Techniques
An Introduction to Circular Dichroism Part 2: Circular Dichroism Practice
Circular dichroism is a type of spectroscopy that can tell you the type and percentage of secondary structure units in a protein sample. Here are 5 handy pointers on best circular dichroism practice to set up your experiment properly.
Read MoreAn Introduction to Circular Dichroism Part 1: Circular Dichroism Theory
Circular dichroism is a type of spectroscopy that can tell you the type and percentage of secondary structure units in a protein sample. This article will take you through circular dichroism theory and some of its applications.
Read MoreA Simple SDS-PAGE Gel Recipe and 10-Step Casting Protocol for Perfect Gels
SDS-PAGE is the technique that unites life scientists. We all perform it during our research to separate protein analytes and, therefore, we all need a good SDS-PAGE gel recipe.
Read More5 Protein Crystallization Seeding Techniques for Bigger and Better Crystals
Protein crystals are crucial for structure solution via X-ray crystallography but are notoriously hard to grow. We’ve got you covered with 5 clever protein crystallization seeding methods to grow impressive protein crystals.
Read MoreComprehensive flow cytometry profiling of the immune system in COVID-19 convalescent individuals
Comprehensive flow cytometry profiling of the immune system in COVID-19 convalescent individuals Available On Demand Speaker Marjorie Pion Principal InvestigatorInstituto de Investigación Sanitaria Gregorio Marañón Marjorie Pion is Principal Investigator and Co-Leader of the Immuno-Regulation Laboratory in the Instituto de Investigación Sanitaria Gregorio Marañón. She obtained her PhD from the University Aix-Marseille, France, in 2002…
Read MoreA Protein Renaturation Toolkit: 21 Tips and Tricks for Refolding Proteins
Expressing your protein of interest but not sure if it’s properly folded or struggling with inclusion bodies? Read on to discover advice and tips for battling inclusion bodies and refolding proteins
Read MoreProtein Staining Methods: An Overview of 3 of the Best
There are several great protein staining methods, but how do you pick the one that’s appropriate to your intended application? Read on to find out.
Read MoreFlow Cytometry Gone Viral: Introducing Flow Virometry
Do you want to know about a cool way to detect and tell the difference between virus particles? Then read on to discover flow virometry!
Read MoreSee the Hidden: Cancer Research
See the Hidden: Cancer ResearchLIVE Online Workshop Imperial College London Edition Available On Demand Leica Microsystems is pleased to welcome you to the 4th virtual edition of our popular See the Hidden Workshop Series hosted by Microscopy Focus, which will explore the next generation of methodologies, techniques, and workflows that are helping to accelerate cancer…
Read MoreHigh-dimensional cytometry data analysis using machine learning
High-dimensional cytometry data analysis using machine learning Available On Demand Speaker Cara E Wogsland, PhD Senior ResearcherKinN Therapeutics AS Dr Cara Wogsland is a Senior Researcher at KinN Therapeutics. She obtained her PhD in the lab of Jonathan Irish at Vanderbilt University Medical Center, which specializes in single-cell techniques, including mass cytometry. Before joining KinN…
Read MoreBoosting signal-to-noise in single cell RNA-seq through molecular depletion
Boosting signal-to-noise in single cell RNA-seq through molecular depletion Available On Demand Speaker Jon Bezney Research Associate III,Research & Development,Jumpcode Genomics Jon Bezney is a Research Associate III at Jumpcode Genomics. Jon received his Bachelor of Science from Yale University in molecular biology. He then spent three years at the Broad Institute of MIT and…
Read MoreScanning Electron Microscopy: 6 SEM Sample Preparation Pointers for Successful Imaging
Discover 6 critical scanning electron microscopy sample preparation points you need to know to get the best out of your SEM.
Read MoreMeltdown: A Hot Introduction to the Thermal Shift Assay
The interaction of proteins with ligands is an important phenomenon. Learn how to detect these interactions (and much more) using the thermal shift assay. In this article, we’ve got you covered with the basic theory and an easy protocol too.
Read MoreProtease Inhibitors 101: How They Work and How to Use Them
Protease inhibitors are a requirement in many lab experiments. In this article, we’ll take you through how protease inhibitors work, why we need them, and how to use them correctly and safely.
Read MoreHomology Modeling in the World of Proteins
Homology modeling is a powerful tool that enables you to predict protein structures. But how do you do it? Read on to find out.
Read MoreHow to Make Your Own Chemically Competent Cells
We’ll show you how to make a DIY stock of chemically competent E. coli, the workhorse in the molecular biology laboratory.
Read MoreMapping microscopic viscosity and temperature using molecular rotors
Mapping microscopic viscosity and temperature using molecular rotorsLIVE WEBINAR Date: Tuesday November 30th 2021Time: LONDON 16:00 | BERLIN 17:00 | DUBAI 20:00 Viscosity is one of the main factors which influence diffusion in condensed media. In a cell, microscopic viscosity (microviscosity) can play a role in several diffusion-mediated processes, such as drug delivery, signaling, and…
Read MoreLaemmli Buffer: What Is It for Anyway?
Laemmli buffer is used almost every day by life since researchers. But what do all those components actually do? Find out in this handy article
Read MoreIsoelectric Focusing: A Simple Way to Enhance Your Protein Separation
Isoelectric focusing is a way of separating proteins and peptides based on their isoelectric point. Read all about this method and its applications right here.
