Basic Lab Skills and Know-how
How To Calculate The Number Of Molecules In Any Piece of DNA
Messing up the initial calculations in your experiment is setting yourself up for failure. Here is a quick refresh on calculating the number of molecules in any DNA sample.
Read More 6 Laboratory Sterilization Methods
Effective laboratory sterilization methods are essential for working with isolated cell lines. Read our guide to the top 6 sterilization techniques to banish those bugs.
Read More Antibiotic Disposal in the Lab: Simple Tips to Get it Right
Have you ever given thought to how you are disposing of antibiotics in the lab and whether or not it’s correct? Discover the problems associated with improper antibiotic disposal and how to correctly dispose of different antibiotics in the lab.
Read More How to Reduce Your Use of Animal Products in the Lab
Did you know that there are many alternatives to the animal-derived products we use in the lab? Read more for how and why you might want to make the switch.
Read More Talking When Terrified: Tips for a Nervous Presenter
When I was in school, I absolutely hated giving talks. I was a really nervous presenter, my heart would start beating faster, my face would go red, my hands would shake…even my voice would tremble! Since then I’ve made some big breakthroughs, and now I absolutely love giving talks and lectures. Here are my top…
Read More How to Write an Effective Lab Protocol
We’ve all been there. You’re looking to replicate a result you have read in a paper, or maybe even one that has come from someone else in your own lab. But try as you might, you can’t get your head around the less than effective lab protocol that’s been provided. Or, worse still, you are…
Read More The How and Why of Limit of Detection
When developing an assay, whether it is for basic research or for use in diagnostics, you will often be asked about your assay’s sensitivity. This is perhaps one of the most important performance characteristics you can determine for an assay, and in regulated work, such as in vitro diagnostic (IVD) development and clinical diagnostics, it…
Read More To Sonicator and Beyond – Large Cell Volume Lysis Methods
At some point you have to leave small-scale cell lysis and move to large culture volumes for experiments currently in vogue, be it microarrays, total RNA libraries, or large-scale pull-downs for interactome or metabolome analysis. And at this point, you have to change your lysis method from an on-the-bench in eppendorfs to one capable of…
Read More Four Free and Easy-To-Use Online Primer Design Tools
Designing and running PCR reactions in the lab has become so commonplace that the number of primer design tools available can be a bit overwhelming for a beginner (or even an experienced molecular biologist!). Below are four of my favorite online programs available to make primer design quick, easy, and effective. A quick note before…
Read More 10 Steps to Enjoying Fieldwork for Sedentary Scientists
Note: Physically competent field scientists who find fieldwork a breeze may scoff at the suggestions here As a bench scientist whose only form of physical exercise in the laboratory is pipetting, I vividly remember my first fieldtrip to the wilderness. It was a trip to an island off the coast of Singapore to collect water…
Read More Ten Tips for Pipetting the 384-Well Plate
I was so excited to start using 384-well plates for my assays. With so many wells, these plates are useful for testing many conditions in parallel, as required in ELISAs, siRNA library screens, and drug treatment dilutions. However, I quickly learned that pipetting in these plates is more complicated than I thought. This article contains…
Read More Simple Tips for a Clean(-ish) Lab Drawer
Picture it: 6:00 pm on a Friday night. You have one or more experiments running. Maybe you’re doing a western blot, or following a staining protocol for an immunohistochemistry experiment, or just labeling tubes. But rather than working on active experiments, you’re helplessly searching through the lab drawer for that one pair of forceps, that…
Read More What Is Reproducible Research?
Once upon a time, I thought reproducible research meant if someone else showed X in a paper, then I should be able to get X in my experiment. However, this actually refers to replication, an important but separate concept. Reproducible research is data analysis that starts with the raw data and arrives at the same…
Read More What to Do During That Awkward One-Minute Spin
We’ve all been there. Twiddling our thumbs. Staring off into space. Pacing back and forth. This is the dreaded one-minute spin. If you’ve dabbled in molecular biology, you’ve likely encountered this awkward time. Not exactly enough time to actually do anything else, but when you’ve got nothing to do but wait, one minute seems like…
Read More Labeling For Life – Get a Good Self-Tracking Labeling System
When you work in a laboratory, preparing samples sets for many different experiments is a large part of the job. Keeping track of your samples can be tedious or even challenging if you don’t already have a good system in place. However, getting this right is a critical part of the experimental process. In this…
Read More Genetic Notation: Crack the Code!
Pop Quiz Time: You get a new bacterial strain from a culture collection, but you’re not quite sure what the genetic notation (i.e., all the letters and symbols) means. Do you: A. Cry? B. Ask around to see what your lab mates think? C. Cross your fingers that your friends at Bitesize Bio can help…
Read More Don’t Let Bubbles Burst Your Experimental Excitement
Bubbles isn’t just the name of my favorite cartoon character from Power Puff girls, or just the best activity for a kid to play with, in general. In my adult world, they stand for a whole lot more, but can still cause extreme emotions. At the lab bench, seeing bubbles brings happiness or sadness depending…
Read More Earn That Green Thumb! An Introduction to Working in a Greenhouse
If you have worked in a lab before, you probably think you are prepared to work anywhere. You’ve done the safety classes, know how to store the chemicals, even know how to work the chemical shower. Unfortunately, that doesn’t fully prepare you for greenhouse work. Greenhouses are a different kind of greenery-filled animal entirely, and…
Read More ChemDraw: a Versatile Molecule Sketching Tool for (Bio)Chemists
Have you ever wondered how to make professional, easy-to-understand figures of molecules for presentations or publications? While several programs exist for this purpose, ChemDraw is like the Swiss Army knife of chemical sketching programs that most chemists and journals use to prepare figures. Beyond the ability to create chemically accurate and legible figures, ChemDraw can…
Read More Water your choices? Understanding Types of Water in the Lab
If you are working in a scientific laboratory, it is very important to be aware of the various types of water available, because the purity may not be acceptable for your specific experimental application. In most labs, there are generally two types of water piped in to the sinks: Industrial Water Industrial water is non-potable…
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