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Protein Expression and Analysis

Development of ultrasensitive immunoassays for SARS-CoV-2 antibody detection

Development of ultrasensitive immunoassays for SARS-CoV-2 antibody detection Date: Tuesday 23rd February 2021Time: 8am Los Angeles, 10am Chicago, 11am New York, 4pm London, 5pm Berlin Speaker Rosemary Sweetwood Manager, CAST Sample Testing, Scientist II CAST Custom Assay DevelopmentMilliporeSigma Rosemary Sweetwood has worked with SMC™ technology for over 7 years in diagnostic development, assay development, and…

Image of SARS-CoV-2 virus to represent immunoassays for SARS-CoV-2Read More

IgG Antibodies Against SARS-CoV-2 Correlate to Days from Symptom Onset in COVID-19 Positive Patients

IgG Antibodies Against SARS-CoV-2 Correlate to Days from Symptom Onset in COVID-19 Positive Patients Date: Thursday 28th January 2021Time: 8am Los Angeles, 10am Chicago, 11am New York, 4pm London, 5pm Berlin Speaker Mary Young Research TechnicianUniversity of Virginia, School of Medicine Mary Young has her Bachelors of Science in Biology from Elon University. She joined the…

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A-Z of Post-Translational Modifications

You might know the most common post-translational modifications, but there are many more than just phosphorylation and ubiquitination – come and test your knowledge!

Image of alphabet, depicting the A-Z of post-translational modificationsRead More

Assay Development for Quantification of Low-abundance Proteins Using Single Molecule Counting

Assay Development for Quantification of Low-abundance Proteins Using Single Molecule Counting On-Demand Sponsored by Sarah HamrenMilliporeSigma In this tutorial you will find: How MilliporeSigma’s Single Molecule Counting (SMC™) platform can be used to detect even very low levels of Huntingtin protein How to reach fM levels of sensitivity detecting both mHTT and total HTT Development…

Neurons, showing how Single Molecule Counting can be used for detection of Huntingtin protein for Huntington’s diseaseRead More

Icky Sticky: Heat Shock Protein Contamination during Protein Purification

Purifying a new protein is no easy feat. Finding combinations of protein purification buffer, salt, detergent, and stabilizing agent to get high yields of squeaky-clean protein can become tedious. Few things are as bothersome during this process as Heat Shock Protein (HSP) contamination. But worry not, we’ve got some handy tips to avoid HSP contamination…

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Spinning Around: Tips and Tricks for Using Centrifugal Filters

One of the most widespread protein laboratory accessories are the MWCO (molecular weight cut-off) centrifugal filters which are commonly used for concentrating protein, as well as DNA. They are available commercially with different cut-offs including 3kDa, 30kDa, 50kDa, 100kDa, and so on. These little devices are expensive and hence demand proper usage and care to…

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Laemmli Buffer: What Is It for Anyway?

Electrophoresis encompasses a wide range of techniques in which charged biomolecules in a liquid, a solid, or a semisolid solution can be separated by size under the application of an electric field. The most common application of electrophoresis for the separation of proteins is SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis), which has been previously covered here.  …

Caricamento di un gel di agarosioRead More

Designing a LOV2 Domain-Based Toolkit

Cell Biology is entering the Age of Light with a spectrum of new optogenetics tools available to control protein function using light. Once the remit of neuroscientists [1], the past decade has yielded a bounty of novel light-controllable domains that are now being leveraged to illuminate the dark corners of basic cell biology [2,3]. The…

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So You Think You Can PEMSA? A Guide to Protein Electrophoretic Mobility Shift Assay

Studying nucleic acid interactions with proteins can be accomplished using a rapid and efficient electrophoretic mobility shift assay (EMSA). This method is essentially an agarose gel electrophoresis technique that detects protein:nucleic acid interactions, as the mobility of the labeled nucleic acid will be retarded if bound to a protein (compared to unbound DNA). A lesser-known…

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The Mysterious World of Macrophages: How to Harness Them for Your Research

The Mysterious World of Macrophages: How to Harness Them for Your Research In this tutorial, you will learn: How to work with mouse and human macrophages The best methods for culturing macrophages The difference between M1 and M2 macrophages, and how to differentiate each population from monocytes What data to expect based on the conditions…

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How it Works: Storage Phosphor Screen

Radioactivity is still the most sensitive detection mechanism for many macromolecules and enzymatic activities. In graduate school, I performed countless radioactive kinase assays, watching the radioactive gamma 32P of ATP get transferred to my autophosphorylating receptor of interest, and then separating my protein from free hot ATP on a gel. The gel is dried, covered…

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