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NMR is a structural tool that plays second fiddle to crystallography. But there’s a cheap, label-free method of investigating protein folding by NMR.
Discover what phosphorylation is, understand why you might want to mimic it, and learn 3 ways to mimic phosphorylation for both in vitro and in vivo applications
You don’t have to be a genius to understand Cryo-EM. Discover the fundamentals of this powerful microscopy tool and what propelled it into the scientific mainstream.
The slow, inching progress of cryo-EM towards the scientific mainstream can be told as a story with three parts. So take a step back and enjoy a short history of cryo-electron microscopy.
You don’t have to be a brainbox to get your samples ready for cryo-EM, but a little wisdom goes a long way. Learn how to tend to your tissues, organize your organelles, and prepare your proteins to get the micrographs you’ve always dreamed of.
Microscopy is a huge and active field. Sometimes, it’s easy to forget the basics. Read our biologists’ guide to electron microscopy techniques.
Microscopy is a huge and active field. Sometimes, it’s easy to forget the basics. Read our biologists’ intro to applications of electron microscopy.
Whether you’re generating, analyzing, or reviewing qPCR data you need to understand how it works and best practices. That’s why we’ve pulled together our top qPCR papers every researcher should read.
Are your plasmid preps giving your poor yields? We’ve got 11 reasons that might be causing it and how to fix them.
Getting to know your protein’s structure can help uncover deeper insights and inspire new hypotheses. Discover how protein data bank files can help.
Why is SDS-PAGE run vertically? Agarose gel electrophoresis is run horizontally, so why not SDS-PAGE? Here are 3 great answers.
Discover ways to perform total RNA extraction from TRIzol to kit-based and even old-school methods.
Circular dichroism is a type of spectroscopy that can tell you the type and percentage of secondary structure units in a protein sample. Here are 5 handy pointers on best circular dichroism practice to set up your experiment properly.
Circular dichroism is a type of spectroscopy that can tell you the type and percentage of secondary structure units in a protein sample. This article will take you through circular dichroism theory and some of its applications.
SDS-PAGE is the technique that unites life scientists. We all perform it during our research to separate protein analytes and, therefore, we all need a good SDS-PAGE gel recipe.
Protein crystals are crucial for structure solution via X-ray crystallography but are notoriously hard to grow. We’ve got you covered with 5 clever protein crystallization seeding methods to grow impressive protein crystals.
Expressing your protein of interest but not sure if it’s properly folded or struggling with inclusion bodies? Read on to discover advice and tips for battling inclusion bodies and refolding proteins
There are several great protein staining methods, but how do you pick the one that’s appropriate to your intended application? Read on to find out.
Do you want to know about a cool way to detect and tell the difference between virus particles? Then read on to discover flow virometry!
Discover 6 critical scanning electron microscopy sample preparation points you need to know to get the best out of your SEM.
The interaction of proteins with ligands is an important phenomenon. Learn how to detect these interactions (and much more) using the thermal shift assay. In this article, we’ve got you covered with the basic theory and an easy protocol too.
Protease inhibitors are a requirement in many lab experiments. In this article, we’ll take you through how protease inhibitors work, why we need them, and how to use them correctly and safely.
Homology modeling is a powerful tool that enables you to predict protein structures. But how do you do it? Read on to find out.
We’ll show you how to make a DIY stock of chemically competent E. coli, the workhorse in the molecular biology laboratory.
Laemmli buffer is used almost every day by life since researchers. But what do all those components actually do? Find out in this handy article
How do you make sure that the crystals you have grown are of your target protein? Can you tell salt and protein crystals apart without using X-ray diffraction? Yes, you can. Read on to discover five easy ways to distinguish between salt and protein crystals.
We’re all familiar with DNA and RNA. But have you heard of xeno nucleic acids (XNAs)? Read on to find out how they can be applied in biological research, and how you can start using them in your experiments.
Understanding how DNA extraction kits work is the key to troubleshooting your extraction issues.
Discover how you can visualize that notoriously difficult molecule, RNA using light-up RNA aptamers (LURAs).
This is the first installment in the DNA microarray series where I will introduce the technology and explain the basics.
Whether you’re simply seeking competent E. coli for a transformation experiment or building a panel intended for a whole project, it’s vital that you pick the right strain for your experiment. Find out more in our complete guide to choosing bacterial strains.
Are you struggling with your phenol-chloroform extraction or just looking to maximize the nucleic acid you retrieve? Then read our expert advice to improve your technique.
Phenol extraction is a common method for removing proteins from nucleic acids. Discover how phenol extraction of DNA works.
Ion-exchange chromatography is used to separate and purify proteins based on net charge at a particular pH. Here are the basics about this technique.
Do you need a way to estimate the molecular weight of a sample or protein? We walk you through one simple solution using a gel filtration chromatogram.
Making good quality electrocompetent E. coli is very easy. One morning’s work (with a bit of work ahead of time) is all it takes.
HEK293 cells are one of the most commonly used cell lines. But what are they? Why should you use them? And what does the ‘293’ mean?
Discover how the luciferase reporter assay works and how to get starting using it in your research.
Need to brush up on your alternative splicing knowledge? We’re here to help with our guide to this splicing mechanism.
Want to use Gateway cloning or having trouble using this technology? Find out how it works and get helpful tips to increase your success.
The eBook with top tips from our Researcher community.