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Protein Expression and Analysis

5 Easy Ways to Tell Salt and Protein Crystals Apart

How do you make sure that the crystals you have grown are of your target protein? Can you tell salt and protein crystals apart without using X-ray diffraction? Yes, you can. Read on to discover five easy ways to distinguish between salt and protein crystals.

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Choosing the Right Bacterial Strains for Your Lab

Whether you’re simply seeking competent E. coli for a transformation experiment or building a panel intended for a whole project, it’s vital that you pick the right strain for your experiment. Find out more in our complete guide to choosing bacterial strains.

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Measuring Beyond the Limitations of ELISAs

Measuring Beyond the Limitations of ELISAs Available On Demand Speaker Craig Lanio Field Application ScientistMilliporeSigma Craig is a Field Application Scientist with MilliporeSigma and has been part of the Immunoassay Platform Solutions team for almost 15 years. Prior to coming to MilliporeSigma, he was a scientist with multiple biopharma companies where he participated in every…

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DIY Electrocompetent E. coli

Making good quality electrocompetent E. coli is very easy. One morning’s work (with a bit of work ahead of time) is all it takes.

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How SDS-PAGE Works

Knowing how SDS-PAGE works means that you can troubleshoot any issues in your experiment and tweak the setup to get publication-worthy figures. Find out how it works here.

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Getting Started with Immunohistochemistry

Immunohistochemistry isn’t just a useful clinical tool, it also has great applications as a basic research tool. We’ll walk you through the immunohistochemistry basics to get you off to a flying start.

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IL-13 is a Pathogenic Driver of COVID-19

IL-13 is a Pathogenic Driver of COVID-19 Available On-Demand Speaker Alexandra Donlan Graduate StudentUniversity of Virginia Alexandra received her bachelor’s degree in Biological Sciences from Florida State University, where she studied the role of exosomes in the oncogenesis of Epstein–Barr virus in the lab of Dr David Meckes. She is working towards her PhD at…

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Development of ultrasensitive immunoassays for SARS-CoV-2 antibody detection

Development of ultrasensitive immunoassays for SARS-CoV-2 antibody detection Available On Demand Speaker Rosemary Sweetwood Manager, CAST Sample Testing, Scientist II CAST Custom Assay DevelopmentMilliporeSigma Rosemary Sweetwood has worked with SMC™ technology for over 7 years in diagnostic development, assay development, and custom manufacturing, as well as managing clinical sample testing projects for our SMC™ Sample…

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IgG Antibodies Against SARS-CoV-2 Correlate to Days from Symptom Onset in COVID-19 Positive Patients

IgG Antibodies Against SARS-CoV-2 Correlate to Days from Symptom Onset in COVID-19 Positive Patients Available On Demand Speaker Mary Young Research TechnicianUniversity of Virginia, School of Medicine Mary Young has her Bachelors of Science in Biology from Elon University. She joined the Petri Lab 3 years ago as a research technician helping with several projects,…

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A-Z of Post-Translational Modifications

You might know the most common post-translational modifications, but there are many more than just phosphorylation and ubiquitination – come and test your knowledge!

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Assay Development for Quantification of Low-abundance Proteins Using Single Molecule Counting

Assay Development for Quantification of Low-abundance Proteins Using Single Molecule Counting On-Demand Sponsored by Sarah HamrenMilliporeSigma In this tutorial you will find: How MilliporeSigma’s Single Molecule Counting (SMC™) platform can be used to detect even very low levels of Huntingtin protein How to reach fM levels of sensitivity detecting both mHTT and total HTT Development…

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Icky Sticky: Heat Shock Protein Contamination during Protein Purification

Purifying a new protein is no easy feat. Finding combinations of protein purification buffer, salt, detergent, and stabilizing agent to get high yields of squeaky-clean protein can become tedious. Few things are as bothersome during this process as Heat Shock Protein (HSP) contamination. But worry not, we’ve got some handy tips to avoid HSP contamination…

