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Genomics and Epigenetics

WGS Workflow: From Sample Collection to Data Interpretation

The efficiency of whole genome sequencing (WGS) workflows has skyrocketed since its inception. Major leaps and minor tweaks in the WGS workflow have compounded over time resulting in radical reductions in processing time and the cost of sequencing whole genomes over the past decades. The complete sequencing of the first human genome, named the Human…

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The Recipe for Successful Whole Genome Sequencing

The success of whole genome sequencing (WGS) is shown in the quick and efficient scientific response to the 2011 outbreak of E. coli in Germany and France.1 German and French strains of E. coli were indistinguishable using standard tests.  However, WGS analysis showed 2 single nucleotide polymorphisms (SNPs) in the German strains and 9 SNPs…

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CRISPR Nucleases: The Ultimate Guide To Selection

CRISPR nucleases continue to dominate the gene-editing landscape because they are powerful and easy-to-use gene-editing tools. Their rapid discovery and development, however, make choosing the right nuclease for your experiment difficult. This guide highlights the broad range of available CRISPR nucleases and distils the major factors you should consider when choosing one for your next…

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A Colorful Route to Sequencing Success

An oft-repeated maxim in biological bench science is that any experiment is only as good as its control. A control is an unchanging standard of comparison in an experiment, and an internal control is typically a standard reaction run together with the test reaction in the same reaction mixture. The purpose of an internal control…

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CRISPR Multiplexing Strategies: What’s The Choice?

Although multiplex CRISPR gene editing can be accomplished by simply introducing more than one gRNA to your target cells, there are many alternative — and more efficient — ways of achieving this goal. This article discusses these alternative CRISPR multiplexing strategies and highlights their potential caveats. Not sure whether multiplex CRISPR gene editing is right…

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Multiplex CRISPR Gene Editing: What’s It For?

The RNA-guided nature of CRISPR nucleases (e.g., Cas9, Cpf1) makes them highly amenable to multiplex applications, including multi-gene knockout and large-scale genomic modifications. This article — part 1 of a two-part series on multiplex CRISPR gene editing — discusses these applications and outlines practical considerations for multiplex CRISPR gene editing experiments. New to CRISPR gene…

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Dead Useful: CRISPR-Cas9 Epigenome Editing

Given the rapid pace with which genome editing technologies have been developed and adopted, it comes as no surprise that the original CRISPR-Cas9 system has been successfully modified by some very clever scientists. No longer are we limited to the ‘simple’ case of editing the genetic sequence of your biological system of choice, but there…

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DNA Extraction for Next Gen Sequencing

The advent of Next Gen Sequencing (NGS) has been truly amazing. One of the marvels that is often overlooked is how advances in DNA extraction technology have helped streamline NGS workflows. The original standard – phenol/chloroform extraction – is not well suited to the automated nature of today’s sequencing workflows (though with the emergence of…

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