Search results for: CRISPR
Level Up Your Drug Screening With CRISPR
…human kinase pool, and custom CRISPR panels are available. Single-cell analysis is also possible with 10x Genomics compatible CRISPR pools. CRISPR Drug Screening Summarized CRISPR screening uses the CRISPR-Cas9 system…
Read MoreCRISPR Gene Editing: Considerations and Getting Started
…basic concepts and considerations of conducting a CRISPR experiment. We encourage you to explore the additional educational resources listed below. More CRISPR Resources CRISPR Articles How to Validate a CRISPR…
Read MoreWhy You Should Consider Adding CRISPRa and CRISPRi to Your Toolbox
…high-dimensional characterization of CRISPR perturbations. More CRISPR Resources CRISPR Articles CRISPR Gene Editing: Considerations and Getting Started How to Validate a CRISPR Experiment How to Understand CRISPR Formats and Their…
Read MoreA Brief History of CRISPR-Cas9 Genome-Editing Tools
…Adaptive Immune Response Works When a virus infects a prokaryote, the spacer sequences in CRISPR arrays are transcribed to generate short CRISPR RNA (crRNA), which guides the CRISPR-associated sequence (Cas)…
Read MoreHow to Understand CRISPR Formats and Their Applications
…CRISPR Lentiviral Formats CRISPR Controls CRISPR activation Cas9 Protein Comparison Sigma-Aldrich® Advanced Genomics Additional CRISPR Webinars Discover more about CRISPR in the Bitesize Bio CRISPR Research Hub. References Chatterjee, P….
Read MoreCRISPR Technology Explained: Towards a CRISPR Genome!
…enhance the specificity of the knockout region.3 CRISPR Technology in Action Scientists are already testing the power of CRISPR technology, as witnessed by the surge in CRISPR technology-related publications. Some of…
Read MoreA CRISPR Approach to Immuno-Oncology
…CRISPR Articles CRISPR Gene-editing: Considerations and Getting Started How to Validate a CRISPR Experiment How to Understand CRISPR Formats and Their Applications CRISPR Webinars Engineering Vero Cell Lines Using CRISPR…
Read MoreCRISPR Nucleases: The Ultimate Guide To Selection
…CRISPR-related plasmids and viral preparations. How to Choose a CRISPR Nuclease As mentioned above, size and PAM specificity are critical parameters that you should focus on when choosing a CRISPR…
Read MoreHow to Validate a CRISPR Experiment
…CRISPR Gene Editing 101 CRISPR Tools And you should also consider the following Sigma-Aldrich® resources: CRISPR Lentiviral Formats CRISPR Controls CRISPR Essentials Quick ordering Advanced Genomics Resource Center Sigma-Aldrich® Advanced…
Read MoreMultiplex CRISPR Gene Editing: What You Need to Know
…practical considerations for multiplex CRISPR gene editing experiments. We also discuss the various multiplex CRISPR strategies available and highlight their potential caveats. New to gene editing with CRISPR? Get a…
Read MoreHow to Design a CRISPR Cas9 Experiment and Start Genome Editing
CRISPR and the CRISPR Associated system (Cas) are powerful gene-editing technologies. Originally identified and characterized in bacteria, the endogenous CRISPR systems act as an RNA-based defense mechanism against invading phage…
Read MoreCRISPR Genome Editing: What You Need to Know to Get Started
…sure if CRISPR is right for you, you should weigh up the pros and cons of CRISPR first. Although in some cases, CRISPR Cas9 gene editing can be used on whole…
Read MorePros and Cons of CRISPR: Simple Tips for Weighing Up CRISPR for Genome Editing
…Design a CRISPR Experiment and Start Genome Editing How to Confirm Your CRISPR-Cas9 Genome Editing Was Successful Optimizing a CRISPR-Cas9 protocol can be challenging and time-consuming. But with skill, luck,…
Read MoreA Better Alternative to Immortalized Cells: Combining CRISPR and iPSCs
…on CRISPR. A word to the wise—when designing guides for your CRISPR experiments, avoid ones that introduce mutations into the PAM sequence. Practical Guidance for CRISPR iPSCs Genome Editing Projects…
Read MoreGet Started in Genome Editing with CRISPR
The development of CRISPR/Cas9 technology has made it relatively straightforward to selectively edit genomes and has revolutionized the way in which we approach biological questions. CRISPR stands for Clustered Regularly…
Read MoreHow CRISPR Can Be Used to Detect Emerging Viral Pathogens
…more about CRISPR? Check out our ultimate guide to CRISPR nucleases and our guides on multiplexing using CRISPR (part 1 and part 2). Discover more about CRISPR in the Bitesize…
Read MoreFour Ways to Get CRISPR Reagents Into Your Cells
…are many ways to deliver CRISPR reagents into cells, and it can be hard to know which one to choose. This article focuses on delivery into cells, but CRISPR can…
Read MoreCRISPR Multiplexing Strategies: What’s The Choice?
…1 of this two-part guide: Multiplex CRISPR Gene Editing: What’s It For?. Two Main CRISPR Multiplexing Strategies Many CRISPR multiplexing strategies have been developed to improve CRISPR applications [1]. These…
Read MoreControlling Gene Expression with CRISPR Interference (CRISPRi)
…system you can either activate or repress gene expression. From CRISPR to CRISPR Interference The usual CRISPR system is composed of 2 components, a “guide” RNA (gRNA) and a CRISPR-associated…
Read MoreA Crash Course in CRISPR-Cas9 Editing in Drosophila
…like to share with you an efficient strategy, originally developed by Port et al.,3 that we adopted to successfully make three different CRISPR-Cas9 knock-in fruit flies. Challenges in Performing CRISPR…
Read MoreDead Useful: CRISPR-Cas9 Epigenome Editing
…dCas9 constructs can be found at the Addgene CRISPR Plasmids and Resources website. Good luck! Discover more about CRISPR in the Bitesize Bio CRISPR Research Hub. References Vojta et al….
