Search results for: library prep
WGS Workflow: From Sample Collection to Data Interpretation
…prep kit for Illumina systems make it possible for library generation to keep pace with instrument speeds. The Collibri PS DNA Library Prep Kit requires only 0.4 hour hands-on time…
Read MoreGet Prepped: Nanopore Library Preparation Optimization
…and Nanodrop measurements. Nanopore library quality mostly depends on the individual researcher’s technical skills and pipetting technique. In this article, we discuss some pointers to perform a successful NGS library…
Read MoreHow to Improve Your WGS DNA Library
…Library Prep Kit for Illumina Systems and Invitrogen™ Collibri™ PS DNA Library Prep Kit for Illumina Systems, it is possible to reduce these biases. References Macintyre G, Yistra B, and…
Read MoreLevel Up Your Drug Screening With CRISPR
…approaches. © 2015 Agrotis and Ketteler [5], licensed under CC BY 4.0. MOI, multiplicity of infection; sgRNA, single guide RNA. Pooled Library Screens A pooled library can include thousands of…
Read MoreThe Recipe for Successful Whole Genome Sequencing
…to obtaining high-quality WGS results and is available exclusively from the Invitrogen™ Collibri™ PS DNA Library Prep Kit and the Invitrogen Collibri PCR-Free PS DNA Library Prep Kit for Illumina…
Read MoreTop 10 Tips for NGS Library Preparation
Making a Next Generation Sequencing (NGS) library can seem a bit daunting to the new user, as failures can be expensive. But don’t be put off, as NGS library preparation…
Read MoreHow To Get Organized With Reference Managers for Science—Mendeley
…to your Mendeley library. This saves time and means that your library is effortlessly maintained and that you won’t lose that one vital paper that may hold the key to…
Read MoreCryo-EM Sample Prep: 5 Crucial Considerations
…sample prep workflow (Figure 1). Figure 1. Cryo-EM sample prep workflow. (Image credit: Thomas Warwick.) Figure 1 gives you an overview of the whole sample preparation process, how they are…
Read MoreHow To Troubleshoot Your DNA Libraries For 454 Next Generation Sequencing- An Essential Guide.
…Short pieces of DNA Short pieces of DNA left over from the library prep process can interfere with the emulsion PCR. Small products bind to the capture bead preferentially, competing…
Read MoreA Primer on Phage Display Libraries
…In a semi-synthetic library, these framework regions are amplified and cloned into the phage display library from B-cells, in differing combinations to achieve different goals. In a synthetic library, these…
Read More8 Approaches to Random Mutagenesis
…then randomly introduce mutations into the gene sequence to create a “library” containing thousands of versions of the gene. Each version (or “variant”) of the gene in the library would…
Read MoreZotero Reference Manager: A Super Simple Guide for Busy Researchers
…library of publications, collaborate easily with colleagues, and seamlessly insert citations in your chosen word processor. Maybe even more importantly, gone are the days of manually writing and formatting your…
Read MoreA Colorful Route to Sequencing Success
…Collibri PS DNA Library Prep Kit for Illumina systems provide visual feedback that allows real-time tracking of the successful mixing of reagents at each step in the library preparation and…
Read MoreWhere Did It All Go Wrong?! Quality Control For Your NGS Data
…If this number is high in your library it can indicate over-amplification or poor library–prep. (3) MiSeq QC: While not strictly a tool to check data from your own genome…
Read MoreWhere Did It All Go Wrong?! Quality Control For Your NGS Data
…If this number is high in your library it can indicate over-amplification or poor library–prep. (3) MiSeq QC: While not strictly a tool to check data from your own genome…
Read MoreHow To Get Organized With Reference Managers for Science—ReadCube Papers
…such as: library generation for your references; cloud syncing of your library; access across devices; and the ability to make notes, highlight text, and annotate PDFs. In addition, the sleek…
Read MoreThe Cell: An Image Library-CCDB – Tutorial Part 3
…account and can be found here https://bitesizebio.com/13416/the-cell-an-image-library-ccdb-tutorial-part-1/. The second tutorial described various ways to interact and find cell images and can be found here http://microscopy.bitesizebio.com/articles/the-cell-an-image-library-ccdb-tutorial-part-2/. In this tutorial you will…
Read MoreHow to Access Journal Articles Behind Paywalls
…library has the right issue of the journal. Then, call your institution’s or city’s library and arrange for a loan from that other library. This may cost a nominal fee…
Read MoreBetter Plasmid Purification: 11 Reasons Your Plasmid Yield is Low
…for plasmid preps. Prep type Plasmid DNA recovered (μg) Mini prep 5–50 Midi prep 50–200 Maxi prep 200–1000 What Causes Low Plasmid Yields? We received a question from Bitesize Bio…
Read MoreThe Real Cost of Sequencing in 2014
