Microscopy and Imaging
Beginners Guide to PALM Sample Preparation
Good PALM sample preparation is the key to great images. Find out how to choose the right fluorescent proteins and learn some tips and tricks for sample prep.
Read MoreTurn a Smartphone into a Digital Microscope for Only 3 Cents
Want to build your own microscope for almost nothing? You probably already have most of the tools that you need right in your lab or at home! Here’s how.
Read MoreTrapping Objects With Light: From Star Wars to Neurons
Can lasers be used to trap and move objects? Sure they can! Read on to know how you can use lasers in a cool technique called optical tweezers to manipulate minuscule objects under the microscope.
Read MoreHow Histology Slides are Prepared
Ever wondered what magic happens to turn your samples into histology slides? Find out the 5 simple steps for histology slide preparation.
Read More7 Steps to Avoid Photodamage in Fluorescent Live-cell Imaging
Don’t get overexcited but we’ve got 7 top tips to help you minimize photodamage during your fluorescent live-cell imaging experiments.
Read MoreHow to Measure Myelin Thickness Manually (and Other Tedious Lab Tasks) and Keep Your Sanity
Tips on staying sane from a student survivor of tedious lab tasks.
Read MoreStructured Illumination Microscopy (SIM) – An Introduction
Discover how structured illumination microscopy works can help you see things in greater detail.
Read MoreMaking the Perfect Blood Smear
Stop wasting time throwing out slide after slide trying to create a readable blood smear. Read our how-to guide for creating the perfect blood smear slide.
Read MoreWhat Can Electron Microscopy Do for You?
The electron microscope (EM) – where electrons, rather than photons, make the image – fell out of fashion for a while, but it has come back refreshed. Modern electron microscopes cost less, use less electricity, and are generally easier to maintain than the older models, so it is likely that you can get your hands on one. Read on to learn more about this technique, and how to implement it into your research.
Read MoreMicroscope Disinfection: A Quick Guide
Shared microscopes have the potential to get dirty and spread nasties between samples and users. Check out our quick guide on how to clean and disinfect your microscope.
Read MoreImaging and Analyzing Your Wound Healing Assay
Don’t be daunted by imaging and analyzing your wound healing assay; our top tips will get it looking perfect.
Read MoreMaking a Mark: How to Set up Your Wound Healing Assay
Wound healing assay is one of the earliest and simplest in-vitro assays for studying cell migration. Discover if this assay is right for you and learn how to set it up to get reproducible data.
Read MoreCryofixation and Chemical Fixation for Electron Microscopy
Sample fixation for electron microscopy can be done using either chemical fixation or cryofixation. Discover the benefits and drawbacks of each of these methods.
Read MoreUltramicrotomy for Electron Microscopy
Ultramicrotomy is the process by which a sample is cut into very thin slices or “sections”, usually for imaging by transmission electron microscopy (TEM) or relatively new techniques using scanning electron microscopy (See Array tomography in three dimensional scanning electron microscopy for biology). This technique requires a bit of finesse, and this article will help…
Read MoreMicroscope Finder Slides: Their History, Development, and Use
Have you ever been looking through a box of slides and found something that you want to image or look at later, or even show to one of your colleagues or supervisor? Finding that exact spot on the slide at a later date can prove to be difficult- using a marker pen on the coverslip…
Read MoreLaser in a droplet
When you hear about a laser, you likely imagine a medium-size apparatus with a light beam coming out of it, not a bacterium in a drop of liquid. Well, Turkish and British scientists went beyond ordinary imagination – they expressed a fluorescent protein in E.coli and suspended live bacteria in droplets. Illuminated droplets served as…
Read MoreThinking Outside the Box: Microscopy for Immunologists
We explore microscopy for immunologists and bring them into the light and away from the 96-well plate, at least for some of the time.
Read MoreAn Introduction to Cardiac Optical Mapping
What Is Optical Mapping and How Is It Used? Synchronisation of the contraction of heart muscle is essential for the efficient pumping of blood through the circulatory system. Cardiac contraction is controlled by the regulated spread of electrical impulses from cell-to-cell within the heart. In pathological conditions, these electrical impulses can become disordered and lead…
Read MoreNew-ISH on the Block: Introduction to RNAscope®
RNAscope is a new method of quantitative RNA in situ-hybridization that has taken laboratories by storm. Learn advantages over traditional techniques and how RNAscope works in this introduction article.
Read MoreAutomated Image Analysis – the Future of Data Acquisition?