Read MoreCRISPR-Cas13: How to Revolutionize Your RNA Research
…CRISPR effector. Science 353(6299): aaf5573. Granados-Riveron J et al. (2018) CRISPR-Cas13 Precision transcriptome engineering in cancer. Cancer Res. 78(15):4107–1413 Ai Y et al. (2022) CRISPR/Cas13 effectors have differing extents of…
Read MoreHow to Confirm Your CRISPR-cas9 Genome Editing Was Successful
…biological processes. But more than that, it proves to the world that you are a talented and able scientist. Discover more about CRISPR in the Bitesize Bio CRISPR Research Hub….
Read MoreCRISPR-Based Activation (CRISPRa): A How-To Guide
…CRISPRa experiments. For more depth, see the following technical resources: The Weissman Lab webpage on CRISPR Broad Institute Protocols Discover more about CRISPR in the Bitesize Bio CRISPR Research Hub….
Read MoreHow to Find Your Mutated Cells After Using CRISPR-Cas Genome Editing
Once you’ve carried out CRISPR-Cas (or other approaches) to generate mutations in your cells of interest, the next step is identifying CRISPR edited cells. Then, you can generate clonal populations…
Read MoreA Step-by-step Guide to Designing Cas13 gRNAs
…RNA targeting with CRISPR-Cas13. Nature. 550(7675);280–284. Cox, D. et al. (2017) RNA editing with CRISPR-Cas13. Science, 358;1019–1027 Konermann, S, et al. (2018) Transcriptome Engineering with RNA-Targeting Type VI-D CRISPR Effectors….
Read MoreCRISPR-Inspired Method Targets Large, Repetitive DNA Elements
…need. Fortunately, the development of the CRISPR/Cas9 system has dramatically changed the landscape of genome editing. Advances with this system have inspired new methods for targeting a specific genomic region….
Read MoreShow Your Molecular TALEN(T)
…will be revealed. If you are interested in finding out the differences between TALEN and CRISPR are, feel free to check out the following articles at Bitesize Bio: CRISPR Technology…
Read MoreTop 10 Uses of Microbes in Biotechnology
…used to introduce stable changes into the genetic material of eukaryotic cells as well. Where to use CRISPR. How to start with CRISPR. How to validate CRISPR experiment. And particular…
Read MoreWhy You Should Use Cas9 Ribonucleoprotein Transformation for CRISPR Genome Editing
Imagine directly creating a mutation at (almost) any site in your target genome instead of screening thousands or millions of random mutants! The CRISPR/Cas9 system does just that. In its…
Read MoreAntibody Validation
…Genetic Strategies: CRISPR-Cas9 and More Genetic strategies consist of techniques like CRISPR-Cas9/KO, RNAi, and siRNA knockdown, used in conjunction with a protein detection assay. These methods detect any non-specific binding…
Read MoreGeneDig: An Easy-to-use Genome Browser for Bioscientists
The recent advancement of next generation sequencing technology and the development of novel gene editing tools, such as CRISPR-Cas9, have revolutionized research in genetics. In this golden era of molecular…
Read MoreHow to Test the Efficiency of your sgRNA
…zebrafish, SSC, Sequence Scan for CRISPR, or WU-CRISPR that you can use to predict the efficiency and the suitability of the sgRNA. However, the prediction efficiency of these computational tools…
Read MoreMicroglial Cell Lines: The Ultimate Guide to Selection
…It was used recently in CRISPR/Cas9 screening assays to identify genetic regulators of impaired phagocytosis and lipid droplet formation. [5, 6] These screens provided key insights into microglial biology that…
Read MoreMini Me: What Makes for Good Models of Human Disease?
…human diseases with a clear genetic cause. For example, mice with Duchenne Muscular Dystrophy have cured using the revolutionary gene-editing CRISPR/Cas9 technology9. Take Home Message No single animal model recapitulates…
Read MoreFive Ways to Modify Your siRNA for Improved RNAi
Despite the rising popularity of CRISPR/Cas genome engineering, RNA interference (RNAi) still remains a widely used tool in the life scientist’s toolbox (brush up on the mechanism of RNAi here)….
Read MoreOutsourcing Research: Should Your Experiment Spend Some Time Away from You?
…sequencing, CRISPR, gene synthesis and many other services are now commercially available. It’s even possible to buy transgenic and CRISPR mice essentially off-the-shelf. But how do you decide whether outsourcing…
Read MoreThe art of generating single cell clones
Making mutations in mammalian cell lines is becoming much easier, especially with advanced molecular engineering techniques such as CRISPR/Cas9, among others. However, after making a mutation, do you know if…
Read MoreSimple Tips for Model Organism-Based Work
…case of unexpected delays. Recent advances in CRISPR-based mouse gene targeting techniques have undoubtedly opened new doors for scientific advancement in health, but they have also made the task of…
Read MoreCre-loxP Recombination Essentials Part 2
…the advent of CRISPR-Cas9 technology, genetic modification using the Cre-loxP system may one day become obsolete. However, until every lab can afford to CRISPR their way through target genes, we…
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