Established in the mid 1970's, New England Biolabs, Inc. (NEB) is the industry leader in the discovery and production of enzymes for molecular biology applications and now offers the largest selection of recombinant and native enzymes for genomic research. NEB continues to expand its product offerings into areas related to PCR, gene expression, sample preparation for next generation sequencing, synthetic biology, glycobiology, epigenetics and RNA analysis. Additionally, NEB is focused on strengthening alliances that enable new technologies to reach key market sectors, including molecular diagnostics development. New England Biolabs is a privately held company, headquartered in Ipswich, MA, and has extensive worldwide distribution through a network of exclusive distributors, agents and seven subsidiaries located in Canada, China, France, Germany, Japan, Singapore and the UK. For more information about New England Biolabs visit neb.
In 2007, I wrote a couple of articles on the best free software for molecular biologists on Mac and PC. One of the highlights of those lists was a fantastic cross-platform for in silico DNA cloning, sequence analysis and visualization, called Serial Cloner (move over VectorNTI!).
The creator of Serial Cloner, Franck Perez, contacted me recently to tell me that he had just released an excellent new version of the software and asked if I would relay this to our readers. Well, as a big fan of Serial Cloner I am only too happy to oblige.
If you do molecular biology and you haven’t tried our Serial Cloner, I’d urge you to give it a go. It is very intuitive and is packed with features; from basics like constructing importing sequences, constructing plasmid maps and restriction mapping, through more complex things like sequence alignment, Gateway cloning and siRNA design.
Among the many improvements Franck has made in this new version are:
- Automatic scanning for features in imported sequences
- Importing VectorNTI, Genbank, EMBL, ApE sequences with features
- Generation of MultiFasta formated sequence for Clustal submission
- Recovery of unsaved sequence in case of unexpected quit
- Impoved Import of NCBI/EMBL sequences (Web Access)
- Alignments via NCBI BLAST2seq
Check it out at http://serialbasics.free.fr/Serial_Cloner.html