Microscopy and Imaging
Workflows and instrumentation for cryo-electron microscopy: an overview
Workflows and instrumentation for cryo-electron microscopy: an overview Now On Demand Speaker Frédéric Leroux Advanced workflow specialist, Leica Microsystems Frédéric Leroux completed his Master degree in Biology in 2007 at the University of Ghent where he gained experience in biological EM sample preparation. In 2008, he moved to the physics department at the University of…
Read MoreMicroscope Disinfection: A Quick Guide
Shared microscopes have the potential to get dirty and spread nasties between samples and users. Check out our quick guide on how to clean and disinfect your microscope.
Read MoreImaging and Analyzing Your Wound Healing Assay
Don’t be daunted by imaging and analyzing your wound healing assay; our top tips will get it looking perfect.
Read MoreMaking a Mark: How to Set up Your Wound Healing Assay
Wound healing assay is one of the earliest and simplest in-vitro assays for studying cell migration. Discover if this assay is right for you and learn how to set it up to get reproducible data.
Read MoreHow to Totally Nail Your First in situ Hybridization
It’s Monday morning and you’re about to start your first in situ hybridization (ISH). You glanced at the protocol on Friday and it seemed pretty easy. You just do a lot of washes and incubations, right? WRONG. ISH can be a nightmare. If you’re not well prepared, you’ll most likely be tearing your hair out…
Read MoreControls for Immunofluorescence: A Beginner’s Guide
Achieving publication-quality immunofluorescence images can get tricky and it’s therefore important to ensure you have the right controls for immunofluorescence.
Read MoreOptimized cryo workflows using the vacuum cryo transfer system VCT500
Optimized cryo workflows using the vacuum cryo transfer system VCT500 In this tutorial, you will find: How to use the vacuum cryo transfer system The advantages of the EM VCT500 and EM VCM for different cryo workflows About their flexible integration with different analysis systems Speakers Dr. Julia KönigProduct Manager EM sample preparation for cryo-workflows…
Read MoreTHUNDER Imagers: High performance, versatility and ease-of-use for your everyday imaging workflows.
THUNDER Imagers: High performance, versatility and ease-of-use for your everyday imaging workflows. In this webinar, you will learn: How to get sharp, high quality images from thick 3-dimensional microscope samples How to shorten your imaging workflow from image acquisition to data analysis A broad spectrum of imaging workflows for a range of life science applications…
Read MoreUnderstanding Malaria Parasite Pathogenesis by Cryo-Electron Microscopy
Understanding Malaria Parasite Pathogenesis by Cryo-Electron Microscopy In this tutorial, you will learn: The mechanisms by which the plasmodium translocon of exported proteins (PTEX) enables the Plasmodium parasite to take over red blood cells How single-particle cryo-electron microscopy enables us to gain insight into the PTEX molecular structure The opportunity this presents for structure-based design…
Read MoreAn Introduction to Digital Images in Publications
Previously, I wrote about the different ways to use scientific illustration to communicate research. In this article, I will introduce the technical aspects of digital images and the most common image file types that scientists will encounter when preparing images for publication. Raster Versus Vector Digital images are classified based on how information is stored.…
Read MoreBasics of Fluorescence Lifetime Imaging and FLIM Applications to FRET Biosensors
Basics of Fluorescence Lifetime Imaging and FLIM Applications to FRET Biosensors In this tutorial you will learn: How researchers (Anzilotti 2019) used FLIM to elucidate the mechanisms responsible for a B cell deficiency A new approach to measure the cellular function of the Zn2+ transporter ZIP7 in primary mouse cells, comparing wild-type and CRISPR-Cas9 derived mutant cells…
Read MoreHow to Weigh Molecules with Light Using Mass Photometry
How to Weigh Molecules with Light Using Mass Photometry In this tutorial, you will learn: How mass photometry offers a universal way to study biomolecules The strengths and limitations of mass photometry How to use mass photometry to: accurately determine the degree of heterogeneity of a sample quantify the strength and kinetics of molecular interactions…
Read MoreThe New ZEISS LSM 9 Family: Adding the Multiplex Factor to Your Confocal Imaging
The New ZEISS LSM 9 Family: Adding the Multiplex Factor to Your Confocal Imaging In this tutorial on confocal imaging, you will learn how you can: Capture weaker signals—and still get sound, reproducible data. Reach faster volumetric imaging without sacrificing resolution Increase data throughput for your imaging needs Speaker Joseph HuffSolution Manager/Application Development Engineer at…
Read MoreCryofixation and Chemical Fixation for Electron Microscopy
Sample fixation for electron microscopy can be done using either chemical fixation or cryofixation. Discover the benefits and drawbacks of each of these methods.
