Techniques / Protein Expression & Analysis

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Article

Titering Phage – Counting Something Invisible with Something Only Slightly More Visible

Titering Phage - The Plaque Assay Phage display is a molecular technique used to isolate binding or interaction partners to molecules of interest from an extensive library. Such libraries are often derived...
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Article

Proteomics and Good Mass Spectrometry Data

It is currently possible to analyze thousands of proteins in a single sample using mass spectrometry (MS) and a database of predicted protein sequences, referred to as ‘bottom-up’ proteomics. With this...
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Article

Benefits of the GUS Gene Reporter System in Plants

Gene reporters enable valuable insight into gene expression. The GUS gene reporter system is one of the popular and common plant reporter systems. GUS,  is short for glucuronidase, an enzyme in the bacterium...
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Article

Buffer Banter: Pre-cast PAGE Gels & Buffer Compatibility

This article is not for the die-hard old-school gel runners. You know who you are, the purists, the “I always make my own gels and buffers from scratch” kind. For you we have lots of articles about PAGE...
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Article

Extracting Better Ubiquitin Data from Your Samples: Beyond the Cellular Skip

The ubiquitin-proteasome system was discovered at the start of the 1980s, and people have been studying it ever since. Initially, researchers thought that tagging a protein with ubiquitin was the cell’s...
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Examining Cell Interactions with Surface Plasmon Resonance (SPR) and Identifying Epitopes using SPR-Mass Spectrometry (MS)

Surface plasmon resonance (SPR) offers highly efficient, label-free detection for quantifying biomolecular interactions in real-time. Two exciting SPR variants that have sprung up in recent years are SPR for...
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Article

All Charged Up: The Basics of Ion-Exchange Chromatography

Highly pure proteins are vital for successful experiments; they play roles in research as assay reagents (especially for SPR applications), therapeutic candidates, and of course, as the subjects of structural...
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Article

A New Frontier in Protein Quantitation: AlphaLISA

If you ever worked in a biology or biochemistry laboratory, you probably already heard about ELISA. You may have even used it. But do you know what’s behind it? And how you can improve it? Let me guide you...
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Article

How to Scrutinize Your Glycosylated Proteins Without Using Glycosidases

You might have come across protein glycosylation before. Somewhere in the recesses of your memory you might even recall reading something about the protein you’re studying being glycosylated, but what does...
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Key Analytical Challenges for Antibody Drug Conjugates

Currently, there are more than 75 antibody drug conjugates (ADCs) in various stages of pre-clinical and clinical development. The combination of a targeted antibody coupled with a cytotoxic small-molecule drug...
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