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Search results for: real-time PCR

11 qPCR Papers Every Researcher Should Know

…quantification in real-time RT-PCR. Nucleic Acids Res. 29:e45. Lin CH et al. (2011) Quantification bias caused by plasmid DNA conformation in quantitative real-time PCR assay. PLoS One. 6:e29101 Karlen, Y…

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What Is a Cq (Ct) Value?

…though real-time PCR fluorescent dyes and probes should be sequence-specific, a considerable amount of background fluorescence occurs during most real-time PCR experiments. It is critical to bypass or account for…

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An Introduction to MassTag PCR

…is MassTag PCR? MassTag PCR is a type of multiplex PCR that uses mass spectrometry to distinguish between PCR products. To recap briefly, multiplex PCR allows you to detect multiple…

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The A-Z of PCR Variants

PCR. This is used for single cell genetic diagnosis and the identification of high threat bio agents, as it is more efficient and highly specific than conventional PCR. Long-PCR: Using…

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How to Build Your Own PCR Machine

…to a 3D printer to build it. Designed to provide real-time PCR capabilities out in the field or other resource-limiting settings. [1] Arduino PCR Thermocycler $85 The parts needed to…

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What is PCR? The Beginner’s Guide

…making your PCR fail (a bit) less often? Download our free notorious PCR inhibitors poster and pin it up near your DNA engine. Or download the Bitesize Bio PCR eBook…

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The Real-Time PCR Digest

…pathogen loads in clinical samples, and detecting SNPs/mutations. What is Real-time PCR? Real-time PCR, as the name suggests, measures the amount of PCR product at the end of each amplification…

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Amplify Your PCR Success with the Right PCR Instrument!

Nowadays, almost every biology lab has a PCR instrument — from portable, battery-operated machines, to “PCR-by-water baths”, do-it-yourself PCR, or familiar vendor packages, including those with real-time quantification or droplet…

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Quantitative RT-PCR: One-step or Two-step RT?

…am sold on two-step, rather than one-step, qRT-PCR. Are you? References: 1. Spelman, F et al, Analytical Biochemistry, 303, 95-98 (2002). 2. Sigma Aldrich Technical Guide to quantitative PCR: https://www.sigmaaldrich.com/life-science/molecular-biology/pcr/quantitative-pcr/qpcr-technical-guide.html…

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How to Prevent False Results in Colony PCR

…for colony PCR fall into two categories: Insert-specific primers Backbone-specific primers Because many cloning projects begin by PCR amplifying an “insert”, many recommend using these same primers for colony PCR….

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MIQE Guidelines for Digital PCR

…of Quantitative real-time PCR Experiments (MIQE). A recently published update now provides a checklist for digital PCR – Minimum Information for Publication of Quantitative Digital PCR Experiments (dMIQE). MIQE checklist…

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Time to Instigate Nested PCR

…the PCR product. A Simple Solution: Nested PCR Nested PCR is a truly elegant solution. It uses two pairs of primers: the first set bind your target sequence but rather…

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The History of PCR

…the PCR project was back in the oven and over the next few years it was perfected and various applications developed, including DNA fingerprinting (1986), gene amplification systems (1988), real-time

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The Inhibitors Haunting Your PCR

…solve this problem and help you get a seamless PCR. Need help making your PCR fail (a bit) less often? Download our free notorious PCR inhibitors poster and pin it…

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What is Digital PCR?

…choice for you by comparing Digitial PCR with Quantitative Real-Time PCR. Recommended References Huggett, JM and Whale, A (2013) Digital PCR as a Novel Technology and Its Potential Implications for…

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Faster PCR Optimization

…the best results. Using Touchdown PCR One of my favorite tricks is the touchdown PCR method, in which your first few PCR rounds are at a higher annealing temperature. After…

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