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DNA / RNA Manipulation and Analysis

The Beginner’s Guide to Reading Plasmid Maps

Plasmid maps are a cornerstone of biology, but they are confusing to read for beginners. Our easy guide tells you how to read them, where to look for essential information, and how to avoid common mistakes.

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Plasmid vs. Genomic DNA Extraction: The Difference

Most experiments start with a piece of DNA—either plasmid DNA or genomic DNA. And your downstream uses for it dictate how much you need, what contaminants you can tolerate, and your extraction and purification methods. In this article, we explain the key differences between plasmid and genomic DNA extraction methods.

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Multiple Fragment Ligation: The Why and How

You may be familiar with standard single fragment ligations, but did you know you can ligate multiple fragments into your vector all at the same time! Discover how to perform multiple fragment ligation, including the different methods and troubleshooting tips for when things go wrong.

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Which Grade of Ethanol Should I Use?

Want to know more about ethanol grades commonly used in the lab? We help you make sense of your flammables cabinet with our rundown of the ethanol grades typically used in molecular biology, as well as some important rules for how to use them correctly.

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What’s The Problem With Ampicillin Selection?

Resistance to the antibiotic ampicillin is commonly used as a selection marker for plasmids in gene cloning and protein expression in E.coli and other bacteria. While it can be incredibly useful tool, there can be problems using this selection marker that you need to be aware of if if you plan on using it. This…

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