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Search below to delve into the Bitesize Bio archive. Here, you’ll find over two decades of the best articles, live events, podcasts, and resources, created by real experts and passionate mentors, to help you improve as a bioscientist. Whether you’re looking to learn something new or dig deep into a topic, you’ll find trustworthy, human-crafted content that’s ready to inspire and guide you.
Not sure what water of crystallization is? This article explains everything you need to know so that you’re equipped with the knowledge to decide if it matters to your experiments.
Digital images are essential to communicate your data. Get the information and tools you need to take your scientific illustration to the next level!
What is protein crystallography? Whether you are about to enter the world of protein crystallography or work with a protein crystallographer on your grant, this article will be useful.
You know the drill. To prove your theory, you must show the colocalization of X and Y in a cell. Here are 2 ways to reveal protein colocalization.
Discover how Ponceau S works, how to perform staining, and get a detailed guide to interpreting what the staining means when troubleshooting your failed blots.
Whether you want to get started with fluorescence microscopy or already use it, this guide will ensure you know the basics and get the best out of your fluorescence microscopy.
You can build your own PCR machine using tools and equipment you can get easily and without breaking the bank. Well, what are you waiting for?
ECL is an expensive reagent. Why not learn how to make ECL yourself? This cheap and simple option will give you better blots more often!
Learn the basics of how to passage cells in culture with this easy-to-follow guide. While the specifics change per cell line, the basics remain.
Biosafety cabinets keep your work safe from contamination, and you safe from your work. So here are 12 tips for Working in a biosafety cabinet!
Spectral unmixing in flow cytometry is the key to great data from your full spectrum flow cytometry. Get this wrong, and you risk unreliable results. Read our top 7 tips from a flow cytometry core facilities expert to nail your unmixing.
Do you know what each ingredient in cell culture media does? Read on to take a look at the common ingredients and break down their roles.
Not sure what we mean by cell passage number? Confused about how to calculate it? Wondering if there is a maximum number? We explain it all.
We often use size-exclusion chromatography to purify samples. Read this article to learn how to use it to measure sample molecular weight as well!
Need to get a handle on the fundamental differences between HPLC and GC to pick one for your studies? Here’s a quick explainer!
Want to use a cell line but not sure where to start? Or perhaps you’re just curious about the most commonly used cell lines. Our top 5 most commonly used cell lines will help you get a feel for the cells that many researchers turn to.
While using serum in cell culture can keep your cells healthy and happy, there are some notable downsides. We discuss the pros and cons of using serum in cell culture media and how to remove it if you want to eliminate it from your workflow.
Do you need to learn about gas chromatography? This article takes you through the basic principles and instrumentation. With illustrations!
Not sure what FRET is, or just need a refresher on how FRET works? Read our short guide to understand the usefulness of FRET for studying protein-protein interactions.
Designing Cas13 gRNAs is a bit different from the standard Cas9. Read this guide to learn how it differs, and get a step-by-step guide on designing the perfect Cas13 gRNAs.
Discover what RNA quality control is, why it’s so important for your experiments, and how to undertake it with these 3 key considerations.
Buffers provide a stable chemical environment for all our experiments. But how do buffers work? And how do you pick one? Read on to find out.
You may be familiar with standard single fragment ligations, but did you know you can ligate multiple fragments into your vector all at the same time! Discover how to perform multiple fragment ligation, including the different methods and troubleshooting tips for when things go wrong.
Relative Quantification in qPCR compares gene expression levels using either the ΔΔCt or Pfaffl methods. Both rely on housekeeping genes for normalization but differ in handling primer efficiency. The ΔΔCt method assumes equal efficiency, while the Pfaffl method corrects for differences. Selecting stable reference genes or using multiple genes improves accuracy. Understanding these methods helps researchers accurately analyze gene expression changes in their experiments.
Western blot quantification can show how protein expression changes between conditions and treatments. Discover the 4 critical steps you need for quantification.
Got non-specific PCR amplification? You need touchdown PCR! Discover what it is, how it works, and get 5 top tips for performing touchdown PCR.
Pouring and running an agarose gel should be a simple and routine procedure, but there are a surprising number of ways to destroy your agarose gel.
CRISPR isn’t just about DNA editing. Discover how you can use Cas13 proteins in your research to knock down, modify or track RNAs in mammalian cells.
When working with proteins, accurate protein quantification is key for getting reliable data. Here are the top 5 quantification assays.
Are you having problems amplifying GC-rich regions in your PCR reactions? Read this article for 5 easy tips to address these issues!
The qPCR standard curve is essential for validating primer efficiency and ensuring accurate, reproducible qPCR results. It involves amplifying serial dilutions of DNA to assess reaction efficiency, linearity, and repeatability. Proper use of a standard curve helps detect inhibitors, optimize DNA input, and avoid false data. Skipping this step risks unreliable outcomes, making it a critical control in qPCR experiments for bioscience researchers.
Oil immersion microscopy can improve your resolution in microscopy. This article will explain why this is the case and how you can use oil immersion microscopy in the lab!
NMR is a powerful structural tool for studying proteins. Learn how to use multidimensional NMR to identify ligand binding sites and more!
Here we take a closer look at plasmid copy number and examine how it can be manipulated in the lab giving you flexibility in your work.
Cell confluency can affect cell behavior and growth. Find out what confluency is, how to measure it, and why it is a crucial consideration for your experiments.
NMR can generate tons of useful data for protein samples. Learn all about NMR and Protein Dimerization in this easy intro.
Understanding the basic (and simple!) chemistry behind DNA ligation will help you get better DNA ligation results. Learn all about it here.
NMR is a structural tool that plays second fiddle to crystallography. But there’s a cheap, label-free method of investigating protein folding by NMR.
Discover what phosphorylation is, understand why you might want to mimic it, and learn 3 ways to mimic phosphorylation for both in vitro and in vivo applications
You don’t have to be a genius to understand Cryo-EM. Discover the fundamentals of this powerful microscopy tool and what propelled it into the scientific mainstream.
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