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Discover what hematoxylin and eosin staining is used for and how it works, in this concise guide.
Purified proteins must be kept soluble and active. Discover how to design the perfect protein purification buffer—from additives you can include and their appropriate concentrations to important considerations.
Most experiments start with a piece of DNA—either plasmid DNA or genomic DNA. And your downstream uses for it dictate how much you need, what contaminants you can tolerate, and your extraction and purification methods. In this article, we explain the key differences between plasmid and genomic DNA extraction methods.
Do you prepare samples for electron microscopy and want to save time, money, effort, and frustration? This article provides hands-on advice to help you get the best possible data out of your EM experiments.
Need to add extra nucleotides to your plasmid or other DNA sample? Here’s how to use overhang PCR to easily add extra bases using primers.
Investigating human diseases and genetic variation is complex, but CRISPR-edited induced pluripotent stem cells present a promising alternative to immortalized cell lines. This article delves into genome editing principles and offers practical steps for optimizing research techniques, ensuring more accurate and ethical studies.
We must preserve microorganisms for research continuity and to prevent frustrating delays. Here are several reliable ways to do it.
Learn about four fixatives for histology, which one you should pick, and how. Plus, get some top tips for perfect sample preservation.
So you’ve got your flow cytometry training booked and are one step closer to that precious data. Read our 7 top tips on how to get the most from your flow cytometry training.
Learn how confocal laser scanning microscopy works, its applications, and why it’s great for samples that are too thin to section.
We must lyse cells to extract our samples, but which cell lysis method is best for your cells? Read this article to learn about eight excellent methods.
Dive into the world of protein structure science, where AI is revolutionizing the field by offering accurate predictions and free, open-access tools. In this beginner’s guide, we’ll explore these groundbreaking tools, discuss their strengths and weaknesses, and examine the implications for traditional experimental methods like NMR and X-ray crystallography.
Here’s your reliable and adaptable nuclear extraction protocol. It contains buffer recipes, explanations of what the reagents do, and top tips to boost yields.
Genetic variants are critical to fields like evolution, diagnostics, and medicine—but they’re complex. This article breaks them down.
Maxam–Gilbert Sequencing. Slow and obsolete or niche but powerful? Discover how it works and learn about three modern applications.
Confused about yeast protein extraction methods? Let this article guide you through the process and help you select the most appropriate method based on your research goals and available resources.
Struggling with your cloning? It could be time to try blunt-end cloning. This easy introduction explains what it is, when to use it, and how.
Having problems with your in situ hybridization? We’ve got 7 simple tips to help you get outstanding results.
Not sure what water of crystallization is? This article explains everything you need to know so that you’re equipped with the knowledge to decide if it matters to your experiments.
Digital images are essential to communicate your data. Get the information and tools you need to take your scientific illustration to the next level!
What is protein crystallography? Whether you are about to enter the world of protein crystallography or work with a protein crystallographer on your grant, this article will be useful.
You know the drill. To prove your theory, you must show the colocalization of X and Y in a cell. Here are 2 ways to reveal protein colocalization.
Discover how Ponceau S works, how to perform staining, and get a detailed guide to interpreting what the staining means when troubleshooting your failed blots.
Whether you want to get started with fluorescence microscopy or already use it, this guide will ensure you know the basics and get the best out of your fluorescence microscopy.
You can build your own PCR machine using tools and equipment you can get easily and without breaking the bank. Well, what are you waiting for?
ECL is an expensive reagent. Why not learn how to make ECL yourself? This cheap and simple option will give you better blots more often!
Learn the basics of how to passage cells in culture with this easy-to-follow guide. While the specifics change per cell line, the basics remain.
Biosafety cabinets keep your work safe from contamination, and you safe from your work. So here are 12 tips for Working in a biosafety cabinet!
Spectral unmixing in flow cytometry is the key to great data from your full spectrum flow cytometry. Get this wrong, and you risk unreliable results. Read our top 7 tips from a flow cytometry core facilities expert to nail your unmixing.
Do you know what each ingredient in cell culture media does? Read on to take a look at the common ingredients and break down their roles.
Are you struggling with ugly and unreadable western blots? Here are 3 ways to optimize your western blot transfer and get blots to be proud of.
Not sure what we mean by cell passage number? Confused about how to calculate it? Wondering if there is a maximum number? We explain it all.
We often use size-exclusion chromatography to purify samples. Read this article to learn how to use it to measure sample molecular weight as well!
Need to get a handle on the fundamental differences between HPLC and GC to pick one for your studies? Here’s a quick explainer!
Want to use a cell line but not sure where to start? Or perhaps you’re just curious about the most commonly used cell lines. Our top 5 most commonly used cell lines will help you get a feel for the cells that many researchers turn to.
While using serum in cell culture can keep your cells healthy and happy, there are some notable downsides. We discuss the pros and cons of using serum in cell culture media and how to remove it if you want to eliminate it from your workflow.
Do you need to learn about gas chromatography? This article takes you through the basic principles and instrumentation. With illustrations!
Not sure what FRET is, or just need a refresher on how FRET works? Read our short guide to understand the usefulness of FRET for studying protein-protein interactions.
Designing Cas13 gRNAs is a bit different from the standard Cas9. Read this guide to learn how it differs, and get a step-by-step guide on designing the perfect Cas13 gRNAs.
Discover what RNA quality control is, why it’s so important for your experiments, and how to undertake it with these 3 key considerations.
The eBook with top tips from our Researcher community.