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Techniques

A Short History of Cryo-Electron Microscopy

The slow, inching progress of cryo-EM towards the scientific mainstream can be told as a story with three parts. So take a step back and enjoy a short history of cryo-electron microscopy.

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Cryo-EM Sample Prep: 5 Crucial Considerations 

You don’t have to be a brainbox to get your samples ready for cryo-EM, but a little wisdom goes a long way. Learn how to tend to your tissues, organize your organelles, and prepare your proteins to get the micrographs you’ve always dreamed of.

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11 qPCR Papers Every Researcher Should Know

Whether you’re generating, analyzing, or reviewing qPCR data you need to understand how it works and best practices. That’s why we’ve pulled together our top qPCR papers every researcher should read.

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Meltdown: A Hot Introduction to the Thermal Shift Assay

The interaction of proteins with ligands is an important phenomenon. Learn how to detect these interactions (and much more) using the thermal shift assay. In this article, we’ve got you covered with the basic theory and an easy protocol too.

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5 Easy Ways to Tell Salt and Protein Crystals Apart

How do you make sure that the crystals you have grown are of your target protein? Can you tell salt and protein crystals apart without using X-ray diffraction? Yes, you can. Read on to discover five easy ways to distinguish between salt and protein crystals.

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Choosing the Right Bacterial Strains for Your Lab

Whether you’re simply seeking competent E. coli for a transformation experiment or building a panel intended for a whole project, it’s vital that you pick the right strain for your experiment. Find out more in our complete guide to choosing bacterial strains.

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DIY Electrocompetent E. coli

Making good quality electrocompetent E. coli is very easy. One morning’s work (with a bit of work ahead of time) is all it takes.

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