Search results for: library prep
WGS Workflow: From Sample Collection to Data Interpretation
…prep kit for Illumina systems make it possible for library generation to keep pace with instrument speeds. The Collibri PS DNA Library Prep Kit requires only 0.4 hour hands-on time…
Read More Get Prepped: Nanopore Library Preparation Optimization
…can reuse the flow cells for at least three library preps making it more economical compared to other sequencing platforms. Common Practices to Keep in Mind for Nanopore Library Preparation…
Read More How to Improve Your WGS DNA Library
…Library Prep Kit for Illumina Systems and Invitrogen™ Collibri™ PS DNA Library Prep Kit for Illumina Systems, it is possible to reduce these biases. References Macintyre G, Yistra B, and…
Read More Level Up Your Drug Screening With CRISPR
…arrayed approaches. © 2015 Agrotis and Ketteler [5], licensed under CC BY 4.0. MOI, multiplicity of infection; sgRNA, single guide RNA. Pooled Library Screens A pooled library can include thousands…
Read More The Recipe for Successful Whole Genome Sequencing
…to obtaining high-quality WGS results and is available exclusively from the Invitrogen™ Collibri™ PS DNA Library Prep Kit and the Invitrogen Collibri PCR-Free PS DNA Library Prep Kit for Illumina…
Read More Top 10 Tips for NGS Library Preparation
Making a Next Generation Sequencing (NGS) library can seem a bit daunting to the new user, as failures can be expensive. But don’t be put off, as NGS library preparation…
Read More How To Troubleshoot Your DNA Libraries For 454 Next Generation Sequencing- An Essential Guide.
For those of you who prepare your own DNA libraries, this article will cover the most critical aspects of library preparation to ensure a successful sequencing run. Previous Bite Size…
Read MoreA Primer on Phage Display Libraries
…molecule. In a semi-synthetic library, these framework regions are amplified and cloned into the phage display library from B-cells, in differing combinations to achieve different goals. In a synthetic library,…
Read More8 Approaches to Random Mutagenesis
…then randomly introduce mutations into the gene sequence to create a “library” containing thousands of versions of the gene. Each version (or “variant”) of the gene in the library would…
Read More Too good to be true?! What can Nextera do for you?
Read on… Nextera is an Illumina NGS library preparation system that works very differently from the standard fragment library preps being used in most labs. Instead of the usual Illumina…
Read MoreA Colorful Route to Sequencing Success
…Collibri PS DNA Library Prep Kit for Illumina systems provide visual feedback that allows real-time tracking of the successful mixing of reagents at each step in the library preparation and…
Read More Where Did It All Go Wrong?! Quality Control For Your NGS Data
…If this number is high in your library it can indicate over-amplification or poor library–prep. (3) MiSeq QC: While not strictly a tool to check data from your own genome…
Read MoreWhere Did It All Go Wrong?! Quality Control For Your NGS Data
…If this number is high in your library it can indicate over-amplification or poor library–prep. (3) MiSeq QC: While not strictly a tool to check data from your own genome…
Read More The Cell: An Image Library-CCDB – Tutorial Part 3
…account and can be found here http://microscopy.bitesizebio.com/articles/the-cell-an-image-library-ccdb-tutorial-part-1/. The second tutorial described various ways to interact and find cell images and can be found here http://microscopy.bitesizebio.com/articles/the-cell-an-image-library-ccdb-tutorial-part-2/. In this tutorial you will…
Read More The Real Cost of Sequencing in 2014
…or post-doc in the lab. It’s about the same for RNA-seq of ChIP-seq prep as well. Cost for WGS library prep: $125 per sample. Illumina Sequencing Unlike library prep, sequencing…
Read More The Next Big Thing: Alternative Polyadenylation
…(Thermo Fisher Scientific) has developed a kit for the preparation of cDNA libraries for strand-specific RNA sequencing called Invitrogen™ Collibri™ Stranded RNA Library Prep Kit for Illumina™ with Collibri™ Human/Mouse/Rat…
Read More The Cell: An Image Library – An Overview of an Award-winning Multimedia Site
Much more than just an archive, The Cell: An Image Library-CCDB (Cell Centered Database; ‘The Cell’) serves many additional purposes. Whilst many researchers use The Cell to organize their own…
Read MoreQuantifying Your NGS Libraries
…very high-throughput. Illumina recommends the very capable KAPA library quantification kit, but there are alternatives including Agilent, Qiagen and QuantBio, among others. The KAPA kit is a SYBR-green assay that…
Read More 5 Ways to Really Screw Up Your RNA Prep
…target and degrade RNA. In this article, I will discuss five mistakes that will no doubt hype your paranoia about the possible ways to screw up your RNA prep. 1….
Read MoreGenerating RNA-seq Libraries from RNA
…starts with size selection for small RNAs, ligation of selected fragments to adapters, and then generation of cDNA.4 New RNA Library Preparation Method A new RNA library prep method creates…
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