A Short History of Cryo-Electron Microscopy
The slow, inching progress of cryo-EM towards the scientific mainstream can be told as a story with three parts. So take a step back and enjoy a short history of cryo-electron microscopy.
Read MoreHow UV Light Damages DNA and the Havoc it Can Cause to Your Experiments
Learn how UV radiation causes DNA mutations and discover more about the mechanisms underlying skin cancer, as well as the detrimental effects that UV light can have on our DNA-based experiments.
Read MoreCryo-EM Sample Prep: 5 Crucial Considerations
You don’t have to be a brainbox to get your samples ready for cryo-EM, but a little wisdom goes a long way. Learn how to tend to your tissues, organize your organelles, and prepare your proteins to get the micrographs you’ve always dreamed of.
Read MoreThe 2 Main Electron Microscopy Techniques: SEM vs TEM
Microscopy is a huge and active field. Sometimes, it’s easy to forget the basics. Read our biologists’ guide to electron microscopy techniques.
Read MoreSustainable Research: 12 Simple Hacks to Reduce the Environmental Impact of Your Research
It’s a tough reality, but research can be damaging to the environment. Discover our simple tips and do more sustainable research.
Read MoreApplications of Electron Microscopy: An Easy Intro for Biologists
Microscopy is a huge and active field. Sometimes, it’s easy to forget the basics. Read our biologists’ intro to applications of electron microscopy.
Read More11 qPCR Papers Every Researcher Should Know
Whether you’re generating, analyzing, or reviewing qPCR data you need to understand how it works and best practices. That’s why we’ve pulled together our top qPCR papers every researcher should read.
Read MoreBetter Plasmid Purification: 11 Reasons Your Plasmid Yield is Low
Are your plasmid preps giving your poor yields? We’ve got 11 reasons that might be causing it and how to fix them.
Read MoreZotero Reference Manager: A Super Simple Guide for Busy Researchers
Organizing your files is simple with Zotero; we’ve got the lowdown on functionality, usability, and access.
Read MoreThe Importance of Delegation for Scientists
The art of delegation can help save time and give you a happy, more productive lab life. Discover how with our easy guide to delegation for scientists.
Read MoreThe Definitive Guide to pH, pKa, and pI
We all rely on pH, pKa, and pI for a vast amount of our research. But what is pH? How does it relate to pKa and pI? Read the definitive guide.
Read MoreProtein Structure Analysis: Extra Information in a Protein Data Bank File
Getting to know your protein’s structure can help uncover deeper insights and inspire new hypotheses. Discover how protein data bank files can help.
Read MoreHow Does Peer Review in Science Work?
If you’re unsure as to how the peer-review process works, the difference between open and double-blind peer review or are wondering if peer review is broken, read on.
Read MoreWhy Is SDS-PAGE Run Vertically? Here are 3 Great Answers
Why is SDS-PAGE run vertically? Agarose gel electrophoresis is run horizontally, so why not SDS-PAGE? Here are 3 great answers.
Read MoreStrengths and Limitations of the Amazing NanoDrop™ Spectrophotometer
With the NanoDrop spectrophotometer, quantifying a DNA, RNA, or protein sample concentration is as easy. Here’s everything you need to know about the strengths and limitations of this handy spec.
Read MoreTotal RNA Extraction: Easy Methods for Getting High-Purity RNA
Discover ways to perform total RNA extraction from TRIzol to kit-based and even old-school methods.
Read MoreAn Introduction to Circular Dichroism Part 2: Circular Dichroism Practice
Circular dichroism is a type of spectroscopy that can tell you the type and percentage of secondary structure units in a protein sample. Here are 5 handy pointers on best circular dichroism practice to set up your experiment properly.
Read MoreTop Tips for Getting Science Research Grants
Getting science research grants is becoming increasingly competitive and difficult. Read our top tips to maximize your chances of success.
Read MoreHow to Clean up Chemical Spills in the Lab: 4 Essential Rules
No matter how small or large, chemical spills require immediate attention. Learn how to manage spills by following these 4 essential rules.
Read MoreAn Introduction to Circular Dichroism Part 1: Circular Dichroism Theory
Circular dichroism is a type of spectroscopy that can tell you the type and percentage of secondary structure units in a protein sample. This article will take you through circular dichroism theory and some of its applications.
