" The Missing Manual For Bioscientists "

Transfection Toolkit

By Chelsey Kline | May 24, 2017

Engineering a mutation or overexpressing a recombinant protein to study and characterize its function in mammalian cells is no easy task. Luckily, Chinese hamster ovary (CHO) cells, which have been a mainstay in the lab since the 1950s, represent a relatively easy mammalian model system to engineer. There are several methods to choose choose from…

Read More

Need a Few More Hours in the Day? Here Are Some Timesaving Tips

By Melissa Walker | May 24, 2017

Are you constantly looking for ways to squeeze more lab hours out of the day? Here are some timesaving tips that can help. Figure out Where Your Time Goes Not sure where you are losing those valuable hours? Use a website like Toggl to help you keep track of your time. This will make it…

Read More

The 3 Most Common Flow Cytometry Fallacies

By Kristen Haberthur | May 23, 2017

Flow cytometry is fast evolving from a method only revered by immunologists, to one used by nearly every biological specialty. It’s pretty much my favorite tool. Unfortunately, as with most lab techniques, much of flow cytometry is taught on the job without a lot of standards. And too often bad habits are passed along like…

Read More

Don’t Let Bubbles Burst Your Experimental Excitement

By Chelsey Kline | May 22, 2017

Bubbles isn’t just the name of my favorite cartoon character from Power Puff girls, or just the best activity for a kid to play with, in general. In my adult world, they stand for a whole lot more, but can still cause extreme emotions. At the lab bench, seeing bubbles brings happiness or sadness depending…

Read More

Explained: Sensor Chips for Surface Plasmon Resonance and Other Applications

By Reichert Technologies | May 19, 2017

Biosensor chip selection is a critical step in planning and running a surface plasmon resonance (SPR) experiment. Chip selection depends on the ligand or target that needs to be immobilized on the sensor chip, the analyte that is flowed over the target to study the binding, and the purpose of the biosensor assay (i.e., determination…

Read More

Get Your Polymerase Cycling Assembly Oligos Together

By Kristen Haberthur | May 18, 2017

The polymerase chain reaction (PCR) is the backbone of many lab techniques. In short, it allows for the exponential amplification of a specific segment of DNA. Through the use of primers encoding restriction enzyme sites, these amplified fragments are used in downstream cloning procedures, usually leading to the insertion of one, maybe two, PCR fragments…

Read More

Simple Tips for Model Organism-Based Work

By Uma Chandrasekaran,PhD | May 17, 2017

Simple Tips for Model Organism-Based Work The mouse is the favored model organism for life science researchers so much so that mice account for about 95% of all lab animals used in research. The striking similarities between the human and mouse genomes, ease of genetic manipulation and the uniformity achieved through inbred mating makes them…

Read More

Detecting Cell Apoptosis on Tissue Slides

By Ilona Marecko | May 17, 2017

Apoptosis, or programmed cell death, is a physiological process in which individual cells are set to die without harming their environment. It involves a cascade of complex and tightly regulated cellular events. Detecting apoptosis on tissue slides, involves either detecting the molecular participants of these events, or the morphological changes that occur on the cellular…

Read More

Probability Theory and Molecular Barcodes

By Claudia Rabuffo | May 16, 2017

In biology, a molecular barcode is a characteristic DNA sequence used to distinguish and gather together similar items. Such a simple but powerful concept is useful in various applications. As an example, the Barcoding of Life project aims to identify specimens through the sequencing of standard gene regions, and use these as barcodes. On the other…

Read More

How to Perform DNA Extraction from Dried Blood Spots Using Chelex Resin

By John Ategeka | May 16, 2017

Every bio- scientist who wants to analyze DNA knows that the process begins with the extraction of DNA from cells of interest. These cells could be RBCs, parasites, or bacteria to name a few. Furthermore, there are various DNA extraction methods1  to choose from depending on sample type, downstream analysis, and so forth. Many scientists…

Read More

Coming Soon

Bitesize Bio All New Membership.

You can see we have rebuilt our website and we are also rebuilding our subscriber membership area. Like all good things it takes time.

Bioscience Mastery Academy

Bioscience Mastery Academy is a focused skills program that will speed your development into a happier, wiser and more successful scientist