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Molecular Docking: Let the Docking Do the Talking

Want to know whether a lead molecule or a ligand of your choice interacts with a particular protein or a receptor? Do you want this information at your fingertips? All it takes is just a few clicks and key...
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Getting Through Graduate School: Advice from Beyond the Battlefield

“If you could go back in time, would you do it again?” It’s a question I’ve been asked more times than I can remember. If I knew what getting a PhD entailed, would I still have gone for it? I wish I...
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Article

Show Disparity in Gene Expression with a Heat Map

Have RNA-seq or microarray data? What possible tools can help you find your genes of interest? Is there any pattern in your expression data? I know you are totally at sea but heat maps are now commonly used to...
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Are Quantum Dots Any Good for Flow Cytometry?

What Are Quantum Dots? Quantum dots were discovered in the early 1980s. However, it was not until the late 1990s that their use in biological applications was suggested.1 Quantum dots are semiconducting...
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Five Reasons Why You Should Have Hobbies While in Graduate School

You’ve chosen a career in science and gone off to graduate school because you love doing it. For you, lab is home. You are so used to wearing your lab coat all the time that when you go to the kitchen to boil...
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In Situ Zymography: Let’s Catch that Enzyme in Action!

In situ zymography (ISZ) is the best choice to study proteases. Proteases are a challenge to study as proteases are extremely potent enzymes. As such, they need to be controlled at multiple levels to prevent...
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Should I Stay or Should I Go? Part II: How to Gracefully Exit a Graduate School Program

This is Part II in the “Should I Stay or Should I Go?” series. Part I focused on how to evaluate whether staying in graduate school is a good idea for you. In this article, I’ll discuss the logistical...
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Titering Phage – Counting Something Invisible with Something Only Slightly More Visible

Titering Phage - The Plaque Assay Phage display is a molecular technique used to isolate binding or interaction partners to molecules of interest from an extensive library. Such libraries are often derived...
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Article

5 Things to Remember as a Research Intern

Make Friends as a Research Intern I used to be a supporter of the ‘lone wolf’ stance. Who needs friends or enemies? I’m completely capable of doing things on my own. Wrong and wrong again. This is...
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Article

How to perform cell synchronization in specific cell cycle phases

The cell cycle has been very well documented over the years because of its dysregulation in diseases such as cancer. Many different processes contribute to cell growth and replication, which is ultimately...
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