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Microscopy and Imaging

Revealing functional mechanisms of synaptic transmission by high-pressure freezing

Revealing functional mechanisms of synaptic transmission by high-pressure freezing On Demand Speakers Frédéric Leroux Advanced workflow specialist, Leica Microsystems Frédéric Leroux completed his Master degree in Biology in 2007 at the University of Ghent where he gained experience in biological EM sample preparation. In 2008, he moved to the physics department at the University of…

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Making the Perfect Blood Smear

Stop wasting time throwing out slide after slide trying to create a readable blood smear. Read our how-to guide for creating the perfect blood smear slide.

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What Can Electron Microscopy Do for You?

The electron microscope (EM) – where electrons, rather than photons, make the image – fell out of fashion for a while, but it has come back refreshed. Modern electron microscopes cost less, use less electricity, and are generally easier to maintain than the older models, so it is likely that you can get your hands on one. Read on to learn more about this technique, and how to implement it into your research.

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Workflows and instrumentation for cryo-electron microscopy: an overview

Workflows and instrumentation for cryo-electron microscopy: an overview Now On Demand Speaker Frédéric Leroux Advanced workflow specialist, Leica Microsystems Frédéric Leroux completed his Master degree in Biology in 2007 at the University of Ghent where he gained experience in biological EM sample preparation. In 2008, he moved to the physics department at the University of…

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Microscope Disinfection: A Quick Guide

Shared microscopes have the potential to get dirty and spread nasties between samples and users. Check out our quick guide on how to clean and disinfect your microscope.

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Making a Mark: How to Set up Your Wound Healing Assay 

Are you wanting to study cell migration and need a way to quantify it? A wound healing assay is one of the earliest and simplest in-vitro assays developed to study cell migration. Collective cell migration is a characteristic behaviour seen in wound repair, cancer invasion, cancer metastasis, immune response, angiogenesis and embryonic morphogenesis. Depending on…

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How to Totally Nail Your First in situ Hybridization

It’s Monday morning and you’re about to start your first in situ hybridization (ISH). You glanced at the protocol on Friday and it seemed pretty easy. You just do a lot of washes and incubations, right? WRONG. ISH can be a nightmare. If you’re not well prepared, you’ll most likely be tearing your hair out…

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Optimized cryo workflows using the vacuum cryo transfer system VCT500

Optimized cryo workflows using the vacuum cryo transfer system VCT500 In this tutorial, you will find: How to use the vacuum cryo transfer system The advantages of the EM VCT500 and EM VCM for different cryo workflows About their flexible integration with different analysis systems Speakers Dr. Julia KönigProduct Manager EM sample preparation for cryo-workflows…

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THUNDER Imagers: High performance, versatility and ease-of-use for your everyday imaging workflows.

THUNDER Imagers: High performance, versatility and ease-of-use for your everyday imaging workflows. In this webinar, you will learn: How to get sharp, high quality images from thick 3-dimensional microscope samples How to shorten your imaging workflow from image acquisition to data analysis A broad spectrum of imaging workflows for a range of life science applications…

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Understanding Malaria Parasite Pathogenesis by Cryo-Electron Microscopy

Understanding Malaria Parasite Pathogenesis by Cryo-Electron Microscopy In this tutorial, you will learn: The mechanisms by which the plasmodium translocon of exported proteins (PTEX)  enables the  Plasmodium parasite to take over red blood cells How single-particle cryo-electron microscopy enables us to gain insight into the PTEX molecular structure The opportunity this presents for structure-based design…

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An Introduction to Digital Images in Publications

Previously, I wrote about the different ways to use scientific illustration to communicate research. In this article, I will introduce the technical aspects of digital images and the most common image file types that scientists will encounter when preparing images for publication. Raster Versus Vector Digital images are classified based on how information is stored.…

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Basics of Fluorescence Lifetime Imaging and FLIM Applications to FRET Biosensors

Basics of Fluorescence Lifetime Imaging and FLIM Applications to FRET Biosensors In this tutorial you will learn: How researchers (Anzilotti 2019) used FLIM to elucidate the mechanisms responsible for a B cell deficiency A new approach to measure the cellular function of the Zn2+ transporter ZIP7 in primary mouse cells, comparing wild-type and CRISPR-Cas9 derived mutant cells…

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How to Weigh Molecules with Light Using Mass Photometry

