Skip to content

Microscopy and Imaging

Array Tomography for SEM 3D Reconstruction

Array Tomography for SEM 3D Reconstruction Speakers Frank Assen Robert Ranner Tutorial abstract Array tomography (AT) is a 3D image reconstruction technique for high resolution, quantitative analysis of biological structures. For optimal results, ultrathin and ordered sections are an absolute requirement. In this webinar you will get tips and tricks to optimize the workflow of…

Read More

Expanding the Limits of Electron Microscopy Sample Preparation with the Leica EM ICE High Pressure Freezer

Expanding the Limits of Electron Microscopy Sample Preparation with the Leica EM ICE High Pressure Freezer In this tutorial you will learn: Improved vitrification: Specimen vitrification without synchronisation fluid Vitrification strategies: Optimized freezing for different techniques Light and electrical stimulation: Dissect cellular processes with millisecond precision A look into the future: freezing of crystals Speakers…

Read More

Live Cell Isolation by Laser Microdissection

Live Cell Isolation by Laser Microdissection In this tutorial, you will learn: How to best prepare your specimen for live cell isolation by laser microdissection How to optimize your laser microdissection workflow How to avoid common pitfalls of this technique Speakers Dr. Oleg Podgorny Tutorial abstract Laser microdissection is a tool for the isolation of…

Read More

LAS X Navigator Tutorial: Navigating live cell imaging

LAS X Navigator Tutorial: Navigating live cell imaging Speakers Dr. Avi Jacob Tutorial abstract Join Dr. Avi Jacob as he introduces you to the Leica LAS X Navigator, a software module that makes multiparametric acquisition and data review of large data sets easier than you ever thought possible! In this tutorial, Dr. Jacobs will discuss:…

Read More

Reliable quantification of fluorescence images: from raw data to reproducible results

Reliable quantification of fluorescence images: from raw data to reproducible results In this tutorial, you will find: How to overcome issues with stage drift and crosstalk between channels How to obtain reliable images and reproducible quantification results How to proceed with advanced image analysis after restoring images Speakers Vincent SchoonderwoerSenior Imaging/Application Specialist, SVI Tutorial abstract…

Read More

Super-resolved STED spectroscopy: New insights into molecular membrane dynamics

Super-resolved STED spectroscopy: New insights into molecular membrane dynamics Speakers Christian Eggeling Tutorial abstract Molecular interactions are key in cellular signalling. They are often ruled or rendered by the mobility of the involved molecules. We present different tools that are able to determine such mobility and potentially extract interaction dynamics. Specifically, the direct and non-invasive…

Read More

An Introduction to Cardiac Optical Mapping

What Is Optical Mapping and How Is It Used? Synchronisation of the contraction of heart muscle is essential for the efficient pumping of blood through the circulatory system. Cardiac contraction is controlled by the regulated spread of electrical impulses from cell-to-cell within the heart.  In pathological conditions, these electrical impulses can become disordered and lead…

Read More

Precise and lightning-fast spectral Fluorescence Lifetime Imaging at video rate integrated in a high-end confocal microscope

Precise and lightning-fast spectral Fluorescence Lifetime Imaging at video rate integrated in a high-end confocal microscope Speakers Kees JalinkProfessor, The Netherlands Cancer Institute Tutorial abstract Förster (Fluorescence) Resonance Energy Transfer (FRET) has become a powerful tool to study protein-protein interactions and signal transduction in living cells. FRET is commonly read out either by detecting the…

Read More

Protein Colocalization: You Know They Overlap, but Can You Prove It?

Microscopy by default is a technique that allows us to observe, rather than measure, biological events and make conclusions based on what we see, rather than based on some calculations. The topic of this article may therefore seem a little surprising since it deals with the measurements of colocalization. While there are situations where you…

Read More

Your guide to Complete and Fast 3D Image Analysis in Microscopy

Your guide to Complete and Fast 3D Image Analysis in Microscopy Join Dr. Chris Zugates as he takes you through a typical workflow of image analysis, learn how to easily process your data and find the objects of interest quickly and interactively creating meaningful results for publication.In this tutorial, you will find: How to handle…

Read More

Automated Image Analysis – the Future of Data Acquisition?

