Content brought to you by Leica Microsystems
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Array Tomography for SEM 3D Reconstruction
Array tomography (AT) is a 3D image reconstruction technique for high resolution, quantitative analysis of biological structures. For optimal results, ultrathin and ordered sections are an absolute requirement. In this webinar you will get tips and tricks to optimize the workflow of your array tomography: Fast and precise trimming of the sample block-face Adhesion of…
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Expanding the Limits of Electron Microscopy Sample Preparation with the Leica EM ICE High Pressure Freezer
In this webinar you will learn: Improved vitrification: Specimen vitrification without synchronisation fluid Vitrification strategies: Optimized freezing for different techniques Light and electrical stimulation: Dissect cellular processes with millisecond precision A look into the future: freezing of crystals Plunge freezing and cryo imaging of proteins and complexes have revealed new details in understanding the machinery…
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Live Cell Isolation by Laser Microdissection
In this webinar, you will learn: How to best prepare your specimen for live cell isolation by laser microdissection How to optimize your laser microdissection workflow How to avoid common pitfalls of this technique Laser microdissection is a tool for the isolation of homogenous cell populations from their native niches in tissues to downstream molecular…
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LAS X Navigator Tutorial: Navigating live cell imaging
Join Dr. Avi Jacob as he introduces you to the Leica LAS X Navigator, a software module that makes multiparametric acquisition and data review of large data sets easier than you ever thought possible! In this tutorial, Dr. Jacobs will discuss: The advantages of the Navigator How to use the software to take larger fields…
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Super-resolved STED spectroscopy: New insights into molecular membrane dynamics
Molecular interactions are key in cellular signalling. They are often ruled or rendered by the mobility of the involved molecules. We present different tools that are able to determine such mobility and potentially extract interaction dynamics. Specifically, the direct and non-invasive observation of the interactions in the living cell is often impeded by principle limitations…
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Precise and lightning-fast spectral Fluorescence Lifetime Imaging at video rate integrated in a high-end confocal microscope
Förster (Fluorescence) Resonance Energy Transfer (FRET) has become a powerful tool to study protein-protein interactions and signal transduction in living cells. FRET is commonly read out either by detecting the ratio of the donor and acceptor intensities (sensitized emission) or by detecting the excited state lifetime of the donor, which decreases with increasing FRET (Fluorescence…
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How to Conduct Localized Proteomics of Microscopic Regions
In this webinar, you will learn: How to perform isolated proteomics of microscopic regions of interest How to use this technique with FFPE tissue How proteomics can help you understand molecular mechanisms in disease We recently developed a technique that allows localized proteomics of microscopic regions of interest, such as specific cell types or neuropathological features of…
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Enhance Your Imaging with Super-Resolution: Improve Spatial Resolution and Increase Signal to Noise Ratio in Difficult Samples
In these informative case studies, you will learn how: HyVolution 2 can improve the spatial resolution you get from your sample, whilst remaining gentle to the fluorophores HyVolution 2 overcomes issues experienced with other imaging modalities, even in difficult samples such as plant material, increasing resolution and signal to noise ratio, thereby facilitating downstream image…
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Revealing Cellular Dynamics with Millisecond Precision – The New Tool That Turned Electron Micrographs into Motion Pictures of Neural Communication
In this webinar, we will talk about how optogenetics coupled with high-pressure freezing can make this possible. We will discuss how to implement electrical stimulation and why it is superior to light stimulation. We will also discuss the importance of sample processing and the challenges you would face while freezing different types of samples. View…
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Using FIT probes and Super-Resolution Microscopy to Decipher Steps of mRNP Assembly in Developing Oocytes
In this webinar, presented by Dr. Imre Gaspar of the Developmental Biology Unit at EMBL, Heidelberg, you will learn: Importance of mRNA localization and function of mRNPs. Advantages of using fluorogenic FIT probes to visualize mRNPs in vivo and in fixed specimen. How super-resolution microscopy can identify factors required for mRNP biogenesis. Abstract: All mRNA…
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Leica Microsystems Laser Microdissection – Dissection Perfection
Abstract: Leica Microsystems Laser Microdissection systems are the method of choice for laser-based microscopic sample dissection and collection. Laser Microdissection (LMD) is a microscopic technique for isolating homogeneous, specific and pure targets from heterogeneous samples for downstream analysis (DNA, RNA & proteins). In addition, the Leica Microsystems LMD systems can be used with live cell…
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The Best of Both Worlds: Combining Light Sheet and Confocal Microscopy
Abstract: Living cells and organisms often suffer from the high light intensities that are used in conventional imaging. Light sheet microscopy reduces phototoxic effects and bleaching, by only illuminating a specimen in a single plane at a time whilst the signal is detected in a perpendicular direction. In combination with high-speed cameras for image acquisition,…
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Create Publication Quality Fluorescence Microscopy Images with the Help of Leica Microsystems’ Science Lab Resource
Have you ever been shown how to use a microscope properly? Or do you just dive right onto the microscope with little or no training and scant knowledge of the basics, then twiddle knobs, snap photos and expect the publication-quality images to appear? If it’s the latter you are certainly not alone! If only there…
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Microscope maintenance: changing and aligning your mercury burner
Mercury burners are one of the most common light sources used in fluorescence microscopes, producing a wide spectrum of wavelengths making them a great light source for viewing your samples. But they do have their issues – one of which is their propensity to break or become damaged if not used and cared for correctly, which…
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Fundamentals of Fluorescence Microscopy
What you will learn from this webinar: How a fluorescence microscope works What resolution you can expect to achieve from different microscopes How to choose the right fluorophore Four key elements that require optimization for fluoresence microscopy Summary: Fluorescence microscopy has become one of the most important tools in the biologist’s toolbox. In this presentation…
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How to Pick the Right Microscopy Technique
In this webinar you will learn: about the variety of imaging techniques that are available how to pick the right technique to answer your scientific question. Summary: There is a plethora of microscopy techniques available and it can be daunting to figure out which technique is the right one for you. This webinar is designed…
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Basics of Confocal Microscopy
In this webinar you will learn: the basics of confocal laser scanning microscopy why and when you should use confocal microscopy how to get started with confocal microscopy including a demonstration of the key features on a Leica Microsystems confocal microscope Summary: Are you a confocal novice but interested in using this technique for your own research…
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Fluorescence 101: A Beginners Guide to Excitation/Emission, Stokes Shift, Jablonski and More!
You may already use fluorescence as a tool in your microscopy and imaging work, but, do you know exactly what it is? Why are certain proteins and probes fluorescent? What causes this light emitting property? We’ll have a look at these and more questions in this article. Start with a definition We’ll start with a…
