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Glucose repression, leaky expression, and arabinose catabolism can all hinder protein expression. This short guide introduces the pBAD promoter and gives you some advice on using it for consistent protein yields for your studies.
Protein crystals are crucial for structure solution via X-ray crystallography but are notoriously hard to grow. We’ve got you covered with 5 clever protein crystallization seeding methods to grow impressive protein crystals.
Working with membrane proteins can be tricky. Read on to get tried-and-tested tips and tricks for expressing, extracting, and purifying these water-phobic proteins.
Fat and blurry bands on your SDS-PAGE gel can leave your experimental question unanswered. Learn how you can sharpen up your bands and get much greater resolution by using Bis-Tris gels.
Discover how digital ELISAs revolutionize protein detection with unparalleled sensitivity. This article explores cutting-edge technologies like Simoa that detect multiple biomarkers from small samples, transforming research in areas like neurology and immunology.
Metabolic pathways include all the chemical reactions in an organism that sustain biological function, and metabolic tracing lets us study them. This article explains what metabolic tracing is, the isotopes used for different types of study, and how to incorporate isotopes into samples.
Isoelectric focusing is a way of separating proteins and peptides based on their isoelectric point. Read all about this method and its applications right here.
In-cell Westerns are a powerful technique that has enhanced how researchers analyze protein expression levels and signaling pathways within fixed cells. Learn about their primary advantages, applications, and some of the best tech and products to perform them.
Purified proteins must be kept soluble and active. Discover how to design the perfect protein purification buffer—from additives you can include and their appropriate concentrations to important considerations.
Dive into the world of protein structure science, where AI is revolutionizing the field by offering accurate predictions and free, open-access tools. In this beginner’s guide, we’ll explore these groundbreaking tools, discuss their strengths and weaknesses, and examine the implications for traditional experimental methods like NMR and X-ray crystallography.
Here’s your reliable and adaptable nuclear extraction protocol. It contains buffer recipes, explanations of what the reagents do, and top tips to boost yields.
Confused about yeast protein extraction methods? Let this article guide you through the process and help you select the most appropriate method based on your research goals and available resources.
What is protein crystallography? Whether you are about to enter the world of protein crystallography or work with a protein crystallographer on your grant, this article will be useful.
Discover how Ponceau S works, how to perform staining, and get a detailed guide to interpreting what the staining means when troubleshooting your failed blots.
ECL is an expensive reagent. Why not learn how to make ECL yourself? This cheap and simple option will give you better blots more often!
Are you struggling with ugly and unreadable western blots? Here are 3 ways to optimize your western blot transfer and get blots to be proud of.
Western blot quantification can show how protein expression changes between conditions and treatments. Discover the 4 critical steps you need for quantification.
When working with proteins, accurate protein quantification is key for getting reliable data. Here are the top 5 quantification assays.
Discover what phosphorylation is, understand why you might want to mimic it, and learn 3 ways to mimic phosphorylation for both in vitro and in vivo applications
Getting to know your protein’s structure can help uncover deeper insights and inspire new hypotheses. Discover how protein data bank files can help.
Why is SDS-PAGE run vertically? Agarose gel electrophoresis is run horizontally, so why not SDS-PAGE? Here are 3 great answers.
Circular dichroism is a type of spectroscopy that can tell you the type and percentage of secondary structure units in a protein sample. Here are 5 handy pointers on best circular dichroism practice to set up your experiment properly.
Circular dichroism is a type of spectroscopy that can tell you the type and percentage of secondary structure units in a protein sample. This article will take you through circular dichroism theory and some of its applications.
SDS-PAGE is the technique that unites life scientists. We all perform it during our research to separate protein analytes and, therefore, we all need a good SDS-PAGE gel recipe.
Expressing your protein of interest but not sure if it’s properly folded or struggling with inclusion bodies? Read on to discover advice and tips for battling inclusion bodies and refolding proteins
There are several great protein staining methods, but how do you pick the one that’s appropriate to your intended application? Read on to find out.
The interaction of proteins with ligands is an important phenomenon. Learn how to detect these interactions (and much more) using the thermal shift assay. In this article, we’ve got you covered with the basic theory and an easy protocol too.
Protease inhibitors are a requirement in many lab experiments. In this article, we’ll take you through how protease inhibitors work, why we need them, and how to use them correctly and safely.
Homology modeling is a powerful tool that enables you to predict protein structures. But how do you do it? Read on to find out.
Laemmli buffer is used almost every day by life since researchers. But what do all those components actually do? Find out in this handy article
How do you make sure that the crystals you have grown are of your target protein? Can you tell salt and protein crystals apart without using X-ray diffraction? Yes, you can. Read on to discover five easy ways to distinguish between salt and protein crystals.
Whether you’re simply seeking competent E. coli for a transformation experiment or building a panel intended for a whole project, it’s vital that you pick the right strain for your experiment. Find out more in our complete guide to choosing bacterial strains.
Making good quality electrocompetent E. coli is very easy. One morning’s work (with a bit of work ahead of time) is all it takes.
Want to know not only if your proteins bind but quantify the interaction? We walk you through how to perform a quantitative yeast two-hybrid assay.
Excess detergent in your membrane proteins samples can cause you serious headaches. We’ve got you covered with 6 ways you can remove excess detergent from membrane proteins.
Knowing how SDS-PAGE works means that you can troubleshoot any issues in your experiment and tweak the setup to get publication-worthy figures. Find out how it works here.
Immunohistochemistry isn’t just a useful clinical tool, it also has great applications as a basic research tool. We’ll walk you through the immunohistochemistry basics to get you off to a flying start.
It’s not always easy deciding whether to run electrophoresis at a constant voltage, current, or power. Here, we outline the differences to help you make an informed decision.
Deciding on the right peptide sequence can make or break your experiment. Find out what to keep in mind when designing a synthetic peptide.
A quick start guide to methods of assessing protein post-translational modifications
The eBook with top tips from our Researcher community.