How to Weigh Molecules with Light Using Mass Photometry In this tutorial, you will learn:    How mass photometry offers a universal way to study biomolecules    The strengths and limitations of mass photometry    How to use mass photometry to: accurately determine the degree of heterogeneity of a sample quantify the strength and kinetics of molecular interactions…

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The New ZEISS LSM 9 Family: Adding the Multiplex Factor to Your Confocal Imaging

The New ZEISS LSM 9 Family: Adding the Multiplex Factor to Your Confocal Imaging In this tutorial on confocal imaging, you will learn how you can: Capture weaker signals—and still get sound, reproducible data. Reach faster volumetric imaging without sacrificing resolution Increase data throughput for your imaging needs Speaker Joseph HuffSolution Manager/Application Development Engineer at…

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Cryofixation and Chemical Fixation for Electron Microscopy

Specimen preparation is the most important aspect of biological electron microscopy (EM), as it influences everything from the preservation of the sample itself to the kind of information that can be obtained. It is vital to define the questions you are asking of the sample before embarking on an electron microscopy project. This enables you…

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Ultramicrotomy for Electron Microscopy

Ultramicrotomy is the process by which a sample is cut into very thin slices or “sections”, usually for imaging by transmission electron microscopy (TEM) or relatively new techniques using scanning electron microscopy (See Array tomography in three dimensional scanning electron microscopy for biology). This technique requires a bit of finesse, and this article will help…

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Microscope Finder Slides: Their History, Development, and Use

Have you ever been looking through a box of slides and found something that you want to image or look at later, or even show to one of your colleagues or supervisor? Finding that exact spot on the slide at a later date can prove to be difficult- using a marker pen on the coverslip…

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Cryo-electron Tomography for Cell Biology

Cryo-electron Tomography for Cell Biology In this tutorial, you will find: The complete workflow for in situ cryo-electron tomography How subtomogram averaging within the cell yields native-state structures of macromolecular complexes (e.g., the asymmetric and dilated nuclear pore of algae) How mapping these structures back into the native cellular environment reveals new molecular interactions that are only…

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Cryo-Electron Microscopy and the Complexity of Cancer

Cryo-Electron Microscopy and the Complexity of Cancer Shed light on disease with an electron beam, one molecule at a timeIn this tutorial, you will find: How structural biology can change the face of your research The biological challenges of cancer research Advantages of cryo-EM for your research Speaker Simon Poepsel Tutorial abstract Covering both biology…

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Revealing the fastest processes in superresolution – with Lattice SIM and ZEISS Elyra 7

Revealing the fastest processes in superresolution – with Lattice SIM and ZEISS Elyra 7 Speakers Renée M. Dalrymple Tutorial abstract Your life sciences research often requires you to measure, quantify and understand the finest details and sub-cellular structures of your sample. You may be working with tissue, bacteria, organoids, neurons, living or fixed -cells and…

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Laser in a droplet

When you hear about a laser, you likely imagine a medium-size apparatus with a light beam coming out of it, not a bacterium in a drop of liquid. Well, Turkish and British scientists went beyond ordinary imagination – they expressed a fluorescent protein in E.coli and suspended live bacteria in droplets. Illuminated droplets served as…

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Thinking Outside the Box: Microscopy for Immunologists

When you think of an immunologist, you will likely imagine someone who studies the immune system… or maybe a person who speaks in a completely different language (CD? IL? The list goes on.). You may also think of a slew of assays that almost exclusively “belong” to immunologists, including ELISA, ELISpot, Flow Cytometry, chromium release…

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Array Tomography for SEM 3D Reconstruction

Array Tomography for SEM 3D Reconstruction Speakers Frank Assen Robert Ranner Tutorial abstract Array tomography (AT) is a 3D image reconstruction technique for high resolution, quantitative analysis of biological structures. For optimal results, ultrathin and ordered sections are an absolute requirement. In this webinar you will get tips and tricks to optimize the workflow of…

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Expanding the Limits of Electron Microscopy Sample Preparation with the Leica EM ICE High Pressure Freezer

Expanding the Limits of Electron Microscopy Sample Preparation with the Leica EM ICE High Pressure Freezer In this tutorial you will learn: Improved vitrification: Specimen vitrification without synchronisation fluid Vitrification strategies: Optimized freezing for different techniques Light and electrical stimulation: Dissect cellular processes with millisecond precision A look into the future: freezing of crystals Speakers…

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Live Cell Isolation by Laser Microdissection

