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Dr Nick Oswald

I started Bitesize Bio on a Macbook on my kitchen table in 2007 while in my 7th year of working as a molecular biologist in biotech. My aim was to share the know-how that I had acquired from the school of hard-knocks in the lab, so that others could learn from my mistakes and small victories. Nowadays my mission is to facilitate the gathering of hardcore know-how from whole spectrum of bioscientists and share it here on Bitesize Bio to create a super-mentor that any bioscientist can turn to for much-needed guidance.

Articles by Dr Nick Oswald:

What’s The Problem With Ampicillin Selection?

Resistance to the antibiotic ampicillin is commonly used as a selection marker for plasmids in gene cloning and protein expression in E.coli and other bacteria. While it can be incredibly useful tool, there can be problems using this selection marker that you need to be aware of if if you plan on using it. This…

17 Dec 2019 DNA / RNA Manipulation and Analysis

Antibiotics Used in Molecular Biology

Antibiotics are used in a wide range of techniques in molecular biology including molecular cloning and are important for treating pesky mycoplasma contamination in cell cultures. They can also be used to maximize your plasmid yeilds by reducing protein synthesis, in certain circumstances. The aim with this post is to provide an easy reference to…

10 Dec 2019 DNA / RNA Manipulation and Analysis

How to Avoid Accidental Plagiarism and Academic Fraud [BMA Podcast]

In the modern research environment we have all of the information we need to work with right at our fingertips – just a cut and paste away. And that makes it very easy for even the most well-meaning scientist to accidentally stumble into plagiarism. Most of us think of plagiarism as simply copying someone else’s…

16 Jun 2017 Writing, Publishing and Presenting

How to Cope With Overwhelm in the Lab: Taming Your Inbox

Look around you in your lab, your institution, and even in the world in general and you’ll see how much we all gravitate towards stress and overwhelm. Stress is just the workplace norm. Overwhelm means you are working “hard enough”. Being so occupied that you are frantically buzzing around from one task to another means…

05 Jun 2017 Organization and Productivity

The Easier Way to Write a PhD Thesis

Without good planning and preparation, writing your thesis can be a nightmare. Here are some tips on how to make the process a whole lot easier.

phd thesis
10 Aug 2016 Writing, Publishing and Presenting

The Essential PCR Troubleshooting Checklist

Routine PCR? Let’s be honest, there’s no such thing. Even with the simplest PCR reaction things can go wrong, so you need to have a good checklist of ideas for PCR troubleshooting and rectifying the problem. Today I have brainstormed all of the ways I can think of to approach problems with standard PCR reactions.…

27 Jul 2016 PCR, qPCR and qRT-PCR

E.coli Electroporation vs Chemical Transformation

This is the first in a three part series on the transformation of E.coli. By the end of this you should be an expert on E.coli transformation and on which strains to choose for different applications. If you’re already an expert, I hope it’ll be an enjoyable refresher for you. In either case, please comment…

26 Jul 2016 DNA / RNA Manipulation and Analysis

How SDS-PAGE Works

What does a stacking gel do? This and other questions about SDS-PAGE answered!

SDS PAGE
13 Jul 2016 Protein Expression and Analysis

Perfectionism: Are you on the downward spiral?

Do you fear failure every time you do an experiment? Do you feel constantly stressed about obtaining poor results? Do you feel personally culpable when an experiment goes wrong? If you answered “yes” to any or all of these questions, you may be suffering from perfectionism. For a scientist, this is a particularly damaging trait…

09 Jul 2016 Personal Development

5 DNA Ligation Tips

DNA ligations can be frustrating. Sometimes they don’t work for no obvious reason. Our top 5 DNA ligation tips should improve the efficiency of your ligations, and will hopefully increase your cloning success rate! 1. Aliquot the ligase buffer The ATP in the ligase buffer is essential for the DNA ligation reaction, but is broken down…

DNA Ligation Tips
09 Jul 2016 DNA / RNA Manipulation and Analysis

Top Tips to Keeping Your Logbook in Shipshape

Unless you are one of those rare breeds that do organization naturally, setting a system in place to archive your experiments takes practice and perseverance. It’s hard to imagine when you are doing an experiment for the 100th time that you will ever forget how to do it; but a year down the line, when…

09 Jul 2016 Organization and Productivity

How to Shut Off Background Lac Expression in LB

Here’s a tip that you may find useful if you are expressing proteins in E.coli using a lac promoter-based expression system, e.g. pET, in LB medium (L-broth). Lac expression systems are typically induced in the lab using IPTG (isopropyl-beta-D-thiogalacto- pyranoside), which is a non- hydrolyzable analog of lactose, the natural inducer of the lac operon.…

lac expression
09 Jul 2016 Protein Expression and Analysis

PCR Problems? Try an Additive

You’ve tried all the usual stuff, and checked the primer sequences twice, but still can’t get that PCR fragment amplified. It’s time to enter the strange world of PCR additives. Over the years a variety of additives have been shown to enhance PCR reactions in certain situations. Here is a summary of some of the…

09 Jul 2016 PCR, qPCR and qRT-PCR

Get Your Clone 90% Of The Time with Ligation Independent Cloning

Are you stuck in cloning hell?, Tired of doing ligations that don’t work? Want a faster, more efficient cloning procedure? You should try ligation independent cloning. A growing number of researchers swear by ligation independent cloning methods because they are simpler and more efficient than conventional cloning and as a recent convert to their ranks,…

09 Jul 2016 DNA / RNA Manipulation and Analysis