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Dr Jennifer Redig

I have a Masters of Clinical Research, and a PhD in Molecular & Medical Genetics. However I love keeping up with a wide variety of scientific topics – making my work as a Managing Editor at BitesizeBio very enjoyable.

I am passionate about academic reform and being a mom. Connect with me on Twitter and Facebook!

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Articles by Jennifer Redig

Image of a girl surround by traps to represent avoiding histology mistakes

Avoid These Pitfalls: Seven (Not So Deadly) Histology Sins

By Dr Jennifer Redig | August 2, 2021

Discover seven common histology mistakes and how you can avoid making them when performing your experiments.

A gloved hand inserting PCR tubes into a thermocycler to represent answering the question how to get started with PCR

What is PCR? The Beginner’s Guide

By Dr Jennifer Redig | May 13, 2021

If you need to copy, sequence, or quantify DNA, you need to know about PCR. Read our guide to the PCR process, and discover tips to help you avoid the most common PCR pitfalls.

Image of a lumberjack cutting a tree to represent tissue sectioning tips

Advanced Sectioning Techniques: How to Section Difficult Tissues

By Dr Jennifer Redig | March 5, 2021

Are you having problems with tissue sectioning? Follow these 10 tissue sectioning tips to create the perfect tissue section every time without stressing out.

Slides 101

Slides 101

By Dr Jennifer Redig | March 31, 2015

Would you eat your spaghetti dinner without a plate? No, of course not! It would make a big mess and be ugly to look at. Instead you NEED something to put that spaghetti on, to contain it, to keep it clean, to make it look nice – a bowl, a plate, SOMETHING! By the same…

Clean Up Your Act! How To Clean Up PCR Contamination

Clean Up Your Act! How To Clean Up PCR Contamination

By Dr Jennifer Redig | January 1, 2015

The biggest source of PCR contamination is aerosolized PCR products. How do you fix PCR contamination and avoid it in the future?

Digital Pathology – why you need it and how to choose the best camera for it

Digital Pathology – why you need it and how to choose the best camera for it

By Dr Jennifer Redig | October 30, 2014

Yup, we really are in the digital age…even the pathology is digital. Facebook, Instagram, Tumblr. It has never been easier to take pictures and share them. The digital revolution is upon us and nothing is safe, even your pathology samples. Digitizing your pathology samples can help you better organize and manage pathology for later. And…

The Devil is in the Details: How to Setup a PCR Laboratory

The Devil is in the Details: How to Setup a PCR Laboratory

By Dr Jennifer Redig | August 1, 2014

There is a right way and a wrong way to set up a PCR laboratory. Because of PCR’s tremendous ability to amplify small quantities of DNA/RNA template, even the smallest of template contamination can become a huge problem in PCR. However, contamination does not have to be a problem in your laboratory. Read below to…

Keeping 'em Alive! Incubators and Chambers for Live-Cell Imaging

Keeping ’em Alive! Incubators and Chambers for Live-Cell Imaging

By Dr Jennifer Redig | May 6, 2014

Live-cell imaging is a powerful technique that allows you to image dynamic cell processes, such as protein trafficking, signal transduction, endocytosis, and exocytosis, etc. However, to perform your own live-cell imaging you need the right equipment, most notably a live-cell imaging chamber or incubator. Keeping ‘em alive! Live-cell imaging differs from conventional imaging in that…

Just What Do All These Additives Do?

Just What Do All These Additives Do?

By Dr Jennifer Redig | April 4, 2014

Every PCR battle is the same: Too little amplification of your target DNA versus too much amplification of off-target DNA. But you can win the PCR battle and amaze your co-workers by mastering the use of PCR additives. PCR additives usually work one of two ways: 1) By reducing secondary DNA structures and thus increasing…

“Where the hell did my signal go?” AKA The Problems (and Uses) of ‘Photobleaching’ in Microscopy and Imaging

“Where the hell did my signal go?” AKA The Problems (and Uses) of ‘Photobleaching’ in Microscopy and Imaging

By Dr Jennifer Redig | March 4, 2014

Like most things in this world, fluorophores are mortal, and eventually your once bright fluorescent image will inevitably fade to black. This fading or ‘photobleaching’ of fluorescent signal can make imaging difficult, especially if you are trying to take quantitative images. Read below to learn what causes photobleaching of your fluorophores and how best to…

ELISA

Let me introduce you to ELISA…No, not the girl…The assay.

By Dr Jennifer Redig | March 4, 2014

An ELISA (Enzyme-Linked ImmunoSorbant Assay) is a popular assay that uses antibodies and color change to detect proteins, peptides, antibodies or biomolecules in complex mixtures. ELISAs are popular because they are reliable, specific, easy to use, and can easily be scaled up to process multiple samples simultaneously. How an ELISA is Done: In an ELISA,…

Welcome to the Dark Side…Dark Field Microscopy That Is!

Welcome to the Dark Side…Dark Field Microscopy That Is!

By Dr Jennifer Redig | February 25, 2014

We all know that stars are easier to see against the dark background of the night than they are to see against the bright sky of day. Well did you also know that the same may be true of your microscope specimen? Dark field microscopy is a popular microscope technique that makes your unstained transparent…

Image of pouring milk as it can be used for blocking non-specific staining

Immunohistochemistry Basics: Blocking Non-Specific Staining

By Dr Jennifer Redig | November 19, 2013

Achieving a good immunohistochemistry signal-to-noise ratio involves many factors, including a good blocking protocol. Read on to learn about blocking non-specific staining in IHC.

How To Fix Isolated And In Situ Primary Cells

By Dr Jennifer Redig | October 22, 2013

Unlike immortalized cells lines, primary cells can only be kept in cell culture for a finite period of time, if at all. Therefore, you often need to obtain primary cells directly from an animal source. After which, you may fix and image the primary cells in situ (as part of the whole organ), or you…

How To Fix Suspension Cells For Microscopy And Imaging

How To Fix Suspension Cells For Microscopy And Imaging

By Dr Jennifer Redig | October 15, 2013

Fixing suspension cells for imaging can be trickier than fixing adherent cells, as they can’t be cultured on a coverslip. Discover how you can stick them down with the help of centrifugation.

How To Fix Adherent Cells For Microscopy And Imaging

How To Fix Adherent Cells For Microscopy And Imaging

By Dr Jennifer Redig | October 8, 2013

Many cell lines commonly used in research are adherent, meaning they attach and grow on a surface rather than just hanging out in suspension. If you wish to perform imaging of adherent cells, such as to undertake cancer cell microscopy, you need to fix them to your microscope slides. Luckily for you- this is fairly easy…

An image of lab furniture to depict how not to wreck your autoclave.

Cell and Tissue Fixation 101- Top Tips For Protocol Optimization

By Dr Jennifer Redig | October 1, 2013

You just can’t put raw tissue or cell samples on your slides and expect good histology results! Instead you must preserve or ‘fix’ your samples. Fixing ensures that your cell structures stay intact and that your antigens are immobilized. Ideally, fixation would also still permit unfettered access of your antibodies to your antigens. However, as…

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