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How to Improve Your WGS DNA Library

Library Prep Kit for Illumina Systems and Invitrogen™ Collibri™ PS DNA Library Prep Kit for Illumina Systems, it is possible to reduce these biases. References Macintyre G, Yistra B, and…

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Level Up Your Drug Screening With CRISPR

…arrayed approaches. © 2015 Agrotis and Ketteler [5], licensed under CC BY 4.0. MOI, multiplicity of infection; sgRNA, single guide RNA. Pooled Library Screens A pooled library can include thousands…

Image of a microplate use to show how CRISPR can be scaled to undertaking drug screening.Read More

Top 10 Tips for NGS Library Preparation

Making a Next Generation Sequencing (NGS) library can seem a bit daunting to the new user, as failures can be expensive. But don’t be put off, as NGS library preparation…

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A Primer on Phage Display Libraries

…molecule. In a semi-synthetic library, these framework regions are amplified and cloned into the phage display library from B-cells, in differing combinations to achieve different goals. In a synthetic library,…

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8 Approaches to Random Mutagenesis

…then randomly introduce mutations into the gene sequence to create a “library” containing thousands of versions of the gene. Each version (or “variant”) of the gene in the library would…

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A Colorful Route to Sequencing Success

…Collibri PS DNA Library Prep Kit for Illumina systems provide visual feedback that allows real-time tracking of the successful mixing of reagents at each step in the library preparation and…

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The Cell: An Image Library-CCDB – Tutorial Part 3

…account and can be found here http://microscopy.bitesizebio.com/articles/the-cell-an-image-library-ccdb-tutorial-part-1/. The second tutorial described various ways to interact and find cell images and can be found here http://microscopy.bitesizebio.com/articles/the-cell-an-image-library-ccdb-tutorial-part-2/. In this tutorial you will…

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The Real Cost of Sequencing in 2014

…or post-doc in the lab. It’s about the same for RNA-seq of ChIP-seq prep as well. Cost for WGS library prep: $125 per sample. Illumina Sequencing Unlike library prep, sequencing…

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The Next Big Thing: Alternative Polyadenylation

…(Thermo Fisher Scientific) has developed a kit for the preparation of cDNA libraries for strand-specific RNA sequencing called Invitrogen™ Collibri™ Stranded RNA Library Prep Kit for Illumina™ with Collibri™ Human/Mouse/Rat…

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Quantifying Your NGS Libraries

…very high-throughput. Illumina recommends the very capable KAPA library quantification kit, but there are alternatives including Agilent, Qiagen and QuantBio, among others. The KAPA kit is a SYBR-green assay that…

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5 Ways to Really Screw Up Your RNA Prep

…target and degrade RNA. In this article, I will discuss five mistakes that will no doubt hype your paranoia about the possible ways to screw up your RNA prep. 1….

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Generating RNA-seq Libraries from RNA

…starts with size selection for small RNAs, ligation of selected fragments to adapters, and then generation of cDNA.4 New RNA Library Preparation Method A new RNA library prep method creates…

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