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Every RT-qPCR experiment includes a detection chemistry decision: dsDNA-binding dye or fluorescent probe? The answer depends on your target number, multiplexing needs, assay stage, throughput, and budget (or whatever you can borrow from your lab colleagues for free!) Here’s a side-by-side comparison of SYBR Green and TaqMan with an interactive tool that recommends the right…
When working very closely with a team of people in the lab, you will have disagreements leading to conflict. That’s just human nature, hence the origin of a common phrase, “Can’t we all just get along?” If you are lucky, you will be able to resolve the conflict. But then the question becomes, how will this…
The mere mention of thesis writing causes many students to instantly reach for their panic button. I was no different. I was freaking out about the mere thought of all that writing. However, after successfully preparing and defending my thesis, I learned that the process is just that, a process. Below are 5 points I…
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