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Heinz Reiske

For the last 17 years, I have held various R&D and management positions at biotech and molecular diagnostics companies. I received my BSc in Biotechnology from Rutgers University and my Ph.D. in Biochemistry from Cornell University. I have always enjoyed teaching others about science and I hope that I can convey some of what I've learned in my career through writing.

Articles by Heinz Reiske:

A Guide to Gradient Gels: The Why’s and How’s

Fret no more over fuzzy bands! We uncover how gradient gels can give you better results, and maximize your precious samples when performing SDS-PAGE

Glasses showing sharper image highlighting how gradient gels produce a sharper band
14 May 2020 Protein Expression and Analysis

Going Serum-Free: The How and Why of Removing Serum From Your Media

Cell in culture need serum to survive and grow, right? Or is it? Can your cells survive without serum and why should you try?

A bottle of serum.
09 Apr 2020 Cells and Model Organisms

Out With the Old: New PubMed Search Tips

PubMed has had a makeover. Find out where your favorite features have moved to and what’s changed in this shiny new version.

31 Mar 2020 Taming the Literature

Optimizing qPCR Using the Taguchi Method

When optimizing qPCR, you might try trial and error to improve your assay’s performance. Tweak this, tweak that, and the assay works better, right? Sometimes you might get lucky but adjusting single assay components interdependent with others is hardly systematic. You can spend a lot of time and resources hoping that this time, you will…

18 Feb 2020 PCR

A Beginner’s Guide to How Blunt-End Cloning Works

Blunt and sticky might sound dull and dirty but knowing how these different cloning methods work is important when choosing which method to use. Here we give you a guide to how blunt-end cloning works. Blunt-end cloning is the cloning of DNA fragments containing no unpaired bases at the 5’ and 3’ prime ends (i.e.…

blunt cut
11 Dec 2019 DNA / RNA Manipulation and Analysis&New England Biolabs

Getting Sensitive: Diagnostic Sensitivity and Specificity Simplified

What Do We Mean by Diagnostic Sensitivity? In clinical diagnostics, questions about the sensitivity of an assay will inevitably surface. But what does “sensitivity” mean exactly? The lowest quantity of the given analyte that an assay can detect is often called sensitivity – and to be clear, this quantity is the analytical sensitivity or Limit…

12 Nov 2019 Chemistry for Biologists&Chemistry for Biologists

The How and Why of Limit of Detection

When developing an assay, whether it is for basic research or for use in diagnostics, you will often be asked about your assay’s sensitivity. This is perhaps one of the most important performance characteristics you can determine for an assay, and in regulated work, such as in vitro diagnostic (IVD) development and clinical diagnostics, it…

Limit of detection assay
05 Sep 2019 Basic Lab Skills and Know-how
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