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Nucleic acid extraction kits are routinely used in today’s molecular biology labs. Read on to learn more about what is inside these black boxes of wonder and how you can get the best results for your preps.
Most experiments start with a piece of DNA—either plasmid DNA or genomic DNA. And your downstream uses for it dictate how much you need, what contaminants you can tolerate, and your extraction and purification methods. In this article, we explain the key differences between plasmid and genomic DNA extraction methods.
Are your plasmid preps giving your poor yields? We’ve got 11 reasons that might be causing it and how to fix them.
Understanding how DNA extraction kits work is the key to troubleshooting your extraction issues.
Want to leave the lab but not sure what alternative career options are available? Our guide can help you decide what to do after hanging up your lab coat.
The lab is a melting-pot full of workers from different cultures and backgrounds, so some conflicts of personality are inevitable. However, when the lab head is the person that you are struggling to get along with, it can make your life a lot harder. Check out some different personality types and get advice on how to work with them effectively.
Formalin fixed paraffin embedded (FFPE) tissues are valuable samples that typically come from human specimens collected for examination of the histology of biopsies for the detection of cancer. But each sample contains much more information just waiting to be unlocked. Despite the tiny sample size, DNA can be extracted from the tissue sections and used…
Having trouble with your cloning? It might be to do with the DNA gel extraction. Get our top 10 DNA gel extraction tips to help you out.
Does your lab have a closet full of white elephants; once expensive instruments that are no longer fit for purpose, or have broken down? In many cases, all of that wasted money and resource could have been saved if the buyers had made smart choices about matching the instrument more closely to their needs. A…
RNA purification may be a common procedure in molecular biology but it is by far the one that people fear most. Why? Dreaded RNase. It’s everywhere… all over your bench and pipettes, and floating in the air, waiting for the chance to creep into your prep, shred your RNA into nucleotides, and ruin a day’s…
Bitesize Bio has had a lot to say about RNA isolation, mainly because it is one of the most anxiety-producing requirements for molecular biology; especially when you are first starting out (although isolating proteins from complex samples like soil and stool is far more difficult, let me tell you. But that’s a future post.) We’ve…
As widely used as it is, isolating RNA remains one of the more finicky protocols. Just about anyone who has performed the technique has their own personal tips and tricks to successfully isolate intact RNA from their samples with consistency. Although RNA can be somewhat unpredictable since it is so labile, there are a few…
The decision to make the switch from an academic lab and career to a biotech company doesn’t come easy. Many scientists are wary of the lack of independence and doing science for profit. But there are advantages too. Working in a biotech lab allows you to work on many varied and interesting projects that actually come to…
When I was in graduate school, it seemed that almost no one aspired to work in industry or be part of a company. But times are changing. Now, when I go to conferences and talk to scientists in training, I am asked, “how do I get a job in a company?” and “How did you get…
Northern and Southern blotting are now a thing of the past. They’ve been replaced with a faster and more quantitative technique. No longer do we wait days to know whether a gene is expressed. We can have the answer in 45 minutes! Real-time PCR is now commonly employed in almost all molecular biology laboratories to…
While reading my back issues of Applied and Environmental Microbiology (AEM), I came across an interesting paper that detailed an in-depth study on the effectiveness of hand cleaners to remove Norwalk virus (NV) from intentionally contaminated hands. Yes that’s right – intentionally contaminated, and how. The study volunteers allowed a 20% stool suspension containing Norwalk virus to be…
One of the very first things you need to do when getting set up for quantitative PCR (qPCR) is to determine the efficiency of the assay because knowing the assay efficiency is critical to accurate data interpretation. Here’s how.
A recent article published by The Scientist called Power Couples gave advice and examples for scientist couples who have successfully balanced their life at home and in the lab. It was interesting from the perspective of how two very busy and career motivated people work together to have it all: raise a family, run a lab, and stay in love…
We received the following question from Bitesize Bio reader, Beheroze Sattha. It relates to a problem with absolute quantification using plasmids for standard curves. Since many people use this technique it is an interesting one question for us to explore, and it also gives us a great opportunity to cover some important tips for performing…
The fourth Thursday of November marks the annual tradition in the U.S. called Thanksgiving. Originally, Thanksgiving was a religious holiday that has sinced turned secular and became a national holiday in 1941. Now, for families celebrating Thanskgiving, it is a time to cook a whole lot of food and eat way too much pumpkin pie….
In a previous article, I listed some ways that people annoy their co-workers and many of you added some of your own pet peeves. Now I would like to discuss some ways to be the lab favorite, also known as the “golden child”. Does your lab have a “golden child”? Someone who is always perfect,…
If you’re like many researchers, problems with PCR amplifying high GC DNA templates will be a major annoyance for you. Many strategies developed to overcome this issue. Betaine is the most common PCR additive used to enhance amplification of GC rich sequences because of its ability to dissolve secondary structure that blocks polymerase action. But…
qPCR is a technique used daily in most labs, but the first step, designing your qPCR primers, can be the biggest obstacle to even getting started. Without a good pair of primers, you can’t start asking the real questions and generating data. And sometimes the effort involved in optimizing an assay for high efficiency and sensitivity…
So you have some blood stored in the -20C or -80C and you want to isolate RNA from the samples. If you wanted DNA, you would have many products to choose from. But for RNA, your choices are more limited. Obtaining RNA From Frozen Blood is Difficult Why is that? The reason is that most RNA from…
If you ask any finished graduate student, most of us starting a Masters or PhD program were very excited at what awaited us and if you were anything like me, you were foolishly idealistic and thought you were going to pull on a lab coat, cure cancer and save the world. Ok, maybe most people…
Thanks to Bitesize Bio reader, Muthu Arumugam for contacting us about some problems he has been having with restriction digestion and clean up of DNA. I have boiled his query down to four main questions that are pertinent for most molecular biologists, so I hope that Muthu and everyone else can learn something from my…
It seems there is a reality TV show for virtually every type of person or profession. From Alaska king crab fishermen to surviving the outdoors to living the life of a privileged housewife, you name it and there is a show about it. So why not a show on the challenges and antics of people…
Gel extraction — what could be easier? Now we have quick and easy gel extraction kits, we no longer need to use time-consuming old fashioned methods like electro-elution or “freeze and squeeze”. Thank goodness. When Gel Extraction Goes Wrong But even the simplest of procedures can go wrong. Maybe you were distracted, confused, or thought…
The reverse transcription (RT) step of RT-PCR for converting RNA to cDNA is critical for accuracy in quantification and for finding low copy messages. Thus, you want to make sure that this step is performed with the highest efficiency but without having to optimize every single step. To help you further in optimizing the RT…
Removing unwanted DNA from vectors is one of the most routine laboratory techniques in cloning. Check out our top tips.
In my current position, I had the opportunity to hire a new R&D scientist to join the team. I was excited at being able to build my team and take my time to find the perfect fit for our company. My experience in the process of hiring gave me a new perspective on all the…
After all that hard work, you finally have your PhD. Now what? If your career choice is academic research, your first post-doc position beckons. The choice of where, and with whom, to take up a post-doc position is a very important one as it is at the post-doc stage where publications are required to move…
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