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One Tube PCR Cloning Method
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I have to say I was pretty sceptical, but I had a go using one of my own vectors and got pretty good results back. The background was higher than normal, but I certainly got the clones. I think that although this won’t work in all cases, it is a great method for routine cloning… thanks Chun-Ming!
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hello Dr Oswald
thanks for the site it’s really helpfull!
i’d like to know if this protocol is convenient for blunt end cloning ( i use T4 DNA pol to blunt my vector and insert as well) and what about the buffers ?