Search results for: library prep
Quantifying Your NGS Libraries
…very high-throughput. Illumina recommends the very capable KAPA library quantification kit, but there are alternatives including Agilent, Qiagen and QuantBio, among others. The KAPA kit is a SYBR-green assay that…
Read More The Cell: An Image Library-CCDB – Tutorial Part 2
This is the second in a continuing series of tutorials on getting the most from The Cell: An Image Library-CCDB. The first tutorial described setting up and using a free…
Read MoreHow to Maximize the Quantity and Quality of Purified Plasmids
Before spin columns, PCR, and reaction miniaturization, being able to maximize plasmid prep DNA yield was a highly competitive lab accomplishment. A card-carrying Molecular Biologist had to be able to…
Read More 5 Ways to Really Screw Up Your RNA Prep
…target and degrade RNA. In this article, I will discuss five mistakes that will no doubt hype your paranoia about the possible ways to screw up your RNA prep. 1….
Read MoreAn Introduction to RNA-seq
…library prep which includes end-repair, adapter ligation and PCR amplification to give you a library ready for sequencing. Why Bother With Strand Information? There has been a lot of discussion…
Read More Generating RNA-seq Libraries from RNA
…starts with size selection for small RNAs, ligation of selected fragments to adapters, and then generation of cDNA.4 New RNA Library Preparation Method A new RNA library prep method creates…
Read More Solid Phase Reversible Immobilization: How To Get A Bead On The Clean-up Of Your NGS Libraries
…concerned with reproducible library preps, gel purification after adaptor ligation is the step that requires the most attention to detail (see James Hadfield’s excellent instructions of what you need to…
Read MoreAAV Production Part II: Virus Purification
Sigma-Aldrich sells iodixanol as ‘Optiprep’. See the paper ‘AAV-mediated gene targeting methods for human cells’ for the different iodixanol solution recipes. First, prepare PBS-MK and NaCl/PBS-MK buffers. PBS-MK buffer: Dissolve…
Read More A Menagerie of Mini Prep Methods
Over the years there have been a multitude of methods – ranging from the easy to the downright painful – developed for the preparation of plasmid DNA, but I suspect…
Read More6 ways to show your plasmid preps some TLC and get more supercoiled plasmid in return
preps. Which is not good. Not good at all. And if the prep goes spectacularly wrong then you can end up with a prep full of the single-stranded stuff, which…
Read MoreIt’s Like Getting RNA From a Blood Sample
…purification kit ($8.12 a prep) . An alternative kit for processing PAXgene Blood RNA Tube collected samples exists. However, because of the difficulty in handling this sample, standard RNA prep…
Read More BioChemWeb: Virtual Library for the Molecular Biologist
…the Cell, Harvey Lodish et al.’s Molecular Cell Biology, and David Nelson’s Lehninger Principles of Biochemistry if you have the textbooks handy. An online compilation or virtual library is extremely…
Read More Useful Tips to Keep on Top of New Literature
…on what is already in your library and what you’ve recently read. These recommendations are available directly through ReadCube making it simple to add them straight to your library. CiteULike…
Read More RNAseq Library Preparation: From Cells to cDNA
…(next generation) sequencing of cDNA 5) in silico sequence alignment1, 2. This article is about the two first stages of making an RNAseq library (steps 1- 3). Starting Material Preparation…
Read More The Cell: An Image Library-CCDB – Tutorial Part 4
…the cell. The use of these ontologies, in addition to allowing for greater ease of collaboration with other groups using the same ontologies, are also in place to prepare The…
Read MoreProteomics and Good Mass Spectrometry Data
…if not removed prior to sample prep. Whew! That was a lot. Maybe most importantly, you should have everything set up for sample prep day. And plan your experiments carefully…
Read MorePractical Super-Resolution Imaging: SIM Capabilities, Sample Prep and Analysis
In the webinar you will learn: The background of structured illumination microscopy (SIM). Advantages of SIM. Sample preparation for SIM. How to avoid reconstruction artefacts. Abstract: Structured illumination microscopy (SIM)…
Read More Nanopore Sequencing: An Update
…and I got an update from multiple users at the UK Genome Science conference. Library prep methods are pretty standard: DNA, frag, end repair, A-tail, adapter ligation. Sequencing accuracy is…
Read More NGS-Based HLA Typing Delivers More Comprehensive Information
…size selection is an essential step in library preparation for consistently generating reliable results. HLA Typing Using NGS Requires DNA Size Selection for Library Preparation In the protocol released in…
Read More Battle of the Bargains: A Comparison Between Genome Compiler and ApE
…your own files and those provided in the Genome Compiler library. ApE does have an inventory of enzymes to choose from, but no sequence library, and finding the enzyme selector…
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