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Genomics and Epigenetics

How CRISPR Can Be Used to Detect Emerging Viral Pathogens

The threat of pandemic viruses can be mitigated by developing diagnostic tests that are rapidly implementable and field deployable. Two CRISPR-based strategies, DETECTR and SHERLOCK, have been developed recently that not only meet these criteria but offer unprecedented multiplexing capabilities. This article discusses the theoretical basis of proposed and currently available viral diagnostics for educational…

A detective dressed like a detective to symbolize the DETECTR and SHERLOCK CRISPR methods for Viral diagnosis.Read More

Plant vs. Virus: Comparative transcriptomic analysis of single TuMV-infected micro-dissected plant cells

In this webinar, you will learn: How studying the early events of virus infection in plants avoids the dilution effect and allows identification of the key molecular players involved in initial plant-virus interactions.  How the MMI CellCut microdissection system allows dissection of the first virus-infected plant cells during virus-plant interactions. The results of a comparative…

Arabidopsis thaliana in test tubes to depict study of viral infection of plants.Read More

How to Validate a CRISPR Experiment  

Discover how to validate the success of your CRISPR gene editing experiment from determining successful delivery of CRISPR reagents into your cells to the confirmation of desired genetic and phenotype changes.

Image of sequencing data to depict validation of successful CRISPR gene editing.Read More

Engineering Vero Cell Lines Using CRISPR to Increase Production of Viral Vaccines

Engineering Vero Cell Lines Using CRISPR to Increase Production of Viral Vaccines On Demand Webinar Dr. Benjamin Borgo Dr. Benjamin Borgo currently leads a team of forward-thinking product and service managers within Merck KGaA’s Genome Engineering and Modulation franchise. His first exposure to CRISPR-based genome editing was as a graduate student where he attempted to…

Production of Viral Vaccine can be enhanced using CRISPR genome-editingRead More

WGS Workflow: From Sample Collection to Data Interpretation

The efficiency of whole genome sequencing (WGS) workflows has skyrocketed since its inception. Major leaps and minor tweaks in the WGS workflow have compounded over time resulting in radical reductions in processing time and the cost of sequencing whole genomes over the past decades. The complete sequencing of the first human genome, named the Human…

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The Recipe for Successful Whole Genome Sequencing

The success of whole genome sequencing (WGS) is shown in the quick and efficient scientific response to the 2011 outbreak of E. coli in Germany and France.1 German and French strains of E. coli were indistinguishable using standard tests.  However, WGS analysis showed 2 single nucleotide polymorphisms (SNPs) in the German strains and 9 SNPs…

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CRISPR Nucleases: The Ultimate Guide To Selection

CRISPR nucleases continue to dominate the gene-editing landscape because they are powerful and easy-to-use gene-editing tools. Their rapid discovery and development, however, make choosing the right nuclease for your experiment difficult. This guide highlights the broad range of available CRISPR nucleases and distils the major factors you should consider when choosing one for your next…

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A Colorful Route to Sequencing Success

An oft-repeated maxim in biological bench science is that any experiment is only as good as its control. A control is an unchanging standard of comparison in an experiment, and an internal control is typically a standard reaction run together with the test reaction in the same reaction mixture. The purpose of an internal control…

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CRISPR Multiplexing Strategies: What’s The Choice?

Although multiplex CRISPR gene editing can be accomplished by simply introducing more than one gRNA to your target cells, there are many alternative — and more efficient — ways of achieving this goal. This article discusses these alternative CRISPR multiplexing strategies and highlights their potential caveats. Not sure whether multiplex CRISPR gene editing is right…

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Multiplex CRISPR Gene Editing: What’s It For?

The RNA-guided nature of CRISPR nucleases (e.g., Cas9, Cpf1) makes them highly amenable to multiplex applications, including multi-gene knockout and large-scale genomic modifications. This article — part 1 of a two-part series on multiplex CRISPR gene editing — discusses these applications and outlines practical considerations for multiplex CRISPR gene editing experiments. New to CRISPR gene…

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Dead Useful: CRISPR-Cas9 Epigenome Editing

Given the rapid pace with which genome editing technologies have been developed and adopted, it comes as no surprise that the original CRISPR-Cas9 system has been successfully modified by some very clever scientists. No longer are we limited to the ‘simple’ case of editing the genetic sequence of your biological system of choice, but there…

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DNA Extraction for Next Gen Sequencing

The advent of Next Gen Sequencing (NGS) has been truly amazing. One of the marvels that is often overlooked is how advances in DNA extraction technology have helped streamline NGS workflows. The original standard – phenol/chloroform extraction – is not well suited to the automated nature of today’s sequencing workflows (though with the emergence of…

DNA size selection for NGS librariesRead More

Get Prepped: Nanopore Library Preparation Optimization

Nanopore is a relatively new sequencing platform and researchers are still trying to optimize the protocol for their own specific applications. In our lab, we work primarily with metagenomic samples and use the 1D sequencing kits. Over the past year, we have optimized this technique. To check the quality of the Nanopore library preparation we…

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