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Cloning and Expression

Understanding and Designing Flanking Homology DNA Assembly Experiments

Understanding and Designing Flanking Homology DNA Assembly Experiments Join us in this webinar on demystifying DNA assembly.In this tutorial, you will find: How flanking homology DNA assembly methods work How to use web-based software to design experimental methods for flanking homology DNA assembly methods How synthetic DNA fragments fit in to the DNA assembly process…

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Optimize Bacterial Protein Expression by Considering these 4 Variables

So, you have successfully cloned your gene of interest and are eager to purify buckets of protein. No matter your eventual application—kinetic experiments using a SPR instrument, structural analysis using X-ray crystallography, or any other experiment—you’ll need to express your protein first.  Now, it’s time to put your expression plasmid into E. coli and get…

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