Protein Expression and Analysis
Development of ultrasensitive immunoassays for SARS-CoV-2 antibody detection
Development of ultrasensitive immunoassays for SARS-CoV-2 antibody detection Date: Tuesday 23rd February 2021Time: 8am Los Angeles, 10am Chicago, 11am New York, 4pm London, 5pm Berlin Speaker Rosemary Sweetwood Manager, CAST Sample Testing, Scientist II CAST Custom Assay DevelopmentMilliporeSigma Rosemary Sweetwood has worked with SMC™ technology for over 7 years in diagnostic development, assay development, and…
Read More IgG Antibodies Against SARS-CoV-2 Correlate to Days from Symptom Onset in COVID-19 Positive Patients
IgG Antibodies Against SARS-CoV-2 Correlate to Days from Symptom Onset in COVID-19 Positive Patients Date: Thursday 28th January 2021Time: 8am Los Angeles, 10am Chicago, 11am New York, 4pm London, 5pm Berlin Speaker Mary Young Research TechnicianUniversity of Virginia, School of Medicine Mary Young has her Bachelors of Science in Biology from Elon University. She joined the…
Read More Detecting Post-Translational Modifications
A-Z of Post-Translational Modifications
You might know the most common post-translational modifications, but there are many more than just phosphorylation and ubiquitination – come and test your knowledge!
Read More Synthetic Peptides and Their Uses: Part 1
Are you studying a small peptide or protein? Learn whether using synthetic peptides can save you hours of transfection, protein expression, and purification.
Read More An Experimental Toolkit for Measuring Autophagic Flux
How good is your cell at recycling? In this guide, we explain what autophagic flux is, why you need to study it, and methods to get you started.
Read More Top 5 Things to Keep in Mind When Detecting Non-Histone Lysine Acetylation
Lysine acetylation isn’t just for histones. Read on to find out how to determine whether your favorite protein is lysine acetylated.
Read More Recombinant Protein Expression: How to Choose the Right Affinity Tag
Using the right affinity tag can make protein purification a breeze. Read more to find out which tag is right for you.
Read More Immune Responses in Severe COVID-19 Patients
Speaker Professor Dexi Chen, the Director of the Beijing Institute of Hepatology, present data on immune responses in severe COVID-19.
Read More A Guide to Gradient Gels: The Why’s and How’s
Fret no more over fuzzy bands! We uncover how gradient gels can give you better results, and maximize your precious samples when performing SDS-PAGE
Read More Assay Development for Quantification of Low-abundance Proteins Using Single Molecule Counting
Assay Development for Quantification of Low-abundance Proteins Using Single Molecule Counting On-Demand Sponsored by Sarah HamrenMilliporeSigma In this tutorial you will find: How MilliporeSigma’s Single Molecule Counting (SMC™) platform can be used to detect even very low levels of Huntingtin protein How to reach fM levels of sensitivity detecting both mHTT and total HTT Development…
Read More Biology Beyond the Cell: the How and Why of Cell-Free Systems
Are your cell membranes more of a hindrance than a help? Struggling to sneak your DNA inside? Why not break out and take your expression or metabolism studies cell-free. Find out the what, how and why of cell-free systems.
Read More Fractionating Proteins by Solubility: or, How I Learned to Stop Worrying and Love the Ultracentrifuge
Does the term “ultracentrifuge” make your heart begin to race? Fear not! This article will provide you with tried and true tips on how to handle this piece of machinery like a boss.
Read More Icky Sticky: Heat Shock Protein Contamination during Protein Purification
Purifying a new protein is no easy feat. Finding combinations of protein purification buffer, salt, detergent, and stabilizing agent to get high yields of squeaky-clean protein can become tedious. Few things are as bothersome during this process as Heat Shock Protein (HSP) contamination. But worry not, we’ve got some handy tips to avoid HSP contamination…
Read More Spinning Around: Tips and Tricks for Using Centrifugal Filters
One of the most widespread protein laboratory accessories are the MWCO (molecular weight cut-off) centrifugal filters which are commonly used for concentrating protein, as well as DNA. They are available commercially with different cut-offs including 3kDa, 30kDa, 50kDa, 100kDa, and so on. These little devices are expensive and hence demand proper usage and care to…
Read More Laemmli Buffer: What Is It for Anyway?
Electrophoresis encompasses a wide range of techniques in which charged biomolecules in a liquid, a solid, or a semisolid solution can be separated by size under the application of an electric field. The most common application of electrophoresis for the separation of proteins is SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis), which has been previously covered here. …
Read More Designing a LOV2 Domain-Based Toolkit
Cell Biology is entering the Age of Light with a spectrum of new optogenetics tools available to control protein function using light. Once the remit of neuroscientists [1], the past decade has yielded a bounty of novel light-controllable domains that are now being leveraged to illuminate the dark corners of basic cell biology [2,3]. The…
Read More So You Think You Can PEMSA? A Guide to Protein Electrophoretic Mobility Shift Assay
Studying nucleic acid interactions with proteins can be accomplished using a rapid and efficient electrophoretic mobility shift assay (EMSA). This method is essentially an agarose gel electrophoresis technique that detects protein:nucleic acid interactions, as the mobility of the labeled nucleic acid will be retarded if bound to a protein (compared to unbound DNA). A lesser-known…
Read More The Mysterious World of Macrophages: How to Harness Them for Your Research
The Mysterious World of Macrophages: How to Harness Them for Your Research In this tutorial, you will learn: How to work with mouse and human macrophages The best methods for culturing macrophages The difference between M1 and M2 macrophages, and how to differentiate each population from monocytes What data to expect based on the conditions…
Read More How it Works: Storage Phosphor Screen
Radioactivity is still the most sensitive detection mechanism for many macromolecules and enzymatic activities. In graduate school, I performed countless radioactive kinase assays, watching the radioactive gamma 32P of ATP get transferred to my autophosphorylating receptor of interest, and then separating my protein from free hot ATP on a gel. The gel is dried, covered…
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