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Anyone who is involved in DNA sequencing in one form or fashion knows there are multiple ways to skin a cat: Sanger-based, next generation (NGS), and of course the new ion torrent sequencing technology. Which technology you use is usually dependent on the questions you’re trying to answer – and how fat your wallet is….
Up till now, we’ve discussed many different ways we can prepare and execute a new career search. In the last series, I discussed getting away from internet job boards and out into the real world. Now, I’m going to challenge you and push most of you way out of your comfort zone. So, there’s XYZ…
Transfection of animal cells has proven an invaluable tool in studies of gene expression, cell behavior, cell processes and molecular genetics. Essentially, transient pores are opened within the cell’s lipid bilayer, allowing insertion of nucleic acids (DNA, RNA, siRNA, RNAi), proteins, nanoparticles, and even antibodies into the cellular milieu. Transfection can be achieved using many…
Whether you’re employing sequencing gels, Sanger-based methods, or the latest in pyrosequencing or ion torrent technologies, obtaining, manipulating and analyzing your sequences has never been easier. Depending on what your goals are, you need to understand the pros and cons of the software. There is a lot of software out there, so do you your…
Fortunately, those of us who have learned how to sequence know that aligning sequences is a lot easier and less time consuming than creating them. Whether you’re employing sequencing gels, Sanger-based methods, or the latest in pyrosequencing or ion torrent technologies, obtaining, manipulating and analyzing your sequences has never been easier. We’re going to take…
Thomas Edison, when asked about his failures in creating the world’s first single carbon filament incandescent lightbulb, said (and I’m paraphrasing here): “I didn’t fail, I simply found 2000 ways how NOT to make a lightbulb – and only one way to make it work”. If only most job-seekers possessed this attitude. It’s amazing the…
Career searches and job hunts are painful: answering ads, resumé tweaking, phone calls, interviews, preparation, sweat, tears, fear of rejection, etc. But it’s funny, even after investing all of this effort, the same mistakes are often made by newbies and veterans alike. In this series of articles, we’ll be talking about a variety of ways…
You’ve masterfully run and transferred your gel, and now it’s time to probe and quantify your protein(s). You’ve got your antibodies and ECL ready to go. Substrate – check. Film cartridge – check. Darkroom – ah yes, that magical place where night vision goggles are required to navigate a veritable minefield of potential chaos. It…
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