Live Cell Isolation by Laser Microdissection In this tutorial, you will learn: How to best prepare your specimen for live cell isolation by laser microdissection How to optimize your laser microdissection workflow How to avoid common pitfalls of this technique Speakers Dr. Oleg Podgorny Tutorial abstract Laser microdissection is a tool for the isolation of…

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LAS X Navigator Tutorial: Navigating live cell imaging

LAS X Navigator Tutorial: Navigating live cell imaging Speakers Dr. Avi Jacob Tutorial abstract Join Dr. Avi Jacob as he introduces you to the Leica LAS X Navigator, a software module that makes multiparametric acquisition and data review of large data sets easier than you ever thought possible! In this tutorial, Dr. Jacobs will discuss:…

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Reliable quantification of fluorescence images: from raw data to reproducible results

Reliable quantification of fluorescence images: from raw data to reproducible results In this tutorial, you will find: How to overcome issues with stage drift and crosstalk between channels How to obtain reliable images and reproducible quantification results How to proceed with advanced image analysis after restoring images Speakers Vincent SchoonderwoerSenior Imaging/Application Specialist, SVI Tutorial abstract…

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Super-resolved STED spectroscopy: New insights into molecular membrane dynamics

Super-resolved STED spectroscopy: New insights into molecular membrane dynamics Speakers Christian Eggeling Tutorial abstract Molecular interactions are key in cellular signalling. They are often ruled or rendered by the mobility of the involved molecules. We present different tools that are able to determine such mobility and potentially extract interaction dynamics. Specifically, the direct and non-invasive…

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An Introduction to Cardiac Optical Mapping

What Is Optical Mapping and How Is It Used? Synchronisation of the contraction of heart muscle is essential for the efficient pumping of blood through the circulatory system. Cardiac contraction is controlled by the regulated spread of electrical impulses from cell-to-cell within the heart.  In pathological conditions, these electrical impulses can become disordered and lead…

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Precise and lightning-fast spectral Fluorescence Lifetime Imaging at video rate integrated in a high-end confocal microscope

Precise and lightning-fast spectral Fluorescence Lifetime Imaging at video rate integrated in a high-end confocal microscope Speakers Kees JalinkProfessor, The Netherlands Cancer Institute Tutorial abstract Förster (Fluorescence) Resonance Energy Transfer (FRET) has become a powerful tool to study protein-protein interactions and signal transduction in living cells. FRET is commonly read out either by detecting the…

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Protein Colocalization: You Know They Overlap, but Can You Prove It?

Microscopy by default is a technique that allows us to observe, rather than measure, biological events and make conclusions based on what we see, rather than based on some calculations. The topic of this article may therefore seem a little surprising since it deals with the measurements of colocalization. While there are situations where you…

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Your guide to Complete and Fast 3D Image Analysis in Microscopy

Your guide to Complete and Fast 3D Image Analysis in Microscopy Join Dr. Chris Zugates as he takes you through a typical workflow of image analysis, learn how to easily process your data and find the objects of interest quickly and interactively creating meaningful results for publication.In this tutorial, you will find: How to handle…

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Automated Image Analysis – the Future of Data Acquisition?

Automated image analysis uses finely tuned software to extract data from digital images. Algorithms recognize specific shapes and patterns in the images and gather quantitative information that is then used for further data analysis. The pharmaceutical and biological research industries have benefitted greatly from this technology, which allows researchers to analyze hundreds—if not thousands—of samples…

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Safranin: Cheap Stain to Visualize Chromosomes

As an undergraduate student, one of the first experiments I did was staining chromosomes in mitotically active onion root tip cells. The stains that are conventionally used for this purpose are acetocarmine or aceto-orcein (which smell like vinegar). However, the cost of these stains is quite high. Personally, I find safranin, which is another stain, more…

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Get the Best Out of Your Microscopy Images with Huygens Batch Express

Get the Best Out of Your Microscopy Images with Huygens Batch Express In this tutorial, you will learn about the advantages of deconvolving all your images fully automatically.In this tutorial, you will find: How to improve the quality of your images with the new Batch Express How to increase resolution and signal of all your…

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Get Your Microscopy Mojo Back with Our Image Acquisition and Processing Tips

Ever since the invention of the first compound microscope by Zacharias Jansen in 1590, our understanding of the microscopic world has grown exponentially. Microscopes have evolved from mere assemblies of magnifying lenses to extremely powerful tools for visualization on the atomic scale. You can find a wealth of information on the workings of a microscope…

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