Articles by Heinz Reiske:
Synthetic Peptides and Their Uses: Part 1
Are you studying a small peptide or protein? Learn whether using synthetic peptides can save you hours of transfection, protein expression, and purification.

Selection Pressures: Alternatives to Antibiotics in Molecular Cloning
Want to reduce the use of antibiotics in the lab? Start with switching to alternative cloning methods that use alternative selection pressures.

Video Conferencing Services for Scientists
If to you Zoom is just a feature on a camera and BlueJeans is what you wear to work, then let us enlighten you.

A Guide to Gradient Gels: The Why’s and How’s
Fret no more over fuzzy bands! We uncover how gradient gels can give you better results, and maximize your precious samples when performing SDS-PAGE

Going Serum-Free: The How and Why of Removing Serum From Your Media
Cell in culture need serum to survive and grow, right? Or is it? Can your cells survive without serum and why should you try?

Out With the Old: New PubMed Search Tips
PubMed has had a makeover. Find out where your favorite features have moved to and what’s changed in this shiny new version.

Optimizing qPCR Using the Taguchi Method
When optimizing qPCR, you might try trial and error to improve your assay’s performance. Tweak this, tweak that, and the assay works better, right? Sometimes you might get lucky but adjusting single assay components interdependent with others is hardly systematic. You can spend a lot of time and resources hoping that this time, you will…

A Beginner’s Guide to How Blunt-End Cloning Works
Blunt and sticky might sound dull and dirty but knowing how these different cloning methods work is important when choosing which method to use. Here we give you a guide to how blunt-end cloning works. Blunt-end cloning is the cloning of DNA fragments containing no unpaired bases at the 5’ and 3’ prime ends (i.e.…

Getting Sensitive: Diagnostic Sensitivity and Specificity Simplified
What Do We Mean by Diagnostic Sensitivity? In clinical diagnostics, questions about the sensitivity of an assay will inevitably surface. But what does “sensitivity” mean exactly? The lowest quantity of the given analyte that an assay can detect is often called sensitivity – and to be clear, this quantity is the analytical sensitivity or Limit…

The How and Why of Limit of Detection
When developing an assay, whether it is for basic research or for use in diagnostics, you will often be asked about your assay’s sensitivity. This is perhaps one of the most important performance characteristics you can determine for an assay, and in regulated work, such as in vitro diagnostic (IVD) development and clinical diagnostics, it…