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Spinning Around: Tips and Tricks for Using Centrifugal Filters

One of the most widespread protein laboratory accessories are the MWCO (molecular weight cut-off) centrifugal filters which are commonly used for concentrating protein, as well as DNA. They are available commercially with different cut-offs including 3kDa, 30kDa, 50kDa, 100kDa, and so on. These little devices are expensive and hence demand proper usage and care to…

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Designing a LOV2 Domain-Based Toolkit

Cell Biology is entering the Age of Light with a spectrum of new optogenetics tools available to control protein function using light. Once the remit of neuroscientists [1], the past decade has yielded a bounty of novel light-controllable domains that are now being leveraged to illuminate the dark corners of basic cell biology [2,3]. The…

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So You Think You Can PEMSA? A Guide to Protein Electrophoretic Mobility Shift Assay

Studying nucleic acid interactions with proteins can be accomplished using a rapid and efficient electrophoretic mobility shift assay (EMSA). This method is essentially an agarose gel electrophoresis technique that detects protein:nucleic acid interactions, as the mobility of the labeled nucleic acid will be retarded if bound to a protein (compared to unbound DNA). A lesser-known…

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The Mysterious World of Macrophages: How to Harness Them for Your Research

The Mysterious World of Macrophages: How to Harness Them for Your Research In this tutorial, you will learn: How to work with mouse and human macrophages The best methods for culturing macrophages The difference between M1 and M2 macrophages, and how to differentiate each population from monocytes What data to expect based on the conditions…

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How it Works: Storage Phosphor Screen

Radioactivity is still the most sensitive detection mechanism for many macromolecules and enzymatic activities. In graduate school, I performed countless radioactive kinase assays, watching the radioactive gamma 32P of ATP get transferred to my autophosphorylating receptor of interest, and then separating my protein from free hot ATP on a gel. The gel is dried, covered…

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An Exploration of the Sigmoidal Curve – Math for the Rest of Us

ELISA (Enzyme-Linked Immunosorbent Assay) is the heartbeat of many labs in the research world, owing to its simplicity and its ability to answer a very basic question: how much of protein/peptide/antibody is in my sample?  More specifically, it can be used to answer such questions as: How much IgG is in the serum after I…

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Will the Real NP-40 Please Stand Up? Chemical Nomenclature Woes.

One day, a colleague stopped by my workbench to ask which detergent would not break the nuclear membrane. Based on my previous experience using gentle detergents in lysis buffers, I replied, “NP-40”. However, we had two brands of NP-40. A closer look at the datasheets revealed that the chemical names were different even though they…

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How Protein Adhesion Can Affect Your Experiments and What to Do About It

Commonly, no one thinks much about how the surfaces of labware (like microcentrifuge tubes, test tubes, dishes, etc.) can affect experimental results. We might know when we need to use glass versus plastic. Or we might know that certain chemicals, like chloroform, will interact with some plastic polymers, and you must use polymers that are…

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Five Methods for Assessing Protein Purity and Quality

If you’ve ever worked with proteins in the lab, you probably know just how critical protein purity and quality are for downstream applications. In this article, we’ll review the multitude of problems that are encountered with ‘bad’ protein samples and how you can analyze the purity and integrity of your favorite protein prior to using…

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Let’s Come Clean: Why Quality Control for Purified Protein Matters

If you are a Michelin star chef, then your first priority for preparing your signature dish is to use the best ingredients. One rotten potato or one slightly overripe strawberry could ruin not just a dish, but also your reputation. In the laboratory we are (mostly) not cooking rotten potatoes, but we are doing delicate…

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8 Tricks to Improve Your Negative Staining of Membrane Proteins

Negative staining of proteins is a versatile tool for structural biology. The sample preparation protocol is simple: the sample is embedded in a heavy metal stain that gives rise to increased specimen contrast. Thus, negative staining is a very convenient method to assess sample homogeneity, formation of macromolecular complexes, or quality of protein preparation. Conventional…

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