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Buffers provide a stable chemical environment for all our experiments. But how do buffers work? And how do you pick one? Read on to find out.
In biosciences, we purify our target molecules using chromatography. So, we need a thorough understanding of how chromatography works!
You may be familiar with standard single fragment ligations, but did you know you can ligate multiple fragments into your vector all at the same time! Discover how to perform multiple fragment ligation, including the different methods and troubleshooting tips for when things go wrong.
Want to know the best way to perform relative quantification for your qPCR data? Read on.
Western blot quantification can show how protein expression changes between conditions and treatments. Discover the 4 critical steps you need for quantification.
Got non-specific PCR amplification? You need touchdown PCR! Discover what it is, how it works, and get 5 top tips for performing touchdown PCR.
Pouring and running an agarose gel should be a simple and routine procedure, but there are a surprising number of ways to destroy your agarose gel.
CRISPR isn’t just about DNA editing. Discover how you can use Cas13 proteins in your research to knock down, modify or track RNAs in mammalian cells.
When working with proteins, accurate protein quantification is key for getting reliable data. Here are the top 5 quantification assays.
Are you having problems amplifying GC-rich regions in your PCR reactions? Read this article for 5 easy tips to address these issues!
Can’t be bothered doing a qPCR standard curve? Find out why it’s important to getting great results and how to do it.
Oil immersion microscopy can improve your resolution in microscopy. This article will explain why this is the case and how you can use oil immersion microscopy in the lab!
NMR is a powerful structural tool for studying proteins. Learn how to use multidimensional NMR to identify ligand binding sites and more!
Here we take a closer look at plasmid copy number and examine how it can be manipulated in the lab giving you flexibility in your work.
Cell confluency can affect cell behavior and growth. Find out what confluency is, how to measure it, and why it is a crucial consideration for your experiments.
NMR can generate tons of useful data for protein samples. Learn all about NMR and Protein Dimerization in this easy intro.
Understanding the basic (and simple!) chemistry behind DNA ligation will help you get better DNA ligation results. Learn all about it here.
NMR is a structural tool that plays second fiddle to crystallography. But there’s a cheap, label-free method of investigating protein folding by NMR.
Discover what phosphorylation is, understand why you might want to mimic it, and learn 3 ways to mimic phosphorylation for both in vitro and in vivo applications
You don’t have to be a genius to understand Cryo-EM. Discover the fundamentals of this powerful microscopy tool and what propelled it into the scientific mainstream.
The slow, inching progress of cryo-EM towards the scientific mainstream can be told as a story with three parts. So take a step back and enjoy a short history of cryo-electron microscopy.
You don’t have to be a brainbox to get your samples ready for cryo-EM, but a little wisdom goes a long way. Learn how to tend to your tissues, organize your organelles, and prepare your proteins to get the micrographs you’ve always dreamed of.
Microscopy is a huge and active field. Sometimes, it’s easy to forget the basics. Read our biologists’ guide to electron microscopy techniques.
Microscopy is a huge and active field. Sometimes, it’s easy to forget the basics. Read our biologists’ intro to applications of electron microscopy.
Whether you’re generating, analyzing, or reviewing qPCR data you need to understand how it works and best practices. That’s why we’ve pulled together our top qPCR papers every researcher should read.
Are your plasmid preps giving your poor yields? We’ve got 11 reasons that might be causing it and how to fix them.
Getting to know your protein’s structure can help uncover deeper insights and inspire new hypotheses. Discover how protein data bank files can help.
Why is SDS-PAGE run vertically? Agarose gel electrophoresis is run horizontally, so why not SDS-PAGE? Here are 3 great answers.
Discover ways to perform total RNA extraction from TRIzol to kit-based and even old-school methods.
Circular dichroism is a type of spectroscopy that can tell you the type and percentage of secondary structure units in a protein sample. Here are 5 handy pointers on best circular dichroism practice to set up your experiment properly.
Circular dichroism is a type of spectroscopy that can tell you the type and percentage of secondary structure units in a protein sample. This article will take you through circular dichroism theory and some of its applications.
SDS-PAGE is the technique that unites life scientists. We all perform it during our research to separate protein analytes and, therefore, we all need a good SDS-PAGE gel recipe.
Expressing your protein of interest but not sure if it’s properly folded or struggling with inclusion bodies? Read on to discover advice and tips for battling inclusion bodies and refolding proteins
There are several great protein staining methods, but how do you pick the one that’s appropriate to your intended application? Read on to find out.
Do you want to know about a cool way to detect and tell the difference between virus particles? Then read on to discover flow virometry!
Discover 6 critical scanning electron microscopy sample preparation points you need to know to get the best out of your SEM.
The interaction of proteins with ligands is an important phenomenon. Learn how to detect these interactions (and much more) using the thermal shift assay. In this article, we’ve got you covered with the basic theory and an easy protocol too.
Homology modeling is a powerful tool that enables you to predict protein structures. But how do you do it? Read on to find out.
We’ll show you how to make a DIY stock of chemically competent E. coli, the workhorse in the molecular biology laboratory.
Laemmli buffer is used almost every day by life since researchers. But what do all those components actually do? Find out in this handy article
The eBook with top tips from our Researcher community.