Automated image analysis uses finely tuned software to extract data from digital images. Algorithms recognize specific shapes and patterns in the images and gather quantitative information that is then used for further data analysis. The pharmaceutical and biological research industries have benefitted greatly from this technology, which allows researchers to analyze hundreds—if not thousands—of samples…
Read MoreSafranin: Cheap Stain to Visualize Chromosomes
As an undergraduate student, one of the first experiments I did was staining chromosomes in mitotically active onion root tip cells. The stains that are conventionally used for this purpose are acetocarmine or aceto-orcein (which smell like vinegar). However, the cost of these stains is quite high. Personally, I find safranin, which is another stain, more…
Read MoreGet Your Microscopy Mojo Back with Our Image Acquisition and Processing Tips
Ever since the invention of the first compound microscope by Zacharias Jansen in 1590, our understanding of the microscopic world has grown exponentially. Microscopes have evolved from mere assemblies of magnifying lenses to extremely powerful tools for visualization on the atomic scale. You can find a wealth of information on the workings of a microscope…
Read MoreColor Transmission Electron Microscopy
There are two types of electron microscopy—transmission electron microscopy (TEM) and scanning electron microscopy (SEM). SEM creates fascinating 2D images by bouncing electrons off the surface of the sample. I highly recommend searching for SEM samples on Google images. There are a wide variety of applications for electron microscopy. While SEM images are aesthetically amazing,…
Read MoreFrom Cells to Scope: Chamber Slide Immunochemistry
Immunolabeling is the tried-and-true immunochemistry method of getting the stain you want onto the molecular target you want. Whether that target is contained within a large region of tissue (immunohistochemistry) or inside a single cell (immunocytochemistry), the ability to accurately label large numbers of samples will simplify your workflow and help you to achieve excellent…
Read MoreLasers for Confocal Microscopy: Part II
Continuing from our first article on lasers for confocal microscopy, we will now discuss two specialized types of lasers: lasers for two-photon excitation and tunable, white light lasers. We will also discuss the applications of the two lasers. Lasers for Two-Photon Excitation The two-photon absorption phenomenon was first described for microscopy in 1931. Here, the…
Read MoreThings to Consider When Buying a Microscope Camera (Part 2)
In the first part of this series, we discussed the differences between a color and a monochrome microscope camera and when one is advantageous over the other. We also touched on the subject of optimal camera resolution for a given imaging system. In this part, we will tackle a few additional camera specifications and how…
Read MoreThe A to Z of Histological Stains
From Alkaline phosphatase to Warthin-Starry, we take you through the various histology stains available.
Read MoreThings to Consider When Buying a Microscope Camera (Part 1)
Purchasing a microscope camera is one of the most daunting tasks you might have to undertake. Before you set out to buy that camera, carefully consider your applications. Things like sample brightness or the speed of the phenomenon you are trying to capture can dictate your choices. Also, this is the time to make peace…
Read MoreAll You Need to Know (and More) About Embedding for Electron Microscopy
Interested in the detailed structure of your tissue? High-resolution imaging techniques, such as brain electron microscopy, provide an intricate view of your tissue. While it may be a rather complicated procedure with nasty chemicals, the advantages of epon embedding can make it the best choice for morphological studies. The hard blocks are excellent for structural…
Read MoreTissue Embedding Throwdown: Paraffin vs OCT vs Resin
Tissue embedding and sectioning is a backbone of many biological research labs. While commiserating with other grad students over tedious hours spent in the lab, you’re probably aware that there is more than one way to slice up a chunk of tissue. We’ve previously introduced what to consider when choosing a tissue embedding medium and…
Read MoreHow to Use CellProfiler for Cell Imaging
Are you trying to figure out how to calculate intensities of fluorescently-labeled single cells? Do you have cells at high densities or present in clusters? Are you worried that your current cell imaging analysis software is unable to mark clear boundaries around each cell in a cell cluster? Don’t fear, because CellProfiler 2.1 is here to…
Read MoreHow to Obtain Stellar Staining with Fluorescent IHC
If you want the kind of fluorescent IHC images worth those extra color publication charges, you’ve come to the right place. Read on for tips and tricks to getting stellar IHC staining.
Read MoreUnpacking the Daunting Task of Stereology for Electron Microscopy
Unpacking the Daunting Task of Stereology for Electron Microscopy Electron microscopy provides fantastic detail and resolution, with brain electron microscopy allowing visualisation of neurons and their individual synaptic connections. You may find yourself needing to count these neurons or connections, which can easily go into the billions. But counting these one-by-one isn’t really feasible. This…
Read MoreDetecting Cell Apoptosis on Tissue Slides
Apoptosis, or programmed cell death, can be detected on tissue slides using stains in conjunction with immunohistochemistry and/or reporter assays.
Read MoreAutomated Microscopy
The traditional microscope that you know and love is operated manually. Picture the scene: the microscopist chooses the light source, gently places the sample the moveable stage, selects the objective lens, and scans to select the field of view. This process is perfect for processing and analyzing a small number of samples per day. But…
Read MoreIn Situ Zymography: Let’s Catch that Enzyme in Action!
In situ zymography (ISZ) is the best choice to study proteases. Proteases are a challenge to study as proteases are extremely potent enzymes. As such, they need to be controlled at multiple levels to prevent them from being unleashed and making a cellular mess. Regulation of their activity occurs at virtually all levels: transcriptional control,…
Read MoreAutomated Cell Counting with a Fluorescent Twist
Cell counting is the bane of existence of many researchers. Countless hours spent in front of the microscope with a haemocytometer on the stand and a manual tally (or “clicker”) in hand can be really daunting. Not to mention that no one will ever double check your count if you don’t take a picture. Those…
Read MoreAnalyze Immunostained Slides with Semiquantitative Scoring
A routine task in the lab is to investigate the presence of your favorite protein in a range of histological samples. No doubt, staining your tissue sections using good old immunohistochemistry (IHC) would be your first choice. You just got to love a technique that has celebrated its 70th birthday, and is still used in…
Read MoreTips for Peering into the Interior of Mice Using Intravital Microscopy
Techniques to study entire tissues, such as brain imaging microscopy, provide great insight into the biology of the whole tissue, rather than just individual cells. Taking this one step further is intravital microscopy (IVM); a newer approach for the imaging of living tissues and organs in live animals. A wide variety of organs can be…
Read MoreData Analysis for Three-dimensional Volume Scanning Electron Microscopy
In recent years, three-dimensional (3D) scanning electron microscopy techniques have gained recognition in the biological sciences. In particular, array tomography, serial block face scanning electron microscopy (SBFSEM) and focused ion beam scanning electron microscopy (FIBSEM) (described in Three-Dimensional Scanning Electron Microscopy for Biology) have shown an increase in biological applications, elucidating ultrastructural details of cells…
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