Techniques
How to Measure Molecular Weight Using Size-Exclusion Chromatography (SEC)
We often use size-exclusion chromatography to purify samples. Read this article to learn how to use it to measure sample molecular weight as well!
Read MoreHPLC and GC: 6 Fundamental Differences Made Simple to Enhance Your Research
Need to get a handle on the fundamental differences between HPLC and GC to pick one for your studies? Here’s a quick explainer!
Read MoreTop 5 of the Most Commonly Used Cell Lines
Want to use a cell line but not sure where to start? Or perhaps you’re just curious about the most commonly used cell lines. Our top 5 most commonly used cell lines will help you get a feel for the cells that many researchers turn to.
Read MoreGoing Serum-Free: The How and Why of Removing Serum From Your Media
While using serum in cell culture can keep your cells healthy and happy, there are some notable downsides. We discuss the pros and cons of using serum in cell culture media and how to remove it if you want to eliminate it from your workflow.
Read MoreGas Chromatography: What It Is, How It Works, and 5 Critical Components
Do you need to learn about gas chromatography? This article takes you through the basic principles and instrumentation. With illustrations!
Read MoreHow FRET Works: A Guide to Visualizing Protein Interactions
Not sure what FRET is, or just need a refresher on how FRET works? Read our short guide to understand the usefulness of FRET for studying protein-protein interactions.
Read MoreA Step-by-step Guide to Designing Cas13 gRNAs
Designing Cas13 gRNAs is a bit different from the standard Cas9. Read this guide to learn how it differs, and get a step-by-step guide on designing the perfect Cas13 gRNAs.
Read MoreHow to Perform RNA Quality Control in the Lab: 3 Key Considerations
Discover what RNA quality control is, why it’s so important for your experiments, and how to undertake it with these 3 key considerations.
Read MoreHow Chromatography Works: Separation Science Explained with 5 Simple Examples
In biosciences, we purify our target molecules using chromatography. So, we need a thorough understanding of how chromatography works!
Read MoreMultiple Fragment Ligation: The Why and How
You may be familiar with standard single fragment ligations, but did you know you can ligate multiple fragments into your vector all at the same time! Discover how to perform multiple fragment ligation, including the different methods and troubleshooting tips for when things go wrong.
Read MoreMethods for Relative Quantification of qPCR Data: Yes, There is More Than One
Want to know the best way to perform relative quantification for your qPCR data? Read on.
Read MoreThe 4 Important Steps for Western Blot Quantification
Western blot quantification can show how protein expression changes between conditions and treatments. Discover the 4 critical steps you need for quantification.
Read MoreWhat is Touchdown PCR? A Primer and 5 Easy Tips For Success
Got non-specific PCR amplification? You need touchdown PCR! Discover what it is, how it works, and get 5 top tips for performing touchdown PCR.
Read More5 Ways to Destroy your Agarose Gel and How to Avoid Them!
Pouring and running an agarose gel should be a simple and routine procedure, but there are a surprising number of ways to destroy your agarose gel.
Read MoreCRISPR-Cas13: How to Revolutionize Your RNA Research
CRISPR isn’t just about DNA editing. Discover how you can use Cas13 proteins in your research to knock down, modify or track RNAs in mammalian cells.
Read MoreTop 5 Protein Quantification Assays
When working with proteins, accurate protein quantification is key for getting reliable data. Here are the top 5 quantification assays.
Read MoreThe qPCR Standard Curve: The Key to Good qPCR Data
Can’t be bothered doing a qPCR standard curve? Find out why it’s important to getting great results and how to do it.
Read MoreHow Using Oil Immersion Microscopy Can Increase Your Resolution
Oil immersion microscopy can improve your resolution in microscopy. This article will explain why this is the case and how you can use oil immersion microscopy in the lab!
Read MoreThe Basics of NMR Part 3: Multidimensional NMR
NMR is a powerful structural tool for studying proteins. Learn how to use multidimensional NMR to identify ligand binding sites and more!
Read MoreHow Confluent Are Your Cells? A Beginner’s Guide to Measuring Cell Culture Confluency
Cell confluency can affect cell behavior and growth. Find out what confluency is, how to measure it, and why it is a crucial consideration for your experiments.
Read MoreThe Basics of NMR Part 2: NMR and Protein Dimerization
NMR can generate tons of useful data for protein samples. Learn all about NMR and Protein Dimerization in this easy intro.
