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Optical spectroscopy is a non-destructive technique that uses light to analyze biological samples via absorbance, transmission, and photoluminescence. Check out this article to learn more about how it works and its applications across biology.
Glucose repression, leaky expression, and arabinose catabolism can all hinder protein expression. This short guide introduces the pBAD promoter and gives you some advice on using it for consistent protein yields for your studies.
Unsure which next-generation sequencing (NGS) method to choose for your cancer and precision medicine research? Learn about the latest tools and tailored next-generation sequencing solutions for cancer research in this short guide.
Plasmid maps are a cornerstone of biology, but they are confusing to read for beginners. Our easy guide tells you how to read them, where to look for essential information, and how to avoid common mistakes.
Explore the pros and cons of key DNA Cloning techniques such as TA, Golden Gate, and Gateway cloning, and find out how to manage DNA complexities in our guide to Molecular Cloning.
Achieving publication-quality immunofluorescence images is tricky. Learn what controls for immunofluorescence you can use to get them!
Protein crystals are crucial for structure solution via X-ray crystallography but are notoriously hard to grow. We’ve got you covered with 5 clever protein crystallization seeding methods to grow impressive protein crystals.
Follow our simple lentiviral transduction protocol and discover expert tips and tricks to make your transductions a success.
Machine learning is making a massive impact on the life sciences and can now help deliver fast and accurate automatic hepatocyte counts. This article explains hepatocyte counting methods, from manual counting to the latest AI-assisted techniques.
Mass spectrometry molecular separation via mass/charge (m/z) ratios. Each of the various ionization approaches excels in targeting different molecule types. Understanding these techniques can help you pick the optimal ionization method to “make your molecules fly” and reveal their unique mass spectra. Read this article to learn more.
Working with membrane proteins can be tricky. Read on to get tried-and-tested tips and tricks for expressing, extracting, and purifying these water-phobic proteins.
Fat and blurry bands on your SDS-PAGE gel can leave your experimental question unanswered. Learn how you can sharpen up your bands and get much greater resolution by using Bis-Tris gels.
Discover how digital ELISAs revolutionize protein detection with unparalleled sensitivity. This article explores cutting-edge technologies like Simoa that detect multiple biomarkers from small samples, transforming research in areas like neurology and immunology.
Metabolic pathways include all the chemical reactions in an organism that sustain biological function, and metabolic tracing lets us study them. This article explains what metabolic tracing is, the isotopes used for different types of study, and how to incorporate isotopes into samples.
Are you preparing to run your first column chromatography experiment? Here are some basics that you should know before you use that precious sample.
Hydrogen bonds are critical non-covalent bonds essential for the structural integrity and function of almost all biological molecules, particularly DNA and proteins. This article explains the fundamental chemistry of hydrogen bonds and nomenclature and highlights why they are critical for life.
This article explains a simple, 4-step method for automatic cell counting with ImageJ. Perfect for your cell proliferation studies, gene expression analysis, or whatever your downstream application might be.
Learn how UV radiation causes DNA mutations. Read about about the mechanisms that lead to skin cancer and the detrimental effects that UV light can have on our DNA-based experiments.
Using ethanol precipitation to isolate or concentrate your nucleic acids? Find out how this routinely used technique works, and get tips to produce the best results.
Discover top strategies for optimizing nuclei extraction and learn how to adapt protocols for improved single-cell sequencing outcomes.
Navigating the complexities of microbiome data visualization can be challenging. Our guide offers insights on selecting the right plots and optimizing them for maximum readability and publication readiness.
While CRISPR offers vast applications in disease research and drug target identification, it’s not always the optimal choice for every scenario. Explore the main advantages and challenges of using CRISPR-Cas9 to determine if it’s the right fit for your project.
Tissue processing for histology is a key step between fixation and embedding. We take you through the steps of tissue processing in this simple guide.
Agar plates are the foundation of many experiments. Make sure your plates are perfect every time with these 8 tips and start your experiment ready for success!
Nucleic acid extraction kits are routinely used in today’s molecular biology labs. Read on to learn more about what is inside these black boxes of wonder and how you can get the best results for your preps.
Biomarkers are fundamental in bioscience, from basic microbiological experiments to clinical studies. This article explains the different types of biomarkers and their application in cancer and disease research.
Adherent cell fixation is a crucial step in preparing cells for microscopy and imaging, ensuring that cellular structures are preserved for detailed analysis. Read our 8-step guide on how to effectively fix adherent cells to your microscope slides, including tips on sterilization, coating, and fixation methods, right here.
Isoelectric focusing is a way of separating proteins and peptides based on their isoelectric point. Read all about this method and its applications right here.
Ever wondered what magic happens to turn your samples into histology slides? Find out the 5 simple steps for histology slide preparation.
Learn how the CRISPR prokaryote immune response systems were first discovered and the development of the CRISPR-Cas9 gene-editing tool.
Don’t be confused by Ct values! We’ll guide you through what they are, how to calculate them, and troubleshooting issues.
In-cell Westerns are a powerful technique that has enhanced how researchers analyze protein expression levels and signaling pathways within fixed cells. Learn about their primary advantages, applications, and some of the best tech and products to perform them.
Selecting the right blood collection tubes for your experiment is crucial. But do you know what tubes to use for which type of blood sample? Read on to find the answers.
The emergence of Volume Electron Microscopy (vEM) has unlocked new possibilities in biological imaging, enabling us to visualize 3D structures of cells at high resolution. Learn more about this incredible technique in our latest article.
Discover what hematoxylin and eosin staining is used for and how it works, in this concise guide.
Purified proteins must be kept soluble and active. Discover how to design the perfect protein purification buffer—from additives you can include and their appropriate concentrations to important considerations.
Most experiments start with a piece of DNA—either plasmid DNA or genomic DNA. And your downstream uses for it dictate how much you need, what contaminants you can tolerate, and your extraction and purification methods. In this article, we explain the key differences between plasmid and genomic DNA extraction methods.
Do you prepare samples for electron microscopy and want to save time, money, effort, and frustration? This article provides hands-on advice to help you get the best possible data out of your EM experiments.
Need to add extra nucleotides to your plasmid or other DNA sample? Here’s how to use overhang PCR to easily add extra bases using primers.
Investigating human diseases and genetic variation is complex, but CRISPR-edited induced pluripotent stem cells present a promising alternative to immortalized cell lines. This article delves into genome editing principles and offers practical steps for optimizing research techniques, ensuring more accurate and ethical studies.
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