Search results for: CRISPR
CRISPR Technology Explained: Towards a CRISPR Genome!
…system. So far, three different CRISPR pathways have been mapped out, and the CRISPR II system seems to be the most prominent (Figure 1). The CRISPR system establishes immunity by…
Read More Multiplex CRISPR Gene Editing: What’s It For?
…two-part series on multiplex CRISPR gene editing — discusses these applications and outlines practical considerations for multiplex CRISPR gene editing experiments. New to CRISPR gene editing? A general overview can…
Read More How to Design a CRISPR Experiment and Start Genome Editing
CRISPR and the CRISPR Associated system (Cas) are powerful gene editing technologies. Originally identified and characterized in bacteria, the endogenous CRISPR systems act as an RNA-based defense mechanism against invading…
Read More CRISPR Multiplexing Strategies: What’s The Choice?
…1 of this two-part guide: Multiplex CRISPR Gene Editing: What’s It For?. Two Main CRISPR Multiplexing Strategies Many CRISPR multiplexing strategies have been developed to improve CRISPR gene editing applications…
Read More CRISPR-Cas9 Genome Editing: Weighing the Pros and Cons
…Genome Editing – What You Need to Know to Get Started Get Started in Genome Editing with CRISPR How to Design a CRISPR Experiment and Start Genome Editing How to…
Read More CRISPR Genome Editing – What You Need to Know to Get Started
Most people in the world of biology have heard of CRISPR-Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats-CRISPR associated systems) genome editing by now. This article aims to give you some…
Read More Controlling Gene Expression with CRISPR Interference (CRISPRi)
…al. (2013) Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression. Cell. 152(5): 1173-83. CRISPR/Cas9 Guide: Web site https://www.addgene.org/crispr/guide/ Qi and Weissman Labs CRISPR plasmids: Website https://www.addgene.org/crispr/qi-weissman/…
Read More Dead Useful: CRISPR-Cas9 Epigenome Editing
…be found at the Addgene CRISPR Plasmids and Resources website. Good luck! References Vojta et al. (2016) Repurposing the CRISPR-Cas9 system for targeted DNA methylation. Nucleic Acids Res. 8;44(12):5615-28. doi:…
Read More A Crash Course in CRISPR-Cas9 Editing in Drosophila
…Should You Use? The transgenic CRISPR/Cas9 strategy is efficient and consistent. However, the improved CRISPR activity may result in increased mortality. If the genes that you want to edit or…
Read More How to Find Your Mutated Cells After Using CRISPR-Cas Genome Editing
Once you’ve carried out CRISPR-Cas (or other approaches) to generate mutations in your cells of interest, the next step is to identify those cells that have been successfully edited. Then,…
Read More CRISPR-Based Activation (CRISPRa) of Genes: A How-To Guide
The purpose of this article is to walk you through the steps needed to overexpress genes using CRISPR/Cas9-based activation (CRISPRa). A broader overview of this topic (including CRISPR-based repression) can…
Read More CRISPR-Inspired Method Targets Large, Repetitive DNA Elements
…need. Fortunately, the development of the CRISPR/Cas9 system has dramatically changed the landscape of genome editing. Advances with this system have inspired new methods for targeting a specific genomic region….
Read More Show Your Molecular TALEN(T)
…Explained: Towards a CRISPR genome! Getting Started in Genome Editing with CRISPR CRISPR Genome-Editing – What You Need to Know to Get Started References Wright et al. TALEN-mediated genome editing:…
Read More Why You Should Use Cas9 Ribonucleoprotein Transformation for CRISPR Genome Editing
Imagine directly creating a mutation at (almost) any site in your target genome instead of screening thousands or millions of random mutants! The CRISPR/Cas9 system does just that. In its…
Read More How to Confirm Your CRISPR-cas9 Genome Editing Was Successful
…a note of the size of the amplicon and the size of your expected deletion. Extract DNA from both your CRISPR-Cas9 genome edited cell population and your unedited control population….
Read More How to Test the Efficiency of your sgRNA
…zebrafish, SSC, Sequence Scan for CRISPR, or WU-CRISPR that you can use to predict the efficiency and the suitability of the sgRNA. However, the prediction efficiency of these computational tools…
Read MoreGeneDig: An Easy-to-use Genome Browser for Bioscientists
The recent advancement of next generation sequencing technology and the development of novel gene editing tools, such as CRISPR-Cas9, have revolutionized research in genetics. In this golden era of molecular…
Read More Mini Me: What Makes for Good Models of Human Disease?
…human diseases with a clear genetic cause. For example, mice with Duchenne Muscular Dystrophy have cured using the revolutionary gene-editing CRISPR/Cas9 technology9. Take Home Message No single animal model recapitulates…
Read More Outsourcing Research: Should Your Experiment Spend Some Time Away from You?
…sequencing, CRISPR, gene synthesis and many other services are now commercially available. It’s even possible to buy transgenic and CRISPR mice essentially off-the-shelf. But how do you decide whether outsourcing…
Read More The art of generating single cell clones
Making mutations in mammalian cell lines is becoming much easier, especially with advanced molecular engineering techniques such as CRISPR/Cas9, among others. However, after making a mutation, do you know if…
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