Read MoreDNA Ligation: How it Works & 6 Top Tips
Understanding the basic (and simple!) chemistry behind DNA ligation will help you get better DNA ligation results. Learn all about it here.
Read MoreThe Basics of NMR Part 1: Assessing Protein Folding by NMR
NMR is a structural tool that plays second fiddle to crystallography. But there’s a cheap, label-free method of investigating protein folding by NMR.
Read More3 Easy Ways to Mimic Phosphorylation
Discover what phosphorylation is, understand why you might want to mimic it, and learn 3 ways to mimic phosphorylation for both in vitro and in vivo applications
Read MoreWhat Is Cryo-Electron Microscopy? A Brief Introduction
You don’t have to be a genius to understand Cryo-EM. Discover the fundamentals of this powerful microscopy tool and what propelled it into the scientific mainstream.
Read MoreA Short History of Cryo-Electron Microscopy
The slow, inching progress of cryo-EM towards the scientific mainstream can be told as a story with three parts. So take a step back and enjoy a short history of cryo-electron microscopy.
Read MoreHow UV Light Damages DNA and the Havoc it Can Cause to Your Experiments
Learn how UV radiation causes DNA mutations and discover more about the mechanisms underlying skin cancer, as well as the detrimental effects that UV light can have on our DNA-based experiments.
Read MoreCryo-EM Sample Prep: 5 Crucial Considerations
You don’t have to be a brainbox to get your samples ready for cryo-EM, but a little wisdom goes a long way. Learn how to tend to your tissues, organize your organelles, and prepare your proteins to get the micrographs you’ve always dreamed of.
Read MoreThe 2 Main Electron Microscopy Techniques: SEM vs TEM
Microscopy is a huge and active field. Sometimes, it’s easy to forget the basics. Read our biologists’ guide to electron microscopy techniques.
Read MoreApplications of Electron Microscopy: An Easy Intro for Biologists
Microscopy is a huge and active field. Sometimes, it’s easy to forget the basics. Read our biologists’ intro to applications of electron microscopy.
Read More11 qPCR Papers Every Researcher Should Know
Whether you’re generating, analyzing, or reviewing qPCR data you need to understand how it works and best practices. That’s why we’ve pulled together our top qPCR papers every researcher should read.
Read MoreBetter Plasmid Purification: 11 Reasons Your Plasmid Yield is Low
Are your plasmid preps giving your poor yields? We’ve got 11 reasons that might be causing it and how to fix them.
Read MoreProtein Structure Analysis: Extra Information in a Protein Data Bank File
Getting to know your protein’s structure can help uncover deeper insights and inspire new hypotheses. Discover how protein data bank files can help.
Read MoreWhy Is SDS-PAGE Run Vertically? Here are 3 Great Answers
Why is SDS-PAGE run vertically? Agarose gel electrophoresis is run horizontally, so why not SDS-PAGE? Here are 3 great answers.
Read MoreTotal RNA Extraction: Easy Methods for Getting High-Purity RNA
Discover ways to perform total RNA extraction from TRIzol to kit-based and even old-school methods.
Read MoreAn Introduction to Circular Dichroism Part 2: Circular Dichroism Practice
Circular dichroism is a type of spectroscopy that can tell you the type and percentage of secondary structure units in a protein sample. Here are 5 handy pointers on best circular dichroism practice to set up your experiment properly.
Read MoreAn Introduction to Circular Dichroism Part 1: Circular Dichroism Theory
Circular dichroism is a type of spectroscopy that can tell you the type and percentage of secondary structure units in a protein sample. This article will take you through circular dichroism theory and some of its applications.
Read MoreA Simple SDS-PAGE Gel Recipe and 10-Step Casting Protocol for Perfect Gels
SDS-PAGE is the technique that unites life scientists. We all perform it during our research to separate protein analytes and, therefore, we all need a good SDS-PAGE gel recipe.
Read More5 Protein Crystallization Seeding Techniques for Bigger and Better Crystals
Protein crystals are crucial for structure solution via X-ray crystallography but are notoriously hard to grow. We’ve got you covered with 5 clever protein crystallization seeding methods to grow impressive protein crystals.
Read MoreA Protein Renaturation Toolkit: 21 Tips and Tricks for Refolding Proteins
Expressing your protein of interest but not sure if it’s properly folded or struggling with inclusion bodies? Read on to discover advice and tips for battling inclusion bodies and refolding proteins
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