Protein Expression and Analysis
Top 5 Protein Quantification Assays
When working with proteins, accurate protein quantification is key for getting reliable data. Here are the top 5 quantification assays.
Read MoremiRNA as a Novel Toxicity Biomarker
miRNA as a Novel Toxicity Biomarker Date: Tuesday July 26th 2022Time: 8am Los Angeles, 10am Chicago, 11am New York, 4pm London, 5pm Berlin Speakers Juan J. Díaz-Mochón, Ph.D. Chief Executive Officer, DESTINA Genomics Ltd Anitaben Tailor, Ph.D. Biology Technical Marketing Manager, Merck KGaA, Darmstadt, Germany After earning a PhD in Medicinal Chemistry at the Universidad de…
Read MoreImmunoassay Verification and Quality Control Practices and How They Affect Data Outputs
Immunoassay Verification and Quality Control Practices and How They Affect Data Outputs Available On Demand Speaker Geoffrey J. Solares, Ph.D. Immunoassay Field Application Scientist – Southwest Region Dr. Solares first began immunoassay work in 2006 by mastering western blot techniques as an undergrad and continued developing techniques during his MSc at California State University Northridge…
Read More3 Easy Ways to Mimic Phosphorylation
Discover what phosphorylation is, understand why you might want to mimic it, and learn 3 ways to mimic phosphorylation for both in vitro and in vivo applications
Read MoreProtein Structure Analysis: Extra Information in a Protein Data Bank File
Getting to know your protein’s structure can help uncover deeper insights and inspire new hypotheses. Discover how protein data bank files can help.
Read MoreWhy Is SDS-PAGE Run Vertically? Here are 3 Great Answers
Why is SDS-PAGE run vertically? Agarose gel electrophoresis is run horizontally, so why not SDS-PAGE? Here are 3 great answers.
Read MoreAn Introduction to Circular Dichroism Part 2: Circular Dichroism Practice
Circular dichroism is a type of spectroscopy that can tell you the type and percentage of secondary structure units in a protein sample. Here are 5 handy pointers on best circular dichroism practice to set up your experiment properly.
Read MoreAn Introduction to Circular Dichroism Part 1: Circular Dichroism Theory
Circular dichroism is a type of spectroscopy that can tell you the type and percentage of secondary structure units in a protein sample. This article will take you through circular dichroism theory and some of its applications.
Read MoreA Simple SDS-PAGE Gel Recipe and 10-Step Casting Protocol for Perfect Gels
SDS-PAGE is the technique that unites life scientists. We all perform it during our research to separate protein analytes and, therefore, we all need a good SDS-PAGE gel recipe.
Read More5 Protein Crystallization Seeding Techniques for Bigger and Better Crystals
Protein crystals are crucial for structure solution via X-ray crystallography but are notoriously hard to grow. We’ve got you covered with 5 clever protein crystallization seeding methods to grow impressive protein crystals.
Read MoreA Protein Renaturation Toolkit: 21 Tips and Tricks for Refolding Proteins
Expressing your protein of interest but not sure if it’s properly folded or struggling with inclusion bodies? Read on to discover advice and tips for battling inclusion bodies and refolding proteins
Read MoreProtein Staining Methods: An Overview of 3 of the Best
There are several great protein staining methods, but how do you pick the one that’s appropriate to your intended application? Read on to find out.
Read MoreMeltdown: A Hot Introduction to the Thermal Shift Assay
The interaction of proteins with ligands is an important phenomenon. Learn how to detect these interactions (and much more) using the thermal shift assay. In this article, we’ve got you covered with the basic theory and an easy protocol too.
Read MoreProtease Inhibitors 101: How They Work and How to Use Them
Protease inhibitors are a requirement in many lab experiments. In this article, we’ll take you through how protease inhibitors work, why we need them, and how to use them correctly and safely.
Read MoreHomology Modeling in the World of Proteins
Homology modeling is a powerful tool that enables you to predict protein structures. But how do you do it? Read on to find out.
Read MoreLaemmli Buffer: What Is It for Anyway?
Laemmli buffer is used almost every day by life since researchers. But what do all those components actually do? Find out in this handy article
Read MoreIsoelectric Focusing: A Simple Way to Enhance Your Protein Separation
Isoelectric focusing is a way of separating proteins and peptides based on their isoelectric point. Read all about this method and its applications right here.
Read More5 Easy Ways to Tell Salt and Protein Crystals Apart
How do you make sure that the crystals you have grown are of your target protein? Can you tell salt and protein crystals apart without using X-ray diffraction? Yes, you can. Read on to discover five easy ways to distinguish between salt and protein crystals.
Read MoreChoosing the Right Bacterial Strains for Your Lab
Whether you’re simply seeking competent E. coli for a transformation experiment or building a panel intended for a whole project, it’s vital that you pick the right strain for your experiment. Find out more in our complete guide to choosing bacterial strains.
Read MoreMeasuring Beyond the Limitations of ELISAs
Measuring Beyond the Limitations of ELISAs Available On Demand Speaker Craig Lanio Field Application ScientistMilliporeSigma Craig is a Field Application Scientist with MilliporeSigma and has been part of the Immunoassay Platform Solutions team for almost 15 years. Prior to coming to MilliporeSigma, he was a scientist with multiple biopharma companies where he participated in every…
Read MoreDIY Electrocompetent E. coli
Making good quality electrocompetent E. coli is very easy. One morning’s work (with a bit of work ahead of time) is all it takes.
