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Are you preparing to run your first column chromatography experiment? Here are some basics that you should know before you use that precious sample.
Using ethanol precipitation to isolate or concentrate your nucleic acids? Find out how this routinely used technique works, and get tips to produce the best results.
Tissue processing for histology is a key step between fixation and embedding. We take you through the steps of tissue processing in this simple guide.
Nucleic acid extraction kits are routinely used in today’s molecular biology labs. Read on to learn more about what is inside these black boxes of wonder and how you can get the best results for your preps.
Keep your lab’s freeze dryer running smoothly with our expert tips on care and maintenance, including oil changes and vacuum leak fixes.
In-cell Westerns are a powerful technique that has enhanced how researchers analyze protein expression levels and signaling pathways within fixed cells. Learn about their primary advantages, applications, and some of the best tech and products to perform them.
Most experiments start with a piece of DNA—either plasmid DNA or genomic DNA. And your downstream uses for it dictate how much you need, what contaminants you can tolerate, and your extraction and purification methods. In this article, we explain the key differences between plasmid and genomic DNA extraction methods.
So you’ve got your flow cytometry training booked and are one step closer to that precious data. Read our 7 top tips on how to get the most from your flow cytometry training.
Dive into the world of protein structure science, where AI is revolutionizing the field by offering accurate predictions and free, open-access tools. In this beginner’s guide, we’ll explore these groundbreaking tools, discuss their strengths and weaknesses, and examine the implications for traditional experimental methods like NMR and X-ray crystallography.
Don’t let centrifugation scare you. Learn how to balance properly, when to use the brake, and what the difference is between RCF and RPM!
What is protein crystallography? Whether you are about to enter the world of protein crystallography or work with a protein crystallographer on your grant, this article will be useful.
Discover how Ponceau S works, how to perform staining, and get a detailed guide to interpreting what the staining means when troubleshooting your failed blots.
Whether you want to get started with fluorescence microscopy or already use it, this guide will ensure you know the basics and get the best out of your fluorescence microscopy.
ECL is an expensive reagent. Why not learn how to make ECL yourself? This cheap and simple option will give you better blots more often!
Need to get a handle on the fundamental differences between HPLC and GC to pick one for your studies? Here’s a quick explainer!
Do you fully understand why enzymes have the best catalytic activity within a specific temperature ranges? Find out in our handy guide.
Do you need to learn about gas chromatography? This article takes you through the basic principles and instrumentation. With illustrations!
Not sure what FRET is, or just need a refresher on how FRET works? Read our short guide to understand the usefulness of FRET for studying protein-protein interactions.
Buffers provide a stable chemical environment for all our experiments. But how do buffers work? And how do you pick one? Read on to find out.
In biosciences, we purify our target molecules using chromatography. So, we need a thorough understanding of how chromatography works!
A neglected pH meter means less reliable experiments, poor reproducibility, and your time wasted. So, learn how to use a pH meter correctly!
Understanding the basic (and simple!) chemistry behind DNA ligation will help you get better DNA ligation results. Learn all about it here.
NMR is a structural tool that plays second fiddle to crystallography. But there’s a cheap, label-free method of investigating protein folding by NMR.
We all rely on pH, pKa, and pI for a vast amount of our research. But what is pH? How does it relate to pKa and pI? Read the definitive guide.
SDS-PAGE is the technique that unites life scientists. We all perform it during our research to separate protein analytes and, therefore, we all need a good SDS-PAGE gel recipe.
Do you want to know about a cool way to detect and tell the difference between virus particles? Then read on to discover flow virometry!
Protease inhibitors are a requirement in many lab experiments. In this article, we’ll take you through how protease inhibitors work, why we need them, and how to use them correctly and safely.
Laemmli buffer is used almost every day by life since researchers. But what do all those components actually do? Find out in this handy article
Understanding how DNA extraction kits work is the key to troubleshooting your extraction issues.
Phenol extraction is a common method for removing proteins from nucleic acids. Discover how phenol extraction of DNA works.
Discover how the luciferase reporter assay works and how to get starting using it in your research.
Want to use Gateway cloning or having trouble using this technology? Find out how it works and get helpful tips to increase your success.
Knowing how SDS-PAGE works means that you can troubleshoot any issues in your experiment and tweak the setup to get publication-worthy figures. Find out how it works here.
Alkaline lysis for plasmid isolation? That’s like the ABCs in a molecular biology lab. Read this detailed article to understand the process behind this common technique.
CRISPR interference allows for the regulation of gene expression in vivo. Here’s a short guide to how it works.
If you need to copy, sequence, or quantify DNA, you need to know about PCR. Read our guide to the PCR process, and discover tips to help you avoid the most common PCR pitfalls.
I was so excited to start using 384-well plates for my assays. With so many wells, these plates are useful for testing many conditions in parallel, as required in ELISAs, siRNA library screens, and drug treatment dilutions. However, I quickly learned that pipetting in these plates is more complicated than I thought. This article contains…
Whether you work with human cell lines or microbes, their growth is governed by the same principles. I invite you to learn about something that lies at the base of any work with cell culture, whether cells have circular or linear chromosomes: the S-curve of the population growth. The length of each phase depends on…
Purchasing a microscope camera is one of the most daunting tasks you might have to undertake. Before you set out to buy that camera, carefully consider your applications. Things like sample brightness or the speed of the phenomenon you are trying to capture can dictate your choices. Also, this is the time to make peace…
Fluorescence is one of the most important and useful tools in a biologist’s toolbox. In biology, nearly every field, from physiology to immunology, uses fluorescent molecules (aka fluorophores) to detect proteins. However, the specific science behind how fluorescence works can be confusing or overlooked. Have no fear! In this article, we break down key points of…
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