Quantcast

SKennedy

Suzanne is Director of R&D at Mo Bio Laboratories in California, and the author of their blog, The Culture Dish. She has a PhD in Microbiology and Immunology from Virginia Commonwealth University.

Articles by SKennedy:

10 Tips For Better DNA Gel Extraction Results

What is it about DNA gel extraction that makes it a pain? Maybe it’s poor product yields or maybe it’s because the process uses harsh chemicals (chaotropic salts, ethidium bromide, ethanol, heat) that will damage or denature DNA and potentially decrease cloning success. In this article I share some tips, both from experience and from…

09 Jul 2016 DNA / RNA Manipulation and Analysis

The Key to Unlocking DNA from FFPE Tissues

Formalin fixed paraffin embedded (FFPE) tissues are valuable samples that typically come from human specimens collected for examination of the histology of biopsies for the detection of cancer. But each sample contains much more information just waiting to be unlocked. Despite the tiny sample size, DNA can be extracted from the tissue sections and used…

09 Jul 2016 Microscopy & Imaging

10 Tips for Consistent Real-Time PCR

Real-time PCR is a specialized technique that delivers far more information about your DNA or RNA than end-point PCR. Essentially, real-time PCR is a way to visualize the amplification of specific DNA fragments as it is happening (in real time) and allows for the ability to quantify exactly how much DNA (or RNA) was in…

09 Jul 2016 PCR, qPCR and qRT-PCR

There’s No Need To Be Paranoid About RNA Purification

RNA purification may be a common procedure in molecular biology but it is by far the one that people fear most. Why? Dreaded RNase. It’s everywhere… all over your bench and pipettors, and floating in the air, waiting for the chance to creep into your prep, shred your RNA into nucleotides, and ruin a day’s…

09 Jul 2016 DNA / RNA Manipulation and Analysis

No More White Elephants! – Consider this Before Buying a Real-time PCR Cycler

Does your lab have a closet full of white elephants; once expensive instruments that are no longer fit for purpose, or have broken down? In many cases, all of that wasted money and resource could have been saved if the buyers had made smart choices about matching the instrument more closely to their needs. A…

09 Jul 2016 PCR, qPCR and qRT-PCR
1 2 3