said Alice. ‘Then it doesn’t matter which way you go,’ said the Cat.                                    From Alice’s Adventures in Wonderland. According to a…

What Has Methylation Done For You Lately?

What Has Methylation Done For You Lately?

Epigenetics is the study of heritable changes in the phenotype of a cell or an organism that are not encoded by the genome (hence epi which means ‘above’ in Greek, and genetikos which means ‘origin’). In this article, we’ll discuss DNA methylation, a common epigenetic modification: what it is, how to detect it, and how…

How To Use Your Department To The Fullest

How To Use Your Department To The Fullest

“No one can whistle a symphony.  It takes a whole orchestra to play it.” – H.E. Luccock In my previous article on “Starting your PhD the right way”, I already mentioned the importance of using your department’s resources to your advantage. In this article, I will expand on how to use your department to the…

When Silence Speaks Volumes: Using RNAi to Investigate Gene Function

When Silence Speaks Volumes: Using RNAi to Investigate Gene Function

RNA interference (RNAi) may have originated as a defense mechanism to protect cells against foreign genes introduced by viruses. This concept has since been put to use to create a powerful experimental tool for investigating gene function in organisms. Small-interfering RNA (siRNA) libraries for investigating genome-wide function can be produced by chemical synthesis of probes…

Tango Therapy

Tango Therapy

“While I dance I can not judge, I can not hate, I can not separate myself from life. I can only be joyful and whole. This is why I dance.” – Hans Bos While in graduate school, I tried really hard to find an activity that would allow me to disassociate my body from my…

How DNA Microarrays are Built

How DNA Microarrays are Built

Previously I introduced the DNA microarray technology and described the principle behind it: hybridization between the nucleic acid sequence from the biological samples being examined and a synthetic probe immobilized and spatially arrayed on a solid surface, the microarray. In this article, I will explain how these probes are designed and positioned on the array. I…