Read MoreA Simple SDS-PAGE Gel Recipe and 10-Step Casting Protocol for Perfect Gels
SDS-PAGE is the technique that unites life scientists. We all perform it during our research to separate protein analytes and, therefore, we all need a good SDS-PAGE gel recipe.
Read More5 Protein Crystallization Seeding Techniques for Bigger and Better Crystals
Protein crystals are crucial for structure solution via X-ray crystallography but are notoriously hard to grow. We’ve got you covered with 5 clever protein crystallization seeding methods to grow impressive protein crystals.
Read MoreHow Filtration Works: A Short Guide for Biologists
If you’ve worked in a lab, odds are you’ve had an encounter with filtration of one sort or another. Do you understand exactly how filters work, though? Or have you wondered why certain filters are used for certain lab applications?
Read MoreHow To Get Organized With Reference Managers for Science—ReadCube Papers
Discover more about how ReadCube Papers can help you keep on top of your references.
Read MoreA Protein Renaturation Toolkit: 21 Tips and Tricks for Refolding Proteins
Expressing your protein of interest but not sure if it’s properly folded or struggling with inclusion bodies? Read on to discover advice and tips for battling inclusion bodies and refolding proteins
Read MoreProtein Staining Methods: An Overview of 3 of the Best
There are several great protein staining methods, but how do you pick the one that’s appropriate to your intended application? Read on to find out.
Read MoreHow to Get Organized With Reference Managers for Science—EndNote
Learn more about the key features of EndNote to work out if it’s the right reference manager for you.
Read MoreFlow Cytometry Gone Viral: Introducing Flow Virometry
Do you want to know about a cool way to detect and tell the difference between virus particles? Then read on to discover flow virometry!
Read MoreHow To Get Organized With Reference Managers for Science—Mendeley
Learn how Mendeley allows you to organize a library containing your favorite articles and generate citations and bibliographies while writing papers.
Read MoreTop 10 Best Science Podcasts For Researchers
Facing hours of cell culture, or some other repetitive and mundane lab task? Why not cheer things up with a podcast or two! Discover our top 10 favorite science podcasts for researchers, from Sawbones to The Life Scientific.
Read MoreA Beginner’s Guide to Biocuration—and How to Become a Biocurator
Discover more about biocuration, why it’s so important, and what it takes to become a biocurator. It turns out that biocuration as a career choice is easier than you might think!
Read MoreEthidium Bromide: Understanding the Dangers
This article reassesses the dangers of using ethidium bromide and compares some supposedly safer alternatives
Read MoreScanning Electron Microscopy: 6 SEM Sample Preparation Pointers for Successful Imaging
Discover 6 critical scanning electron microscopy sample preparation points you need to know to get the best out of your SEM.
Read MoreMeltdown: A Hot Introduction to the Thermal Shift Assay
The interaction of proteins with ligands is an important phenomenon. Learn how to detect these interactions (and much more) using the thermal shift assay. In this article, we’ve got you covered with the basic theory and an easy protocol too.
Read MoreProtease Inhibitors 101: How They Work and How to Use Them
Protease inhibitors are a requirement in many lab experiments. In this article, we’ll take you through how protease inhibitors work, why we need them, and how to use them correctly and safely.
Read MoreHomology Modeling in the World of Proteins
Homology modeling is a powerful tool that enables you to predict protein structures. But how do you do it? Read on to find out.
Read MoreUsing Gage R&R Studies to Measure Precision in the Lab
Don’t just take in on faith that your measurements are accurate. Discover how to verify your measurements using gage R&R studies.
Read MoreHow to Make Your Own Chemically Competent Cells
We’ll show you how to make a DIY stock of chemically competent E. coli, the workhorse in the molecular biology laboratory.
Read MoreUsing PubCrawler: How to Speed Up Your Literature Searches by Crawling
PubCrawler automatically searches PubMed and GenBank for queries that you specify, and emails you the results, helping you stay on top of your literature searches.
Read MoreLaemmli Buffer: What Is It for Anyway?
Laemmli buffer is used almost every day by life since researchers. But what do all those components actually do? Find out in this handy article
Read More