Search results for: library prep
Improving Library Preparation for Next Generation Sequencing
of New England Biolab’s NEBNext® Ultra TM II DNA Library Prep Kit. Improving Library Preparation Quality Generation of high diversity libraries can be increasingly more difficult to achieve at lower…
Read More Thermo Fisher Scientific Expands Maybridge Ro3 Diversity Fragment Library
structure-based drug discovery. The new library adds 1,000 Ro3 (Rule of 3) compliant molecules, complementing the 1,500 compounds introduced last year. This expansion increases the diversity of the library by…
Read More How to Improve Your WGS DNA Library
…Library Prep Kit for Illumina Systems and Invitrogen™ Collibri™ PS DNA Library Prep Kit for Illumina Systems, it is possible to reduce these biases. References Macintyre G, Yistra B, and…
Read More Top 10 Tips for NGS Library Preparation
Making a Next Generation Sequencing (NGS) library can seem a bit daunting to the new user, as failures can be expensive. But don’t be put off, as NGS library preparation…
Read More How To Troubleshoot Your DNA Libraries For 454 Next Generation Sequencing- An Essential Guide.
For those of you who prepare your own DNA libraries, this article will cover the most critical aspects of library preparation to ensure a successful sequencing run. Previous Bite Size…
Read MoreA Primer on Phage Display Libraries
…molecule. In a semi-synthetic library, these framework regions are amplified and cloned into the phage display library from B-cells, in differing combinations to achieve different goals. In a synthetic library,…
Read More8 Approaches to Random Mutagenesis
…then randomly introduce mutations into the gene sequence to create a “library” containing thousands of versions of the gene. Each version (or “variant”) of the gene in the library would…
Read More Too good to be true?! What can Nextera do for you?
Read on… Nextera is an Illumina NGS library preparation system that works very differently from the standard fragment library preps being used in most labs. Instead of the usual Illumina…
Read MoreNext Generation Sequencing Library Preparation: Concepts, Tips and Tricks
can require different library construction strategies. We will show different strategies for NGS library preparation, such as primer designed panels, fusion primers, and whole genome library preparation— among others. Good…
Read More From the Lab to the Library…
…list. So how did I get this job? I did indeed go back to school to get my Masters in Library Science (MLS).Then I looked at library and university online…
Read More The Cell: An Image Library-CCDB – Tutorial Part 5
here https://bitesizebio.com/articles/the-cell-an-image-library-ccdb-tutorial-part-2/. The third tutorial showed how to use the Advanced Search feature to further pinpoint images and can be found here https://bitesizebio.com/articles/the-cell-an-image-library-ccdb-tutorial-part-3/. The fourth tutorial further explained licensing issues…
Read MoreWhere Did It All Go Wrong?! Quality Control For Your NGS Data
…If this number is high in your library it can indicate over-amplification or poor library–prep. (3) MiSeq QC: While not strictly a tool to check data from your own genome…
Read MoreWhere Did It All Go Wrong?! Quality Control For Your NGS Data
…If this number is high in your library it can indicate over-amplification or poor library–prep. (3) MiSeq QC: While not strictly a tool to check data from your own genome…
Read More The Cell: An Image Library-CCDB – Tutorial Part 3
account and can be found here http://microscopy.bitesizebio.com/articles/the-cell-an-image-library-ccdb-tutorial-part-1/. The second tutorial described various ways to interact and find cell images and can be found here http://microscopy.bitesizebio.com/articles/the-cell-an-image-library-ccdb-tutorial-part-2/. In this tutorial you will…
Read MoreHow To Get Organized With Reference Managers for Science – Mendeley
…references. For those working with Microsoft Word or Open Office, Mendeley offers plug-ins for easy insertion of citations from your Mendeley library as you write. Keeping track of your library…
Read More The Real Cost of Sequencing in 2014
…or post-doc in the lab. It’s about the same for RNA-seq of ChIP-seq prep as well. Cost for WGS library prep: $125 per sample. Illumina Sequencing Unlike library prep, sequencing…
Read More The Next Big Thing: Alternative Polyadenylation
…(Thermo Fisher Scientific) has developed a kit for the preparation of cDNA libraries for strand-specific RNA sequencing called Invitrogen™ Collibri™ Stranded RNA Library Prep Kit for Illumina™ with Collibri™ Human/Mouse/Rat…
Read More The Cell: An Image Library – An Overview of an Award-winning Multimedia Site
Much more than just an archive, The Cell: An Image Library-CCDB (Cell Centered Database; ‘The Cell’) serves many additional purposes. Whilst many researchers use The Cell to organize their own…
Read MoreHow to get Organised With Reference Managers for Science- Zotero
…download it to your library as well. However, Zotero doesn’t come with its own PDF viewer, so you can’t annotate or highlight articles in your library. Instead articles will open…
Read More Quantifying Your NGS Libraries
…very high-throughput. Illumina recommends the very capable KAPA library quantification kit, but there are alternatives including Agilent, Qiagen and QuantBio, among others. The KAPA kit is a SYBR-green assay that…
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