Search results for: real-time PCR
Polymerase chain reaction-enzyme-linked immunosorbent assay (PCR-ELISA)
…can be designed to amplify the target DNA sequence. Although PCR-ELISA is unlikely to replace conventional ELISA or Real-Time PCR (see Real-Time PCR article) in the detection of proteins, biomolecules…
Read More The Devil is in the Details: How to Setup a PCR Laboratory
…product. Whenever you flick open a tube of amplified PCR product, or pipette PCR product, a small amounts of your PCR product becomes aerosolized. This aerosolized PCR product or amplicon…
Read More An Introduction to MassTag PCR
…know that PCR’s main purpose is to amplify a stretch of nucleic acids based on sequence-specific primers. Nowadays, a wide range of PCR techniques exist, including qPCR, RACE-PCR, Digital PCR,…
Read More The A-Z of PCR Variants
…– PCR: R-PCR is very similar to LM-PCR but the linkers are longer and contain an overlapping region with the target sequence. Splicing by overlap/overhang extension (SOE) PCR: This modification…
Read More What Is a Cq (Ct) Value?
…of quantitative real-time PCR experiments) guidelines recommend using Cq value. Therefore throughout this article, we will use Cq only. [1] What is the Cq Value? Real-time PCR (often called qPCR)…
Read More Entering the Digital Age for Quantitative PCR Analysis: Digital PCR
…PCR success! Quantitative PCR (qPCR) is probably one of the most important PCR spin-offs to date. As the name suggests, qPCR allows you to quantify DNA, and it is not…
Read More Clean Up Your Act! How To Clean Up PCR Contamination
…and avoid it in the future? What is PCR contamination? The biggest source of PCR contamination is aerosolized PCR products, which are created when you open a tube or pipette…
Read More Small Particles (Things) Matter!- Introducing Nanoparticle PCR
There are many different methods and protocols on making your PCR run more efficiently. I recently came across an interesting PCR method called “nanoparticle” PCR. This method seems to attract…
Read More Skip DNA Extraction and Go Direct to PCR with VersaTaq TM Direct PCR Polymerase
…is resistant to many potent PCR inhibitors. This mutant Taq jump starts your setup directly to the PCR step, eliminating costly extraction steps. With the VersaTaq Direct PCR Polymerase protocol,…
Read More Top Ten websites to help you with your PCR experiments
…can find everything you ever wanted to know about qPCR, including emerging qPCR applications, chemistries, methods and algorithms behind qPCR, Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE),…
Read More The Real-Time PCR Digest
…pathogen loads in clinical samples, and detecting SNPs/mutations. What is Real-time PCR? Real-time PCR, as the name suggests, measures the amount of PCR product at the end of each amplification…
Read More Digital PCR or Quantitative Real-Time PCR: Which Method Is Best for Your Quantitative PCR Application?
So you’re designing a new experiment that requires PCR quantification. You used to have only one method to choose from, but now you have two – Quantitative Real-Time PCR (qPCR)…
Read More Amplify Your PCR Success with the Right PCR Instrument!
Nowadays, almost every biology lab has a PCR instrument — from portable, battery-operated machines, to “PCR-by-water baths”, do-it-yourself PCR, or familiar vendor packages, including those with real-time quantification or droplet…
Read More Photonic PCR: When Lightening Strikes Your DNA
…you learn about photonic PCR. What the Heck is Photonic PCR “So what is it?” Well, the conventional PCR uses “hotplates” to regulate the temperature inside a reaction tube. It…
Read More What is PCR? – The Beginner’s Guide
PCR is THE technique of modern molecular biology labs. If you need to copy, sequence or quantify DNA , you need to know PCR. In short, PCR (polymerase chain reaction)…
Read More Decisions, Decisions: How to Choose the Best qPCR Probe for Your Experiment
…ALL. THE. TIME.) called “real time PCR” and shortened to “RT-PCR”. Oh, biologists! RT-qPCR, or reverse-transcriptase quantitative PCR, is, as you probably guessed, a combination of the two methods mentioned…
Read More MIQE Guidelines for Digital PCR
…for publication of real-time PCR experiments (qPCR) suggested by SA Bustin et al. in 2009. MIQE guidelines for qPCR and dPCR publications are divided into the following categories: essential information…
Read More Divide and Conquer: How to Setup Your First Droplet Digital PCR Experiment
…In a nutshell, digital PCR is an end-point PCR technology that divides a single PCR into a large number of partitions, and then perform PCR inside of each partition. Upon…
Read More 8 Essential Papers and Reference Guides for Quantitative PCR (qPCR)
…especially true for techniques that have transformed scientific research. Quantitative PCR (qPCR) also known as Real-Time PCR (RT-PCR) has revolutionized the study of gene expression by allowing the ‘real-time’ detection…
Read More How to Be Greener – The Environmentally Friendly Guide to PCR
Science is an expensive business and those who use high energy-demanding techniques may not even realize just how expensive they are. The Cost of PCR Let’s looks at PCR. You…
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