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The unique feature of real-time quantitative polymerase chain reaction (RT-qPCR) is that it associates the amplification of your target gene with a fluorescent signal in a quantifiable manner. Presently, there are numerous fluorescent tool kits/methods to consider when designing your RT-qPCR experiment. However, the two major categories to choose from are fluorescent intercalating dyes and…
When working very closely with a team of people in the lab, you will have disagreements leading to conflict. That’s just human nature, hence the origin of a common phrase, “Can’t we all just get along?” If you are lucky, you will be able to resolve the conflict. But then the question becomes, how will this…
The mere mention of thesis writing causes many students to instantly reach for their panic button. I was no different. I was freaking out about the mere thought of all that writing. However, after successfully preparing and defending my thesis, I learned that the process is just that, a process. Below are 5 points I…
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