Read MoreUltramicrotomy for Electron Microscopy
Ultramicrotomy is the process by which a sample is cut into very thin slices or “sections”, usually for imaging by transmission electron microscopy (TEM) or relatively new techniques using scanning electron microscopy (See Array tomography in three dimensional scanning electron microscopy for biology). This technique requires a bit of finesse, and this article will help…
Read MoreMicroscope Finder Slides: Their History, Development, and Use
Have you ever been looking through a box of slides and found something that you want to image or look at later, or even show to one of your colleagues or supervisor? Finding that exact spot on the slide at a later date can prove to be difficult- using a marker pen on the coverslip…
Read MoreCryo-electron Tomography for Cell Biology
Cryo-electron Tomography for Cell Biology In this tutorial, you will find: The complete workflow for in situ cryo-electron tomography How subtomogram averaging within the cell yields native-state structures of macromolecular complexes (e.g., the asymmetric and dilated nuclear pore of algae) How mapping these structures back into the native cellular environment reveals new molecular interactions that are only…
Read MoreCryo-Electron Microscopy and the Complexity of Cancer
Cryo-Electron Microscopy and the Complexity of Cancer Shed light on disease with an electron beam, one molecule at a timeIn this tutorial, you will find: How structural biology can change the face of your research The biological challenges of cancer research Advantages of cryo-EM for your research Speaker Simon Poepsel Tutorial abstract Covering both biology…
Read MoreRevealing the fastest processes in superresolution – with Lattice SIM and ZEISS Elyra 7
Revealing the fastest processes in superresolution – with Lattice SIM and ZEISS Elyra 7 Speakers Renée M. Dalrymple Tutorial abstract Your life sciences research often requires you to measure, quantify and understand the finest details and sub-cellular structures of your sample. You may be working with tissue, bacteria, organoids, neurons, living or fixed -cells and…
Read MoreLaser in a droplet
When you hear about a laser, you likely imagine a medium-size apparatus with a light beam coming out of it, not a bacterium in a drop of liquid. Well, Turkish and British scientists went beyond ordinary imagination – they expressed a fluorescent protein in E.coli and suspended live bacteria in droplets. Illuminated droplets served as…
Read MoreThinking Outside the Box: Microscopy for Immunologists
We explore microscopy for immunologists and bring them into the light and away from the 96-well plate, at least for some of the time.
Read MoreArray Tomography for SEM 3D Reconstruction
Array Tomography for SEM 3D Reconstruction Speakers Frank Assen Robert Ranner Tutorial abstract Array tomography (AT) is a 3D image reconstruction technique for high resolution, quantitative analysis of biological structures. For optimal results, ultrathin and ordered sections are an absolute requirement. In this webinar you will get tips and tricks to optimize the workflow of…
Read MoreExpanding the Limits of Electron Microscopy Sample Preparation with the Leica EM ICE High Pressure Freezer
Expanding the Limits of Electron Microscopy Sample Preparation with the Leica EM ICE High Pressure Freezer In this tutorial you will learn: Improved vitrification: Specimen vitrification without synchronisation fluid Vitrification strategies: Optimized freezing for different techniques Light and electrical stimulation: Dissect cellular processes with millisecond precision A look into the future: freezing of crystals Speakers…
Read MoreLive Cell Isolation by Laser Microdissection
Live Cell Isolation by Laser Microdissection In this tutorial, you will learn: How to best prepare your specimen for live cell isolation by laser microdissection How to optimize your laser microdissection workflow How to avoid common pitfalls of this technique Speakers Dr. Oleg Podgorny Tutorial abstract Laser microdissection is a tool for the isolation of…
Read MoreLAS X Navigator Tutorial: Navigating live cell imaging
LAS X Navigator Tutorial: Navigating live cell imaging Speakers Dr. Avi Jacob Tutorial abstract Join Dr. Avi Jacob as he introduces you to the Leica LAS X Navigator, a software module that makes multiparametric acquisition and data review of large data sets easier than you ever thought possible! In this tutorial, Dr. Jacobs will discuss:…
Read MoreReliable quantification of fluorescence images: from raw data to reproducible results
Reliable quantification of fluorescence images: from raw data to reproducible results In this tutorial, you will find: How to overcome issues with stage drift and crosstalk between channels How to obtain reliable images and reproducible quantification results How to proceed with advanced image analysis after restoring images Speakers Vincent SchoonderwoerSenior Imaging/Application Specialist, SVI Tutorial abstract…
Read MoreSuper-resolved STED spectroscopy: New insights into molecular membrane dynamics
Super-resolved STED spectroscopy: New insights into molecular membrane dynamics Speakers Christian Eggeling Tutorial abstract Molecular interactions are key in cellular signalling. They are often ruled or rendered by the mobility of the involved molecules. We present different tools that are able to determine such mobility and potentially extract interaction dynamics. Specifically, the direct and non-invasive…
Read MoreAn Introduction to Cardiac Optical Mapping
What Is Optical Mapping and How Is It Used? Synchronisation of the contraction of heart muscle is essential for the efficient pumping of blood through the circulatory system. Cardiac contraction is controlled by the regulated spread of electrical impulses from cell-to-cell within the heart. In pathological conditions, these electrical impulses can become disordered and lead…
Read MoreNew-ISH on the Block: Introduction to RNAscope®
RNAscope is a new method of quantitative RNA in situ-hybridization that has taken laboratories by storm. Learn advantages over traditional techniques and how RNAscope works in this introduction article.
