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Why do you get three bands when running uncut plasmid DNA on agarose gels. Discover the answer and how it can help improve your DNA plasmid preps.
last updated: August 1, 2024
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We use fluorescent in situ hybridization (FISH) techniques routinely to detect DNA or RNA sequences in tissues, but what about micro RNAs (miRNAs)? No worries, FISH is now optimized to meet the challenge. To help you get going with the method, here’s what you need to know. The first thing that comes to mind when…
Red Pill or Blue? Carrying out science often involves many difficult decisions! I see it all the time in RNA protocols – the “gracious” option of using purified water or Tris-EDTA (TE) buffer to dissolve (or elute, if you are using column purification) RNA. When I was trained in assessing RNA using UV spectrophotometry, graduate…
The Phobia of RNases My first experience of troubleshooting in vitro transcription came when I was synthesizing RNA In-Situ Probes for the first time. A lab mate ominously warned me that I had just returned to lab after a bout of flu and that meant I’m a walking talking factory of RNases. I went ahead…
Gene reporters enable valuable insight into gene expression. The GUS gene reporter system is one of the popular and common plant reporter systems. GUS, is short for glucuronidase, an enzyme in the bacterium E. coli. GUS is a good reporter for plants, as it does not occur naturally, and thus, has a low background. With…
The Basic Local Alignment Search Tool (BLAST) algorithm is at the heart of a free suite of online resources available through the National Center for Biotechnology Information (NCBI). While most researchers are aware of BLAST as a sequence alignment tool, NCBI’s BLAST suite offers so much more! I’ll cover in-depth how to use these resources…
MicroRNAs (miRNA) are short, non-coding RNAs involved in post-transcriptional silencing of gene expression. miRNAs can be associated with exosomes and can function as cancer-specific biomarkers. This, coupled with the fact that they are stable in plasma and serum makes them valuable diagnostic tools, as long as they can be reliably isolated from the serum and…
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