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Microscopy and Imaging

Cryo-electron Tomography for Cell Biology

Cryo-electron Tomography for Cell Biology In this tutorial, you will find: The complete workflow for in situ cryo-electron tomography How subtomogram averaging within the cell yields native-state structures of macromolecular complexes (e.g., the asymmetric and dilated nuclear pore of algae) How mapping these structures back into the native cellular environment reveals new molecular interactions that are only…

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Cryo-Electron Microscopy and the Complexity of Cancer

Cryo-Electron Microscopy and the Complexity of Cancer Shed light on disease with an electron beam, one molecule at a timeIn this tutorial, you will find: How structural biology can change the face of your research The biological challenges of cancer research Advantages of cryo-EM for your research Speaker Simon Poepsel Tutorial abstract Covering both biology…

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Revealing the fastest processes in superresolution – with Lattice SIM and ZEISS Elyra 7

Revealing the fastest processes in superresolution – with Lattice SIM and ZEISS Elyra 7 Speakers Renée M. Dalrymple Tutorial abstract Your life sciences research often requires you to measure, quantify and understand the finest details and sub-cellular structures of your sample. You may be working with tissue, bacteria, organoids, neurons, living or fixed -cells and…

alpha-tubulin labeled with Alexa 647Read More

Laser in a droplet

When you hear about a laser, you likely imagine a medium-size apparatus with a light beam coming out of it, not a bacterium in a drop of liquid. Well, Turkish and British scientists went beyond ordinary imagination – they expressed a fluorescent protein in E.coli and suspended live bacteria in droplets. Illuminated droplets served as…

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Optimal Conditions for Live-Cell Imaging

Live-cell imaging is the investigation of dynamic physiological processes in living cells using time-lapse imaging from milliseconds to hours. Live-cell imaging turns multiple snapshots to movies, which is in contrast to fixed-cell imaging that examines cellular activity at a time point. Typical applications of live-cell imaging that are used to study kinetic events include enzyme…

Rose Spear: Engineering Talent IRead More

Thinking Outside the Box: Microscopy for Immunologists

When you think of an immunologist, you will likely imagine someone who studies the immune system… or maybe a person who speaks in a completely different language (CD? IL? The list goes on.). You may also think of a slew of assays that almost exclusively “belong” to immunologists, including ELISA, ELISpot, Flow Cytometry, chromium release…

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An Introduction to Live Cell Imaging

The term live cell imaging collectively refers to the technologies used to capture images of cells in a living, active state, either as individual static pictures or as time-lapse series. Correspondingly, the applications of live cell imaging can be divided in two broad categories: image recording of cells in their natural, living state observing and…

Microscopic of paramecium and amoebaRead More

Array Tomography for SEM 3D Reconstruction

Array Tomography for SEM 3D Reconstruction Speakers Frank Assen Robert Ranner Tutorial abstract Array tomography (AT) is a 3D image reconstruction technique for high resolution, quantitative analysis of biological structures. For optimal results, ultrathin and ordered sections are an absolute requirement. In this webinar you will get tips and tricks to optimize the workflow of…

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Expanding the Limits of Electron Microscopy Sample Preparation with the Leica EM ICE High Pressure Freezer

Expanding the Limits of Electron Microscopy Sample Preparation with the Leica EM ICE High Pressure Freezer In this tutorial you will learn: Improved vitrification: Specimen vitrification without synchronisation fluid Vitrification strategies: Optimized freezing for different techniques Light and electrical stimulation: Dissect cellular processes with millisecond precision A look into the future: freezing of crystals Speakers…

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Live Cell Isolation by Laser Microdissection

Live Cell Isolation by Laser Microdissection In this tutorial, you will learn: How to best prepare your specimen for live cell isolation by laser microdissection How to optimize your laser microdissection workflow How to avoid common pitfalls of this technique Speakers Dr. Oleg Podgorny Tutorial abstract Laser microdissection is a tool for the isolation of…

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LAS X Navigator Tutorial: Navigating live cell imaging

LAS X Navigator Tutorial: Navigating live cell imaging Speakers Dr. Avi Jacob Tutorial abstract Join Dr. Avi Jacob as he introduces you to the Leica LAS X Navigator, a software module that makes multiparametric acquisition and data review of large data sets easier than you ever thought possible! In this tutorial, Dr. Jacobs will discuss:…

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Reliable quantification of fluorescence images: from raw data to reproducible results

Reliable quantification of fluorescence images: from raw data to reproducible results In this tutorial, you will find: How to overcome issues with stage drift and crosstalk between channels How to obtain reliable images and reproducible quantification results How to proceed with advanced image analysis after restoring images Speakers Vincent SchoonderwoerSenior Imaging/Application Specialist, SVI Tutorial abstract…

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Super-resolved STED spectroscopy: New insights into molecular membrane dynamics

Super-resolved STED spectroscopy: New insights into molecular membrane dynamics Speakers Christian Eggeling Tutorial abstract Molecular interactions are key in cellular signalling. They are often ruled or rendered by the mobility of the involved molecules. We present different tools that are able to determine such mobility and potentially extract interaction dynamics. Specifically, the direct and non-invasive…

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An Introduction to Cardiac Optical Mapping

What Is Optical Mapping and How Is It Used? Synchronisation of the contraction of heart muscle is essential for the efficient pumping of blood through the circulatory system. Cardiac contraction is controlled by the regulated spread of electrical impulses from cell-to-cell within the heart.  In pathological conditions, these electrical impulses can become disordered and lead…

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New-ISH on the Block: Introduction to RNAscope®

When sensitive detection of RNA is required, many scientists turn to qPCR as it is a versatile technique that can detect many different types of RNAs from mRNA, non-coding RNA, to microRNA. However, if you also require spatial information, like which cells are producing your RNA, the technology of choice has historically been in situ…

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Precise and lightning-fast spectral Fluorescence Lifetime Imaging at video rate integrated in a high-end confocal microscope

Precise and lightning-fast spectral Fluorescence Lifetime Imaging at video rate integrated in a high-end confocal microscope Speakers Kees JalinkProfessor, The Netherlands Cancer Institute Tutorial abstract Förster (Fluorescence) Resonance Energy Transfer (FRET) has become a powerful tool to study protein-protein interactions and signal transduction in living cells. FRET is commonly read out either by detecting the…

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Protein Colocalization: You Know They Overlap, but Can You Prove It?

Microscopy by default is a technique that allows us to observe, rather than measure, biological events and make conclusions based on what we see, rather than based on some calculations. The topic of this article may therefore seem a little surprising since it deals with the measurements of colocalization. While there are situations where you…

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Your guide to Complete and Fast 3D Image Analysis in Microscopy

Your guide to Complete and Fast 3D Image Analysis in Microscopy Join Dr. Chris Zugates as he takes you through a typical workflow of image analysis, learn how to easily process your data and find the objects of interest quickly and interactively creating meaningful results for publication.In this tutorial, you will find: How to handle…

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Automated Image Analysis – the Future of Data Acquisition?

Automated image analysis uses finely tuned software to extract data from digital images. Algorithms recognize specific shapes and patterns in the images and gather quantitative information that is then used for further data analysis. The pharmaceutical and biological research industries have benefitted greatly from this technology, which allows researchers to analyze hundreds—if not thousands—of samples…

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Safranin: Cheap Stain to Visualize Chromosomes

As an undergraduate student, one of the first experiments I did was staining chromosomes in mitotically active onion root tip cells. The stains that are conventionally used for this purpose are acetocarmine or aceto-orcein (which smell like vinegar). However, the cost of these stains is quite high. Personally, I find safranin, which is another stain, more…

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