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An Introduction to MassTag PCR

…know that PCR’s main purpose is to amplify a stretch of nucleic acids based on sequence-specific primers. Nowadays, a wide range of PCR techniques exist, including qPCR, RACE-PCR, Digital PCR,…

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The A-Z of PCR Variants

…– PCR: R-PCR is very similar to LM-PCR but the linkers are longer and contain an overlapping region with the target sequence. Splicing by overlap/overhang extension (SOE) PCR: This modification…

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What Is a Cq (Ct) Value?

…of quantitative real-time PCR experiments) guidelines recommend using Cq value. Therefore throughout this article, we will use Cq only. [1] What is the Cq Value? Real-time PCR (often called qPCR)

Image of a qPCR plate, to highlight importance of ct value in qPCRRead More

Top Ten websites to help you with your PCR experiments

…can find everything you ever wanted to know about qPCR, including emerging qPCR applications, chemistries, methods and algorithms behind qPCR, Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE),…

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The Real-Time PCR Digest

…pathogen loads in clinical samples, and detecting SNPs/mutations. What is Real-time PCR? Real-time PCR, as the name suggests, measures the amount of PCR product at the end of each amplification…

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Amplify Your PCR Success with the Right PCR Instrument!

Nowadays, almost every biology lab has a PCR instrument — from portable, battery-operated machines, to “PCR-by-water baths”, do-it-yourself PCR, or familiar vendor packages, including those with real-time quantification or droplet…

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What is PCR? – The Beginner’s Guide

PCR is THE technique of modern molecular biology labs. If you need to copy, sequence or quantify DNA , you need to know PCR. In short, PCR (polymerase chain reaction)…

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MIQE Guidelines for Digital PCR

…for publication of real-time PCR experiments (qPCR) suggested by SA Bustin et al. in 2009. MIQE guidelines for qPCR and dPCR publications are divided into the following categories: essential information…

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