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Have I Got Good Protein Expression News for You

While the art of reading hand poured sequencing gels is forgotten as the next generation sequencers spit out genome after genome, protein expression still often requires sweat, tears and a lot of luck. I think this happens not because there is something different about protein expression in comparison with sequencing, but because people do not think about protein expression as an engineering challenge.

But where there is engineering, there should be a manual, right? Neither the literature reviews full of confusing technical details, nor protocols, which tell you how to do something but not why.

What you need is something in between, a simple and may be even funny introduction into protein expression, how to select parts and optimise production. The good news is that our site is launching The Manual – a eBook called “The Bitesize Bio Guide to Protein Expression”. Written by a team of hands-on molecular biologists: an experienced postdoc and prolific BsB writer (me) and edited by an excellent scientific editor Emily Crow.

Check out the e-book content:

Chapter 1 Introduction

Chapter 2 Choosing an expression system

What is an expression system anyway?

    Choosing your heterologous expression system

    Vectors

A horse called Escherichia

    There is more to E. coli than BL21: Strain choice

    Other parameters that you have at your disposal

Fifty shades of yeast

    The species

A very friendly armyworm

    Insect cell lines

    Vectors for expressing heterologous proteins in insect cells

Popular (cell) culture

    Cell lines

    Vectors

I want to be (cell) free

   System overview

   Why would you use cell extracts?

Chapter 3 Exogenous protein expression: the problems

Why you need to know basic facts about your protein

Coding 101: Codon optimization 26

Are you content with your gene content?

Repeat sequences: Lego blocks of secondary structure

The game of introns

The importance of being soluble

The art of biological origami, or: Consider folding

    Mess with protein sequence at your peril

    Not so fast: How speed of expression can screw up your protein folding

    But I couldn’t possibly fold without my chaperone!

    Why won’t my freaky protein fold?

Return to sender: Expressing a protein in the wrong compartment

Export your protein

What if the expressed protein is toxic?

Conclusion

Pick up your copy of the eBook “The Bitesize Bio Guide to Protein Expression” today.

And if you want to know more about protein expression watch this webinar where I discuss the ins and outs of protein expression.

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