Techniques / DNA / RNA Manipulation and Analysis

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Choosing the Right E. coli Strain for Transformation

Cloning, purifying, and expressing modified genetic material is routinely done in microbes such as Escherichia coli (E.coli). Relatives of this molecular biology workhorse normally live in the intestinal track...
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Article

SAGE Part 2: LongSAGE, RL-SAGE and SuperSAGE

SAGE, or serial analysis of gene expression, is a technique that enables you to digitally analyze the entire gene expression profile of a cell(s). Before this technique, scientists were limited to studying a...
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Article

Vital for Soup, Vital for Labs: Serial Analysis of Gene Expression (SAGE), part 1

Some techniques can sound very dry but this isn't one of them! SAGE was first described and published by Velculescu et al. in 1995. At the time, techniques like RNA blotting and expressed sequence tagging...
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How to Choose Your Method for DNA Extraction from Whole Blood

The technique you choose for whole blood DNA extraction impacts not only your results but also the ease of your workflow. It can be difficult to choose which method to use. Almost all of the available protocols...
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Eight Top Tips to Maximize Yield from Whole Blood DNA Isolation

When you perform genomic DNA extraction from whole blood, low yield or low quality DNA can result in many issues. No matter your intended downstream application—qPCR, next generation sequencing, Sanger...
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Article

Essential Factors for Successful Gateway Cloning

An efficient cloning system that is in vogue at present is the Gateway® Cloning Technology. Gateway cloning gives researchers the opportunity to easily transfer DNA fragments into plasmids using proprietary...
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Article

Demystifying REBASE and NEBcutter

Two years ago, all I knew was third BASE in my baseball field and the cutter ball from the pitcher. Now, I know a lot more about lab-based BASES and cutters: REBASE and NEBcutter. While they sound like baseball...
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Article

How to Make Your Own Chemically-Competent Cells

I once had the terrible experience of not being able to run an assay because I ran out of commercial stock of transformation-competent Escherichia coli (E.coli). From that day, I learned to make my own...
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Article

How to Improve Plasmid Yield Using Antibiotics

After the initial excitement of growing and isolating plasmid DNA, routinely preparing plasmid mini/midi/maxi preps gets boring. You definitely want a way to squeeze the maximum amount of plasmid DNA out of...
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Article

A Quick Guide to Screening by Restriction Digestion

PCR screening is great for identifying potential positive clones after cloning/subcloning. However, you’ll still want to confirm that the DNA sequence is correct for any positives identified. The easiest and...
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