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Multiplex CRISPR Gene Editing: What’s It For?

…two-part series on multiplex CRISPR gene editing — discusses these applications and outlines practical considerations for multiplex CRISPR gene editing experiments. New to CRISPR gene editing? A general overview can…

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CRISPR Multiplexing Strategies: What’s The Choice?

…1 of this two-part guide: Multiplex CRISPR Gene Editing: What’s It For?. Two Main CRISPR Multiplexing Strategies Many CRISPR multiplexing strategies have been developed to improve CRISPR gene editing applications…

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Controlling Gene Expression with CRISPR Interference (CRISPRi)

…al. (2013) Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression. Cell. 152(5): 1173-83. CRISPR/Cas9 Guide: Web site https://www.addgene.org/crispr/guide/ Qi and Weissman Labs CRISPR plasmids: Website https://www.addgene.org/crispr/qi-weissman/…

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Dead Useful: CRISPR-Cas9 Epigenome Editing

…be found at the Addgene CRISPR Plasmids and Resources website. Good luck! References Vojta et al. (2016) Repurposing the CRISPR-Cas9 system for targeted DNA methylation. Nucleic Acids Res. 8;44(12):5615-28. doi:…

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Show Your Molecular TALEN(T)

…Explained: Towards a CRISPR genome! Getting Started in Genome Editing with CRISPR CRISPR Genome-Editing – What You Need to Know to Get Started References Wright et al. TALEN-mediated genome editing:…

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How to Test the Efficiency of your sgRNA

…zebrafish, SSC, Sequence Scan for CRISPR, or WU-CRISPR that you can use to predict the efficiency and the suitability of the sgRNA. However, the prediction efficiency of these computational tools…

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Mini Me: What Makes for Good Models of Human Disease?

…human diseases with a clear genetic cause. For example, mice with Duchenne Muscular Dystrophy have cured using the revolutionary gene-editing CRISPR/Cas9 technology9. Take Home Message No single animal model recapitulates…

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The art of generating single cell clones

Making mutations in mammalian cell lines is becoming much easier, especially with advanced molecular engineering techniques such as CRISPR/Cas9, among others. However, after making a mutation, do you know if…

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