Automated image analysis uses finely tuned software to extract data from digital images. Algorithms recognize specific shapes and patterns in the images and gather quantitative information that is then used for further data analysis. The pharmaceutical and biological research industries have benefitted greatly from this technology, which allows researchers to analyze hundreds—if not thousands—of samples…

Read More

Safranin: Cheap Stain to Visualize Chromosomes

As an undergraduate student, one of the first experiments I did was staining chromosomes in mitotically active onion root tip cells. The stains that are conventionally used for this purpose are acetocarmine or aceto-orcein (which smell like vinegar). However, the cost of these stains is quite high. Personally, I find safranin, which is another stain, more…

Read More

Get the Best Out of Your Microscopy Images with Huygens Batch Express

Get the Best Out of Your Microscopy Images with Huygens Batch Express In this tutorial, you will learn about the advantages of deconvolving all your images fully automatically.In this tutorial, you will find: How to improve the quality of your images with the new Batch Express How to increase resolution and signal of all your…

Read More

Get Your Microscopy Mojo Back with Our Image Acquisition and Processing Tips

Ever since the invention of the first compound microscope by Zacharias Jansen in 1590, our understanding of the microscopic world has grown exponentially. Microscopes have evolved from mere assemblies of magnifying lenses to extremely powerful tools for visualization on the atomic scale. You can find a wealth of information on the workings of a microscope…

Read More

Color Transmission Electron Microscopy

There are two types of electron microscopy—transmission electron microscopy (TEM) and scanning electron microscopy (SEM). SEM creates fascinating 2D images by bouncing electrons off the surface of the sample. I highly recommend searching for SEM samples on Google images. There are a wide variety of applications for electron microscopy. While SEM images are aesthetically amazing,…

Read More

New 2D Superresolution mode for ZEISS Airyscan – Fast and gentle confocal imaging with 120 nm resolution

New 2D Superresolution mode for ZEISS Airyscan – Fast and gentle confocal imaging with 120 nm resolution Speakers Joseph Huff, PhD Tutorial abstract Utilizing a pinhole-plane imaging concept, ZEISS Airyscan allows for simultaneous improvement in resolution and signal-to-noise by capitalizing on an innovative 32-channel GaAsP photomultiplier tube (PMT) array detector. Each detection channel functions as…

Read More

How to Conduct Localized Proteomics of Microscopic Regions

Precise and lightning-fast spectral Fluorescence Lifetime Imaging at video rate integrated in a high-end confocal microscope In this tutorial, you will learn: How to perform isolated proteomics of microscopic regions of interest How to use this technique with FFPE tissue How proteomics can help you understand molecular mechanisms in disease Speakers Dr. Eleanor Drummond Tutorial abstract…

Read More

From Cells to Scope: Chamber Slide Immunochemistry

Immunolabeling is the tried-and-true immunochemistry method of getting the stain you want onto the molecular target you want. Whether that target is contained within a large region of tissue (immunohistochemistry) or inside a single cell (immunocytochemistry), the ability to accurately label large numbers of samples will simplify your workflow and help you to achieve excellent…

Read More

Lasers for Confocal Microscopy: Part II

Continuing from our first article on lasers for confocal microscopy, we will now discuss two specialized types of lasers: lasers for two-photon excitation and tunable, white light lasers. We will also discuss the applications of the two lasers. Lasers for Two-Photon Excitation The two-photon absorption phenomenon was first described for microscopy in 1931. Here, the…

Read More

Things to Consider When Buying a Microscope Camera (Part 2)

In the first part of this series, we discussed the differences between a color and a monochrome microscope camera and when one is advantageous over the other. We also touched on the subject of optimal camera resolution for a given imaging system. In this part, we will tackle a few additional camera specifications and how…

Read More

Biological Applications of X-Ray Microscopy and Correlative XRM – FIB-SEM Imaging

In this webinar, you will learn about the power of 3D imaging of larger samples.  In particular, you will: Gain insight in to how to use x-ray microscopy to analyze biological samples Learn the basics about sample preparation for x-ray microscopy Understand the benefits and drawbacks to different imaging conditions X-Ray Microscopy (XRM) is a…

Read More

Large Volume Serial Blockface Electron Microscopy Imaging

Large Volume Serial Blockface Electron Microscopy Imaging An unlikely renaissance is occurring in biological imaging, propelled by the ongoing development of serial blockface electron microscopy imaging technologies. Imaging systems based on scanning EM instruments place large-volume automated EM serial imaging within reach of most labs.  How best to take advantage of the automation and versatility?…

Read More

Things to Consider When Buying a Microscope Camera (Part 1)

Purchasing a microscope camera is one of the most daunting tasks you might have to undertake. Before you set out to buy that camera, carefully consider your applications. Things like sample brightness or the speed of the phenomenon you are trying to capture can dictate your choices. Also, this is the time to make peace…

Read More

All You Need to Know (and More) About Embedding for Electron Microscopy

Interested in the detailed structure of your tissue? High-resolution imaging techniques, such as brain electron microscopy, provide an intricate view of your tissue. While it may be a rather complicated procedure with nasty chemicals, the advantages of epon embedding can make it the best choice for morphological studies. The hard blocks are excellent for structural…

