Shoba works at a biotech company in Wisconsin. She has MS from the University of North Carolina.

Articles by Shoba:

Touchdown PCR: A Primer and Some Tips

When I first heard of touchdown PCR, I thought of a landing aircraft, which, as it turns out is not a bad way to think about it. But despite it’s amenability to analogies and dreadful puns (see title), touch-down PCR (TD-PCR), a very useful technique for improving PCR amplification specificity, is trickier that it might…

09 Jul 2016 PCR, qPCR and qRT-PCR

10 Tips for Pipetting Perfection

Several years ago as a freshman in a research lab, the very first project I received was to pipette incremental micro-volumes of H2O onto a piece of parafilm. Boring! Weighing the liquid on parafilm and comparing the weight between 10 replicates for each micro-volume continued for a week before I touched anything else in that…

28 Nov 2011 Equipment Mastery & Hacks

Eliminate PCR Amplicon (but not real-time PCR?) Carry-Over With UNG

Using UNG is a great trick for PCR amplicon decontamination, but is it any good for rtPCR?

08 Oct 2010 PCR, qPCR and qRT-PCR

PCR: The Right Way to Decontaminate and Eliminate False Positives

PCR is highly sensitive, but the downside of that very property is that it makes the technique prone to producing false-positives. In labs where PCR is a staple, like the one I work in, any false-positives are more often than not due to amplicon contamination. A broken capillary or a PCR plate left carelessly at…

09 Apr 2010 PCR, qPCR and qRT-PCR

How to Build a Plate Centrifuge for $25

I recently visited a lab that had a salad spinner on their lab bench and at first I wondered if they were putting together a salad lunch there but when I took a peek I got a nice surprise. It turns out that the salad spinner was actually a bench top, “minifuge” version of a…

12 Mar 2010 Equipment Mastery & Hacks