Balachandar

Bala is a graduate student in Germany. He loves technology and can often be found in the blogosphere and the interweb. His free time is consumed by photography and books.

Articles by Balachandar

Quick and Dirty Screening for Cloned Inserts

Quick and Dirty Screening for Cloned Inserts

For identifying positive clones from a plasmid cloning procedure, the routine of performing a mini-prep and then checking the putative clones by restriction digestion is most commonly used. Of course, if you need to screen a large number of clones, another option is a colony PCR to identify positives, followed by restriction digests to confirm….