Techniques / Microscopy & Imaging

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Revealing Cellular Dynamics with Millisecond Precision – The New Tool That Turned Electron Micrographs into Motion Pictures of Neural Communication

What if you can dissect the cellular dynamics with millisecond precision? What if you can unravel the morphological transformation of a neuron millisecond by millisecond using electron microscopy? Could this be even possible? In this webinar, we will talk about how optogenetics coupled with high-pressure freezing can make this possible. We will discuss how to […]

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In Microscopy & Imaging 29th of March, 2017 Presented by: Frédéric Leroux, Shigeki Watanabe
Article

In Situ Zymography: Let’s Catch that Enzyme in Action!

In situ zymography (ISZ) is the best choice to study proteases. Proteases are a challenge to study as proteases are extremely potent enzymes. As such, they need to be controlled at multiple levels to prevent...
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Article

Automated Cell Counting with a Fluorescent Twist

Cell counting is the bane of existence of many researchers. Countless hours spent in front of the microscope with a haemocytometer on the stand and a manual tally (or “clicker”) in hand can be really...
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Article

Analyze Immunostained Slides with Semiquantitative Scoring

A  routine task in the lab is to investigate the presence of your favorite protein in a range of histological samples. No doubt, staining your tissue sections using good old immunohistochemistry (IHC) would be...
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Article

Tips for Peering into the Interior of Mice Using Intravital Microscopy

Intravital microscopy (IVM) is a newer approach for the imaging of living tissues and organs in live animals. A wide variety of organs can be imaged in rodents–eyes, kidneys, brain, lymph nodes, and gut, to...
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Data Analysis for Three-dimensional Volume Scanning Electron Microscopy

In recent years, three-dimensional (3D) scanning electron microscopy techniques have gained recognition in the biological sciences. In particular, array tomography, serial block face scanning electron...
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Article

Can You Stand the Cold? Cryosectioning for Beginners

Before you can perform histology on your tissue samples – you need to prep them. This means you must fix them, embed them and section them into thin slices for analysis. A great way to slice your tissues is...
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Article

Microscopy – a Numbers Game

While the microscope is synonymous with biology, it is a child of physics and technology. When we learn about the microscope we learn physics—specifically, we learn about optics. Many great resources are...
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Article

The Art and Science of Figure Creation: Think BIG to see Small

There are those of us who began our careers literally in the dark. Yes, there was a time and not that long ago, that all figures had to be on film. Slide presentations were slides. Micrographs were, well,...
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Article

Halogen vs LED Lighting in Digital Microscopy

When it comes to light sources for microscopy, there is really no such thing as the “best.” The type of light source you use depends on the system you are working with and the type of result that you want....
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Challenges of Autofluorescence in Neuroscience

If you have ever imaged biological samples, you have likely encountered autofluorescence. That pesky background coloration you see under the microscope, which can make it difficult to distinguish your actual...
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