Read MorePrecise and lightning-fast spectral Fluorescence Lifetime Imaging at video rate integrated in a high-end confocal microscope
Precise and lightning-fast spectral Fluorescence Lifetime Imaging at video rate integrated in a high-end confocal microscope Speakers Kees JalinkProfessor, The Netherlands Cancer Institute Tutorial abstract Förster (Fluorescence) Resonance Energy Transfer (FRET) has become a powerful tool to study protein-protein interactions and signal transduction in living cells. FRET is commonly read out either by detecting the…
Read MoreYour guide to Complete and Fast 3D Image Analysis in Microscopy
Your guide to Complete and Fast 3D Image Analysis in Microscopy Join Dr. Chris Zugates as he takes you through a typical workflow of image analysis, learn how to easily process your data and find the objects of interest quickly and interactively creating meaningful results for publication.In this tutorial, you will find: How to handle…
Read MoreAutomated Image Analysis – the Future of Data Acquisition?
Automated image analysis uses finely tuned software to extract data from digital images. Algorithms recognize specific shapes and patterns in the images and gather quantitative information that is then used for further data analysis. The pharmaceutical and biological research industries have benefitted greatly from this technology, which allows researchers to analyze hundreds—if not thousands—of samples…
Read MoreSafranin: Cheap Stain to Visualize Chromosomes
As an undergraduate student, one of the first experiments I did was staining chromosomes in mitotically active onion root tip cells. The stains that are conventionally used for this purpose are acetocarmine or aceto-orcein (which smell like vinegar). However, the cost of these stains is quite high. Personally, I find safranin, which is another stain, more…
Read MoreGet the Best Out of Your Microscopy Images with Huygens Batch Express
Get the Best Out of Your Microscopy Images with Huygens Batch Express In this tutorial, you will learn about the advantages of deconvolving all your images fully automatically.In this tutorial, you will find: How to improve the quality of your images with the new Batch Express How to increase resolution and signal of all your…
Read MoreGet Your Microscopy Mojo Back with Our Image Acquisition and Processing Tips
Ever since the invention of the first compound microscope by Zacharias Jansen in 1590, our understanding of the microscopic world has grown exponentially. Microscopes have evolved from mere assemblies of magnifying lenses to extremely powerful tools for visualization on the atomic scale. You can find a wealth of information on the workings of a microscope…
Read MoreColor Transmission Electron Microscopy
There are two types of electron microscopy—transmission electron microscopy (TEM) and scanning electron microscopy (SEM). SEM creates fascinating 2D images by bouncing electrons off the surface of the sample. I highly recommend searching for SEM samples on Google images. There are a wide variety of applications for electron microscopy. While SEM images are aesthetically amazing,…
Read MoreNew 2D Superresolution mode for ZEISS Airyscan – Fast and gentle confocal imaging with 120 nm resolution
New 2D Superresolution mode for ZEISS Airyscan – Fast and gentle confocal imaging with 120 nm resolution Speakers Joseph Huff, PhD Tutorial abstract Utilizing a pinhole-plane imaging concept, ZEISS Airyscan allows for simultaneous improvement in resolution and signal-to-noise by capitalizing on an innovative 32-channel GaAsP photomultiplier tube (PMT) array detector. Each detection channel functions as…
Read MoreHow to Conduct Localized Proteomics of Microscopic Regions
Precise and lightning-fast spectral Fluorescence Lifetime Imaging at video rate integrated in a high-end confocal microscope In this tutorial, you will learn: How to perform isolated proteomics of microscopic regions of interest How to use this technique with FFPE tissue How proteomics can help you understand molecular mechanisms in disease Speakers Dr. Eleanor Drummond Tutorial abstract…
Read MoreFrom Cells to Scope: Chamber Slide Immunochemistry
Immunolabeling is the tried-and-true immunochemistry method of getting the stain you want onto the molecular target you want. Whether that target is contained within a large region of tissue (immunohistochemistry) or inside a single cell (immunocytochemistry), the ability to accurately label large numbers of samples will simplify your workflow and help you to achieve excellent…
Read MoreLasers for Confocal Microscopy: Part II
Continuing from our first article on lasers for confocal microscopy, we will now discuss two specialized types of lasers: lasers for two-photon excitation and tunable, white light lasers. We will also discuss the applications of the two lasers. Lasers for Two-Photon Excitation The two-photon absorption phenomenon was first described for microscopy in 1931. Here, the…
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