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Microscopy and Imaging

From Organs to Tissues to Cells: Analyzing 3D Specimens with Widefield Microscopy

From Organs to Tissues to Cells: Analyzing 3D Specimens with Widefield Microscopy On Demand Speakers Dr. Falco Krüger Advanced Workflow Specialist Widefield Life Science Research DivisionLeica Microsystems Falco did his PhD in Biology at the Plant Cell Biology chair of Prof. Karin Schumacher at the Centre for Organismal Studies (COS) in Heidelberg, Germany. For his…

HeLa cell spheroid - THUNDER Imager 3D Live Cell & 3D Cell CultureRead More

Revealing functional mechanisms of synaptic transmission by high-pressure freezing

Revealing functional mechanisms of synaptic transmission by high-pressure freezing On Demand Speakers Frédéric Leroux Advanced workflow specialist, Leica Microsystems Frédéric Leroux completed his Master degree in Biology in 2007 at the University of Ghent where he gained experience in biological EM sample preparation. In 2008, he moved to the physics department at the University of…

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Making the Perfect Blood Smear

Stop wasting time throwing out slide after slide trying to create a readable blood smear. Read our how-to guide for creating the perfect blood smear slide.

Scientist holding a blood smear slide with microscope in the foregroundRead More

What Can Electron Microscopy Do for You?

The electron microscope (EM) – where electrons, rather than photons, make the image – fell out of fashion for a while, but it has come back refreshed. Modern electron microscopes cost less, use less electricity, and are generally easier to maintain than the older models, so it is likely that you can get your hands on one. Read on to learn more about this technique, and how to implement it into your research.

Transmission electron microscopy of the nuclear envelopeRead More

Workflows and instrumentation for cryo-electron microscopy: an overview

Workflows and instrumentation for cryo-electron microscopy: an overview Now On Demand Speaker Frédéric Leroux Advanced workflow specialist, Leica Microsystems Frédéric Leroux completed his Master degree in Biology in 2007 at the University of Ghent where he gained experience in biological EM sample preparation. In 2008, he moved to the physics department at the University of…

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Microscope Disinfection: A Quick Guide

Shared microscopes have the potential to get dirty and spread nasties between samples and users. Check out our quick guide on how to clean and disinfect your microscope.

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Making a Mark: How to Set up Your Wound Healing Assay 

Are you wanting to study cell migration and need a way to quantify it? A wound healing assay is one of the earliest and simplest in-vitro assays developed to study cell migration. Collective cell migration is a characteristic behaviour seen in wound repair, cancer invasion, cancer metastasis, immune response, angiogenesis and embryonic morphogenesis. Depending on…

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How to Totally Nail Your First in situ Hybridization

It’s Monday morning and you’re about to start your first in situ hybridization (ISH). You glanced at the protocol on Friday and it seemed pretty easy. You just do a lot of washes and incubations, right? WRONG. ISH can be a nightmare. If you’re not well prepared, you’ll most likely be tearing your hair out…

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Controls for Immunofluorescence: A Beginner’s Guide 

Immunofluorescence staining is a popular and extremely powerful detection method. However, achieving publication quality immunofluorescence or fluorescent antibody staining can get tricky. It’s therefore important to ensure you have the right controls for immunofluorescence.   Since immunofluorescence staining is a long process with many steps, it is always advisable to spend some time optimizing each…

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Optimized cryo workflows using the vacuum cryo transfer system VCT500

Optimized cryo workflows using the vacuum cryo transfer system VCT500 In this tutorial, you will find: How to use the vacuum cryo transfer system The advantages of the EM VCT500 and EM VCM for different cryo workflows About their flexible integration with different analysis systems Speakers Dr. Julia KönigProduct Manager EM sample preparation for cryo-workflows…

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THUNDER Imagers: High performance, versatility and ease-of-use for your everyday imaging workflows.

THUNDER Imagers: High performance, versatility and ease-of-use for your everyday imaging workflows. In this webinar, you will learn: How to get sharp, high quality images from thick 3-dimensional microscope samples How to shorten your imaging workflow from image acquisition to data analysis A broad spectrum of imaging workflows for a range of life science applications…

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Understanding Malaria Parasite Pathogenesis by Cryo-Electron Microscopy

Understanding Malaria Parasite Pathogenesis by Cryo-Electron Microscopy In this tutorial, you will learn: The mechanisms by which the plasmodium translocon of exported proteins (PTEX)  enables the  Plasmodium parasite to take over red blood cells How single-particle cryo-electron microscopy enables us to gain insight into the PTEX molecular structure The opportunity this presents for structure-based design…

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An Introduction to Digital Images in Publications

Previously, I wrote about the different ways to use scientific illustration to communicate research. In this article, I will introduce the technical aspects of digital images and the most common image file types that scientists will encounter when preparing images for publication. Raster Versus Vector Digital images are classified based on how information is stored.…

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Basics of Fluorescence Lifetime Imaging and FLIM Applications to FRET Biosensors

Basics of Fluorescence Lifetime Imaging and FLIM Applications to FRET Biosensors In this tutorial you will learn: How researchers (Anzilotti 2019) used FLIM to elucidate the mechanisms responsible for a B cell deficiency A new approach to measure the cellular function of the Zn2+ transporter ZIP7 in primary mouse cells, comparing wild-type and CRISPR-Cas9 derived mutant cells…

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How to Weigh Molecules with Light Using Mass Photometry

How to Weigh Molecules with Light Using Mass Photometry In this tutorial, you will learn:    How mass photometry offers a universal way to study biomolecules    The strengths and limitations of mass photometry    How to use mass photometry to: accurately determine the degree of heterogeneity of a sample quantify the strength and kinetics of molecular interactions…

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The New ZEISS LSM 9 Family: Adding the Multiplex Factor to Your Confocal Imaging

The New ZEISS LSM 9 Family: Adding the Multiplex Factor to Your Confocal Imaging In this tutorial on confocal imaging, you will learn how you can: Capture weaker signals—and still get sound, reproducible data. Reach faster volumetric imaging without sacrificing resolution Increase data throughput for your imaging needs Speaker Joseph HuffSolution Manager/Application Development Engineer at…

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Cryofixation and Chemical Fixation for Electron Microscopy

Specimen preparation is the most important aspect of biological electron microscopy (EM), as it influences everything from the preservation of the sample itself to the kind of information that can be obtained. It is vital to define the questions you are asking of the sample before embarking on an electron microscopy project. This enables you…

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Ultramicrotomy for Electron Microscopy

Ultramicrotomy is the process by which a sample is cut into very thin slices or “sections”, usually for imaging by transmission electron microscopy (TEM) or relatively new techniques using scanning electron microscopy (See Array tomography in three dimensional scanning electron microscopy for biology). This technique requires a bit of finesse, and this article will help…

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