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Although the microscope is probably the most commonly used biological instrument, it is frequently used improperly. The rate-limiting step to getting high quality microscopic images is illumination of your specimen. When you examine a specimen under the microscope, the intensity and distribution of light must be clear and equal to enable you to evaluate all…
If you’re starting your PhD or post-doctoral work, chances are you’ll need to use a light microscope at some stage during your research. Some of you may be seasoned microscopists. For many of you though, this might be the first time you’ve ever plugged in a microscope, or at least the first time you’ve used…
If your immunohistochemistry is not working quite as expected, don’t fret. Check out this comprehensive guide to troubleshooting immunohistochemistry.
For a long time we’ve been able to pinpoint the subcellular location of proteins, and the advent of FISH (Fluorescence in situ Hybridization) allowed us to locate the position of genes in the nucleus, but recent advances in RNA FISH are making it easier and easier to collect the same data about individual messenger RNAs….
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