Techniques / DNA / RNA Manipulation and Analysis

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Extract HMW DNA for Long-Range Genomic Analysis with the New SageHLS Platform

Long-Range Genomic Analysis After a decade of DNA genomic sequencing being restricted to short reads, new technologies are making it possible for scientists to capture long-range genetic information that is...
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Four Ways to Get CRISPR Reagents Into Your Cells

Do you need to test the effects of mutating a gene in your system?  Then, CRISPR is the way to go. Your first step is to decide on good target sequences. Then, you have to get the two components of CRISPR: the...
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Article

Cloning Large or Complex DNA Fragments

Sometimes you know a project is going to be a pain before you even start it. For me, that is whenever I need to clone large (> 3 kb) or complex (e.g., a sequence with repeats) DNA fragments. Long...
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How DNA Extraction Is Different in Plants

I know what you are thinking, everything is made of cells, so how different can DNA extractions be in plants? The answer is… sort-of different. The overall concept is the same. Cell membranes are lysed, DNA...
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CRISPR Technology Explained: Towards a CRISPR Genome!

Okay, if you are working in the biomedical research field and you have not heard about CRISPR technology, then you are way behind the trend curve. Don’t worry; I would like to share what I have learned about...
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Article

The Three Ts of Introducing Foreign DNA: Transfection, Transduction, and Transformation

Introducing foreign DNA into bacterial or eukaryotic cells is a common molecular biology technique. Some of the terminology involved can be confusing: transformation, transfection and transduction. What do...
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Article

Viral Vector Production: Myths & Misconceptions

Viral vector production is a worthwhile skill that can be made even easier with a few tips and tricks. In general, transfection of multiple plasmids into a producer cell line results in infectious,...
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AAV Production Part II: Virus Purification

In Part I of AAV Production, I described how to produce crude (non-purified) AAV. In this article, I am going to tell you how to purify that crude prep. Virus purification is usually done by gradient...
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Article

How to Dephsophorylate DNA Using Calf Intestinal Phosphatase

When you dephosphorylate a vector for cloning, you remove the 5' phosphate groups required by DNA ligase to join the phosphodiester DNA backbone together. This prevents your vector from ligating back to itself...
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Article

Acid Phenol Chloroform Extraction of DNA, RNA and protein: 3 in 1

In austerity times, nothing is in excess. Apart from saving reagents, which can be refilled with extra financial injections, there is a commodity that cannot be easily resupplied – tissue samples! If, like...
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