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Microscopy and Imaging

Making a Mark: How to Set up Your Wound Healing Assay 

Are you wanting to study cell migration and need a way to quantify it? A wound healing assay is one of the earliest and simplest in-vitro assays developed to study cell migration. Collective cell migration is a characteristic behaviour seen in wound repair, cancer invasion, cancer metastasis, immune response, angiogenesis and embryonic morphogenesis. Depending on…

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How to Totally Nail Your First in situ Hybridization

It’s Monday morning and you’re about to start your first in situ hybridization (ISH). You glanced at the protocol on Friday and it seemed pretty easy. You just do a lot of washes and incubations, right? WRONG. ISH can be a nightmare. If you’re not well prepared, you’ll most likely be tearing your hair out…

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Controls for Immunofluorescence: A Beginner’s Guide 

Immunofluorescence staining is a popular and extremely powerful detection method. However, achieving publication quality immunofluorescence or fluorescent antibody staining can get tricky. It’s therefore important to ensure you have the right controls for immunofluorescence.   Since immunofluorescence staining is a long process with many steps, it is always advisable to spend some time optimizing each…

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Optimized cryo workflows using the vacuum cryo transfer system VCT500

Optimized cryo workflows using the vacuum cryo transfer system VCT500 In this tutorial, you will find: How to use the vacuum cryo transfer system The advantages of the EM VCT500 and EM VCM for different cryo workflows About their flexible integration with different analysis systems Speakers Dr. Julia KönigProduct Manager EM sample preparation for cryo-workflows…

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THUNDER Imagers: High performance, versatility and ease-of-use for your everyday imaging workflows.

THUNDER Imagers: High performance, versatility and ease-of-use for your everyday imaging workflows. In this webinar, you will learn: How to get sharp, high quality images from thick 3-dimensional microscope samples How to shorten your imaging workflow from image acquisition to data analysis A broad spectrum of imaging workflows for a range of life science applications…

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Understanding Malaria Parasite Pathogenesis by Cryo-Electron Microscopy

Understanding Malaria Parasite Pathogenesis by Cryo-Electron Microscopy In this tutorial, you will learn: The mechanisms by which the plasmodium translocon of exported proteins (PTEX)  enables the  Plasmodium parasite to take over red blood cells How single-particle cryo-electron microscopy enables us to gain insight into the PTEX molecular structure The opportunity this presents for structure-based design…

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How History Shaped Modern Optical Microscopes, Part One: Simple and Compound Microscopes

Having some knowledge of microscope history can be beneficial to understanding and appreciating specific configurations and components, and how they produce an optimal image. For example, practically all laboratory microscopes use achromatic objectives to partly compensate for chromatic aberration. For high-quality imaging, fluorescence microscopy, and photomicroscopy, fluorite and apochromatic objectives are used to largely overcome…

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An Introduction to Digital Images in Publications

Previously, I wrote about the different ways to use scientific illustration to communicate research. In this article, I will introduce the technical aspects of digital images and the most common image file types that scientists will encounter when preparing images for publication. Raster Versus Vector Digital images are classified based on how information is stored.…

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Basics of Fluorescence Lifetime Imaging and FLIM Applications to FRET Biosensors

Basics of Fluorescence Lifetime Imaging and FLIM Applications to FRET Biosensors In this tutorial you will learn: How researchers (Anzilotti 2019) used FLIM to elucidate the mechanisms responsible for a B cell deficiency A new approach to measure the cellular function of the Zn2+ transporter ZIP7 in primary mouse cells, comparing wild-type and CRISPR-Cas9 derived mutant cells…

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How to Weigh Molecules with Light Using Mass Photometry

How to Weigh Molecules with Light Using Mass Photometry In this tutorial, you will learn:    How mass photometry offers a universal way to study biomolecules    The strengths and limitations of mass photometry    How to use mass photometry to: accurately determine the degree of heterogeneity of a sample quantify the strength and kinetics of molecular interactions…

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The New ZEISS LSM 9 Family: Adding the Multiplex Factor to Your Confocal Imaging

The New ZEISS LSM 9 Family: Adding the Multiplex Factor to Your Confocal Imaging In this tutorial on confocal imaging, you will learn how you can: Capture weaker signals—and still get sound, reproducible data. Reach faster volumetric imaging without sacrificing resolution Increase data throughput for your imaging needs Speaker Joseph HuffSolution Manager/Application Development Engineer at…

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Cryofixation and Chemical Fixation for Electron Microscopy

Specimen preparation is the most important aspect of biological electron microscopy (EM), as it influences everything from the preservation of the sample itself to the kind of information that can be obtained. It is vital to define the questions you are asking of the sample before embarking on an electron microscopy project. This enables you…

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Ultramicrotomy for Electron Microscopy

Ultramicrotomy is the process by which a sample is cut into very thin slices or “sections”, usually for imaging by transmission electron microscopy (TEM) or relatively new techniques using scanning electron microscopy (See Array tomography in three dimensional scanning electron microscopy for biology). This technique requires a bit of finesse, and this article will help…

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Microscope Finder Slides: Their History, Development, and Use

Have you ever been looking through a box of slides and found something that you want to image or look at later, or even show to one of your colleagues or supervisor? Finding that exact spot on the slide at a later date can prove to be difficult- using a marker pen on the coverslip…

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Cryo-electron Tomography for Cell Biology

Cryo-electron Tomography for Cell Biology In this tutorial, you will find: The complete workflow for in situ cryo-electron tomography How subtomogram averaging within the cell yields native-state structures of macromolecular complexes (e.g., the asymmetric and dilated nuclear pore of algae) How mapping these structures back into the native cellular environment reveals new molecular interactions that are only…

CryoEM image of Golgi apparatusRead More

Cryo-Electron Microscopy and the Complexity of Cancer

Cryo-Electron Microscopy and the Complexity of Cancer Shed light on disease with an electron beam, one molecule at a timeIn this tutorial, you will find: How structural biology can change the face of your research The biological challenges of cancer research Advantages of cryo-EM for your research Speaker Simon Poepsel Tutorial abstract Covering both biology…

PRC2Read More

Revealing the fastest processes in superresolution – with Lattice SIM and ZEISS Elyra 7

Revealing the fastest processes in superresolution – with Lattice SIM and ZEISS Elyra 7 Speakers Renée M. Dalrymple Tutorial abstract Your life sciences research often requires you to measure, quantify and understand the finest details and sub-cellular structures of your sample. You may be working with tissue, bacteria, organoids, neurons, living or fixed -cells and…

alpha-tubulin labeled with Alexa 647Read More

Laser in a droplet

When you hear about a laser, you likely imagine a medium-size apparatus with a light beam coming out of it, not a bacterium in a drop of liquid. Well, Turkish and British scientists went beyond ordinary imagination – they expressed a fluorescent protein in E.coli and suspended live bacteria in droplets. Illuminated droplets served as…

Water dropletsRead More

Optimal Conditions for Live-Cell Imaging

Live-cell imaging is the investigation of dynamic physiological processes in living cells using time-lapse imaging from milliseconds to hours. Live-cell imaging turns multiple snapshots to movies, which is in contrast to fixed-cell imaging that examines cellular activity at a time point. Typical applications of live-cell imaging that are used to study kinetic events include enzyme…

Rose Spear: Engineering Talent IRead More
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