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11 qPCR Papers Every Researcher Should Know

…assay design, troubleshooting, and data analysis. qPCR (real-time PCR) protocol explained  Recent and brief YouTube video. A perfect introduction if you are completely new to qPCR.  MIQE qPCR & dPCR

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An Introduction to MassTag PCR

…know that PCR’s main purpose is to amplify a stretch of nucleic acids based on sequence-specific primers. Nowadays, a wide range of PCR techniques exist, including qPCR, RACE-PCR, Digital PCR,…

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The A-Z of PCR Variants

…the vector flanking the insertion site, resulting in amplification of the inserted sequence. Digital PCR (dPCR): This style of PCR allows for more accurate quantification of nucleic acid amounts. Rather…

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What Is a Cq (Ct) Value?

…of quantitative real-time PCR experiments) guidelines recommend using Cq value. Therefore throughout this article, we will use Cq only. [1] What is the Cq Value? Real-time PCR (often called qPCR)

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Top 10 websites to help you with your PCR experiments

…everything you ever wanted to know about qPCR, including emerging qPCR applications, chemistries, methods and algorithms behind qPCR, Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE), cyclers, kits,…

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The Real-Time PCR Digest

…pathogen loads in clinical samples, and detecting SNPs/mutations. What is Real-time PCR? Real-time PCR, as the name suggests, measures the amount of PCR product at the end of each amplification…

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Amplify Your PCR Success with the Right PCR Instrument!

Nowadays, almost every biology lab has a PCR instrument — from portable, battery-operated machines, to “PCR-by-water baths”, do-it-yourself PCR, or familiar vendor packages, including those with real-time quantification or droplet…

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MIQE Guidelines for Digital PCR

…for publication of real-time PCR experiments (qPCR) suggested by SA Bustin et al. in 2009. MIQE guidelines for qPCR and dPCR publications are divided into the following categories: essential information…

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Time to Instigate Nested PCR

…the PCR product. A Simple Solution: Nested PCR Nested PCR is a truly elegant solution. It uses two pairs of primers: the first set bind your target sequence but rather…

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The History of PCR

…the PCR project was back in the oven and over the next few years it was perfected and various applications developed, including DNA fingerprinting (1986), gene amplification systems (1988), real-time

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Overhang PCR

realize you wanted to tag it with something? Or you just want to add restriction enzymes to your PCR product to make it easier to clone into a plasmid? Overhang…

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Touchdown PCR: A Primer and Some Tips

…this article I’ll provide a primer on touchdown PCR (TD-PCR) and some tips and references for perfecting it. So let’s start with… What is touchdown PCR? TD-PCR is a modification…

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What is Digital PCR?

…choice for you by comparing Digitial PCR with Quantitative Real-Time PCR. Recommended References Huggett, JM and Whale, A (2013) Digital PCR as a Novel Technology and Its Potential Implications for…

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Faster PCR Optimization

…the best results. Using Touchdown PCR One of my favorite tricks is the touchdown PCR method, in which your first few PCR rounds are at a higher annealing temperature. After…

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The Inhibitors Haunting Your PCR

…to the PCR Gods. And still, something unknown messes up your results. This unknown at work is generally a PCR inhibitor. Before you blame it all on Murphy’s law and…

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Mediator Probe PCR

…mediator sequence results in dequenching of the UR fluorophore by release of the quencher or strand displacement. Mediator Probe PCR works with both DNA and RNA targets in real-time PCR

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