Addgene is a non-profit plasmid repository that stores and distributes plasmids for academic labs. It’s great if you work in an academic lab and they happen to have your plasmid – drop them an order and get your plasmid in the mail. I don’t work in an academic lab, but I still love addgene… here’s why…
In addition to their repository they also have a great (and free) searchable plasmid archiving facility called addgene labs. Opening an account for your lab gives you a laboratory plasmid archives database and also a personal database for your own plasmids, which can be viewed by others in your lab. You can also send e-mail invites to lab members, allowing them to get their own database within your lab account.
As far as I am aware there are no similar services available on the web so I have just signed up… now I can say goodbye to Excel files full of plasmid lists!
The most comprehensive way to evaluate DNA concentration and purity is to use both UV spectrophotometeric measurements and agarose gel eletrophoresis. This quick reference guide gives an overview of the information that can be derived from both. UV spectrophotometric measurement of DNA concentration and purity DNA itself, and most of the common contaminants found in […]
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