Suzanne Kennedy

Suzanne Kennedy
Suzanne has a PhD in Microbiology/Immunology from Virginia Commonwealth University School of Medicine. Suzanne’s interests include research and development, product development, corporate development/alliance management, expertise in life sciences, molecular biology, cannabinoids, oncology, therapeutics, microbiome science, medical foods, nutrition, and skincare.

Articles by Suzanne Kennedy

Plasmid vs. Genomic DNA Extraction: The Difference
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Plasmid vs. Genomic DNA Extraction: The Difference

Most experiments start with a piece of DNA—either plasmid DNA or genomic DNA. And your downstream uses for it dictate how much you need, what contaminants you can tolerate, and your extraction and purification methods. In this article, we explain the key differences between plasmid and genomic DNA extraction methods.

5 Types of Difficult Lab Supervisor and How to Handle Them

5 Types of Difficult Lab Supervisor and How to Handle Them

The lab is a melting-pot full of workers from different cultures and backgrounds, so some conflicts of personality are inevitable. However, when the lab head is the person that you are struggling to get along with, it can make your life a lot harder. Check out some different personality types and get advice on how to work with them effectively.

The Key to Unlocking DNA from FFPE Tissues

The Key to Unlocking DNA from FFPE Tissues

Formalin fixed paraffin embedded (FFPE) tissues are valuable samples that typically come from human specimens collected for examination of the histology of biopsies for the detection of cancer. But each sample contains much more information just waiting to be unlocked. Despite the tiny sample size, DNA can be extracted from the tissue sections and used…

No More White Elephants! – Consider this Before Buying a Real-time PCR Cycler

No More White Elephants! – Consider this Before Buying a Real-time PCR Cycler

Does your lab have a closet full of white elephants; once expensive instruments that are no longer fit for purpose, or have broken down? In many cases, all of that wasted money and resource could have been saved if the buyers had made smart choices about matching the instrument more closely to their needs. A…

There’s No Need To Be Paranoid About RNA Purification
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There’s No Need To Be Paranoid About RNA Purification

RNA purification may be a common procedure in molecular biology but it is by far the one that people fear most. Why? Dreaded RNase. It’s everywhere… all over your bench and pipettes, and floating in the air, waiting for the chance to creep into your prep, shred your RNA into nucleotides, and ruin a day’s…

When Glycogen is not Your Friend – Isolating RNA from Glycogen-Rich Tissues

When Glycogen is not Your Friend – Isolating RNA from Glycogen-Rich Tissues

Bitesize Bio has had a lot to say about RNA isolation, mainly because it is one of the most anxiety-producing requirements for molecular biology; especially when you are first starting out (although isolating proteins from complex samples like soil and stool is far more difficult, let me tell you.  But that’s a future post.)  We’ve…

Troubleshooting RNA Isolation
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Troubleshooting RNA Isolation

As widely used as it is, isolating RNA remains one of the more finicky protocols. Just about anyone who has performed the technique has their own personal tips and tricks to successfully isolate intact RNA from their samples with consistency. Although RNA can be somewhat unpredictable since it is so labile, there are a few…

10 Signs You’ll Like Working in Biotech

10 Signs You’ll Like Working in Biotech

The decision to make the switch from an academic lab and career to a biotech company doesn’t come easy. Many scientists are wary of the lack of independence and doing science for profit.  But there are advantages too. Working in a biotech lab allows you to work on many varied and interesting projects that actually come to…

MIQE Guidelines: Do Your RT-qPCRs Make The Grade?

MIQE Guidelines: Do Your RT-qPCRs Make The Grade?

Northern and Southern blotting are now a thing of the past. They’ve been replaced with a faster and more quantitative technique. No longer do we wait days to know whether a gene is expressed. We can have the answer in 45 minutes! Real-time PCR is now commonly employed in almost all molecular biology laboratories to…

Do Hand Sanitizer and Liquid Hand Soap Remove Viruses?

Do Hand Sanitizer and Liquid Hand Soap Remove Viruses?

While reading my back issues of Applied and Environmental Microbiology (AEM), I came across an interesting paper that detailed an in-depth study on the effectiveness of hand cleaners to remove Norwalk virus (NV) from intentionally contaminated hands. Yes that’s right – intentionally contaminated, and how. The study volunteers allowed a 20% stool suspension containing Norwalk virus to be…

When Your Partner is NOT a scientist

When Your Partner is NOT a scientist

A recent  article published by The Scientist called Power Couples gave advice and examples for scientist couples who have successfully balanced their life at home and in the lab.  It was interesting from the perspective of how two very busy and career motivated people work together to have it all: raise a family, run a lab, and stay in love…

Tech Clinic #5: Copy Number Determination for Plasmid Standard Curves

We received the following question from Bitesize Bio reader, Beheroze Sattha. It relates to a problem with absolute quantification using plasmids for standard curves. Since many people use this technique it is an interesting one question for us to explore, and it also gives us a great opportunity to cover some important tips for performing…

Better Than Betaine: PCR Additives That Actually Work

Better Than Betaine: PCR Additives That Actually Work

If you’re like many researchers, problems with PCR amplifying high GC DNA templates will be a major annoyance for you.  Many strategies developed to overcome this issue. Betaine is the most common PCR additive used to enhance amplification of GC rich sequences because of its ability to dissolve secondary structure that blocks polymerase action.  But…

Read This Before You Design Those qPCR Primers

Read This Before You Design Those qPCR Primers

qPCR is a technique used daily in most labs, but the first step, designing your qPCR primers, can be the biggest obstacle to even getting started. Without a good pair of primers, you can’t start asking the real questions and generating data.  And sometimes the effort involved in optimizing an assay for high efficiency and sensitivity…

Tech Clinic #3: DNA digestion, precipitation and clean-up

Thanks to Bitesize Bio reader, Muthu Arumugam for contacting us about some problems he has been having with restriction digestion and clean up of DNA. I have boiled his query down to four main questions that are pertinent for most molecular biologists, so I hope that Muthu and everyone else can learn something from my…

Tech Clinic #2: Gel Extraction – Avoid/Rescue a Bad 260/230 Ratio

Tech Clinic #2: Gel Extraction – Avoid/Rescue a Bad 260/230 Ratio

Gel extraction — what could be easier? Now we have quick and easy gel extraction kits, we no longer need to use time-consuming old fashioned methods like electro-elution or “freeze and squeeze”. Thank goodness. When Gel Extraction Goes Wrong But even the simplest of procedures can go wrong. Maybe you were distracted, confused, or thought…

Six Important Factors for Successful Reverse Transcription
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Six Important Factors for Successful Reverse Transcription

The reverse transcription (RT) step of RT-PCR for converting RNA to cDNA is critical for accuracy in quantification and for finding low copy messages. Thus, you want to make sure that this step is performed with the highest efficiency but without having to optimize every single step. To help you further in optimizing the RT…