Read More

Tissue Embedding Throwdown: Paraffin vs OCT vs Resin

Tissue embedding and sectioning is a backbone of many biological research labs. While commiserating with other grad students over tedious hours spent in the lab, you’re probably aware that there is more than one way to slice up a chunk of tissue. We’ve previously introduced what to consider when choosing a tissue embedding medium and…

Read More

How to Use CellProfiler for Cell Imaging

Are you trying to figure out how to calculate intensities of fluorescently-labeled single cells? Do you have cells at high densities or present in clusters? Are you worried that your current cell imaging analysis software is unable to mark clear boundaries around each cell in a cell cluster? Don’t fear, because CellProfiler 2.1 is here to…

Read More

Optocardiography: Optical Imaging of Cardiac Physiology

In this webinar, you will learn how to use optical imaging for functional cardiac physiological mapping of transmembrane potential, calcium handling, and metabolism. The main aspects covered in the webinar include: How to design an effective optocardiography system and select optimal fluorescence probes for your research needs using open source hardware and software. Troubleshooting tips…

Read More

Unpacking the Daunting Task of Stereology for Electron Microscopy

Unpacking the Daunting Task of Stereology for Electron Microscopy Electron microscopy provides fantastic detail and resolution, with brain electron microscopy allowing visualisation of neurons and their individual synaptic connections. You may find yourself needing to count these neurons or connections, which can easily go into the billions. But counting these one-by-one isn’t really feasible. This…

Read More

Detecting Cell Apoptosis on Tissue Slides

Apoptosis, or programmed cell death, is a physiological process in which individual cells are set to die without harming their environment. It involves a cascade of complex and tightly regulated cellular events. Detecting apoptosis on tissue slides, involves either detecting the molecular participants of these events, or the morphological changes that occur on the cellular…

Read More

Mouse Whole-Brain Volume Electron Microscopy for Cellular Connectomics – Enabling Large-Scale SEM Projects with ZEISS MultiSEM

Recent advances in high-throughput multi-beam scanning electron microscopy (EM) and mouse whole-brain EM preparation and collection on tape (“Brain-on-Tape”) have resulted in substantial progress towards a nano-scale map of the whole mouse brain. These maps can be used to determine how individual neurons are synaptically connected and can be used to reconstruct the precise “wiring…

Read More

Enhance Your Imaging with Super-Resolution: Improve Spatial Resolution and Increase Signal to Noise Ratio in Difficult Samples

In these informative case studies, you will learn how: HyVolution 2 can improve the spatial resolution you get from your sample, whilst remaining gentle to the fluorophores HyVolution 2 overcomes issues experienced with other imaging modalities, even in difficult samples such as plant material, increasing resolution and signal to noise ratio, thereby facilitating downstream image…

Read More

Harnessing the Power of Heterogeneity in Cryo-electron Microscopy

A major impediment in the determination of high resolution protein structures by single particle cryo-electron microscopy has been the presence of sample heterogeneity. Oftentimes, heterogeneity is due the dynamic nature of protein complexes, which can exist in multiple different conformational states in solution. Recent advancements in cryo-electron image processing have provided tools to accurately sort…

Read More

Automated Microscopy

The traditional microscope that you know and love is operated manually. Picture the scene: the microscopist chooses the light source, gently places the sample the moveable stage, selects the objective lens, and scans to select the field of view. This process is perfect for processing and analyzing a small number of samples per day. But…

Read More

Revealing Cellular Dynamics with Millisecond Precision – The New Tool That Turned Electron Micrographs into Motion Pictures of Neural Communication

Revealing Cellular Dynamics with Millisecond Precision – The New Tool That Turned Electron Micrographs into Motion Pictures of Neural Communication In this tutorial: What if you can dissect the cellular dynamics with millisecond precision? What if you can unravel the morphological transformation of a neuron millisecond by millisecond using electron microscopy? Could this be even…

Read More

In Situ Zymography: Let’s Catch that Enzyme in Action!

In situ zymography (ISZ) is the best choice to study proteases. Proteases are a challenge to study as proteases are extremely potent enzymes. As such, they need to be controlled at multiple levels to prevent them from being unleashed and making a cellular mess. Regulation of their activity occurs at virtually all levels: transcriptional control,…

Read More

Automated Cell Counting with a Fluorescent Twist

Cell counting is the bane of existence of many researchers. Countless hours spent in front of the microscope with a haemocytometer on the stand and a manual tally (or “clicker”) in hand can be really daunting. Not to mention that no one will ever double check your count if you don’t take a picture. Those…

Read More
Scroll To Top