Addgene is a non-profit plasmid repository that stores and distributes plasmids for academic labs. It’s great if you work in an academic lab and they happen to have your plasmid – drop them an order and get your plasmid in the mail. I don’t work in an academic lab, but I still love addgene… here’s why…
In addition to their repository they also have a great (and free) searchable plasmid archiving facility called addgene labs. Opening an account for your lab gives you a laboratory plasmid archives database and also a personal database for your own plasmids, which can be viewed by others in your lab. You can also send e-mail invites to lab members, allowing them to get their own database within your lab account.
As far as I am aware there are no similar services available on the web so I have just signed up… now I can say goodbye to Excel files full of plasmid lists!
One of the most common tasks in molecular biology is cleaning up DNA from aqueous solutions to remove buffer salts, enzymes or other substances that could affect downstream applications. Examples include cleaning up PCR reactions, digests or other enzymatic treatments and cleaning up genomic or plasmid DNA contaminated with cellular proteins/debris. There are several ways […]
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