Read MoreA Step-by-Step Guide to the Quantitative Beta-galactosidase Assay for Y2H Screening
Want to know not only if your proteins bind but quantify the interaction? We walk you through how to perform a quantitative yeast two-hybrid assay.
Read MoreBeat Back the Bubbles: 6 Ways to Remove Excess Detergent from Your Membrane Protein Sample
Excess detergent in your membrane proteins samples can cause you serious headaches. We’ve got you covered with 6 ways you can remove excess detergent from membrane proteins.
Read MoreHow SDS-PAGE Works
Knowing how SDS-PAGE works means that you can troubleshoot any issues in your experiment and tweak the setup to get publication-worthy figures. Find out how it works here.
Read MoreGetting Started with Immunohistochemistry
Immunohistochemistry isn’t just a useful clinical tool, it also has great applications as a basic research tool. We’ll walk you through the immunohistochemistry basics to get you off to a flying start.
Read MoreWorking with Membrane Proteins: Tips for Expressing, Extracting, and Purifying Your Membrane Protein Sample
Working with membrane proteins can be tricky. Read on to get tried-and-tested tips and tricks for expressing, extracting, and purifying these water-phobic proteins.
Read MoreConstant Current or Voltage in SDS-PAGE: The Great Debate
It’s not always easy deciding whether to run electrophoresis at a constant voltage, current, or power. Here, we outline the differences to help you make an informed decision.
Read MoreIL-13 is a Pathogenic Driver of COVID-19
IL-13 is a Pathogenic Driver of COVID-19 Available On-Demand Speaker Alexandra Donlan Graduate StudentUniversity of Virginia Alexandra received her bachelor’s degree in Biological Sciences from Florida State University, where she studied the role of exosomes in the oncogenesis of Epstein–Barr virus in the lab of Dr David Meckes. She is working towards her PhD at…
Read MoreDevelopment of ultrasensitive immunoassays for SARS-CoV-2 antibody detection
Development of ultrasensitive immunoassays for SARS-CoV-2 antibody detection Available On Demand Speaker Rosemary Sweetwood Manager, CAST Sample Testing, Scientist II CAST Custom Assay DevelopmentMilliporeSigma Rosemary Sweetwood has worked with SMC™ technology for over 7 years in diagnostic development, assay development, and custom manufacturing, as well as managing clinical sample testing projects for our SMC™ Sample…
Read MoreIgG Antibodies Against SARS-CoV-2 Correlate to Days from Symptom Onset in COVID-19 Positive Patients
IgG Antibodies Against SARS-CoV-2 Correlate to Days from Symptom Onset in COVID-19 Positive Patients Available On Demand Speaker Mary Young Research TechnicianUniversity of Virginia, School of Medicine Mary Young has her Bachelors of Science in Biology from Elon University. She joined the Petri Lab 3 years ago as a research technician helping with several projects,…
Read MoreSynthetic Peptides Part 2: Tips and Tricks for Peptide Synthesis
Deciding on the right peptide sequence can make or break your experiment. Find out what to keep in mind when designing a synthetic peptide.
Read MoreDetecting Post-Translational Modifications
A quick start guide to methods of assessing protein post-translational modifications
Read MoreA-Z of Post-Translational Modifications
You might know the most common post-translational modifications, but there are many more than just phosphorylation and ubiquitination – come and test your knowledge!
Read MoreSynthetic Peptides and Their Uses: Part 1
Are you studying a small peptide or protein? Learn whether using synthetic peptides can save you hours of transfection, protein expression, and purification.
Read MoreAn Experimental Toolkit for Measuring Autophagic Flux
How good is your cell at recycling? In this guide, we explain what autophagic flux is, why you need to study it, and methods to get you started.
Read MoreTop 5 Things to Keep in Mind When Detecting Non-Histone Lysine Acetylation
Lysine acetylation isn’t just for histones. Read on to find out how to determine whether your favorite protein is lysine acetylated.
Read MoreRecombinant Protein Expression: How to Choose the Right Affinity Tag
Using the right affinity tag can make protein purification a breeze. Read more to find out which tag is right for you.
Read MoreImmune Responses in Severe COVID-19 Patients
Speaker Professor Dexi Chen, the Director of the Beijing Institute of Hepatology, present data on immune responses in severe COVID-19.
Read MoreA Guide to Gradient Gels: The Why’s and How’s
Fret no more over fuzzy bands! We uncover how gradient gels can give you better results, and maximize your precious samples when performing SDS-PAGE
Read MoreAssay Development for Quantification of Low-abundance Proteins Using Single Molecule Counting
Assay Development for Quantification of Low-abundance Proteins Using Single Molecule Counting On-Demand Sponsored by Sarah HamrenMilliporeSigma In this tutorial you will find: How MilliporeSigma’s Single Molecule Counting (SMC™) platform can be used to detect even very low levels of Huntingtin protein How to reach fM levels of sensitivity detecting both mHTT and total HTT Development…
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