Read More5 Easy Ways to Tell Salt and Protein Crystals Apart
How do you make sure that the crystals you have grown are of your target protein? Can you tell salt and protein crystals apart without using X-ray diffraction? Yes, you can. Read on to discover five easy ways to distinguish between salt and protein crystals.
Read MoreUltramicrotomy for room temperature and cryo applications
Ultramicrotomy for room temperature and cryo applicationsONLINE WORKSHOP Available On Demand Ultramicrotomy is a proven and universally accepted technique for sample preparation for TEM, SEM, AFM, etc. It permits the fine internal structures of samples to be visualized and analyzed at nanometer-scale resolution by producing ultrathin sample sections in a fast and clean manner. A…
Read MoreXeno Nucleic Acids: Essential Tools in Synthetic Biology and Your Research
We’re all familiar with DNA and RNA. But have you heard of xeno nucleic acids (XNAs)? Read on to find out how they can be applied in biological research, and how you can start using them in your experiments.
Read MoreHow DNA Extraction Kits Work in the Lab
Understanding how DNA extraction kits work is the key to troubleshooting your extraction issues.
Read MoreLight-up RNA Aptamers: Illuminating the World of RNA
Discover how you can visualize that notoriously difficult molecule, RNA using light-up RNA aptamers (LURAs).
Read MoreIntroduction to DNA Microarrays
This is the first installment in the DNA microarray series where I will introduce the technology and explain the basics.
Read MoreChoosing the Right Bacterial Strains for Your Lab
Whether you’re simply seeking competent E. coli for a transformation experiment or building a panel intended for a whole project, it’s vital that you pick the right strain for your experiment. Find out more in our complete guide to choosing bacterial strains.
Read MorePractical Application of Phenol-Chloroform Extraction
Are you struggling with your phenol-chloroform extraction or just looking to maximize the nucleic acid you retrieve? Then read our expert advice to improve your technique.
Read MoreThe Basics: How Phenol Extraction of DNA Works
Phenol extraction is a common method for removing proteins from nucleic acids. Discover how phenol extraction of DNA works.
Read MoreTranslational Cancer Research
Translational Cancer ResearchSee the Hidden – LIVE Online Workshop Available On Demand Understanding cancer biology has become increasingly dependent on imaging. High-resolution imaging is vital for studying genetic and cell signaling changes that underlie cancer, whereas live-cell imaging is crucial for a deeper understanding of disease mechanisms. Microscopy techniques are also essential for the study…
Read MoreAll Charged Up: The Basics of Ion-Exchange Chromatography
Ion-exchange chromatography is used to separate and purify proteins based on net charge at a particular pH. Here are the basics about this technique.
Read MoreMeasuring Beyond the Limitations of ELISAs
Measuring Beyond the Limitations of ELISAs Available On Demand Speaker Craig Lanio Field Application ScientistMilliporeSigma Craig is a Field Application Scientist with MilliporeSigma and has been part of the Immunoassay Platform Solutions team for almost 15 years. Prior to coming to MilliporeSigma, he was a scientist with multiple biopharma companies where he participated in every…
Read MoreTissue Processing For Histology: What Exactly Happens?
Tissue processing for histology is a key step between fixation and embedding. We take you through the steps of tissue processing in this simple guide.
Read MoreHyperplex cancer tissue analysis at single cell level with Cell DIVE™
Hyperplex cancer tissue analysis at single cell level with Cell DIVE™ Available On Demand The H2P2 histopathology platform team have been mastering immunohistochemical staining and multiplexing for more than 15 years. With Cell DIVE technology, they offer the scientific community the opportunity to perform hyperplex labeling with up to 50 labels on a single tissue…
Read MoreUsing a Gel Filtration Chromatogram to Estimate Molecular Weight
Do you need a way to estimate the molecular weight of a sample or protein? We walk you through one simple solution using a gel filtration chromatogram.
Read MoreThe Basics of Running a Chromatography Column
Are you preparing to run your first column chromatography experiment? Here are some basics that you should know before you use that precious sample.
Read MoreDIY Electrocompetent E. coli
Making good quality electrocompetent E. coli is very easy. One morning’s work (with a bit of work ahead of time) is all it takes.
Read MoreWhat the HEK? A Beginner’s Guide to HEK293 Cells
If you’re at all familiar with cell culture, you’ve probably heard of HEK293 cells. HEK293 cells are one of the most commonly used cell lines in laboratories across the globe. But what are they? Why should you use them? And what does the ‘293’ mean? Are HEK293T cells the same thing? Read on for answers…
Read MoreFunctional Genomics Screening: From Knockout and Modulation to Single Cell Analysis
Functional Genomics Screening: From Knockout and Modulation to Single Cell Analysis Available On Demand Speaker Gurpreet Balrey, PhD Head of Global Commercial Enablement and EMEA Business HeadMerck KGaA, Darmstadt, Germany Gurpreet attended the University of Nottingham for a Biotechnology degree followed by a PhD in Molecular Biology and Plant Genetic Engineering at the same University.…
Read MoreThe Luciferase Reporter Assay: How it Works
Discover how the luciferase reporter assay works and how to get starting using it in your research.
Read MoreEssential Factors for Successful Gateway Cloning
Want to use Gateway cloning or having trouble using this technology? Find out how it works and get helpful tips to increase your success.
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