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Protein Expression and Analysis

The Essential Guide to In-Cell Westerns

In-cell Westerns are a powerful technique that has enhanced how researchers analyze protein expression levels and signaling pathways within fixed cells. Learn about their primary advantages, applications, and some of the best tech and products to perform them.

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A Beginner’s Guide to Protein Structure Prediction

Dive into the world of protein structure science, where AI is revolutionizing the field by offering accurate predictions and free, open-access tools. In this beginner’s guide, we’ll explore these groundbreaking tools, discuss their strengths and weaknesses, and examine the implications for traditional experimental methods like NMR and X-ray crystallography.

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Meltdown: A Hot Introduction to the Thermal Shift Assay

The interaction of proteins with ligands is an important phenomenon. Learn how to detect these interactions (and much more) using the thermal shift assay. In this article, we’ve got you covered with the basic theory and an easy protocol too.

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5 Easy Ways to Tell Salt and Protein Crystals Apart

How do you make sure that the crystals you have grown are of your target protein? Can you tell salt and protein crystals apart without using X-ray diffraction? Yes, you can. Read on to discover five easy ways to distinguish between salt and protein crystals.

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Choosing the Right Bacterial Strains for Your Lab

Whether you’re simply seeking competent E. coli for a transformation experiment or building a panel intended for a whole project, it’s vital that you pick the right strain for your experiment. Find out more in our complete guide to choosing bacterial strains.

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DIY Electrocompetent E. coli

Making good quality electrocompetent E. coli is very easy. One morning’s work (with a bit of work ahead of time) is all it takes.

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How SDS-PAGE Works

Knowing how SDS-PAGE works means that you can troubleshoot any issues in your experiment and tweak the setup to get publication-worthy figures. Find out how it works here.

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Getting Started with Immunohistochemistry

Immunohistochemistry isn’t just a useful clinical tool, it also has great applications as a basic research tool. We’ll walk you through the immunohistochemistry basics to get you off to a flying start.

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