…post-doc in the lab. It’s about the same for RNA-seq of ChIP-seq prep as well. Cost for WGS library prep: $125 per sample. Illumina Sequencing Unlike library prep, sequencing is…
Read MoreThe Next Big Thing: Alternative Polyadenylation
…strand, single-stranded RNAs and RNA-DNA hybrids are removed by RNAses. The second cDNA strand is then synthesized by PCR using a poly(A)-anchored primer (PAAP). 1,4 Collibri RNA Library Prep Invitrogen…
Read MoreQuantifying Your NGS Libraries
…very high-throughput. Illumina recommends the very capable KAPA library quantification kit, but there are alternatives including Agilent, Qiagen and QuantBio, among others. The KAPA kit is a SYBR-green assay that…
Read MoreThe Cell: An Image Library – An Overview of an Award-winning Multimedia Site
Much more than just an archive, The Cell: An Image Library-CCDB (Cell Centered Database; ‘The Cell’) serves many additional purposes. Whilst many researchers use The Cell to organize their own…
Read More5 Ways to Really Screw Up Your RNA Prep
…I will discuss five mistakes that will no doubt hype your paranoia about the possible ways to screw up your RNA prep. 1. Not keeping the working environment RNase-free The…
Read MoreGenerating RNA-seq Libraries from RNA
…starts with size selection for small RNAs, ligation of selected fragments to adapters, and then generation of cDNA.4 New RNA Library Preparation Method A new RNA library prep method creates…
Read MoreAn Introduction to RNA-seq
…library prep which includes end-repair, adapter ligation and PCR amplification to give you a library ready for sequencing. Why Bother With Strand Information? There has been a lot of discussion…
Read MoreSolid Phase Reversible Immobilization: How To Get A Bead On The Clean-up Of Your NGS Libraries
…concerned with reproducible library preps, gel purification after adaptor ligation is the step that requires the most attention to detail (see James Hadfield’s excellent instructions of what you need to…
Read MoreAAV Production Part II: Virus Purification
In Part I of AAV Production, I described how to produce crude (non-purified) AAV. In this article, I am going to tell you how to purify that crude prep. Virus…
Read More6 ways to show your plasmid preps some TLC and get more supercoiled plasmid in return
…preps. Which is not good. Not good at all. And if the prep goes spectacularly wrong then you can end up with a prep full of the single-stranded stuff, which…
Read MoreIt’s Like Getting RNA From a Blood Sample
…purification kit ($8.12 a prep) . An alternative kit for processing PAXgene Blood RNA Tube collected samples exists. However, because of the difficulty in handling this sample, standard RNA prep…
Read MoreThe Cell: An Image Library-CCDB – Tutorial Part 4
…to yourself or save it in a document. Pasting that URL into the address bar of your browser and hitting enter will run the search against the library at that…
Read MoreRNAseq Library Preparation: From Cells to cDNA
…(next generation) sequencing of cDNA 5) in silico sequence alignment1, 2. This article is about the two first stages of making an RNAseq library (steps 1- 3). Starting Material Preparation…
Read MoreProteomics and Good Mass Spectrometry Data
…if not removed prior to sample prep. Whew! That was a lot. Maybe most importantly, you should have everything set up for sample prep day. And plan your experiments carefully…
Read MoreBeginners Guide to PALM Sample Preparation
…to start thinking about how to perform good PALM sample prep. In this article, we will cover how to choose which fluorescent proteins to use for PALM, and then go…
Read MoreScanning Electron Microscopy: 6 SEM Sample Preparation Pointers for Successful Imaging
…SEM sample prep points to maximize your chances of successful SEM imaging. Need to take a step back? Read our ‘Applications of Electron Microscopy‘ article to learn more about how…
Read MoreNanopore Sequencing: An Update
…and I got an update from multiple users at the UK Genome Science conference. Library prep methods are pretty standard: DNA, frag, end repair, A-tail, adapter ligation. Sequencing accuracy is…
Read MoreNGS-Based HLA Typing Delivers More Comprehensive Information
…size selection is an essential step in library preparation for consistently generating reliable results. HLA Typing Using NGS Requires DNA Size Selection for Library Preparation In the protocol released in…
Read MoreStudying the Epigenome by Next Generation Sequencing
…Thus, all that will remain is a library of fragments of methylated DNA, ready to be sequenced. Methyl-CpG binding domain protein sequencing (MBD-seq) The MBD family of proteins contains a…
Read MoreSix Important Factors for Successful Reverse Transcription
…gDNA, such as DNase treatments during an RNA Prep or DNase treatments after a prep (such as the Turbo DNA-Free Kit by Ambion). However, a better way to get around…
Read More5 Tips on Vector Preparation for Gene Cloning
…prep – you will likely regret it later. 2. Pay Attention to the Digest The digest looks easy on paper, but don’t underestimate the importance of good planning and execution…
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