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The more expensive your lab centrifuge, the more sensitive and the easier it is to break. What can we do to give these pricey monsters a long, successful tenure in the lab? Read our 5 easy tips.
If you’re unsure as to how the peer-review process works, the difference between open and double-blind peer review or are wondering if peer review is broken, read on.
Out with the Old… Well-based assays have been the standard for common laboratory experiments, such as fluorescence cytometry. A researcher places a small amount of sample into a well on a plate and assays it, which produces a single data point. However, this so-called single data point is actually an average of the measurements of…
Tiny, furry, spinning around a wheel—few creatures are as endearing as the lab mouse. Trying to obtain reproductive success with them, however, can leave you spinning your own wheels. Why is it that what works so well for the animal facility staff, or experienced technician, seems to be beyond your reach? After all, mice have…
Not every lab has the cash to shell out on fancy equipment. We share some trusty lab hacks and welcome you to share your own with us.
The primers for your gene of interest have finally arrived in the mail, and you’re ready to figure out whether your favorite gene’s expression level is elevated in those precious tissue samples. Only one small last step before you can proceed. Primer validation. This is a standard procedure where you run PCR or qPCR on…
Is ELISA giving you the blues? The frustrating kind, not the lovely kind you get while watching the enzyme substrate reaction! This age old assay has the perks of being quick and fairly simple to perform, but when conditions are not perfect, ELISAs can deliver less than optimal results, and fail to be consistent and…
Some tissues are tricky to work with. This truth was lost on me in the early years of grad school because I worked with liver samples. If you’re extracting RNA from liver samples, you’re likely not losing sleep over your massive RNA yields. But for the folks doing RNA extractions with less willing donors, such…
If you find it very difficult to call your boss by her first name, feel obligated to wear business casual clothes to the lab, or reckon that it’s slightly weird that your fellow PhD candidates are inviting you to a casual night out instead of to the library to study, then like me, you just…
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